##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956094 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.634667352386952 33.0 31.0 34.0 30.0 34.0 2 31.73360533798478 33.0 31.0 34.0 30.0 34.0 3 31.760071346264546 33.0 31.0 34.0 30.0 34.0 4 35.34709374907341 37.0 35.0 37.0 33.0 37.0 5 35.33765657478628 37.0 35.0 37.0 33.0 37.0 6 35.38982993659814 37.0 35.0 37.0 33.0 37.0 7 35.32295840588438 37.0 35.0 37.0 33.0 37.0 8 35.28574086930383 37.0 35.0 37.0 33.0 37.0 9 36.890412219453665 39.0 37.0 39.0 33.0 39.0 10-11 36.7543471837243 39.0 37.0 39.0 32.5 39.0 12-13 36.654806210744475 39.0 37.0 39.0 32.0 39.0 14-15 37.81349055822471 40.0 38.0 41.0 32.5 41.0 16-17 37.734659735166105 40.0 38.0 41.0 32.0 41.0 18-19 37.65824392897274 40.0 37.0 41.0 32.0 41.0 20-21 37.57600222688685 40.0 37.0 41.0 32.0 41.0 22-23 37.46234690152927 40.0 37.0 41.0 32.0 41.0 24-25 37.42238997716879 40.0 37.0 41.0 32.0 41.0 26-27 37.44181465716883 40.0 37.0 41.0 32.0 41.0 28-29 37.35673157833928 40.0 37.0 41.0 31.5 41.0 30-31 37.20858660166638 40.0 37.0 41.0 31.0 41.0 32-33 37.024626372761226 40.0 36.0 41.0 31.0 41.0 34-35 36.86274611547297 39.0 36.0 41.0 30.0 41.0 36-37 36.69300452841223 39.0 36.0 41.0 30.0 41.0 38-39 36.5370984216505 39.0 35.5 41.0 30.0 41.0 40-41 36.345293988939176 39.0 35.0 41.0 30.0 41.0 42-43 36.129901988350255 39.0 35.0 40.5 29.5 41.0 44-45 35.88564787786272 39.0 35.0 40.0 28.5 41.0 46-47 35.5661284682638 38.5 35.0 40.0 27.0 41.0 48-49 35.34738616855836 38.5 35.0 40.0 26.0 41.0 50-51 34.662559672490175 38.0 34.0 39.5 25.0 40.5 52-53 34.84163567804001 38.0 34.5 40.0 26.0 40.5 54-55 35.20539631531 39.0 35.0 40.0 26.0 41.0 56-57 35.06854987541499 38.5 35.0 40.0 26.0 41.0 58-59 34.82852511177888 38.0 34.0 40.0 26.0 41.0 60-61 34.51533643066233 38.0 34.0 40.0 25.5 41.0 62-63 34.18255589966535 37.0 34.0 40.0 25.0 41.0 64-65 33.824660522449335 37.0 33.5 40.0 23.5 41.0 66-67 33.44045966093654 36.0 33.0 39.0 23.0 41.0 68-69 33.0340361966245 36.0 33.0 39.0 22.0 41.0 70-71 32.608717934823176 35.0 32.0 38.5 21.5 40.0 72-73 32.222039687254295 35.0 32.0 37.5 20.0 40.0 74-75 31.826049770614297 35.0 32.0 37.0 19.5 39.0 76-77 31.38295910114749 35.0 31.0 36.5 18.0 39.0 78-79 30.944316581922955 35.0 31.0 36.0 16.0 38.5 80-81 30.53716079084134 34.5 31.0 35.5 11.5 37.0 82-83 30.15832879197012 34.0 30.0 35.0 7.5 37.0 84-85 29.80917455909583 34.0 30.0 35.0 7.0 36.5 86-87 29.470874865931798 34.0 30.0 35.0 2.0 36.0 88-89 29.124165556460987 34.0 29.0 35.0 2.0 36.0 90-91 28.771861168226067 34.0 29.0 35.0 2.0 35.0 92-93 28.393161831691117 34.0 29.0 35.0 2.0 35.0 94-95 28.019432603954616 34.0 29.0 35.0 2.0 35.0 96-97 27.51650150759626 34.0 27.0 35.0 2.0 35.0 98-99 26.931370373816392 34.0 26.5 35.0 2.0 35.0 100-101 24.75268392009791 31.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29954668704691656 1101 2 0.28574954561603505 1101 3 0.23579815012039873 1101 4 0.3250392343310651 1101 5 0.31091851371754586 1101 6 0.35620686670813484 1101 7 0.19385047815262624 1101 8 0.21441012220330435 1101 9 0.21298642090323483 1101 10-11 0.2647026467466844 1101 12-13 0.4190024416336726 1101 14-15 0.5194198675120205 1101 16-17 0.4850848057202981 1101 18-19 0.4241260627473338 1101 20-21 0.3666293352387058 1101 22-23 0.3965693437119029 1101 24-25 0.5116091892740755 1101 26-27 0.4203473376695612 1101 28-29 0.39245239004504384 1101 30-31 0.3825591192263218 1101 32-33 0.13223583998991728 1101 34-35 0.2834909347630159 1101 36-37 0.3865933227953775 1101 38-39 0.3041299611957271 1101 40-41 0.28841289470504705 1101 42-43 0.2399725926587024 1101 44-45 0.29535092988211176 1101 46-47 0.42415219919288205 1101 48-49 0.36860430376814435 1101 50-51 0.38299485704113323 1101 52-53 0.3425389700775412 1101 54-55 0.39350474783812217 1101 56-57 0.47091323782072436 1101 58-59 0.5237227376228404 1101 60-61 0.5325509701047437 1101 62-63 0.475751901298473 1101 64-65 0.3886200490213483 1101 66-67 0.42894998718155364 1101 68-69 0.40107156946364597 1101 70-71 0.4390807635259648 1101 72-73 0.3196153293691921 1101 74-75 0.30892049359342977 1101 76-77 0.3365339186923926 1101 78-79 0.39514536889813456 1101 80-81 0.367999028746933 1101 82-83 0.13490892687316958 1101 84-85 0.12264281968113977 1101 86-87 0.21974297865927994 1101 88-89 0.3065623081070008 1101 90-91 0.44643819933740403 1101 92-93 0.4371685732411983 1101 94-95 0.4641208815386868 1101 96-97 0.3730837089552743 1101 98-99 0.4350558042234667 1101 100-101 0.6909182041344053 1102 1 0.1871345851690016 1102 2 0.24273320758006278 1102 3 0.26096715372236545 1102 4 0.30813328019392117 1102 5 0.25838341973135215 1102 6 0.12330820920061569 1102 7 0.09325437718253227 1102 8 0.10037790856378592 1102 9 0.23385448328473046 1102 10-11 0.21499275916358584 1102 12-13 0.24314146757411237 1102 14-15 0.1441804601713912 1102 16-17 0.36386091543824506 1102 18-19 0.1669795371971361 1102 20-21 0.15829633487802397 1102 22-23 0.19446532944681394 1102 24-25 0.2316958580611086 1102 26-27 0.20201823175708 1102 28-29 0.16405152719301697 1102 30-31 0.03564023509494518 1102 32-33 0.12204980683436872 1102 34-35 0.13941339954915577 1102 36-37 0.25255664465846905 1102 38-39 0.3337765147156233 1102 40-41 0.1177383027563792 1102 42-43 0.22468728303941532 1102 44-45 0.24364160808615054 1102 46-47 0.38867942330372074 1102 48-49 0.32783273419897085 1102 50-51 0.19073484641797478 1102 52-53 0.40194262403957737 1102 54-55 0.23975369098290145 1102 56-57 0.3382825034322181 1102 58-59 0.22412432928541648 1102 60-61 0.22018452543760247 1102 62-63 0.09557091334462342 1102 64-65 0.34959618633607903 1102 66-67 0.1359513632909497 1102 68-69 0.27492128212156075 1102 70-71 0.24777465297158585 1102 72-73 0.1863189160441081 1102 74-75 0.33386520681340315 1102 76-77 0.26327220910954807 1102 78-79 0.1695691796929495 1102 80-81 0.2026519993158793 1102 82-83 0.22276123192300545 1102 84-85 0.20745654944905212 1102 86-87 0.1952697547221156 1102 88-89 0.32790612182131795 1102 90-91 0.07460044066426619 1102 92-93 0.16395252086912748 1102 94-95 0.33746527489180167 1102 96-97 0.1895252061921333 1102 98-99 0.320015349377492 1102 100-101 0.29366903776475084 1103 1 0.28427577940981763 1103 2 0.22528659366434312 1103 3 0.1622254162668817 1103 4 0.2559782053402344 1103 5 0.1556276215152721 1103 6 0.19419171829345316 1103 7 0.2663116687770213 1103 8 0.30124030803904844 1103 9 0.20074194638908693 1103 10-11 0.273340989327707 1103 12-13 0.3217874957787714 1103 14-15 0.5300811294894601 1103 16-17 0.6098168300574613 1103 18-19 0.45180116785735436 1103 20-21 0.4548980097899218 1103 22-23 0.40999937505652895 1103 24-25 0.44602429969094004 1103 26-27 0.5005426371541262 1103 28-29 0.4981816677544302 1103 30-31 0.42249360870746244 1103 32-33 0.4110021323566784 1103 34-35 0.5326724103995986 1103 36-37 0.44554912926507484 1103 38-39 0.4090528567006686 1103 40-41 0.48031677094645886 1103 42-43 0.41126604685651813 1103 44-45 0.5274239249706056 1103 46-47 0.5276633901565404 1103 48-49 0.5484963706885893 1103 50-51 0.4415108673705461 1103 52-53 0.5188273156405288 1103 54-55 0.6164645169371141 1103 56-57 0.7067377136276534 1103 58-59 0.5933636439850147 1103 60-61 0.6396804351604999 1103 62-63 0.5332663620741158 1103 64-65 0.6386847774423217 1103 66-67 0.6420925529125938 1103 68-69 0.5318165516271804 1103 70-71 0.4223724385403642 1103 72-73 0.37137681736935235 1103 74-75 0.2852749782105626 1103 76-77 0.19183786371555556 1103 78-79 0.4191361322863827 1103 80-81 0.4858442169343249 1103 82-83 0.39978985693397817 1103 84-85 0.25909774138028396 1103 86-87 0.44231348442662366 1103 88-89 0.3094583023016213 1103 90-91 0.3154718013338602 1103 92-93 0.3521087320392091 1103 94-95 0.45330141437232996 1103 96-97 0.45901233948758957 1103 98-99 0.48018053098024893 1103 100-101 0.5074177732663223 1104 1 0.3062106844928678 1104 2 0.18078524780126415 1104 3 0.2231599151815331 1104 4 0.2793703890080579 1104 5 0.24867225040019036 1104 6 0.11610973894536158 1104 7 0.1891646738615549 1104 8 0.26668936325366843 1104 9 0.3372922469573254 1104 10-11 0.4926935728449493 1104 12-13 0.2431091264380072 1104 14-15 0.20336095560156053 1104 16-17 0.46298577690738085 1104 18-19 0.37774636084894553 1104 20-21 0.36735169083141983 1104 22-23 0.2893535020861435 1104 24-25 0.4252779172527781 1104 26-27 0.3792974755762799 1104 28-29 0.40093332277289306 1104 30-31 0.5469354894881562 1104 32-33 0.31786619710593556 1104 34-35 0.3262061624136763 1104 36-37 0.2842105090999212 1104 38-39 0.4476865004152586 1104 40-41 0.3432844850879633 1104 42-43 0.34221461129942554 1104 44-45 0.3473783696159458 1104 46-47 0.2734253435910219 1104 48-49 0.34427401287560855 1104 50-51 0.3222412929342937 1104 52-53 0.2933918078267723 1104 54-55 0.576863369955241 1104 56-57 0.45255238561858846 1104 58-59 0.4376503293066776 1104 60-61 0.49951311624511874 1104 62-63 0.40060971640188114 1104 64-65 0.33191495390637016 1104 66-67 0.14071190645628207 1104 68-69 0.21575195744592435 1104 70-71 0.2658769507777734 1104 72-73 0.34400501676071116 1104 74-75 0.3317924012405662 1104 76-77 0.38564259997965067 1104 78-79 0.43754269851846317 1104 80-81 0.35468497754533956 1104 82-83 0.4135551068181229 1104 84-85 0.463825726310386 1104 86-87 0.5703481076556152 1104 88-89 0.3564831640829844 1104 90-91 0.30071984485946146 1104 92-93 0.4184999638744067 1104 94-95 0.689641148692548 1104 96-97 0.6803887901990002 1104 98-99 0.7057431249361343 1104 100-101 0.6795557263152858 1105 1 0.25263996986645054 1105 2 0.23089863555204104 1105 3 0.2438105256344052 1105 4 0.32820606091532767 1105 5 0.3322063406436371 1105 6 0.2788419420893504 1105 7 0.34069515280508966 1105 8 0.37592579275289495 1105 9 0.38992071355884406 1105 10-11 0.3871216271780895 1105 12-13 0.3859634666458831 1105 14-15 0.5081061330192185 1105 16-17 0.629635831346512 1105 18-19 0.4561071988862295 1105 20-21 0.4802121583478467 1105 22-23 0.4749112777708646 1105 24-25 0.24923234644145253 1105 26-27 0.3793533558864226 1105 28-29 0.3512980324509698 1105 30-31 0.4385548376503863 1105 32-33 0.29765230847790036 1105 34-35 0.1923538519348682 1105 36-37 0.27741551917418406 1105 38-39 0.41946592102662805 1105 40-41 0.38700873574788375 1105 42-43 0.36486603438775234 1105 44-45 0.31867058086768907 1105 46-47 0.35897372840831565 1105 48-49 0.40124078129216656 1105 50-51 0.1846567170156419 1105 52-53 0.43281662184575254 1105 54-55 0.4806693676512239 1105 56-57 0.5468629146405561 1105 58-59 0.4433599767028511 1105 60-61 0.4950392512151325 1105 62-63 0.37419497292597015 1105 64-65 0.5311342102054581 1105 66-67 0.47181734172298206 1105 68-69 0.5230858529915849 1105 70-71 0.3967911284771617 1105 72-73 0.5523685604835649 1105 74-75 0.6359886313186784 1105 76-77 0.6396776511598823 1105 78-79 0.603715027444288 1105 80-81 0.48077401598560243 1105 82-83 0.5563464073416853 1105 84-85 0.5509922226707289 1105 86-87 0.8229154871294106 1105 88-89 0.7201498050254962 1105 90-91 0.5309402141158053 1105 92-93 0.4898374558433538 1105 94-95 0.5764292209892083 1105 96-97 0.5596531970448488 1105 98-99 0.7315575037488458 1105 100-101 0.7741957725071948 1106 1 0.2766680025712738 1106 2 0.23965155562545704 1106 3 0.2531172167740685 1106 4 0.16045305741123883 1106 5 0.16715023351865455 1106 6 0.31337493891898305 1106 7 0.1953849992926635 1106 8 0.27611351175840326 1106 9 0.4088938905987263 1106 10-11 0.36268217698619765 1106 12-13 0.31970856509407497 1106 14-15 0.1467971058208164 1106 16-17 0.35156714704816494 1106 18-19 0.4323273724220229 1106 20-21 0.2263107316834052 1106 22-23 0.06077864386463716 1106 24-25 0.15348678912457814 1106 26-27 0.14485529564947797 1106 28-29 0.1605390219387104 1106 30-31 0.33818200684778077 1106 32-33 0.2728707925426477 1106 34-35 0.40665804015445417 1106 36-37 0.2567706001019303 1106 38-39 0.21929958113008752 1106 40-41 0.4981686306252371 1106 42-43 0.3298522683273504 1106 44-45 0.20896185819123048 1106 46-47 0.24768399455214762 1106 48-49 0.36945473924311045 1106 50-51 0.44613364843562664 1106 52-53 0.5034479834342704 1106 54-55 0.4930468288559595 1106 56-57 0.3892900170934155 1106 58-59 0.5086267462673462 1106 60-61 0.615678955870564 1106 62-63 0.7092780907300735 1106 64-65 0.4321072203839904 1106 66-67 0.35070677522873694 1106 68-69 0.5685399244082419 1106 70-71 0.5243430640686881 1106 72-73 0.5211654902349494 1106 74-75 0.6962771971454806 1106 76-77 0.5950923015853782 1106 78-79 0.4628068846109805 1106 80-81 0.6801780081431161 1106 82-83 0.47374378761867675 1106 84-85 0.44337500258062335 1106 86-87 0.6417244100394761 1106 88-89 0.7906977359448994 1106 90-91 0.9114935680287068 1106 92-93 1.1099067032987193 1106 94-95 1.2223551971280386 1106 96-97 1.1654631002652742 1106 98-99 1.1774042207520168 1106 100-101 1.4656780226323427 1107 1 0.3516451087983796 1107 2 0.348534339508241 1107 3 0.42198168563853855 1107 4 0.36430684251502754 1107 5 0.30407025793867604 1107 6 0.2901988094928498 1107 7 0.30771711162477544 1107 8 0.26278170467659834 1107 9 0.2860916927965249 1107 10-11 0.364578544507566 1107 12-13 0.5813500486105596 1107 14-15 0.5500823622310804 1107 16-17 0.6116747477807678 1107 18-19 0.681682531152191 1107 20-21 0.6774592359569098 1107 22-23 0.7148651823261787 1107 24-25 0.7231563556691469 1107 26-27 0.7501933603625446 1107 28-29 0.6124773613770458 1107 30-31 0.7244419274827081 1107 32-33 0.7862178621397149 1107 34-35 0.5737902135366255 1107 36-37 0.5948238662802012 1107 38-39 0.8053191171496223 1107 40-41 0.8321353583419651 1107 42-43 0.877814722539803 1107 44-45 0.888967963197338 1107 46-47 0.8314065220246718 1107 48-49 0.995462065250436 1107 50-51 0.951971560523539 1107 52-53 0.9657915426528376 1107 54-55 0.9728735993077322 1107 56-57 1.0625348278382276 1107 58-59 0.8818105069511049 1107 60-61 1.0788695846861884 1107 62-63 1.1294246108765975 1107 64-65 1.1258495891263678 1107 66-67 1.0880045469015087 1107 68-69 1.1278882150065286 1107 70-71 0.9841225207231403 1107 72-73 1.0225696416391017 1107 74-75 0.9615511592944337 1107 76-77 0.8815964224645008 1107 78-79 0.7684158589699557 1107 80-81 0.7665218726119818 1107 82-83 0.8609041966540865 1107 84-85 0.8285818096624311 1107 86-87 0.9138092696243341 1107 88-89 0.8251757579229242 1107 90-91 0.8397215900067287 1107 92-93 0.8903897619067784 1107 94-95 0.9781587685566073 1107 96-97 0.9808744250515993 1107 98-99 1.2327003135481114 1107 100-101 1.3678185903979099 1108 1 -0.11048446932696265 1108 2 -0.010715573237433773 1108 3 0.05693958542694588 1108 4 0.03722219127782722 1108 5 -0.08576411335755552 1108 6 -0.014073834822163178 1108 7 -0.07754458772591022 1108 8 -0.016006541802326524 1108 9 -0.18213887130268347 1108 10-11 -0.09597045788465408 1108 12-13 0.01774573606557084 1108 14-15 0.11690792720283127 1108 16-17 0.0942641016624961 1108 18-19 0.14707198992088166 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-2.7518494670202003 2316 70-71 -2.359064211629885 2316 72-73 -2.6687115985649434 2316 74-75 -2.9372922051009915 2316 76-77 -2.921516822384607 2316 78-79 -3.254792549168883 2316 80-81 -3.214338604433415 2316 82-83 -3.6852806335017974 2316 84-85 -3.4747647571210756 2316 86-87 -3.8485608003366245 2316 88-89 -3.8326130981940274 2316 90-91 -4.272043178737395 2316 92-93 -4.225370422328496 2316 94-95 -4.36760029359608 2316 96-97 -4.577383479396836 2316 98-99 -4.616403178087921 2316 100-101 -5.058791865274486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5794.0 3 2198.0 4 2118.0 5 2400.0 6 2492.0 7 2847.0 8 3318.0 9 4093.0 10 4730.0 11 5871.0 12 6859.0 13 8105.0 14 8944.0 15 10421.0 16 13049.0 17 16648.0 18 15179.0 19 10692.0 20 10859.0 21 11819.0 22 12756.0 23 14057.0 24 15954.0 25 18605.0 26 21720.0 27 25447.0 28 30302.0 29 36490.0 30 44781.0 31 55384.0 32 71212.0 33 93106.0 34 127716.0 35 182708.0 36 278322.0 37 400100.0 38 326277.0 39 52695.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.269540185505 18.051169595876896 14.348433762789458 23.33085645582865 2 15.628878378647881 19.407093543360883 40.39425165157886 24.569776426412375 3 19.519859032927428 23.87905916955831 29.220600582726625 27.380481214787633 4 13.248838498630338 16.130446167914943 36.341132594254994 34.279582739199725 5 14.692848141001729 36.40549151921423 33.488627217415015 15.413033122369022 6 32.77763050017861 34.83389304507411 17.48398910532441 14.90448734942287 7 29.197667578834302 31.954834914708062 20.4438249661134 18.40367254034424 8 26.783490491222867 34.36083724309418 19.401896127418127 19.453776138264832 9 26.568702883663065 16.71269163713156 19.878029565117743 36.84057591408763 10-11 25.394156228009884 26.110373719729257 26.88800686136357 21.607463190897285 12-13 26.22280040444335 23.28242663964921 27.595866656469468 22.898906299437968 14-15 23.334121097816308 24.52011587299395 25.69235034648849 26.453412682701256 16-17 22.931154723431504 27.812133256578015 26.09554707473491 23.16116494525557 18-19 22.874344092819882 26.470720630857635 28.33040739137952 22.32452788494296 20-21 23.619382668657725 25.54580345065781 28.434411645784703 22.40040223489976 22-23 23.421357171823168 25.35838788479427 28.345511847907407 22.874743095475157 24-25 23.099422828859478 25.64617962195776 28.35664462587663 22.89775292330613 26-27 23.004722557721923 25.867039870145636 28.211677945847153 22.91655962628528 28-29 22.853217141900366 25.820981176834483 28.411157138347136 22.91464454291802 30-31 23.038571614081405 25.685660210286514 28.232365268267145 23.043402907364936 32-33 22.886400446631246 25.77498394663329 28.284881818590822 23.05373378814464 34-35 23.17459635787127 25.794164178772544 28.112653993169122 22.918585470187068 36-37 23.230640749270123 25.70765550936396 28.008160600536762 23.053543140829152 38-39 23.09298383693326 25.7658930688048 28.118281205306555 23.022841888955377 40-41 23.208805681289206 25.703738488096285 28.103015162085 22.984440668529516 42-43 23.069712632219343 25.820871868018386 27.906090397386542 23.20332510237573 44-45 23.068523124349777 25.937205242183886 27.798655968262654 23.19561566520369 46-47 23.25440807333071 25.995337590168038 27.48117398661602 23.26908034988523 48-49 23.328290105483468 26.1711062664087 27.288105829567527 23.2124977985403 50-51 23.232506552709754 26.321520647873452 27.18982178981126 23.256151009605528 52-53 23.356278259375824 26.34221171091029 27.00857571723344 23.292934312480444 54-55 23.222879300203005 26.240623473354596 27.04944036742512 23.487056859017276 56-57 23.099119733836755 26.542140170275967 27.085714431789278 23.273025664098 58-59 23.3337048396192 26.266499863752625 27.103948310864872 23.295846985763298 60-61 23.278026435861975 26.14008808545772 27.332871519461367 23.24901395921894 62-63 23.198637783212533 26.2163334619167 27.389228497243895 23.19580025762687 64-65 23.16587403935743 26.25171423495595 27.231393920648657 23.351017805037966 66-67 23.082187770763234 26.436641435272357 27.044071466026075 23.43709932793833 68-69 23.01790274789753 26.304833862121047 27.30217001997434 23.375093370007086 70-71 23.059065800218566 26.194053650797954 27.14823264995986 23.59864789902362 72-73 23.07231208543229 26.363163931404888 26.99234699289913 23.572176990263692 74-75 23.035408195742736 26.597241839472453 26.867663769775785 23.49968619500903 76-77 23.21312380716195 26.467946611988395 26.821071416244713 23.49785816460494 78-79 23.145311373845225 26.438748813362846 26.836829944700728 23.5791098680912 80-81 23.070152574771203 26.58211691388116 26.809691179904917 23.53803933144272 82-83 23.177810475772873 26.510560017794983 26.833221901634147 23.478407604797997 84-85 23.176938737738293 26.482627310589677 26.639180402129774 23.701253549542255 86-87 23.1322021660096 26.383850119519934 26.862134412051148 23.621813302419316 88-89 23.163359976432414 26.260875969452158 26.77695388977654 23.79881016433889 90-91 23.144816010150954 26.366367656471475 26.878364759706326 23.61045157367125 92-93 23.13399688180124 26.311342710091644 26.795412764975936 23.75924764313118 94-95 23.14521254213596 26.253750886433718 26.752045508113774 23.84899106331655 96-97 23.061098229099823 26.251479584923697 26.839478777063942 23.847943408912535 98-99 22.931672336517043 26.281597568135375 26.771708912677482 24.0150211826701 100-101 22.976103323925273 26.289362037379515 26.728890826093117 24.00564381260209 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 183.0 1 148.5 2 153.0 3 199.5 4 201.5 5 212.5 6 354.0 7 753.0 8 1325.0 9 1660.0 10 1596.0 11 1515.5 12 1592.0 13 1719.5 14 1837.0 15 2004.5 16 2168.5 17 2310.5 18 2478.0 19 2640.0 20 2916.5 21 3255.5 22 3849.5 23 4682.5 24 5640.5 25 7052.0 26 8909.5 27 10937.5 28 12828.5 29 15111.5 30 17938.0 31 20618.5 32 23268.0 33 26192.0 34 29421.5 35 32221.0 36 35816.0 37 39793.0 38 43418.5 39 46423.0 40 48835.5 41 52152.0 42 55941.0 43 59810.5 44 63679.0 45 67535.5 46 71019.5 47 74346.0 48 78254.0 49 80464.5 50 79704.0 51 78682.0 52 77955.0 53 77739.5 54 77098.5 55 73771.5 56 68699.0 57 62339.5 58 56711.5 59 50760.0 60 43389.5 61 35935.0 62 28572.5 63 22102.0 64 16367.0 65 11898.0 66 8488.0 67 6283.0 68 4405.0 69 2846.5 70 1899.5 71 1162.0 72 742.0 73 444.0 74 263.5 75 168.5 76 102.5 77 65.0 78 46.0 79 28.0 80 16.0 81 20.0 82 21.0 83 18.0 84 13.5 85 7.5 86 7.0 87 6.5 88 5.0 89 4.0 90 2.0 91 2.5 92 3.0 93 2.5 94 2.0 95 2.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03450754411597807 2 0.027657157580361677 3 0.02351625228644431 4 0.04427190104361038 5 0.08869716894995844 6 0.10797027136732693 7 0.05337166823271275 8 0.0809777035254952 9 0.06942406653258995 10-11 0.07057431800312255 12-13 0.06689351329741822 14-15 0.061091133657175986 16-17 0.049256324082584985 18-19 0.059710831892536856 20-21 0.05201692761186323 22-23 0.030724494835115284 24-25 0.008946400326364684 26-27 0.002888409248226312 28-29 0.006466969378772185 30-31 0.004907739607605769 32-33 0.006032429934348759 34-35 0.006262480228455279 36-37 0.005649012777504557 38-39 0.003092898398543219 40-41 0.006620336241509867 42-43 0.003271826405070513 44-45 0.001993769215589844 46-47 0.0012269349019014423 48-49 2.811725816857472E-4 50-51 0.0016870354901144832 52-53 0.005418962483398037 54-55 0.010863486110585687 56-57 0.007745026568252855 58-59 0.004754372744868089 60-61 0.001993769215589844 62-63 0.008409616306782802 64-65 0.0023771863724340444 66-67 0.020218864737584184 68-69 0.042712671272443964 70-71 0.05431743055292844 72-73 0.003348509836439353 74-75 0.013368478201967798 76-77 0.021036821338851815 78-79 0.02364405800539238 80-81 0.06888728251300807 82-83 0.02487099290729382 84-85 0.010633435816479167 86-87 0.028756286763315056 88-89 0.04427190104361038 90-91 0.041383491795384066 92-93 0.04082114663201257 94-95 0.01140027013016757 96-97 0.01579678686198107 98-99 0.036910291632201726 100-101 0.07090661287238753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1956094.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.756805956594896 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22114256956719 47.52466780802338 2 12.431892712983773 15.106441864719223 3 3.9932899647081785 7.278586305425774 4 1.7202115986674982 4.180582492181003 5 0.9464660771305311 2.8752127896359627 6 0.5934974328260831 2.163540501717093 7 0.42279162627761074 1.7981228158475386 8 0.2838547806336719 1.3796887861449452 9 0.21229868891989148 1.1608731222790816 >10 1.1053510401465985 11.797859189381223 >50 0.050926128838580526 2.097466451541753 >100 0.017688110116312716 1.7524410864796853 >500 2.52543936127388E-4 0.10142227291975034 >1k 2.525439361273878E-4 0.4582670526278177 >5k 8.41813120424626E-5 0.3248274610758114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6351 0.3246776484156692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4628 0.23659394691666144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2999 0.15331574045010107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1122287579226765E-5 5.1122287579226765E-5 5 5.1122287579226765E-5 0.0 0.0 5.1122287579226765E-5 5.1122287579226765E-5 6 1.0224457515845353E-4 5.1122287579226765E-5 0.0 5.1122287579226765E-5 5.1122287579226765E-5 7 1.0224457515845353E-4 5.1122287579226765E-5 0.0 5.1122287579226765E-5 5.1122287579226765E-5 8 1.0224457515845353E-4 5.1122287579226765E-5 0.0 5.1122287579226765E-5 5.1122287579226765E-5 9 1.0224457515845353E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 5.1122287579226765E-5 10-11 1.0224457515845353E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 5.1122287579226765E-5 12-13 1.0224457515845353E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 5.1122287579226765E-5 14-15 1.0224457515845353E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 7.668343136884014E-5 16-17 1.0224457515845353E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 1.2780571894806692E-4 18-19 1.5336686273768028E-4 5.1122287579226765E-5 0.0 1.0224457515845353E-4 1.533668627376803E-4 20-21 2.0448915031690706E-4 5.1122287579226765E-5 0.0 1.789280065272937E-4 1.533668627376803E-4 22-23 2.0448915031690706E-4 5.1122287579226765E-5 0.0 2.5561143789613384E-4 1.533668627376803E-4 24-25 2.0448915031690706E-4 5.1122287579226765E-5 0.0 3.067337254753606E-4 1.533668627376803E-4 26-27 2.0448915031690706E-4 7.668343136884014E-5 0.0 4.089783006338141E-4 1.533668627376803E-4 28-29 2.0448915031690706E-4 1.0224457515845353E-4 0.0 6.390285947403345E-4 1.533668627376803E-4 30-31 2.0448915031690706E-4 1.0224457515845353E-4 0.0 0.001329179477059896 1.533668627376803E-4 32-33 2.0448915031690706E-4 1.0224457515845353E-4 0.0 0.002274941797275591 2.0448915031690706E-4 34-35 2.5561143789613384E-4 1.0224457515845353E-4 0.0 0.003348509836439353 2.5561143789613384E-4 36-37 3.067337254753606E-4 1.0224457515845353E-4 0.0 0.004345394444234275 2.5561143789613384E-4 38-39 3.067337254753606E-4 1.0224457515845353E-4 0.0 0.005725696208873398 2.5561143789613384E-4 40-41 3.067337254753606E-4 1.0224457515845353E-4 0.0 0.009074206045312751 2.5561143789613384E-4 42-43 3.067337254753606E-4 1.0224457515845353E-4 0.0 0.01224378787522481 2.5561143789613384E-4 44-45 3.5785601305458734E-4 1.0224457515845353E-4 0.0 0.01934978584873733 2.5561143789613384E-4 46-47 3.5785601305458734E-4 1.0224457515845353E-4 0.0 0.034712033266294975 2.811725816857472E-4 48-49 3.5785601305458734E-4 1.0224457515845353E-4 0.0 0.04728811601078476 3.067337254753606E-4 50-51 3.5785601305458734E-4 1.0224457515845353E-4 0.0 0.061065572513386374 3.067337254753606E-4 52-53 3.5785601305458734E-4 1.0224457515845353E-4 0.0 0.07839602800274424 3.32294869264974E-4 54-55 4.089783006338141E-4 1.0224457515845353E-4 0.0 0.098180353295905 3.5785601305458734E-4 56-57 4.601005882130409E-4 1.2780571894806692E-4 0.0 0.12141543300066357 3.5785601305458734E-4 58-59 4.601005882130409E-4 1.533668627376803E-4 0.0 0.15865801950213027 3.5785601305458734E-4 60-61 4.601005882130409E-4 1.533668627376803E-4 0.0 0.20126844619941578 3.5785601305458734E-4 62-63 4.601005882130409E-4 1.533668627376803E-4 0.0 0.2540522081249674 3.5785601305458734E-4 64-65 4.601005882130409E-4 1.533668627376803E-4 0.0 0.29796625315552316 3.8341715684420076E-4 66-67 5.112228757922677E-4 1.533668627376803E-4 0.0 0.3635561481196712 4.089783006338141E-4 68-69 5.112228757922677E-4 1.533668627376803E-4 0.0 0.44609308141633275 4.089783006338141E-4 70-71 5.879063071611078E-4 1.533668627376803E-4 0.0 0.5095869625897325 4.089783006338141E-4 72-73 6.134674509507212E-4 1.533668627376803E-4 0.0 0.6078695604607958 4.601005882130409E-4 74-75 6.134674509507212E-4 1.533668627376803E-4 0.0 0.6921446515351513 4.601005882130409E-4 76-77 6.134674509507212E-4 1.533668627376803E-4 0.0 0.7702083846686305 4.601005882130409E-4 78-79 6.134674509507212E-4 1.533668627376803E-4 0.0 0.8680308819514808 4.601005882130409E-4 80-81 6.64589738529948E-4 1.533668627376803E-4 0.0 0.9633995094305284 4.856617320026543E-4 82-83 7.668343136884015E-4 1.533668627376803E-4 0.0 1.0899016100453252 5.623451633714944E-4 84-85 7.668343136884015E-4 1.533668627376803E-4 0.0 1.2586307202005629 5.623451633714944E-4 86-87 7.668343136884015E-4 1.533668627376803E-4 0.0 1.47699957159523 5.623451633714944E-4 88-89 7.668343136884015E-4 1.533668627376803E-4 0.0 1.7414807263863596 5.623451633714944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3720 0.0 51.883602 1 GTATCAA 6815 0.0 41.50478 1 TATCAAC 8670 0.0 32.406082 2 ATCAACG 8685 0.0 32.241463 3 TCAACGC 8705 0.0 32.11277 4 CAACGCA 8825 0.0 31.67611 5 AACGCAG 9200 0.0 30.482851 6 ACGCAGA 10385 0.0 26.678694 7 CGCAGAG 10445 0.0 26.252453 8 GTACATG 8080 0.0 24.593023 1 GTGGTAT 2150 0.0 23.216558 1 TGGTATC 1990 0.0 23.17271 2 TACATGG 8275 0.0 23.152355 2 GCAGAGT 11500 0.0 22.89362 9 ACATGGG 8480 0.0 21.864422 3 GAGTACT 5865 0.0 21.55342 12-13 CAGAGTA 10680 0.0 20.446135 10-11 CATGGGG 4820 0.0 20.41703 4 GTACTTT 6250 0.0 20.187967 14-15 AGAGTAC 10010 0.0 19.939404 10-11 >>END_MODULE