##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936791_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2661425 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530755892050312 33.0 31.0 34.0 30.0 34.0 2 31.642495279784328 33.0 31.0 34.0 30.0 34.0 3 31.648730285467373 33.0 31.0 34.0 30.0 34.0 4 35.227281625445016 37.0 35.0 37.0 33.0 37.0 5 35.25074311693923 37.0 35.0 37.0 33.0 37.0 6 35.287695501470075 37.0 35.0 37.0 33.0 37.0 7 35.215930939252466 37.0 35.0 37.0 32.0 37.0 8 35.17886696036898 37.0 35.0 37.0 32.0 37.0 9 36.773379674422536 39.0 37.0 39.0 33.0 39.0 10-11 36.63114853884667 39.0 37.0 39.0 32.0 39.0 12-13 36.50734343443831 39.0 37.0 39.0 32.0 39.0 14-15 37.625888386860424 40.0 37.0 41.0 32.0 41.0 16-17 37.5494855199752 40.0 37.0 41.0 32.0 41.0 18-19 37.48975755469344 40.0 37.0 41.0 32.0 41.0 20-21 37.41895225302235 40.0 37.0 41.0 32.0 41.0 22-23 37.27680471927633 40.0 37.0 41.0 31.5 41.0 24-25 37.10303859774369 39.5 36.5 41.0 31.0 41.0 26-27 37.06169176287139 39.5 36.5 41.0 31.0 41.0 28-29 37.13844312727204 40.0 37.0 41.0 31.0 41.0 30-31 37.01403815625088 39.5 36.5 41.0 31.0 41.0 32-33 36.84351540997774 39.0 36.0 41.0 30.0 41.0 34-35 36.65801516105094 39.0 36.0 41.0 30.0 41.0 36-37 36.51253088101299 39.0 35.5 41.0 30.0 41.0 38-39 36.3601140366533 39.0 35.0 41.0 30.0 41.0 40-41 36.15937589825 39.0 35.0 41.0 29.5 41.0 42-43 35.96704772819072 39.0 35.0 40.0 29.0 41.0 44-45 35.735008688954224 39.0 35.0 40.0 27.5 41.0 46-47 35.42190818828259 38.5 35.0 40.0 26.5 41.0 48-49 35.10619912265046 38.0 34.5 40.0 25.5 41.0 50-51 34.47633110082005 37.5 33.5 39.5 24.0 40.5 52-53 34.78741181885644 38.0 34.5 40.0 26.0 40.5 54-55 35.09710249208601 39.0 35.0 40.0 26.0 41.0 56-57 34.98803366617508 38.5 34.5 40.0 26.0 41.0 58-59 34.74496782738571 38.0 34.0 40.0 25.0 41.0 60-61 34.46286049766573 38.0 34.0 40.0 24.0 41.0 62-63 34.14219299811191 38.0 34.0 40.0 23.0 41.0 64-65 33.832483913692855 37.0 33.5 40.0 23.0 41.0 66-67 33.45780005072471 36.5 33.0 39.5 22.0 41.0 68-69 33.02209680904027 36.0 32.5 39.0 21.0 41.0 70-71 32.672524681326735 35.5 32.0 39.0 20.5 40.5 72-73 32.28711630047813 35.0 32.0 38.0 20.0 40.0 74-75 31.85449561794903 35.0 31.5 37.0 18.5 39.5 76-77 31.40695078764196 35.0 31.0 37.0 17.0 39.0 78-79 30.975801497318166 35.0 31.0 36.0 12.5 39.0 80-81 30.55099880703007 35.0 30.5 36.0 8.0 37.5 82-83 30.13618907164395 34.0 30.0 35.5 7.0 37.0 84-85 29.779181077806065 34.0 30.0 35.0 4.0 37.0 86-87 29.439514921517606 34.0 29.5 35.0 2.0 36.0 88-89 29.069973040758242 34.0 29.0 35.0 2.0 36.0 90-91 28.725214311881793 34.0 29.0 35.0 2.0 36.0 92-93 28.359234244812463 34.0 29.0 35.0 2.0 35.0 94-95 27.94912950768855 34.0 28.5 35.0 2.0 35.0 96-97 27.4925598128822 34.0 27.0 35.0 2.0 35.0 98-99 26.86742459396752 34.0 26.0 35.0 2.0 35.0 100-101 24.681559690767166 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4347456549634252 1101 2 0.36520386424487583 1101 3 0.4816397945943258 1101 4 0.36925162173437087 1101 5 0.3457140937184846 1101 6 0.35744631816955774 1101 7 0.45809304385208804 1101 8 0.35199720494895814 1101 9 0.4411901236244091 1101 10-11 0.38957903850457143 1101 12-13 0.5165443653961788 1101 14-15 0.7414635500100886 1101 16-17 0.7403847468381741 1101 18-19 0.7998033061460958 1101 20-21 0.69441606843899 1101 22-23 0.6526052170372907 1101 24-25 0.7640146979955205 1101 26-27 0.8901548051620409 1101 28-29 0.7500021238293755 1101 30-31 0.6761254562679184 1101 32-33 0.7488431655685659 1101 34-35 0.6878458778011947 1101 36-37 0.5805080015743513 1101 38-39 0.8383342989672897 1101 40-41 0.6710411863454979 1101 42-43 0.7129998364244159 1101 44-45 0.6787660953570338 1101 46-47 0.8865185435621967 1101 48-49 0.8634952851958246 1101 50-51 0.9671095105071288 1101 52-53 0.9361228196597082 1101 54-55 0.9007133226773973 1101 56-57 0.8366257224781393 1101 58-59 0.8565312308081445 1101 60-61 0.9960189513888054 1101 62-63 0.9542834529174655 1101 64-65 0.884516777317387 1101 66-67 0.8369146314268505 1101 68-69 0.8020100637720518 1101 70-71 0.7688911400499734 1101 72-73 0.7751589445396405 1101 74-75 0.6469853563252741 1101 76-77 0.7584582675936495 1101 78-79 0.7225759719350258 1101 80-81 0.7295015102027627 1101 82-83 0.5635219925345396 1101 84-85 0.8377158441277182 1101 86-87 0.9855814013975497 1101 88-89 1.0168222156994062 1101 90-91 0.9357295559442989 1101 92-93 0.8480354002653918 1101 94-95 1.0145143819926261 1101 96-97 1.1269728402726749 1101 98-99 1.2430566503324734 1101 100-101 1.4238385102832325 1102 1 0.3725407275532966 1102 2 0.31360812242097325 1102 3 0.3016408150956629 1102 4 0.27897592524220727 1102 5 0.3019098795850752 1102 6 0.2993362866414344 1102 7 0.3008149894647403 1102 8 0.33853652379117705 1102 9 0.36529758778601007 1102 10-11 0.45519646866736707 1102 12-13 0.2974873086294494 1102 14-15 0.5508576676255572 1102 16-17 0.5361848467398858 1102 18-19 0.539138261539442 1102 20-21 0.44776760966455953 1102 22-23 0.5229801975671933 1102 24-25 0.5028718976324313 1102 26-27 0.4064761126928005 1102 28-29 0.6155910873759964 1102 30-31 0.5868976535147112 1102 32-33 0.6250041201132888 1102 34-35 0.5365843501644036 1102 36-37 0.5949177489734083 1102 38-39 0.5686919309745022 1102 40-41 0.5972050949730274 1102 42-43 0.5901407408034416 1102 44-45 0.564690427868598 1102 46-47 0.45500707366429793 1102 48-49 0.5332387633866418 1102 50-51 0.33167099034148606 1102 52-53 0.4986187242267164 1102 54-55 0.7624417028195154 1102 56-57 0.7368343881457875 1102 58-59 0.5513704481373338 1102 60-61 0.6968419320046522 1102 62-63 0.8129136715733054 1102 64-65 0.9730588300289682 1102 66-67 0.7562564886318484 1102 68-69 0.6287591493491504 1102 70-71 0.6497031637041175 1102 72-73 0.6555479972458471 1102 74-75 0.7270203219236322 1102 76-77 0.7086524314002212 1102 78-79 0.8448024910049732 1102 80-81 0.7674527463521414 1102 82-83 0.7186669305793671 1102 84-85 0.8260219730438898 1102 86-87 0.9476406388145016 1102 88-89 0.9391001626655608 1102 90-91 0.658563273308399 1102 92-93 0.7998528325766117 1102 94-95 1.0461820154500288 1102 96-97 1.017943820437356 1102 98-99 1.103236151146188 1102 100-101 1.4628959161688364 1103 1 0.3905480372957797 1103 2 0.4436885505331105 1103 3 0.42956916286346924 1103 4 0.4756022382206666 1103 5 0.38680499839457383 1103 6 0.273425448743879 1103 7 0.42203052214389203 1103 8 0.40622704311800817 1103 9 0.5270094079956706 1103 10-11 0.4481323042086345 1103 12-13 0.2985460693243951 1103 14-15 0.471276249870634 1103 16-17 0.6938616472090473 1103 18-19 0.610639429213208 1103 20-21 0.6155199162129534 1103 22-23 0.5366524530102339 1103 24-25 0.8037761902734744 1103 26-27 0.8047143914474262 1103 28-29 0.6619618735398447 1103 30-31 0.8049748665968437 1103 32-33 0.8466270295583556 1103 34-35 0.7671800208699295 1103 36-37 0.7126781716399009 1103 38-39 0.8027042255843568 1103 40-41 0.7880236709346562 1103 42-43 0.9105449234841672 1103 44-45 0.8495187850615764 1103 46-47 0.8507010803038071 1103 48-49 0.9882474554872971 1103 50-51 0.8999856653302274 1103 52-53 0.9017827012293012 1103 54-55 0.8495444057486523 1103 56-57 0.9204756976159914 1103 58-59 0.9139226792843829 1103 60-61 0.8944447972561491 1103 62-63 0.9445709828866455 1103 64-65 0.9572695768165502 1103 66-67 0.969133364502369 1103 68-69 0.9224853900679832 1103 70-71 0.9354449768972657 1103 72-73 0.9824372200882721 1103 74-75 0.8904428103778059 1103 76-77 0.8344208445158721 1103 78-79 0.9486429857216407 1103 80-81 0.9741872916924414 1103 82-83 0.9142425989809624 1103 84-85 1.127254052131537 1103 86-87 1.145454261325991 1103 88-89 1.3120470465998366 1103 90-91 1.1681135880043314 1103 92-93 0.9522088631141621 1103 94-95 1.019989993332299 1103 96-97 0.8852589038501044 1103 98-99 1.1252698408905566 1103 100-101 1.4717257290268648 1104 1 0.4447642406575305 1104 2 0.5107384917111588 1104 3 0.38058603985602346 1104 4 0.536428365142747 1104 5 0.4551617003964097 1104 6 0.42415086702020943 1104 7 0.41740967833101195 1104 8 0.5484474262245342 1104 9 0.5402124563430633 1104 10-11 0.60272104661572 1104 12-13 0.5639324332351734 1104 14-15 0.6927508173153427 1104 16-17 0.7003419052382043 1104 18-19 0.5796849990354715 1104 20-21 0.6046171186397444 1104 22-23 0.7524607154868619 1104 24-25 0.6936463200072467 1104 26-27 0.7622701253149557 1104 28-29 0.6131595998258845 1104 30-31 0.7287938078436653 1104 32-33 0.7575950404943583 1104 34-35 0.8355132964574778 1104 36-37 0.8219217097555216 1104 38-39 0.7174830644846466 1104 40-41 0.742272211934953 1104 42-43 0.8078920822078999 1104 44-45 0.7676708963786467 1104 46-47 0.8164470035827591 1104 48-49 0.7521034905253359 1104 50-51 0.67626157153218 1104 52-53 0.7884509585298147 1104 54-55 0.8517750334820633 1104 56-57 0.8549551666277679 1104 58-59 0.842575401919305 1104 60-61 0.808742640031177 1104 62-63 0.7684271176674073 1104 64-65 0.8151138296149938 1104 66-67 0.6974649812110627 1104 68-69 0.8063657072078172 1104 70-71 0.6802668206571241 1104 72-73 0.6677593181920543 1104 74-75 0.6991331307671516 1104 76-77 0.6620271085684344 1104 78-79 0.7041065898669494 1104 80-81 0.8547184846520786 1104 82-83 0.7542707028036837 1104 84-85 0.6968763054664855 1104 86-87 0.7983767754094018 1104 88-89 0.8746644739622056 1104 90-91 0.5667122553366504 1104 92-93 0.6115685883702255 1104 94-95 0.4714637838337481 1104 96-97 0.5162704367329134 1104 98-99 0.5832984339775464 1104 100-101 0.9723077146523629 1105 1 0.1893958822532653 1105 2 0.2822114326465055 1105 3 0.26773066033016946 1105 4 0.1894766525482936 1105 5 0.18246001128645162 1105 6 0.24853756040992891 1105 7 0.28843243956892195 1105 8 0.13433583973031915 1105 9 0.29179642802765926 1105 10-11 0.29867888882750293 1105 12-13 0.24179335378794775 1105 14-15 0.2620539223268352 1105 16-17 0.47976751719815525 1105 18-19 0.37629442922608547 1105 20-21 0.4205997788713347 1105 22-23 0.38421838703370526 1105 24-25 0.4792816264979649 1105 26-27 0.3564721813085612 1105 28-29 0.2949722874965559 1105 30-31 0.11556661785705558 1105 32-33 0.2673126944623121 1105 34-35 0.2877627825904625 1105 36-37 0.30558293918688406 1105 38-39 0.23108856833051306 1105 40-41 0.3711174119998546 1105 42-43 0.36035624618288864 1105 44-45 0.27781928553937973 1105 46-47 0.28585140395298936 1105 48-49 0.36126756732850396 1105 50-51 0.1960991705037216 1105 52-53 0.2924880581191829 1105 54-55 0.3155753436744604 1105 56-57 0.44411400129757794 1105 58-59 0.5012118823063076 1105 60-61 0.39934982753467096 1105 62-63 0.3971239220024785 1105 64-65 0.5199101188677631 1105 66-67 0.517937614056116 1105 68-69 0.46987520381193093 1105 70-71 0.44314397671438144 1105 72-73 0.49390094381833194 1105 74-75 0.5595007632870335 1105 76-77 0.40743239182429036 1105 78-79 0.3938399570967839 1105 80-81 0.29813359657950755 1105 82-83 0.4278113303225197 1105 84-85 0.46597990723251925 1105 86-87 0.42315827107281834 1105 88-89 0.44141476513708966 1105 90-91 0.5411125138960102 1105 92-93 0.5355357140191686 1105 94-95 0.585357276559467 1105 96-97 0.687336028433787 1105 98-99 0.8170366202913719 1105 100-101 1.0196791920602806 1106 1 0.6134406408500865 1106 2 0.5792702019179146 1106 3 0.5007184309674493 1106 4 0.5139344187064694 1106 5 0.5515152149824871 1106 6 0.48043558731556146 1106 7 0.5388842980291528 1106 8 0.5426792270711971 1106 9 0.7239962596368699 1106 10-11 0.7593591226855594 1106 12-13 0.7213449413618562 1106 14-15 0.974100652615995 1106 16-17 0.9143693895679661 1106 18-19 0.9714364065708097 1106 20-21 0.9389660340062562 1106 22-23 0.9912009967367652 1106 24-25 1.0527605935141793 1106 26-27 1.1148435722888053 1106 28-29 1.063327953640048 1106 30-31 1.1531458046963081 1106 32-33 1.1362420109152467 1106 34-35 1.0616946042438116 1106 36-37 1.0359582174707427 1106 38-39 0.9986064128103251 1106 40-41 0.9314959887360956 1106 42-43 1.0414134628941554 1106 44-45 1.201552941781614 1106 46-47 1.05980455117097 1106 48-49 1.157676017565045 1106 50-51 0.9939738455471243 1106 52-53 1.0755213946255182 1106 54-55 1.2203674710729402 1106 56-57 1.4193248402925178 1106 58-59 1.3975030050419406 1106 60-61 1.4282824203027147 1106 62-63 1.3291136709732783 1106 64-65 1.2667144202858012 1106 66-67 1.3189129819221037 1106 68-69 1.3381072702296208 1106 70-71 1.3901385848147072 1106 72-73 1.3509667527398008 1106 74-75 1.3110307803105954 1106 76-77 1.3453389888028475 1106 78-79 1.4461927424442713 1106 80-81 1.5465414476340875 1106 82-83 1.5133747347681208 1106 84-85 1.5752311437832098 1106 86-87 1.6468944362050024 1106 88-89 1.6141609149133949 1106 90-91 1.5888168426904663 1106 92-93 1.5451221825425634 1106 94-95 1.6614475552750037 1106 96-97 1.7179895721301683 1106 98-99 1.9186690221624154 1106 100-101 2.0767935553814887 1107 1 0.24817743964299765 1107 2 0.2874069013256282 1107 3 0.3502470747483919 1107 4 0.26530458243883004 1107 5 0.20331298826577893 1107 6 0.2970070512848153 1107 7 0.3531746049883324 1107 8 0.3208784197812591 1107 9 0.44264843722618963 1107 10-11 0.4945332977274006 1107 12-13 0.48068332896141186 1107 14-15 0.44814520051509277 1107 16-17 0.45704692078183484 1107 18-19 0.4403423600514813 1107 20-21 0.49744052099462266 1107 22-23 0.6330023737507489 1107 24-25 0.4470033059070815 1107 26-27 0.5158587662866339 1107 28-29 0.33009219685433777 1107 30-31 0.5810266527893333 1107 32-33 0.5957633432375431 1107 34-35 0.6670336420522318 1107 36-37 0.6468703773262732 1107 38-39 0.6834124602050409 1107 40-41 0.7037894942888769 1107 42-43 0.7735441358874766 1107 44-45 0.8573821909621842 1107 46-47 0.8793666142412349 1107 48-49 0.7360804465817239 1107 50-51 0.7444720149694959 1107 52-53 0.7827849693183779 1107 54-55 0.9433796202122267 1107 56-57 0.9223450560008928 1107 58-59 0.9303175277601383 1107 60-61 0.9175826861287248 1107 62-63 0.9621062550652297 1107 64-65 0.9407298489556766 1107 66-67 0.9415829349017883 1107 68-69 0.9701957565135118 1107 70-71 1.0559002493494205 1107 72-73 0.9550585018242828 1107 74-75 1.0201837136886027 1107 76-77 1.0357491313378624 1107 78-79 1.0973152118634353 1107 80-81 1.0746173131737855 1107 82-83 1.1380690167733896 1107 84-85 1.105061397667619 1107 86-87 1.0817390304054832 1107 88-89 1.1720439740783313 1107 90-91 1.2533626437563932 1107 92-93 1.2960521666219407 1107 94-95 1.2315681513872256 1107 96-97 1.3385497374767468 1107 98-99 1.459312050706945 1107 100-101 1.4479601829361464 1108 1 0.11944133119175859 1108 2 0.22581614512172976 1108 3 0.029441575425469324 1108 4 0.14168208609640232 1108 5 0.08125338702856766 1108 6 0.1257534598048622 1108 7 -0.04130353987136459 1108 8 -0.10551414234192436 1108 9 -0.02248256620286071 1108 10-11 -0.005198299972541065 1108 12-13 -0.12459590668732545 1108 14-15 0.20722828812846927 1108 16-17 0.1635181315673293 1108 18-19 0.2017949204018592 1108 20-21 0.303475799970478 1108 22-23 0.2302096321531053 1108 24-25 0.2765499605844184 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52-53 0.39666247147389555 1302 54-55 0.5224549036271 1302 56-57 0.398468793243822 1302 58-59 0.634427969033311 1302 60-61 0.5440576054495168 1302 62-63 0.5005604143981444 1302 64-65 0.44153200451130914 1302 66-67 0.30610965033346815 1302 68-69 0.4397543272990916 1302 70-71 0.33320158138938893 1302 72-73 0.5253751137668417 1302 74-75 0.5134767007015171 1302 76-77 0.5363583423577829 1302 78-79 0.4358524198453644 1302 80-81 0.39500507221477577 1302 82-83 0.43356309356160594 1302 84-85 0.406957690075604 1302 86-87 0.3414143003391743 1302 88-89 0.3879290842728764 1302 90-91 0.39229000004277026 1302 92-93 0.37510653758629786 1302 94-95 0.39938973531285527 1302 96-97 0.28311978452912 1302 98-99 0.14241110703741455 1302 100-101 0.5549159197841043 1303 1 0.1747594462145834 1303 2 0.2795851918363823 1303 3 0.2522385270264529 1303 4 0.062255956774258436 1303 5 0.0838782377731846 1303 6 0.14642181732281756 1303 7 0.1729704943517092 1303 8 0.12084839171579631 1303 9 0.2273289102860261 1303 10-11 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-2.47768304683672 2316 96-97 -2.339822933977185 2316 98-99 -2.3693283115004498 2316 100-101 -2.1757113222294855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10333.0 3 3384.0 4 3304.0 5 3343.0 6 3519.0 7 3982.0 8 4798.0 9 5775.0 10 7001.0 11 8307.0 12 9994.0 13 11535.0 14 12781.0 15 14871.0 16 18359.0 17 23084.0 18 20563.0 19 15279.0 20 15319.0 21 16055.0 22 17679.0 23 19638.0 24 22383.0 25 25991.0 26 30425.0 27 35624.0 28 42148.0 29 51054.0 30 62516.0 31 77403.0 32 97656.0 33 126623.0 34 172083.0 35 241676.0 36 358413.0 37 520420.0 38 463974.0 39 84099.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21143509786459 18.967868205090994 14.723278737089519 23.097417959954903 2 14.962730910403755 20.194054997292255 41.02315121246766 23.820062879836332 3 18.72789925160592 24.826042083772766 29.93418418277284 26.511874481848487 4 12.597575647225145 16.88413261033619 37.83877324228405 32.679518500154614 5 13.78084217636824 37.20878538937813 34.34957713656424 14.660795297689388 6 31.26368788665988 36.52307087067276 17.990777142870037 14.222464099797316 7 28.027024658383386 32.81467865338604 21.285642228699295 17.872654459531283 8 25.75193215366968 35.15049173545949 20.149119013144286 18.948457097726546 9 26.120224375901856 17.002997296113897 20.611865199917546 36.2649131280667 10-11 24.412736831514284 26.86127543214787 27.909620138659687 20.81636759767816 12-13 25.4876627744152 24.210720486151917 28.255248559984793 22.04636817944809 14-15 22.54703044954122 25.41419363961637 26.557301895208084 25.48147401563432 16-17 22.000990352805193 28.80894183642554 26.917774199936545 22.272293610832723 18-19 21.953316228008983 27.411946626273732 29.250367872274897 21.384369273442385 20-21 22.606329478975496 26.508777490640462 29.388247122579557 21.49664590780448 22-23 22.35804027941863 26.54211922292803 29.276181960517718 21.82365853713562 24-25 22.19699731018515 26.72727412858289 29.27290783666814 21.802820724563823 26-27 22.08331845257675 26.93282231248837 29.0549372470663 21.92892198786858 28-29 21.840212503571806 26.83923495706315 29.394579128630085 21.925973410734965 30-31 22.10150164452941 26.62930959462007 29.3307961650014 21.93839259584912 32-33 21.850107953444407 26.85536230799897 29.246690395686446 22.047839342870176 34-35 22.240975444910287 26.85606628015398 29.01200191633711 21.890956358598622 36-37 22.161864169759234 26.701374060953093 29.020026735394776 22.1167350338929 38-39 21.96502913299113 26.857743902221266 29.173966595974594 22.00326036881301 40-41 22.240736848714487 26.65826114320884 29.15869445314406 21.942307554932608 42-43 22.084516510085685 26.784521284774872 28.90341179739227 22.227550407747177 44-45 22.235356209652775 26.90818208781406 28.68758573169049 22.16887597084267 46-47 22.25462020501502 27.03878010349981 28.404488199157445 22.30211149232772 48-49 22.349284990005742 27.18588568297856 28.17189184211046 22.292937484905238 50-51 22.260574450312745 27.216624838976706 28.120384821011623 22.402415889698926 52-53 22.50923875444882 27.27989981944421 28.014170068258622 22.19669135784835 54-55 22.34212269317834 27.197715864076542 28.02678833889294 22.433373103852176 56-57 22.28583071486343 27.531103512844112 27.944751898371102 22.238313873921356 58-59 22.42722012591857 27.22044547068711 28.03714548982264 22.315188913571678 60-61 22.430485903198303 27.11367440139908 28.28112100738486 22.174718688017755 62-63 22.29650015226537 27.254617776324054 28.252219126786297 22.19666294462428 64-65 22.234516101870756 27.23757589335386 28.140834608872943 22.387073395902434 66-67 22.227765579884505 27.512105991337826 27.917073959335898 22.34305446944177 68-69 22.041722476958167 27.359567647712286 28.166318490646614 22.432391384682937 70-71 22.13432793189345 27.25276724198842 28.078918449232027 22.533986376886105 72-73 22.19978214248603 27.20580732894925 28.05750731844534 22.536903210119384 74-75 22.092797748535332 27.41973068417405 27.872380169190592 22.615091398100027 76-77 22.20132240227643 27.420150283237664 27.845232322548874 22.53329499193703 78-79 22.225332302933726 27.36899014760268 27.874026316546352 22.531651232917245 80-81 22.120983514003083 27.538970951131397 27.799065471498608 22.54098006336691 82-83 22.140496291880936 27.469781362615564 27.865265188832765 22.52445715667073 84-85 22.230161460418792 27.407516021031775 27.659773252349346 22.702549266200087 86-87 22.18395849997603 27.33971048112469 27.864363574498803 22.611967444400477 88-89 22.299286639141094 27.274855468178554 27.728561097482874 22.697296795197477 90-91 22.255842291130833 27.37950444904047 27.784555680586454 22.58009757924225 92-93 22.231283968623796 27.29085931112303 27.755640420126625 22.722216300126544 94-95 22.235914135783087 27.18844890507518 27.716197592237098 22.859439366904635 96-97 22.216259514425825 27.211502301513285 27.67445621107735 22.897781972983545 98-99 22.059049514641814 27.178215205275414 27.70131375586866 23.061421524214115 100-101 22.16759275124327 27.175201609831724 27.692652895174923 22.96455274375008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 525.0 1 422.5 2 399.5 3 479.0 4 431.5 5 443.5 6 745.5 7 1542.5 8 2753.5 9 3292.0 10 2826.5 11 2485.0 12 2533.5 13 2663.5 14 2869.5 15 3153.5 16 3288.5 17 3342.0 18 3570.0 19 3884.0 20 4349.0 21 5061.5 22 6116.0 23 7508.0 24 9485.0 25 11978.0 26 15051.5 27 18480.0 28 21641.5 29 25500.5 30 30130.5 31 34880.0 32 39999.0 33 45521.5 34 52232.5 35 58132.0 36 64256.5 37 70251.5 38 75008.0 39 78315.0 40 81020.0 41 85745.5 42 88571.5 43 92439.5 44 97604.5 45 101166.5 46 102888.5 47 104835.5 48 108265.0 49 108062.0 50 105266.5 51 101659.0 52 95848.0 53 90959.0 54 86514.0 55 79488.0 56 72312.0 57 64129.0 58 56751.5 59 50584.5 60 43070.0 61 34239.5 62 25692.0 63 19550.5 64 14964.5 65 11146.5 66 7831.5 67 5756.5 68 3855.5 69 2155.5 70 1361.0 71 842.0 72 524.5 73 316.5 74 211.5 75 146.5 76 94.0 77 62.0 78 42.0 79 32.5 80 27.0 81 25.5 82 19.5 83 11.5 84 8.0 85 7.5 86 6.0 87 5.5 88 6.0 89 4.0 90 3.0 91 2.5 92 3.5 93 2.5 94 0.5 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05538386390749316 2 0.08961364682453948 3 0.06500277107188818 4 0.12140112909437614 5 0.09945799712560001 6 0.09754173046394318 7 0.10580797880834515 8 0.0929952938745221 9 0.11381121015997069 10-11 0.10360990822585645 12-13 0.0878664625153818 14-15 0.09028997623453601 16-17 0.10432381149196389 18-19 0.0831885174295725 20-21 0.06906074753186733 22-23 0.071503048179077 24-25 0.05923518415886227 26-27 0.05222765999417606 28-29 0.06406342466911523 30-31 0.046647942361704726 32-33 0.04698610706670299 34-35 0.04289055675061293 36-37 0.04813210967808598 38-39 0.048714504447805215 40-41 0.06259804428078942 42-43 0.05884065866969762 44-45 0.046441286153094674 46-47 0.035206703175930186 48-49 0.04311599988727843 50-51 0.026583503198474502 52-53 0.038644711010079186 54-55 0.04369839465699766 56-57 0.04942840771391266 58-59 0.05455723907305297 60-61 0.05219008613806513 62-63 0.059930300496914245 64-65 0.04181970185145176 66-67 0.05955456193580507 68-69 0.051551330584179524 70-71 0.024535728040429472 72-73 0.01921902740073457 74-75 0.028443409075964945 76-77 0.0418948495636736 78-79 0.06070056454718807 80-81 0.03930225349202025 82-83 0.05705590050442902 84-85 0.06162112402190555 86-87 0.0626544050649558 88-89 0.08724649388955165 90-91 0.06154597630968372 92-93 0.07114609654602327 94-95 0.07928083639403703 96-97 0.08660773833566604 98-99 0.11762495655522887 100-101 0.1131160938219187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2661425.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08149872019161 #Duplication Level Percentage of deduplicated Percentage of total 1 75.34475394398828 41.50101967938942 2 13.755959011983917 15.15397677427201 3 4.599972449072242 7.601201296994681 4 2.1131654613265916 4.655852826144551 5 1.101649861618574 3.034026272142137 6 0.7181118486054624 2.3732806121949728 7 0.4467581976165142 1.7225677763174396 8 0.337740149167053 1.4882586875281887 9 0.23564881473708757 1.1681900898620006 >10 1.208758121173231 12.070475502033716 >50 0.0917813371106912 3.438371154928309 >100 0.04317284941515174 4.083132517789219 >500 0.002186338754798892 0.7356949414902366 >1k 2.0496925825762483E-4 0.30027673732834526 >5k 6.832308608587495E-5 0.2769440418597936 >10k+ 6.832308608587495E-5 0.39673108972495497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10542 0.39610359112129784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7359 0.27650600712024576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4903 0.18422461651183106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.636078416637704E-5 0.0 22-23 0.0 0.0 0.0 7.514771222183605E-5 0.0 24-25 0.0 0.0 0.0 7.514771222183605E-5 0.0 26-27 0.0 0.0 0.0 1.502954244436721E-4 0.0 28-29 0.0 0.0 0.0 6.951163380519834E-4 0.0 30-31 0.0 0.0 0.0 0.001296298035826672 0.0 32-33 0.0 0.0 0.0 0.0023671529349878354 0.0 34-35 0.0 0.0 0.0 0.004320993452755573 0.0 36-37 0.0 0.0 0.0 0.006669359459687949 0.0 38-39 0.0 0.0 0.0 0.00852926533717839 0.0 40-41 0.0 0.0 0.0 0.01027644964633608 0.0 42-43 0.0 0.0 0.0 0.012812684933823046 0.0 44-45 0.0 0.0 0.0 0.015743445710474653 0.0 46-47 0.0 0.0 0.0 0.020665620861004916 0.0 48-49 0.0 0.0 0.0 0.02643320777403083 0.0 50-51 0.0 0.0 0.0 0.031918990766224856 0.0 52-53 0.0 0.0 0.0 0.04330386916783302 0.0 54-55 0.0 0.0 0.0 0.06297378284189861 0.0 56-57 0.0 0.0 0.0 0.09615149778783921 0.0 58-59 0.0 0.0 0.0 0.13417624017208826 0.0 60-61 0.0 0.0 0.0 0.17116770151328706 0.0 62-63 0.0 0.0 0.0 0.21689508440027427 0.0 64-65 0.0 0.0 0.0 0.2705693378547207 0.0 66-67 0.0 0.0 0.0 0.3471824304648825 0.0 68-69 0.0 1.8786928055459013E-5 0.0 0.4399147073466282 0.0 70-71 0.0 3.7573856110918025E-5 0.0 0.5279878260706201 0.0 72-73 0.0 3.7573856110918025E-5 0.0 0.6175638990390486 0.0 74-75 0.0 3.7573856110918025E-5 0.0 0.713884479179387 0.0 76-77 0.0 3.7573856110918025E-5 0.0 0.8297434644974027 0.0 78-79 0.0 3.7573856110918025E-5 0.0 0.9326770433132626 0.0 80-81 0.0 3.7573856110918025E-5 0.0 1.0452295292935174 0.0 82-83 0.0 3.7573856110918025E-5 0.0 1.1781282583578345 0.0 84-85 0.0 3.7573856110918025E-5 0.0 1.3500286500652847 0.0 86-87 0.0 3.7573856110918025E-5 0.0 1.5533032116253511 0.0 88-89 0.0 3.7573856110918025E-5 0.0 1.7844200005636077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 47.44101 1 GTATCAA 9625 0.0 43.013752 1 ATCAACG 12100 0.0 33.54578 3 TATCAAC 12195 0.0 33.481216 2 TCAACGC 12200 0.0 33.27144 4 CAACGCA 12435 0.0 32.71542 5 AACGCAG 12835 0.0 31.806934 6 CGCAGAG 14320 0.0 28.143982 8 ACGCAGA 14380 0.0 27.992445 7 GTACATG 9860 0.0 25.549881 1 GCAGAGT 15355 0.0 25.44316 9 TACATGG 10175 0.0 23.8246 2 ACATGGG 9895 0.0 23.72884 3 CGACCGT 105 0.002190197 22.631077 6 GAGTACT 8615 0.0 22.009256 12-13 AGAGTAC 13445 0.0 21.437498 10-11 GTACTTT 9085 0.0 20.89679 14-15 CATGGGG 5205 0.0 20.820333 4 AGTACTT 9090 0.0 20.519346 12-13 CAGAGTA 14800 0.0 20.18123 10-11 >>END_MODULE