FastQCFastQC Report
Thu 26 May 2016
SRR936792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956094
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67790.3465579874995782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53990.2760092306402453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.18000157456645743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.049.084181
GTATCAA66850.043.3533171
TATCAAC89000.032.023322
ATCAACG89550.031.8258233
TCAACGC90600.031.4569784
CAACGCA92000.031.0299195
AACGCAG95650.029.8481046
ACGCAGA108900.025.91117
CGCAGAG109850.025.428858
GCAGAGT120250.022.8740449
GTACATG79750.022.4597321
TACATGG82250.021.4260962
GTGGTAT22500.020.9048271
AGAGTAC107400.020.7452210-11
AGTACTT67850.020.40846812-13
TGGTATC20450.020.2083572
GAGTACT67400.020.0513712-13
ACATGGG85450.019.733783
GTACTTT69200.019.49448414-15
GGACCGT2451.7561433E-719.3892576
Produced by FastQC (version 0.11.5)