FastQCFastQC Report
Thu 26 May 2016
SRR936791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661425
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105020.39460063687686103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80750.303408888095663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.21424612754445457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.046.944131
GTATCAA96850.041.4537351
ATCAACG123350.032.386613
TCAACGC124250.032.150814
TATCAAC127100.031.5064052
CAACGCA127950.031.2210885
AACGCAG131600.030.3895366
ACGCAGA149000.026.6813037
CGCAGAG150150.026.1922518
GCAGAGT163000.023.5737559
GTACATG96700.023.3876741
TACATGG98800.021.635772
GTGGTAT31600.021.5008451
ACATGGG94750.021.4572073
TGGTATC29850.021.1652852
AGAGTAC144750.021.11532810-11
AGTACTT97050.020.99566312-13
GAGTACT93550.020.96882412-13
GTACTTT100550.019.5566414-15
CAGAGTA157350.019.3037510-11
Produced by FastQC (version 0.11.5)