FastQCFastQC Report
Thu 26 May 2016
SRR936790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543406
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101110.3975377898770389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71180.2798609423741235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.19116098648819732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.047.6693461
GTATCAA89500.043.4345441
ATCAACG108800.035.161873
TCAACGC110400.034.6960074
TATCAAC112250.034.2511942
CAACGCA112050.034.0612035
AACGCAG115750.033.010886
ACGCAGA133850.028.2984077
CGCAGAG134600.027.8245948
GTACATG93850.024.8629231
GCAGAGT144150.024.8272179
GTGGTAT25550.023.9940621
TACATGG95700.023.3895842
TGGTATC26000.022.665322
ACATGGG94200.022.5507813
GAGTACT80700.021.87712712-13
AGAGTAC128000.020.94028910-11
CAGAGTA136800.020.53122710-11
GTACTTT87000.020.23769814-15
CATGGGG50200.019.7859124
Produced by FastQC (version 0.11.5)