FastQCFastQC Report
Thu 26 May 2016
SRR936791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661425
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105420.39610359112129784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73590.27650600712024576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.18422461651183106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.047.441011
GTATCAA96250.043.0137521
ATCAACG121000.033.545783
TATCAAC121950.033.4812162
TCAACGC122000.033.271444
CAACGCA124350.032.715425
AACGCAG128350.031.8069346
CGCAGAG143200.028.1439828
ACGCAGA143800.027.9924457
GTACATG98600.025.5498811
GCAGAGT153550.025.443169
TACATGG101750.023.82462
ACATGGG98950.023.728843
CGACCGT1050.00219019722.6310776
GAGTACT86150.022.00925612-13
AGAGTAC134450.021.43749810-11
GTACTTT90850.020.8967914-15
CATGGGG52050.020.8203334
AGTACTT90900.020.51934612-13
CAGAGTA148000.020.1812310-11
Produced by FastQC (version 0.11.5)