##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936791_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2661425 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.998330969311553 31.0 31.0 34.0 30.0 34.0 2 31.99190997304076 31.0 31.0 34.0 30.0 34.0 3 32.18035150342392 33.0 31.0 34.0 30.0 34.0 4 35.942221929981116 37.0 35.0 37.0 35.0 37.0 5 35.8540447316757 37.0 35.0 37.0 35.0 37.0 6 35.88872840677457 37.0 35.0 37.0 35.0 37.0 7 35.82143964229689 37.0 35.0 37.0 35.0 37.0 8 35.79398179546671 37.0 35.0 37.0 33.0 37.0 9 37.45573856110918 39.0 37.0 39.0 34.0 39.0 10-11 37.37318391463219 39.0 37.0 39.0 34.0 39.0 12-13 37.27361695332388 39.0 37.0 39.0 34.0 39.0 14-15 38.44961139239317 40.0 38.0 41.0 34.0 41.0 16-17 38.35667433799563 40.0 38.0 41.0 33.0 41.0 18-19 38.405445766835435 40.0 38.0 41.0 33.5 41.0 20-21 38.48885427919254 40.0 38.0 41.0 34.0 41.0 22-23 38.4232501385536 40.0 38.0 41.0 34.0 41.0 24-25 38.322161999680624 40.0 38.0 41.0 34.0 41.0 26-27 38.164243967047724 40.0 38.0 41.0 33.0 41.0 28-29 37.98696506570728 40.0 37.0 41.0 33.0 41.0 30-31 37.8638684163559 40.0 37.0 41.0 33.0 41.0 32-33 37.865466056717736 40.0 37.0 41.0 33.0 41.0 34-35 37.79545469062626 40.0 37.0 41.0 33.0 41.0 36-37 37.64585889138337 40.0 37.0 41.0 33.0 41.0 38-39 37.49658002761679 40.0 37.0 41.0 32.5 41.0 40-41 37.32397963496999 40.0 36.0 41.0 32.0 41.0 42-43 37.11565270484797 39.5 36.0 41.0 31.0 41.0 44-45 36.8126003926468 39.0 36.0 41.0 31.0 41.0 46-47 36.568039302253496 39.0 36.0 41.0 30.0 41.0 48-49 36.268809002695924 39.0 35.0 40.5 30.0 41.0 50-51 36.02229764130118 39.0 35.0 40.0 29.0 41.0 52-53 35.76329654226589 39.0 35.0 40.0 28.5 41.0 54-55 35.52198784485755 38.0 35.0 40.0 28.0 41.0 56-57 35.251196445513216 38.0 34.0 40.0 27.5 41.0 58-59 34.91776360408428 38.0 34.0 40.0 26.5 41.0 60-61 34.58611345425852 37.5 34.0 40.0 26.0 41.0 62-63 34.207577895300446 37.0 33.0 40.0 26.0 41.0 64-65 33.798854748865736 36.5 33.0 39.0 25.0 40.5 66-67 33.417378096320576 36.0 32.5 39.0 24.5 40.0 68-69 32.977017011563355 35.5 32.0 38.5 23.5 40.0 70-71 32.491144593591784 35.0 31.0 38.0 22.0 40.0 72-73 32.126861361864414 35.0 31.0 37.5 22.0 39.5 74-75 31.662971904149092 35.0 31.0 37.0 20.5 39.0 76-77 30.278864705937607 33.5 29.5 35.5 20.0 37.5 78-79 31.011082408859917 34.0 30.5 36.0 20.0 38.0 80-81 30.836227772715745 34.0 31.0 36.0 19.5 37.0 82-83 30.491396902035564 34.0 30.0 35.0 18.0 37.0 84-85 30.094677287543327 34.0 30.0 35.0 16.0 36.5 86-87 29.707449768451113 34.0 30.0 35.0 9.5 36.0 88-89 29.321915327315253 34.0 29.0 35.0 7.0 36.0 90-91 28.871509623603895 34.0 29.0 35.0 2.0 35.0 92-93 28.367446386804062 33.0 29.0 35.0 2.0 35.0 94-95 27.84137125787877 33.0 27.5 35.0 2.0 35.0 96-97 27.156417332819824 33.0 26.5 35.0 2.0 35.0 98-99 26.337807189757367 32.5 25.0 34.0 2.0 35.0 100-101 23.199869430850015 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35717642053141674 1101 2 0.2098722500335377 1101 3 0.23215772212179075 1101 4 0.2717461801386065 1101 5 0.2704376003715936 1101 6 0.2336203040012279 1101 7 0.2680729629167615 1101 8 0.25809744367190746 1101 9 0.39613461218655033 1101 10-11 0.4522017496591957 1101 12-13 0.3600783898087485 1101 14-15 0.5035308967571552 1101 16-17 0.6187733229627028 1101 18-19 0.5690904692415231 1101 20-21 0.49572058107680306 1101 22-23 0.543947024632125 1101 24-25 0.5150425294493388 1101 26-27 0.5334390397955531 1101 28-29 0.448389540551247 1101 30-31 0.4643444189158643 1101 32-33 0.5328371914652266 1101 34-35 0.5668885866527873 1101 36-37 0.5720453248553596 1101 38-39 0.5536633646214923 1101 40-41 0.5205136492718552 1101 42-43 0.6724702556683084 1101 44-45 0.7679937021800853 1101 46-47 0.7229862851861881 1101 48-49 0.4586967210267545 1101 50-51 0.36004167852993874 1101 52-53 0.254338181957408 1101 54-55 0.39277839682838334 1101 56-57 0.46265075786403287 1101 58-59 0.5192892508740456 1101 60-61 0.47686764616227606 1101 62-63 0.5259850841875959 1101 64-65 0.4858143856293182 1101 66-67 0.5337317663179704 1101 68-69 0.3305914361494615 1101 70-71 0.3862488572607603 1101 72-73 0.5345227024886512 1101 74-75 0.406353427569325 1101 76-77 0.4150950122498607 1101 78-79 0.4843674327472911 1101 80-81 0.6339810783710895 1101 82-83 0.8542247616269556 1101 84-85 0.8072106588708081 1101 86-87 0.5897121559763114 1101 88-89 0.674636678845367 1101 90-91 0.8048994369067017 1101 92-93 0.6700543574123046 1101 94-95 0.643514317746213 1101 96-97 0.6659010769497264 1101 98-99 0.9279849051747782 1101 100-101 1.0291128300997983 1102 1 0.2768943861888644 1102 2 0.16189418544117373 1102 3 0.28506239118394916 1102 4 0.23169687381096082 1102 5 0.2956692273192729 1102 6 0.23587400063303932 1102 7 0.27133722442291486 1102 8 0.28572402612035575 1102 9 0.3622088509731185 1102 10-11 0.3517254709441673 1102 12-13 0.37431236928055966 1102 14-15 0.5047682546254961 1102 16-17 0.46854572430976305 1102 18-19 0.5407784053044296 1102 20-21 0.42004530460150846 1102 22-23 0.3225677902229691 1102 24-25 0.42065837514954296 1102 26-27 0.4605548344706847 1102 28-29 0.3424295442035685 1102 30-31 0.2875183929091918 1102 32-33 0.47267702646627896 1102 34-35 0.43962602777254745 1102 36-37 0.4713618575140899 1102 38-39 0.4563756154714653 1102 40-41 0.523302169168943 1102 42-43 0.4778901919675391 1102 44-45 0.5316357908777647 1102 46-47 0.6190627261072237 1102 48-49 0.5865388172439197 1102 50-51 0.6559373832935265 1102 52-53 0.6771093264765042 1102 54-55 0.6128105694757195 1102 56-57 0.7857763923204573 1102 58-59 0.6725324309710388 1102 60-61 0.6468426170242765 1102 62-63 0.7401832118361966 1102 64-65 0.7225414230065184 1102 66-67 0.7863551261306228 1102 68-69 0.4954250944319014 1102 70-71 0.5691063147085416 1102 72-73 0.5661531413579368 1102 74-75 0.5052233566176305 1102 76-77 0.47953094356475034 1102 78-79 0.6107179856978853 1102 80-81 0.5535220676128567 1102 82-83 0.5770833201419698 1102 84-85 0.5295130441584028 1102 86-87 0.6017516547490267 1102 88-89 0.7830441328019582 1102 90-91 0.9293672991712469 1102 92-93 0.8985497087601715 1102 94-95 0.8623076554838143 1102 96-97 1.058350844114294 1102 98-99 1.0656138777447772 1102 100-101 1.2888759395136518 1103 1 0.12461977864244744 1103 2 -0.1094213587576327 1103 3 0.056846575749709416 1103 4 0.17846561157133323 1103 5 0.23129950934129084 1103 6 0.19847388124755838 1103 7 0.3267022402886113 1103 8 0.3261941326247708 1103 9 0.25577722010464043 1103 10-11 0.37750906905494475 1103 12-13 0.31881513923311644 1103 14-15 0.39156280687758027 1103 16-17 0.4545687217230707 1103 18-19 0.45461848112468317 1103 20-21 0.37142949545071957 1103 22-23 0.4617516229157417 1103 24-25 0.46492708672888483 1103 26-27 0.477677487341694 1103 28-29 0.3896454538895 1103 30-31 0.40251724094404295 1103 32-33 0.4940554051276891 1103 34-35 0.44078061102297994 1103 36-37 0.5880051357974523 1103 38-39 0.6193378414870168 1103 40-41 0.47352392245990416 1103 42-43 0.5050896599690944 1103 44-45 0.6748274150439002 1103 46-47 0.6295576522243707 1103 48-49 0.6132570779859918 1103 50-51 0.4458281036906442 1103 52-53 0.6080212639771574 1103 54-55 0.5982722456897349 1103 56-57 0.6172988713494547 1103 58-59 0.684217546534093 1103 60-61 0.725925286232247 1103 62-63 0.7681626126048755 1103 64-65 0.7039447783668251 1103 66-67 0.6731467984698511 1103 68-69 0.6981259290830124 1103 70-71 0.5901757572621378 1103 72-73 0.714689621999689 1103 74-75 0.6787098314995497 1103 76-77 0.6060075534360401 1103 78-79 0.6660557732964563 1103 80-81 0.6907334218002497 1103 82-83 0.7166590827952568 1103 84-85 0.876510865897032 1103 86-87 0.7257525867935506 1103 88-89 1.0202664830203076 1103 90-91 1.2019246094373521 1103 92-93 1.2767846363434536 1103 94-95 1.328798036574831 1103 96-97 1.6246995858541773 1103 98-99 1.7311549348298847 1103 100-101 1.5383661949357474 1104 1 0.24724741125957195 1104 2 0.15570436168663448 1104 3 0.24234269864533786 1104 4 0.17067465550589844 1104 5 0.18263897527678807 1104 6 0.2530399715588132 1104 7 0.3308604616345008 1104 8 0.31704785678156355 1104 9 0.27175665945608074 1104 10-11 0.29908038693445604 1104 12-13 0.33170491670711755 1104 14-15 0.36397987737101545 1104 16-17 0.4450655375680981 1104 18-19 0.4736970316635265 1104 20-21 0.3601926253981489 1104 22-23 0.4320871547885261 1104 24-25 0.319034334297136 1104 26-27 0.2993715675064905 1104 28-29 0.459109431282549 1104 30-31 0.40850252574362145 1104 32-33 0.419764930189622 1104 34-35 0.4437306987178218 1104 36-37 0.38074554592900967 1104 38-39 0.3051348330367105 1104 40-41 0.4389700270081178 1104 42-43 0.43974663164373595 1104 44-45 0.5471938792328572 1104 46-47 0.5809801933433008 1104 48-49 0.6229652596710267 1104 50-51 0.6181964446175527 1104 52-53 0.6424048093687631 1104 54-55 0.6426673349883387 1104 56-57 0.6399278688669057 1104 58-59 0.7161806905504946 1104 60-61 0.7265179023314587 1104 62-63 0.7960852579287021 1104 64-65 0.5541705250066755 1104 66-67 0.7135594629600277 1104 68-69 0.6609337731651976 1104 70-71 0.6452040763191391 1104 72-73 0.5741812701220823 1104 74-75 0.5198077415746702 1104 76-77 0.48740677050821546 1104 78-79 0.5485078666073449 1104 80-81 0.6765605723975767 1104 82-83 0.7521888163614641 1104 84-85 0.7722445549443897 1104 86-87 0.7079193109435202 1104 88-89 0.8987584623935163 1104 90-91 0.9625289006839814 1104 92-93 1.0135855908287539 1104 94-95 0.9506261525766604 1104 96-97 1.128249901592369 1104 98-99 1.1341611146786015 1104 100-101 1.0698816122723969 1105 1 0.14917616160198577 1105 2 -0.015471525025478883 1105 3 0.03412016942766627 1105 4 0.05687345848642167 1105 5 0.1337315790733271 1105 6 0.1375330447824794 1105 7 0.14517844891805964 1105 8 0.0703169312822638 1105 9 0.1818683930979006 1105 10-11 0.13990478137937146 1105 12-13 0.17780442133208396 1105 14-15 0.2842172711241986 1105 16-17 0.2786103955805217 1105 18-19 0.2022301999813365 1105 20-21 0.22443010849095657 1105 22-23 0.18013439442430723 1105 24-25 0.16722070724667049 1105 26-27 0.21411339243894645 1105 28-29 0.27857338044830726 1105 30-31 0.3044338288341777 1105 32-33 0.2506403073374699 1105 34-35 0.2238110518195171 1105 36-37 0.19418550872168083 1105 38-39 0.2433388177149638 1105 40-41 0.1485439492997358 1105 42-43 0.21869992607491895 1105 44-45 0.3389242496441014 1105 46-47 0.3026780270546894 1105 48-49 0.36412763376621626 1105 50-51 0.41690529009350996 1105 52-53 0.4034050451990723 1105 54-55 0.37526584997998924 1105 56-57 0.4645750923427343 1105 58-59 0.3958419357476757 1105 60-61 0.5325638294631787 1105 62-63 0.36923614137268856 1105 64-65 0.3630438539124441 1105 66-67 0.3434607755297847 1105 68-69 0.5613350788374163 1105 70-71 0.3563397996185742 1105 72-73 0.43571767503024006 1105 74-75 0.32757982833132004 1105 76-77 0.31148228613246687 1105 78-79 0.3210237818687176 1105 80-81 0.5115938546890852 1105 82-83 0.6554957616626247 1105 84-85 0.6822273373610379 1105 86-87 0.5840276641319271 1105 88-89 0.6777285736935958 1105 90-91 0.8260649364093204 1105 92-93 0.9412176771518439 1105 94-95 0.7779860158344931 1105 96-97 0.8670179082875862 1105 98-99 0.7721652019660361 1105 100-101 0.9540730329102374 1106 1 0.39633945565643103 1106 2 0.30636873099936324 1106 3 0.26015801972220487 1106 4 0.2242085967447096 1106 5 0.2285630443905049 1106 6 0.2930510161826092 1106 7 0.35259148083039804 1106 8 0.3427180348986809 1106 9 0.4419932029941478 1106 10-11 0.4278010107391452 1106 12-13 0.4555775010904384 1106 14-15 0.6241497967425502 1106 16-17 0.6913685437540806 1106 18-19 0.6685541080054307 1106 20-21 0.5337642122010848 1106 22-23 0.5963709709406615 1106 24-25 0.6425055584953228 1106 26-27 0.6373936775078732 1106 28-29 0.731516640426122 1106 30-31 0.7650850628759756 1106 32-33 0.6912849149581533 1106 34-35 0.6253430243014222 1106 36-37 0.637242045081031 1106 38-39 0.8478849263159631 1106 40-41 0.7943795240310791 1106 42-43 0.8618001466358649 1106 44-45 0.915331312612075 1106 46-47 0.7745482790726825 1106 48-49 0.7316221603276105 1106 50-51 0.7385034382375579 1106 52-53 0.9080020695080009 1106 54-55 0.9780710841147666 1106 56-57 0.911903309016374 1106 58-59 0.6972663211249781 1106 60-61 0.8286601442727815 1106 62-63 0.8467858561340407 1106 64-65 0.8326991988442316 1106 66-67 0.9774585219562724 1106 68-69 0.8081144389376504 1106 70-71 0.8378445580235407 1106 72-73 0.9429277820196376 1106 74-75 0.8369567307524726 1106 76-77 0.8372353036260698 1106 78-79 0.9121848149705656 1106 80-81 0.9190096906549066 1106 82-83 1.015721074738888 1106 84-85 1.00066930193994 1106 86-87 1.0070393503937645 1106 88-89 1.2381500967226202 1106 90-91 1.4098212759683868 1106 92-93 1.3408121224694796 1106 94-95 1.302825354638685 1106 96-97 1.6951294299219661 1106 98-99 1.7577785628381228 1106 100-101 1.9378672271912976 1107 1 0.21993344678592308 1107 2 0.19526847177492002 1107 3 0.18166315831662416 1107 4 0.1716592398772505 1107 5 0.252772699223641 1107 6 0.1264858598724885 1107 7 0.15078617905073344 1107 8 0.13798995830968153 1107 9 0.28401324552449836 1107 10-11 0.19349679527854846 1107 12-13 0.23953316751610032 1107 14-15 0.29170993541157486 1107 16-17 0.3434355794753259 1107 18-19 0.36966760200999005 1107 20-21 0.28433857091110326 1107 22-23 0.37917701562239614 1107 24-25 0.31349715416126145 1107 26-27 0.369000520888477 1107 28-29 0.27676448621357963 1107 30-31 0.354307918577085 1107 32-33 0.3047052171198885 1107 34-35 0.3337186912968093 1107 36-37 0.39236311822410386 1107 38-39 0.34594899495052545 1107 40-41 0.40895690860882894 1107 42-43 0.34253985644736673 1107 44-45 0.4697291075087051 1107 46-47 0.599494686578204 1107 48-49 0.5497739302929361 1107 50-51 0.7052643354534638 1107 52-53 0.6706387524117972 1107 54-55 0.5253665452679499 1107 56-57 0.5383379623460698 1107 58-59 0.6294332122900457 1107 60-61 0.6467526810233792 1107 62-63 0.5486144442538432 1107 64-65 0.38834111733118704 1107 66-67 0.39540446780446104 1107 68-69 0.44984166139273185 1107 70-71 0.5149744781672254 1107 72-73 0.506261653425689 1107 74-75 0.6783161646818847 1107 76-77 0.6136563694893375 1107 78-79 0.7102117611094911 1107 80-81 0.8440000935176393 1107 82-83 0.7991391206125265 1107 84-85 0.7824077922706856 1107 86-87 0.6835281049001232 1107 88-89 0.7481661620365117 1107 90-91 0.8549107294266811 1107 92-93 1.0320354782616938 1107 94-95 1.0920665681147383 1107 96-97 1.1419038648664888 1107 98-99 1.089366551065634 1107 100-101 1.030342257678317 1108 1 -0.00700239501233213 1108 2 -0.09537792999590877 1108 3 -0.02715082857631046 1108 4 0.07039833652335403 1108 5 0.10673090861999412 1108 6 0.08065316458207406 1108 7 0.11588213091481947 1108 8 0.09775911666612558 1108 9 0.11724129361707014 1108 10-11 0.12336011506252476 1108 12-13 0.1539892031279706 1108 14-15 0.1511574026455449 1108 16-17 0.21544717247039102 1108 18-19 0.200011696108497 1108 20-21 0.17945834870115362 1108 22-23 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 19.0 5 71.0 6 144.0 7 292.0 8 762.0 9 1467.0 10 2402.0 11 3599.0 12 5471.0 13 7528.0 14 9852.0 15 13048.0 16 17132.0 17 22991.0 18 20752.0 19 14193.0 20 13262.0 21 14197.0 22 15129.0 23 17064.0 24 19995.0 25 24128.0 26 29437.0 27 36991.0 28 46419.0 29 57757.0 30 71409.0 31 89095.0 32 111772.0 33 144012.0 34 191407.0 35 265297.0 36 393249.0 37 572417.0 38 399619.0 39 29038.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.90982548783961 19.934363465410605 15.359760813342863 22.796050233406913 2 15.357315835492946 21.142071507129636 39.87845298469916 23.62215967267826 3 18.962027323613746 25.024977821498094 30.160357801891593 25.85263705299657 4 13.200547076372763 17.605300929578945 37.16264249911701 32.03150949493128 5 13.821132712678116 37.49309099298447 34.310457615801724 14.375318678535692 6 31.505096721032444 36.9702680940266 17.460172532151105 14.06446265278985 7 28.039200067332377 32.97836045665779 21.08078231803726 17.901657157972576 8 25.959025709911042 35.576505067773844 19.80807274298543 18.656396479329683 9 26.13006190292794 16.87663563692383 20.417746132241188 36.575556327907044 10-11 24.498369291581188 27.156968836185197 27.594498425652468 20.750163446581148 12-13 25.57610002372804 24.36277632689608 28.192759585760697 21.868364063615186 14-15 22.51301464441042 25.649229266276524 26.426763857707808 25.41099223160525 16-17 21.942850164855294 29.068375024657843 26.895854664324563 22.0929201461623 18-19 21.92680612829593 27.46688334257024 29.362803761143 21.243506767990834 20-21 22.523813943316974 26.615348312386033 29.602147172067223 21.25869057222977 22-23 22.319452924655025 26.57067172661262 29.42106202504298 21.688813323689377 24-25 22.20667654267176 26.836631649746884 29.406340620722887 21.550351186858464 26-27 22.122282255923675 27.03769159970098 29.190600875374408 21.649425269000936 28-29 21.859395801292806 26.96358567835863 29.467584599204237 21.709433921144324 30-31 22.11166117097425 26.72653961906011 29.48128793300773 21.680511276957905 32-33 21.90564579080806 26.946399602465988 29.33361388663705 21.8143407200889 34-35 22.270781245420583 26.912100778322113 29.155610314402452 21.661507661854852 36-37 22.272604214163984 26.81307066060279 29.0630320991217 21.851293026111524 38-39 22.04622632204369 26.99483817029811 29.250017613158676 21.70891789449953 40-41 22.313015172440746 26.771823077057483 29.216750951741545 21.698410798760232 42-43 22.1298071702357 26.915851013183843 28.963429641127497 21.990912175452955 44-45 22.27225631550223 27.034416939157197 28.80316148504128 21.890165260299295 46-47 22.41390129036282 27.193526618476888 28.358937440210536 22.033634650949754 48-49 22.50008360201832 27.382760850508696 28.11956704299473 21.997588504478248 50-51 22.355300595827984 27.37804689033778 28.103166633632952 22.163485880201282 52-53 22.640753694819125 27.424014957566435 27.934555386450455 22.000675961163985 54-55 22.491217318457903 27.379365061770443 27.98623853986639 22.14317907990526 56-57 22.440846361167754 27.69070010449321 27.886084743578326 21.982368790760713 58-59 22.603399464828428 27.426688201783318 27.9116980058725 22.058214327515756 60-61 22.577906482161993 27.335635132840057 28.119133717793222 21.96732466720473 62-63 22.485397860168895 27.409282621152204 28.113191241534143 21.99212827714476 64-65 22.37098571984397 27.36474750983544 28.08806424989963 22.176202520420958 66-67 22.328906944382602 27.642640247731993 27.870413006267885 22.15803980161752 68-69 22.21484730924223 27.55065425476953 27.963947885061575 22.270550550926664 70-71 22.289594385551467 27.397076521960656 28.006262824455675 22.307066268032205 72-73 22.385883502259126 27.397071117916155 27.92346205510206 22.293583324722658 74-75 22.295671078545293 27.599074702221206 27.68027215613057 22.424982063102934 76-77 22.39118705103551 27.588842271855214 27.68253349174608 22.337437185363203 78-79 22.393692988589923 27.553121098181084 27.672230490576645 22.380955422652352 80-81 22.279546167204344 27.777972963096637 27.60942678706198 22.333054082637037 82-83 22.339350063848347 27.612714359762748 27.642961774219394 22.404973802169508 84-85 22.393026063022823 27.607133631115932 27.47485052158924 22.524989784272005 86-87 22.3698105664295 27.515596692920575 27.678162638464272 22.43643010218565 88-89 22.459365803116597 27.480430863860057 27.519940181802983 22.540263151220362 90-91 22.373150423458156 27.623505497065477 27.536597004606563 22.466747074869804 92-93 22.366518381415666 27.519510698124254 27.515264851586057 22.59870606887402 94-95 22.35502321601177 27.416874654202132 27.432806014088357 22.795296115697745 96-97 22.39461783995927 27.447287784367912 27.370372944914017 22.787721430758797 98-99 22.205678868772797 27.483925026983407 27.315928055513023 22.99446804873077 100-101 22.02857137489409 27.685620940818858 27.300617530965788 22.985190153321273 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 694.0 1 496.0 2 351.0 3 438.0 4 405.5 5 387.5 6 704.0 7 1585.0 8 2643.5 9 3113.0 10 2817.5 11 2498.0 12 2551.5 13 2746.5 14 3008.5 15 3210.5 16 3408.5 17 3642.0 18 3915.5 19 4185.5 20 4678.0 21 5427.5 22 6529.5 23 7905.5 24 9680.0 25 12160.5 26 15278.0 27 18759.5 28 21776.0 29 25479.0 30 30110.0 31 34865.0 32 40027.5 33 45820.5 34 52531.5 35 57712.5 36 63593.0 37 70262.0 38 75352.0 39 78460.0 40 81252.5 41 86213.0 42 89338.0 43 92799.5 44 98176.5 45 101558.0 46 102357.0 47 104501.0 48 108821.0 49 108671.5 50 105444.0 51 101817.5 52 95919.5 53 90997.5 54 85942.5 55 78797.0 56 71691.0 57 63576.5 58 56049.0 59 49992.0 60 42600.5 61 33484.5 62 25339.5 63 19308.0 64 14698.5 65 11081.0 66 7731.5 67 5564.5 68 3685.0 69 1993.5 70 1234.0 71 763.0 72 450.0 73 282.0 74 183.0 75 109.0 76 66.5 77 34.5 78 15.0 79 9.0 80 6.0 81 3.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015292559437143636 2 0.0025550222155424256 3 0.0024047267910987536 4 1.8786928055459013E-4 5 0.001653249668880393 6 3.005908488873442E-4 7 3.7573856110918025E-5 8 0.0 9 0.0 10-11 1.8786928055459013E-4 12-13 6.199686258301473E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024047267910987536 22-23 0.0 24-25 0.003475581690259917 26-27 0.001859905877490442 28-29 0.003081056201095278 30-31 0.015085903228533587 32-33 6.575424819410654E-4 34-35 0.0022168575105441634 36-37 0.0034004339780380807 38-39 0.0023483660069323767 40-41 0.004490075805254704 42-43 0.002329579078876917 44-45 6.575424819410654E-4 46-47 1.1272156833275406E-4 48-49 2.2544313666550812E-4 50-51 9.393464027729506E-5 52-53 0.0011084287552720817 54-55 0.002987121560817983 56-57 3.005908488873442E-4 58-59 0.0013338718919375897 60-61 0.0010896418272166226 62-63 0.0 64-65 1.690823524991311E-4 66-67 1.8786928055459013E-5 68-69 0.0 70-71 2.2544313666550812E-4 72-73 0.0 74-75 4.320993452755573E-4 76-77 8.641986905511146E-4 78-79 2.2544313666550812E-4 80-81 0.005128831359140311 82-83 0.00152174117249218 84-85 0.003287712409705327 86-87 0.001634462740824934 88-89 0.0010332810430502457 90-91 1.8786928055459013E-4 92-93 1.8786928055459013E-5 94-95 2.818039208318852E-4 96-97 0.001502954244436721 98-99 0.0025362352874869665 100-101 7.514771222183605E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2661425.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.00101244265423 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73735065773515 39.08154239698978 2 14.391264091191871 15.255031343255668 3 5.038767420722975 8.011793242841373 4 2.2603098124895507 4.791948339840483 5 1.2632301073856715 3.3476237319741697 6 0.7543633212263544 2.3989211864759987 7 0.4911991063930871 1.822383496483245 8 0.35442193854332316 1.5027777259747441 9 0.23898128397591012 1.1399625005011824 >10 1.3214345644493277 12.789115617701196 >50 0.09906968294644342 3.619136174159385 >100 0.047123408038952506 4.444086103138747 >500 0.001987683921091449 0.6680271840878943 >1k 2.839548458652632E-4 0.21415999615733194 >5k 1.419774229326316E-4 0.5183559850772245 >10k+ 7.09887114663158E-5 0.3951349753415847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10502 0.39460063687686103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8075 0.303408888095663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5702 0.21424612754445457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8786928055459013E-5 0.0 16-17 0.0 0.0 0.0 3.7573856110918025E-5 0.0 18-19 0.0 0.0 0.0 3.7573856110918025E-5 0.0 20-21 0.0 0.0 0.0 7.514771222183605E-5 0.0 22-23 0.0 0.0 0.0 1.1272156833275408E-4 0.0 24-25 0.0 0.0 0.0 1.315084963882131E-4 0.0 26-27 0.0 0.0 0.0 2.0665620861004915E-4 0.0 28-29 0.0 0.0 0.0 6.951163380519834E-4 0.0 30-31 0.0 0.0 0.0 0.001409019604159426 0.0 32-33 0.0 0.0 0.0 0.0025174483594315074 0.0 34-35 0.0 0.0 0.0 0.00437735423692195 0.0 36-37 0.0 0.0 0.0 0.006744507171909785 0.0 38-39 0.0 0.0 0.0 0.008698347689677522 0.0 40-41 0.0 0.0 0.0 0.010389171214668834 0.0 42-43 0.0 0.0 0.0 0.012850258789933964 0.0 44-45 0.0 0.0 0.0 0.015762232638530112 0.0 46-47 0.0 0.0 0.0 0.020628047004893995 0.0 48-49 0.0 0.0 0.0 0.02641442084597537 0.0 50-51 0.0 0.0 0.0 0.03182505612594756 0.0 52-53 0.0 0.0 0.0 0.04311599988727843 0.0 54-55 0.0 0.0 0.0 0.0628422743455104 0.0 56-57 0.0 0.0 0.0 0.09590726772311825 0.0 58-59 0.0 0.0 0.0 0.13395079703542273 0.0 60-61 0.0 0.0 0.0 0.1709046845205106 1.8786928055459013E-5 62-63 0.0 0.0 0.0 0.21659449355138694 3.7573856110918025E-5 64-65 0.0 0.0 0.0 0.2701184515813897 3.7573856110918025E-5 66-67 0.0 0.0 0.0 0.34643095334266416 3.7573856110918025E-5 68-69 0.0 0.0 0.0 0.43957654264162993 3.7573856110918025E-5 70-71 0.0 0.0 0.0 0.5278751045022874 3.7573856110918025E-5 72-73 0.0 0.0 0.0 0.6171881604779395 3.7573856110918025E-5 74-75 0.0 0.0 0.0 0.7137153968268879 3.7573856110918025E-5 76-77 0.0 0.0 0.0 0.8295180213607372 3.7573856110918025E-5 78-79 0.0 0.0 0.0 0.9323576655363198 3.7573856110918025E-5 80-81 0.0 0.0 0.0 1.0445344129554655 3.7573856110918025E-5 82-83 0.0 0.0 0.0 1.1768319603220079 3.7573856110918025E-5 84-85 0.0 0.0 0.0 1.3488450735977906 3.7573856110918025E-5 86-87 0.0 0.0 0.0 1.5525705214311882 3.7573856110918025E-5 88-89 0.0 0.0 0.0 1.7825788816141728 3.7573856110918025E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5435 0.0 46.94413 1 GTATCAA 9685 0.0 41.453735 1 ATCAACG 12335 0.0 32.38661 3 TCAACGC 12425 0.0 32.15081 4 TATCAAC 12710 0.0 31.506405 2 CAACGCA 12795 0.0 31.221088 5 AACGCAG 13160 0.0 30.389536 6 ACGCAGA 14900 0.0 26.681303 7 CGCAGAG 15015 0.0 26.192251 8 GCAGAGT 16300 0.0 23.573755 9 GTACATG 9670 0.0 23.387674 1 TACATGG 9880 0.0 21.63577 2 GTGGTAT 3160 0.0 21.500845 1 ACATGGG 9475 0.0 21.457207 3 TGGTATC 2985 0.0 21.165285 2 AGAGTAC 14475 0.0 21.115328 10-11 AGTACTT 9705 0.0 20.995663 12-13 GAGTACT 9355 0.0 20.968824 12-13 GTACTTT 10055 0.0 19.55664 14-15 CAGAGTA 15735 0.0 19.30375 10-11 >>END_MODULE