##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956094 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13819070044691 33.0 31.0 34.0 31.0 34.0 2 32.27523166064616 33.0 31.0 34.0 31.0 34.0 3 32.40381137102818 34.0 31.0 34.0 31.0 34.0 4 36.06842053602741 37.0 35.0 37.0 35.0 37.0 5 35.89684186956251 37.0 35.0 37.0 35.0 37.0 6 35.98263529257797 37.0 35.0 37.0 35.0 37.0 7 35.93473524278485 37.0 35.0 37.0 35.0 37.0 8 35.88828144250736 37.0 35.0 37.0 35.0 37.0 9 37.585777575106306 39.0 37.0 39.0 35.0 39.0 10-11 37.494793195010054 39.0 37.0 39.0 35.0 39.0 12-13 37.38536900578397 39.0 37.0 39.0 34.0 39.0 14-15 38.599358466413165 40.0 38.0 41.0 34.0 41.0 16-17 38.58612648471903 40.0 38.0 41.0 34.0 41.0 18-19 38.681817182609834 40.0 38.0 41.0 34.0 41.0 20-21 38.6713601186855 40.0 38.0 41.0 34.0 41.0 22-23 38.576634353972764 40.0 38.0 41.0 34.0 41.0 24-25 38.486808404913056 40.0 38.0 41.0 34.0 41.0 26-27 38.28497761355027 40.0 38.0 41.0 34.0 41.0 28-29 38.12672882796021 40.0 38.0 41.0 33.0 41.0 30-31 38.158322146072734 40.0 38.0 41.0 34.0 41.0 32-33 38.05541553729013 40.0 37.0 41.0 33.0 41.0 34-35 37.95022018369261 40.0 37.0 41.0 33.0 41.0 36-37 37.81352685504889 40.0 37.0 41.0 33.0 41.0 38-39 37.65734826649435 40.0 37.0 41.0 33.0 41.0 40-41 37.32501786723951 40.0 36.5 41.0 32.0 41.0 42-43 37.14879013994215 39.5 36.0 41.0 31.0 41.0 44-45 36.94513172679841 39.0 36.0 41.0 31.0 41.0 46-47 36.601985640771865 39.0 36.0 41.0 30.5 41.0 48-49 36.25778822490126 39.0 35.0 40.0 30.0 41.0 50-51 35.97522435015904 39.0 35.0 40.0 29.0 41.0 52-53 35.69819650793878 38.5 35.0 40.0 28.5 41.0 54-55 35.28761910214949 38.0 34.0 40.0 28.0 41.0 56-57 35.07093242962762 38.0 34.0 40.0 27.5 41.0 58-59 34.72215113384122 37.5 34.0 40.0 26.5 41.0 60-61 34.313638557247245 37.0 33.5 40.0 26.0 41.0 62-63 33.90833773837045 36.5 33.0 39.0 26.0 40.0 64-65 33.5530720916275 36.0 33.0 39.0 25.5 40.0 66-67 33.19700305813524 35.5 32.0 39.0 24.5 40.0 68-69 32.7238345907712 35.0 32.0 38.0 23.5 40.0 70-71 32.269264156017044 35.0 31.0 37.5 22.0 39.5 72-73 31.84348502679319 35.0 31.0 37.0 22.0 39.0 74-75 31.358288763218944 34.0 31.0 36.0 20.5 39.0 76-77 29.952608872579745 33.0 29.0 35.0 19.5 37.0 78-79 30.64870808867059 34.0 30.0 35.0 20.0 37.0 80-81 30.53346004844348 34.0 30.0 35.0 18.5 37.0 82-83 30.198192162544338 34.0 30.0 35.0 18.0 36.5 84-85 29.76386743172874 34.0 29.5 35.0 14.0 36.0 86-87 29.385848532841468 34.0 29.0 35.0 8.5 35.5 88-89 29.003943062040985 34.0 29.0 35.0 6.0 35.0 90-91 28.549473593804798 33.0 29.0 35.0 2.0 35.0 92-93 28.03857866748735 33.0 27.5 35.0 2.0 35.0 94-95 27.44351754056809 33.0 26.5 34.0 2.0 35.0 96-97 26.63030125341625 32.0 25.0 34.0 2.0 35.0 98-99 25.723337937747367 32.0 24.0 34.0 2.0 35.0 100-101 22.50798453448556 28.0 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07596835158101811 1101 2 0.18974949043452938 1101 3 0.17430327144892743 1101 4 0.07791403287280474 1101 5 -0.025312657589395826 1101 6 0.0471313332856127 1101 7 0.07795615235784936 1101 8 0.12022583620790073 1101 9 0.09487278826224355 1101 10-11 0.19492059657758887 1101 12-13 0.16866737957905542 1101 14-15 0.3414150188151268 1101 16-17 0.24927364094737925 1101 18-19 0.12187411897347289 1101 20-21 0.056903161582262385 1101 22-23 0.08633821092138305 1101 24-25 0.05768799590828877 1101 26-27 0.07488184790330621 1101 28-29 0.1848160618540291 1101 30-31 0.18614561516302075 1101 32-33 0.2191342163533747 1101 34-35 0.004385136605030482 1101 36-37 0.16810631656262842 1101 38-39 0.10484821159674595 1101 40-41 0.23806883213470087 1101 42-43 0.11898280878727974 1101 44-45 0.10716656390177803 1101 46-47 -0.12346424325323113 1101 48-49 -0.09446878413054804 1101 50-51 0.012000893643872246 1101 52-53 0.006076704089437612 1101 54-55 -0.12772815839991125 1101 56-57 0.01752839395918926 1101 58-59 0.05045788590253153 1101 60-61 0.05762002561836965 1101 62-63 -0.015032981968808201 1101 64-65 -0.013029175993629849 1101 66-67 0.01719275489548977 1101 68-69 -0.2589769428225708 1101 70-71 -0.28152695339972666 1101 72-73 -0.04422046356456022 1101 74-75 -0.2030876948873832 1101 76-77 -0.15271228463094033 1101 78-79 -0.19531812903774082 1101 80-81 -0.11694781841075041 1101 82-83 0.06585332431351176 1101 84-85 -0.14517200434989164 1101 86-87 -0.5742363872547429 1101 88-89 -0.3130335080122286 1101 90-91 -0.09673225721300582 1101 92-93 -0.08765234666918431 1101 94-95 -0.2932770691558808 1101 96-97 -0.2655581690362574 1101 98-99 -0.1732559056948304 1101 100-101 0.09300452163919459 1102 1 -0.053571331034774516 1102 2 0.038332172340162174 1102 3 0.020236556514547033 1102 4 0.10499403314984335 1102 5 0.027577252060474677 1102 6 0.11603589586343332 1102 7 0.1645705982567165 1102 8 0.2233040485549509 1102 9 0.2403567999807521 1102 10-11 0.23174444232121516 1102 12-13 0.2522200752990571 1102 14-15 0.3608696788606949 1102 16-17 0.15300485263220764 1102 18-19 0.24036923173400027 1102 20-21 0.2246976732339121 1102 22-23 0.08063665351503602 1102 24-25 0.04314454936957901 1102 26-27 0.1614909662396471 1102 28-29 -0.019827068706945283 1102 30-31 0.08270219840538573 1102 32-33 -0.054619653454842876 1102 34-35 0.18202604930173294 1102 36-37 0.1068390812319393 1102 38-39 0.2720977136028395 1102 40-41 0.33708051601205824 1102 42-43 0.3006734681317411 1102 44-45 0.4274351156731626 1102 46-47 0.29350329750662496 1102 48-49 0.34931770634297976 1102 50-51 0.2780167452733835 1102 52-53 0.3422861220479092 1102 54-55 0.6799455028235855 1102 56-57 0.6351940052357179 1102 58-59 0.3756182055775952 1102 60-61 0.4169015185718692 1102 62-63 0.3536231563688119 1102 64-65 0.5625004769529696 1102 66-67 0.575949110011635 1102 68-69 0.4964895358876973 1102 70-71 0.37498164981460036 1102 72-73 0.2456301036343831 1102 74-75 0.1669432209579078 1102 76-77 0.4603396575053402 1102 78-79 0.10054086316856825 1102 80-81 0.4621177377787866 1102 82-83 0.3968579800703971 1102 84-85 0.3323848394102775 1102 86-87 0.16240541567765376 1102 88-89 0.3308969864761373 1102 90-91 0.4694286106682384 1102 92-93 0.48835678690399575 1102 94-95 0.3804400937465964 1102 96-97 0.5289902427907194 1102 98-99 0.29921684515478475 1102 100-101 0.6215485360960713 1103 1 0.05829347502927362 1103 2 0.18993340843067585 1103 3 0.15635894610477408 1103 4 0.10882332701696384 1103 5 0.0747921221000567 1103 6 0.044598086090182676 1103 7 0.09612832174382646 1103 8 0.10246650022686055 1103 9 0.054153343916006236 1103 10-11 0.10622998200220479 1103 12-13 0.12450804374087454 1103 14-15 0.15350002035610544 1103 16-17 0.08477390325371914 1103 18-19 0.15178043507459194 1103 20-21 0.17553928643365424 1103 22-23 0.17219211360769293 1103 24-25 0.19632207801999613 1103 26-27 0.17836018361328598 1103 28-29 -0.014558902656936823 1103 30-31 0.19176663124030568 1103 32-33 0.16365240655743918 1103 34-35 0.17235119284251255 1103 36-37 0.1695593580127408 1103 38-39 0.2294119420393841 1103 40-41 0.20194778409496905 1103 42-43 0.10111713963468105 1103 44-45 0.1701449069327694 1103 46-47 0.25055076874072313 1103 48-49 0.26187215483689386 1103 50-51 0.07558036282438962 1103 52-53 -0.06254622509213448 1103 54-55 0.23114209941741137 1103 56-57 0.05599252155756318 1103 58-59 -0.05659890476057683 1103 60-61 -0.1451212631860983 1103 62-63 0.18375424847972255 1103 64-65 0.08586540542508914 1103 66-67 0.017675941397982342 1103 68-69 0.02394589625703958 1103 70-71 -0.01929935291533269 1103 72-73 0.26399824243127057 1103 74-75 -0.0874496119342929 1103 76-77 0.21933087645872718 1103 78-79 0.12141374816503259 1103 80-81 0.1674422577313024 1103 82-83 0.21175850619412628 1103 84-85 -0.07984049305465035 1103 86-87 -0.28862673838098374 1103 88-89 0.04902585214599142 1103 90-91 0.01796918316845364 1103 92-93 -0.04268636078479915 1103 94-95 0.14780587214642438 1103 96-97 0.1453945404686614 1103 98-99 -0.06526818641725995 1103 100-101 0.6916975967873711 1104 1 0.033603255426598366 1104 2 0.13179625395008543 1104 3 0.08964261650474015 1104 4 0.043823702025257205 1104 5 -0.20541171573838568 1104 6 -0.026072497571263398 1104 7 0.17047308081277635 1104 8 0.11138227698859282 1104 9 0.13703524818998147 1104 10-11 0.17557734801283686 1104 12-13 0.1953053512175984 1104 14-15 0.24320064417424447 1104 16-17 0.11639027231115051 1104 18-19 0.20900814916537058 1104 20-21 0.19880818839126846 1104 22-23 0.21211247054311855 1104 24-25 0.21318739642015316 1104 26-27 0.19006026104253237 1104 28-29 0.2353862954072241 1104 30-31 0.23079928739684163 1104 32-33 0.1804806770373304 1104 34-35 0.22807198957027452 1104 36-37 0.23149614357213721 1104 38-39 0.24454162425337955 1104 40-41 0.40406900851333205 1104 42-43 0.2858555523314834 1104 44-45 0.3747318561370747 1104 46-47 0.30721026375130833 1104 48-49 0.47089362234034127 1104 50-51 0.5476146179293053 1104 52-53 0.39450237272107813 1104 54-55 0.6450789654133615 1104 56-57 0.5844843650367082 1104 58-59 0.29367414475247244 1104 60-61 0.4201059936482068 1104 62-63 0.45227947675469693 1104 64-65 0.4507409515875196 1104 66-67 0.17230182262216687 1104 68-69 0.2101378736884314 1104 70-71 0.3713393532299918 1104 72-73 0.4231372668807296 1104 74-75 0.24950122284864662 1104 76-77 0.3642787358135884 1104 78-79 0.5488649527025231 1104 80-81 0.3836845369990698 1104 82-83 0.5417197843238242 1104 84-85 0.4071332226687794 1104 86-87 0.4302996581806795 1104 88-89 0.39914101773479516 1104 90-91 0.5098012490220825 1104 92-93 0.36015895572400325 1104 94-95 0.3597345597007511 1104 96-97 0.7527357715610883 1104 98-99 0.6820651785592879 1104 100-101 1.4036627887716904 1105 1 -0.06579433437473625 1105 2 0.038330531081093966 1105 3 0.034915430501406775 1105 4 -0.025710185524225437 1105 5 0.025693998464973333 1105 6 0.0516401846557315 1105 7 0.09084633525042562 1105 8 -0.020838161072127548 1105 9 0.06571151709606937 1105 10-11 0.0549190203124752 1105 12-13 0.06831107034883388 1105 14-15 0.08365929042027176 1105 16-17 -0.1150628110081584 1105 18-19 0.0069141716867093805 1105 20-21 0.09810508205968915 1105 22-23 0.06272896704292918 1105 24-25 -0.0336305406945101 1105 26-27 -0.12709471523390903 1105 28-29 -0.15865998762236444 1105 30-31 0.029771684755125705 1105 32-33 0.010788167509318214 1105 34-35 -0.14798938765157743 1105 36-37 -0.03334723814430873 1105 38-39 -0.10859962090125208 1105 40-41 -0.04963996571571272 1105 42-43 -0.012145494610763308 1105 44-45 -0.043410834839228585 1105 46-47 0.009078116580603535 1105 48-49 -0.06697853727093417 1105 50-51 -0.05368262348014241 1105 52-53 -0.027083698298056902 1105 54-55 0.19738935124784263 1105 56-57 0.06910314695752362 1105 58-59 -0.06792594748257841 1105 60-61 0.04134013958600491 1105 62-63 -0.008960787193764475 1105 64-65 0.06291179471075026 1105 66-67 -0.037306336460986245 1105 68-69 0.060987096338458 1105 70-71 -0.059968961964713685 1105 72-73 -0.08447277595464442 1105 74-75 -0.03120844779224896 1105 76-77 -0.13654942711889007 1105 78-79 0.007732860389367602 1105 80-81 -0.08213261258443083 1105 82-83 -0.1041584151958439 1105 84-85 -0.27245614538162855 1105 86-87 -0.15855282430906925 1105 88-89 0.15625571555806417 1105 90-91 0.07952964839986976 1105 92-93 0.1529768946982415 1105 94-95 0.35697838660010106 1105 96-97 0.35460446120035627 1105 98-99 0.3403816284766741 1105 100-101 0.5923470724577058 1106 1 -0.1216198808456177 1106 2 -0.02088125767244975 1106 3 -0.03961527198482884 1106 4 0.05613947241003103 1106 5 -0.04293294129808345 1106 6 -0.002955802329090318 1106 7 0.01914184668042651 1106 8 0.10900707503666496 1106 9 0.1668858037638543 1106 10-11 0.16023198960998286 1106 12-13 0.15109828042726292 1106 14-15 0.2408494497728455 1106 16-17 0.1847395625943804 1106 18-19 0.1572802237474562 1106 20-21 0.06335099395689525 1106 22-23 0.15287945511234113 1106 24-25 0.22733433499379174 1106 26-27 0.17446648116189323 1106 28-29 0.2425205563455961 1106 30-31 0.2187927301414092 1106 32-33 0.10400003969353833 1106 34-35 0.021936463341248214 1106 36-37 0.1167487071251756 1106 38-39 0.10028313815097079 1106 40-41 0.152151186461829 1106 42-43 0.13026179305818175 1106 44-45 -0.18084304742057355 1106 46-47 -0.1655787747926638 1106 48-49 -0.0928034580117938 1106 50-51 -0.0888981874714645 1106 52-53 -0.09168823692645134 1106 54-55 0.04485723044023615 1106 56-57 -0.16816481149400175 1106 58-59 -0.08634848210630963 1106 60-61 -0.18197143224780632 1106 62-63 -0.16701445397166736 1106 64-65 -0.1584261203568218 1106 66-67 0.06772349000322464 1106 68-69 -0.18902958979536777 1106 70-71 -0.13727008410720742 1106 72-73 -0.07381346509197328 1106 74-75 -0.30038811573818336 1106 76-77 -0.09649488431895392 1106 78-79 -0.21130497541907545 1106 80-81 -0.09925258588661734 1106 82-83 -0.09821085137138397 1106 84-85 -0.1750565750495774 1106 86-87 -0.1598386994967882 1106 88-89 0.08504920379434111 1106 90-91 -0.15485943052554063 1106 92-93 -0.02813648758107945 1106 94-95 0.2167434249782083 1106 96-97 0.11432152694372277 1106 98-99 0.18332912972813276 1106 100-101 0.7743714133554178 1107 1 0.2632778603021251 1107 2 0.360750305209109 1107 3 0.2952595753900198 1107 4 0.20098837350893461 1107 5 0.14860399398885704 1107 6 0.17769895957567172 1107 7 0.2611748137133958 1107 8 0.12691000543959774 1107 9 0.2823162066942544 1107 10-11 0.16439484064783727 1107 12-13 0.28556867114765083 1107 14-15 0.27568232523071856 1107 16-17 0.3423097701645901 1107 18-19 0.3007822078744411 1107 20-21 0.2591690647749729 1107 22-23 0.2079885820964691 1107 24-25 0.3244993227837867 1107 26-27 0.4385919390373445 1107 28-29 0.376964237664275 1107 30-31 0.22189102073969735 1107 32-33 0.31126881258730776 1107 34-35 0.29659687237666077 1107 36-37 0.3452896106660788 1107 38-39 0.34539531763814324 1107 40-41 0.47725420843985233 1107 42-43 0.4412393571607467 1107 44-45 0.3709922700397428 1107 46-47 0.1430292838153946 1107 48-49 0.28030017446683786 1107 50-51 0.122411336338061 1107 52-53 0.14644897308575366 1107 54-55 0.41842636963436775 1107 56-57 0.15326757556338322 1107 58-59 0.3192948329120071 1107 60-61 0.2606224322210622 1107 62-63 0.193566284734068 1107 64-65 0.3138991238434201 1107 66-67 0.315193728098464 1107 68-69 0.1908238900581125 1107 70-71 0.09377387193674025 1107 72-73 0.23785091219740195 1107 74-75 0.27409295874289086 1107 76-77 0.08498405340997905 1107 78-79 0.43466953618043647 1107 80-81 0.4300705215614897 1107 82-83 0.5421890265284937 1107 84-85 0.4238254457074433 1107 86-87 0.7110327901246976 1107 88-89 0.5617128179579574 1107 90-91 0.6811181335770193 1107 92-93 0.7874579873883931 1107 94-95 1.0417357937205765 1107 96-97 0.8469184439156443 1107 98-99 0.9265106010346038 1107 100-101 1.4217462607302664 1108 1 -0.04068371325691089 1108 2 0.04227701957481145 1108 3 0.0126200957166418 1108 4 -0.010873988247496413 1108 5 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-1.2705154954944788 2316 64-65 -1.3722347851360936 2316 66-67 -1.3274029313013855 2316 68-69 -1.294150889799969 2316 70-71 -1.2302822428790208 2316 72-73 -1.3710672398928736 2316 74-75 -1.0113497117424188 2316 76-77 -1.2012658931575153 2316 78-79 -1.2313537140831627 2316 80-81 -1.3085238651758502 2316 82-83 -1.2951187945652336 2316 84-85 -1.2302997217144025 2316 86-87 -1.2868690935491784 2316 88-89 -2.035844693227247 2316 90-91 -1.9357561183679195 2316 92-93 -1.8185872770668823 2316 94-95 -2.012212779275348 2316 96-97 -1.8088729270911692 2316 98-99 -1.9337197643012374 2316 100-101 -2.9740572394549645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 16.0 5 50.0 6 73.0 7 207.0 8 490.0 9 989.0 10 1580.0 11 2621.0 12 3854.0 13 5415.0 14 6888.0 15 9482.0 16 12434.0 17 16693.0 18 15554.0 19 10746.0 20 9995.0 21 10635.0 22 11190.0 23 12632.0 24 14741.0 25 17421.0 26 21758.0 27 26736.0 28 33412.0 29 42172.0 30 52403.0 31 65521.0 32 83501.0 33 108919.0 34 149508.0 35 212795.0 36 324579.0 37 432756.0 38 228527.0 39 9789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.292340275615295 18.827098471798035 14.812979720273642 23.067581532313035 2 16.052405961645917 20.230752281270753 39.11572224860777 24.601119508475556 3 19.8161228860241 24.003202308279427 29.31308340264681 26.867591403049655 4 13.922285943313561 16.679004178735788 35.61659102272181 33.78211885522884 5 14.76042265243647 36.82166103961702 33.20560850867165 15.212307799274857 6 33.100709884085326 35.31890594214798 16.846480792845334 14.733903380921367 7 29.296504762050823 32.18778277073141 20.078319504726267 18.4373929624915 8 27.094096704964077 34.62671016832524 18.96764674908261 19.31154637762807 9 26.699817799980778 16.584598270428998 19.67744870895643 37.03813522063379 10-11 25.58253846696529 26.329230599347476 26.54409757404296 21.54413335964427 12-13 26.396398699359906 23.396005030777726 27.415386817726333 22.792209452136035 14-15 23.445984979780473 24.773188550277858 25.44828044559644 26.332546024345234 16-17 23.051295080911245 28.03735403308839 25.918641946654912 22.99270893934545 18-19 22.96481511634357 26.56208343057281 28.29514005423598 22.177961398847646 20-21 23.656913622344362 25.677927153025475 28.462000140076572 22.20315908455359 22-23 23.44768230406138 25.527297489492906 28.39369875857528 22.631321447870437 24-25 23.253474799650732 25.73767281977666 28.389859535684565 22.61899284488805 26-27 23.155926794816807 26.016913323562147 28.233119011334338 22.59404087028671 28-29 22.926196722853526 25.992182371790285 28.466963721542747 22.614657183813442 30-31 23.152190833413638 25.760527295857788 28.359712866486188 22.727569004242383 32-33 23.06300991417057 25.860249712564205 28.33753985631533 22.739200516949893 34-35 23.295225076095523 25.942004832078624 28.17497523125167 22.587794860574185 36-37 23.400355817306217 25.8085385928857 28.081475005879163 22.70963058392892 38-39 23.26074174812876 25.94666448525502 28.175180988997507 22.617412777618718 40-41 23.35480397928205 25.78187565293164 28.077485523992742 22.785834843793573 42-43 23.218286033288788 25.97137458629289 27.862439126136064 22.947900254282256 44-45 23.316622142970527 26.109000449625004 27.70306038957105 22.87131701783342 46-47 23.523974746554284 26.225998350279983 27.405136625905925 22.844890277259807 48-49 23.53487734846624 26.450437748151224 27.127604781067134 22.887080122315403 50-51 23.439038087503896 26.514912746864045 27.02325565939517 23.022793506236894 52-53 23.623636468205024 26.56289659843949 26.818508885722135 22.99495804763335 54-55 23.495931177131567 26.482341850647263 26.86200151935439 23.159725452866784 56-57 23.411191112056308 26.826758305352318 26.81098703125831 22.95106355133307 58-59 23.49873536144124 26.557895396051055 26.912684343915227 23.030684898592472 60-61 23.507349090019545 26.369073317592495 27.166723285627704 22.956854306760253 62-63 23.40057451256152 26.512568676438185 27.15832018211813 22.928536628882163 64-65 23.3764507557797 26.4756140110961 27.087752751978755 23.060182481145446 66-67 23.36289736735421 26.679252980303087 26.91681837213261 23.041031280210092 68-69 23.30595133507371 26.55812703962317 27.09457972419988 23.041341901103245 70-71 23.297748667492122 26.467956930319016 26.966580201483985 23.267714200704877 72-73 23.33677472852044 26.635427421026627 26.769700624179965 23.25809722627297 74-75 23.31408013848395 26.82336599628126 26.56404090102498 23.298512964209813 76-77 23.442545932120815 26.751284320326285 26.566502717406586 23.239667030146315 78-79 23.411484254023467 26.75361576453399 26.556104099664278 23.278795881778265 80-81 23.329442847692345 26.912293832378857 26.527437381585578 23.230825938343216 82-83 23.43040876687454 26.82242973931932 26.467640428702044 23.279521065104095 84-85 23.416997052726295 26.784455367846853 26.34681206209469 23.451735517332168 86-87 23.412096100118347 26.723992055559957 26.54401521406278 23.31989663025891 88-89 23.3862505543719 26.636972356173246 26.499373096443456 23.4774039930114 90-91 23.399686870946095 26.717218902770234 26.519858133367414 23.363236092916253 92-93 23.3555749365828 26.663135820671197 26.485767043915065 23.495522198830933 94-95 23.344558083609478 26.59475976103398 26.408674634245592 23.65200752111095 96-97 23.362119537086148 26.608625636634354 26.410832848735037 23.61842197754446 98-99 23.108908178043933 26.6047792548256 26.420763169585687 23.865549397544783 100-101 23.042019577695385 26.809658738709725 26.226136568420326 23.922185115174567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 278.0 1 217.5 2 186.5 3 230.0 4 214.5 5 206.5 6 348.5 7 751.0 8 1298.0 9 1671.0 10 1688.0 11 1618.5 12 1655.0 13 1791.5 14 1989.5 15 2165.5 16 2381.0 17 2558.5 18 2715.5 19 2896.0 20 3163.5 21 3579.0 22 4177.0 23 4998.0 24 6028.0 25 7356.5 26 9102.5 27 11141.5 28 13088.5 29 15256.5 30 17752.0 31 20279.5 32 23102.5 33 25933.5 34 29163.5 35 32223.5 36 35802.5 37 39744.0 38 43344.0 39 46021.5 40 48533.5 41 52194.0 42 55790.5 43 59571.0 44 63598.0 45 67486.0 46 70757.5 47 74464.0 48 78602.0 49 79964.0 50 79074.5 51 78161.5 52 77825.0 53 77526.0 54 76545.5 55 73609.0 56 68501.0 57 62402.0 58 56639.5 59 50829.0 60 43521.5 61 35927.5 62 28808.5 63 22305.5 64 16508.5 65 11839.0 66 8325.5 67 6336.5 68 4589.0 69 2937.0 70 1992.0 71 1215.0 72 715.5 73 435.0 74 262.0 75 142.5 76 85.5 77 42.5 78 18.5 79 13.0 80 7.5 81 3.5 82 4.5 83 3.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020295548168953025 2 0.0029139703920159255 3 2.5561143789613384E-4 4 0.0 5 0.0033740709802289664 6 0.0 7 2.0448915031690706E-4 8 0.0 9 1.0224457515845353E-4 10-11 0.0 12-13 0.006850386535616386 14-15 0.003271826405070513 16-17 0.0 18-19 6.390285947403346E-4 20-21 0.001073568039163762 22-23 0.0017892800652729369 24-25 5.112228757922677E-4 26-27 1.5336686273768028E-4 28-29 1.0224457515845353E-4 30-31 2.5561143789613383E-5 32-33 2.5561143789613384E-4 34-35 0.0 36-37 0.0017381577776937102 38-39 0.008895278038785457 40-41 6.390285947403346E-4 42-43 0.0 44-45 9.96884607794922E-4 46-47 2.3005029410652045E-4 48-49 1.789280065272937E-4 50-51 0.0016614743463248698 52-53 3.32294869264974E-4 54-55 0.0 56-57 3.0673372547536056E-4 58-59 7.668343136884014E-5 60-61 0.0018148412090625502 62-63 5.1122287579226765E-5 64-65 7.668343136884014E-5 66-67 5.1122287579226765E-5 68-69 1.789280065272937E-4 70-71 4.089783006338141E-4 72-73 3.834171568442007E-4 74-75 0.002811725816857472 76-77 5.1122287579226765E-5 78-79 3.578560130545874E-4 80-81 0.0020448915031690707 82-83 1.789280065272937E-4 84-85 0.0025049920913821117 86-87 4.601005882130409E-4 88-89 0.002632797810330178 90-91 0.0016103520587456431 92-93 0.0 94-95 0.0 96-97 3.32294869264974E-4 98-99 7.668343136884014E-4 100-101 0.002121574934537911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1956094.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.43521456865205 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54011424113945 44.72637998790124 2 13.23808444376133 15.471406098982563 3 4.262769717222059 7.472875892478697 4 1.917208214121201 4.4812989345981835 5 1.1042405322530813 3.2263266218805673 6 0.6491744051404951 2.276078739411713 7 0.45886188271754624 1.8769584801782824 8 0.31489773684695926 1.4720893455867996 9 0.2353305470368596 1.2376431909591439 >10 1.199994198896986 12.416075280542593 >50 0.05872844665359998 2.345394717481258 >100 0.01971968004299581 1.9300791715695098 >500 5.255725004243216E-4 0.18898081541656586 >1k 1.7519083347476987E-4 0.25506267692869167 >5k 1.7519083347476987E-4 0.6233500460842076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6779 0.3465579874995782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5399 0.2760092306402453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3521 0.18000157456645743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0224457515845353E-4 1.0224457515845353E-4 0.0 0.0 0.0 7 1.0224457515845353E-4 1.0224457515845353E-4 0.0 5.1122287579226765E-5 0.0 8 1.0224457515845353E-4 1.533668627376803E-4 0.0 5.1122287579226765E-5 0.0 9 1.0224457515845353E-4 1.533668627376803E-4 0.0 5.1122287579226765E-5 0.0 10-11 1.0224457515845353E-4 1.533668627376803E-4 0.0 5.1122287579226765E-5 0.0 12-13 1.0224457515845353E-4 1.533668627376803E-4 0.0 1.0224457515845353E-4 0.0 14-15 1.0224457515845353E-4 1.533668627376803E-4 0.0 1.0224457515845353E-4 0.0 16-17 1.2780571894806692E-4 1.789280065272937E-4 0.0 1.2780571894806692E-4 0.0 18-19 1.533668627376803E-4 2.5561143789613384E-4 0.0 1.533668627376803E-4 0.0 20-21 1.533668627376803E-4 3.067337254753606E-4 0.0 2.0448915031690706E-4 0.0 22-23 1.789280065272937E-4 3.067337254753606E-4 0.0 2.5561143789613384E-4 0.0 24-25 2.5561143789613384E-4 3.067337254753606E-4 0.0 3.067337254753606E-4 0.0 26-27 2.5561143789613384E-4 3.067337254753606E-4 0.0 5.112228757922677E-4 0.0 28-29 2.811725816857472E-4 3.067337254753606E-4 0.0 7.412731698987882E-4 0.0 30-31 3.067337254753606E-4 3.067337254753606E-4 0.0 0.0015081074835871897 0.0 32-33 3.067337254753606E-4 3.067337254753606E-4 0.0 0.0025049920913821112 0.0 34-35 3.32294869264974E-4 3.067337254753606E-4 0.0 0.00355299898675626 0.0 36-37 4.089783006338141E-4 3.067337254753606E-4 0.0 0.004473200163182342 5.1122287579226765E-5 38-39 4.089783006338141E-4 3.067337254753606E-4 0.0 0.005904624215400691 5.1122287579226765E-5 40-41 4.089783006338141E-4 3.067337254753606E-4 0.0 0.008971961470154298 5.1122287579226765E-5 42-43 4.089783006338141E-4 3.067337254753606E-4 0.0 0.012115982156276742 5.1122287579226765E-5 44-45 4.345394444234275E-4 3.067337254753606E-4 0.0 0.01919641898599965 5.1122287579226765E-5 46-47 4.601005882130409E-4 3.067337254753606E-4 0.0 0.034430860684609224 5.1122287579226765E-5 48-49 4.601005882130409E-4 3.067337254753606E-4 0.0 0.04685357656636133 5.1122287579226765E-5 50-51 4.601005882130409E-4 3.067337254753606E-4 0.0 0.060349860487277196 5.1122287579226765E-5 52-53 4.601005882130409E-4 3.067337254753606E-4 0.0 0.07729689881979088 7.668343136884014E-5 54-55 4.601005882130409E-4 3.067337254753606E-4 0.0 0.09690229610642434 1.0224457515845353E-4 56-57 4.601005882130409E-4 3.067337254753606E-4 0.0 0.12031630381771019 1.0224457515845353E-4 58-59 4.601005882130409E-4 3.067337254753606E-4 0.0 0.15727771773749114 1.0224457515845353E-4 60-61 4.601005882130409E-4 3.067337254753606E-4 0.0 0.19965809414067015 1.0224457515845353E-4 62-63 4.601005882130409E-4 3.067337254753606E-4 0.0 0.25226292805969447 1.0224457515845353E-4 64-65 4.601005882130409E-4 3.067337254753606E-4 0.0 0.29615141194646066 1.2780571894806692E-4 66-67 4.601005882130409E-4 3.067337254753606E-4 0.0 0.362048040636084 1.533668627376803E-4 68-69 4.601005882130409E-4 3.067337254753606E-4 0.0 0.44455941278895594 1.533668627376803E-4 70-71 4.856617320026543E-4 3.067337254753606E-4 0.0 0.508283344256462 1.533668627376803E-4 72-73 5.112228757922677E-4 3.067337254753606E-4 0.0 0.6070516038595282 3.32294869264974E-4 74-75 5.112228757922677E-4 3.067337254753606E-4 0.0 0.6916078675155692 3.5785601305458734E-4 76-77 5.112228757922677E-4 3.067337254753606E-4 0.0 0.7699783343745239 3.5785601305458734E-4 78-79 5.623451633714944E-4 3.067337254753606E-4 0.0 0.8677752705135848 3.5785601305458734E-4 80-81 5.623451633714944E-4 3.067337254753606E-4 0.0 0.9633995094305284 3.5785601305458734E-4 82-83 6.390285947403346E-4 3.067337254753606E-4 0.0 1.0901316603394315 4.089783006338141E-4 84-85 6.64589738529948E-4 3.067337254753606E-4 0.0 1.2586051590567733 4.089783006338141E-4 86-87 6.64589738529948E-4 3.067337254753606E-4 0.0 1.4748524355169026 4.089783006338141E-4 88-89 6.64589738529948E-4 3.067337254753606E-4 0.0 1.736956403935598 4.089783006338141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 49.08418 1 GTATCAA 6685 0.0 43.353317 1 TATCAAC 8900 0.0 32.02332 2 ATCAACG 8955 0.0 31.825823 3 TCAACGC 9060 0.0 31.456978 4 CAACGCA 9200 0.0 31.029919 5 AACGCAG 9565 0.0 29.848104 6 ACGCAGA 10890 0.0 25.9111 7 CGCAGAG 10985 0.0 25.42885 8 GCAGAGT 12025 0.0 22.874044 9 GTACATG 7975 0.0 22.459732 1 TACATGG 8225 0.0 21.426096 2 GTGGTAT 2250 0.0 20.904827 1 AGAGTAC 10740 0.0 20.74522 10-11 AGTACTT 6785 0.0 20.408468 12-13 TGGTATC 2045 0.0 20.208357 2 GAGTACT 6740 0.0 20.05137 12-13 ACATGGG 8545 0.0 19.73378 3 GTACTTT 6920 0.0 19.494484 14-15 GGACCGT 245 1.7561433E-7 19.389257 6 >>END_MODULE