##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936790_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543406 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61604989529788 33.0 31.0 34.0 30.0 34.0 2 31.747620710181543 33.0 31.0 34.0 30.0 34.0 3 31.760825444305784 33.0 31.0 34.0 30.0 34.0 4 35.34425412222822 37.0 35.0 37.0 33.0 37.0 5 35.34732048284859 37.0 35.0 37.0 33.0 37.0 6 35.40223346174382 37.0 35.0 37.0 33.0 37.0 7 35.332609893976816 37.0 35.0 37.0 33.0 37.0 8 35.27939109996595 37.0 35.0 37.0 33.0 37.0 9 36.902192571693234 39.0 37.0 39.0 33.0 39.0 10-11 36.70307552156439 39.0 37.0 39.0 32.0 39.0 12-13 36.62423655523341 39.0 37.0 39.0 32.0 39.0 14-15 37.77275806536589 40.0 38.0 41.0 32.0 41.0 16-17 37.70001191315897 40.0 37.5 41.0 32.0 41.0 18-19 37.65140858360796 40.0 37.0 41.0 32.0 41.0 20-21 37.56645871716903 40.0 37.0 41.0 32.0 41.0 22-23 37.45652994449176 40.0 37.0 41.0 32.0 41.0 24-25 37.29561678316399 40.0 37.0 41.0 31.5 41.0 26-27 37.405907865279865 40.0 37.0 41.0 32.0 41.0 28-29 37.359099962805786 40.0 37.0 41.0 31.5 41.0 30-31 37.21794652525 40.0 37.0 41.0 31.0 41.0 32-33 37.032197966034516 40.0 36.0 41.0 31.0 41.0 34-35 36.87274996599049 39.0 36.0 41.0 30.0 41.0 36-37 36.70403191625718 39.0 36.0 41.0 30.0 41.0 38-39 36.54822195119458 39.0 35.5 41.0 30.0 41.0 40-41 36.378976459126065 39.0 35.0 41.0 30.0 41.0 42-43 36.17640066116066 39.0 35.0 41.0 30.0 41.0 44-45 35.93978212680162 39.0 35.0 40.0 28.5 41.0 46-47 35.61810992818292 39.0 35.0 40.0 27.5 41.0 48-49 35.31687135282374 38.5 35.0 40.0 26.5 41.0 50-51 34.68807536036323 38.0 34.0 39.5 25.0 40.5 52-53 34.9323285389749 38.5 34.5 40.0 26.0 40.5 54-55 35.31423433773452 39.0 35.0 40.0 26.5 41.0 56-57 35.209538508598314 39.0 35.0 41.0 26.0 41.0 58-59 34.966326453582326 38.5 34.5 40.0 26.0 41.0 60-61 34.68687578782153 38.0 34.0 40.0 26.0 41.0 62-63 34.3674079167856 38.0 34.0 40.0 24.5 41.0 64-65 34.047202058971315 37.0 34.0 40.0 23.5 41.0 66-67 33.67958969193278 37.0 33.5 39.5 23.0 41.0 68-69 33.29971817318981 36.0 33.0 39.0 22.0 41.0 70-71 32.88711869044895 36.0 32.5 39.0 21.5 40.5 72-73 32.487943922441005 35.0 32.0 38.5 20.5 40.0 74-75 32.053078037875196 35.0 32.0 37.5 19.5 39.5 76-77 31.615390543232188 35.0 31.0 37.0 18.0 39.0 78-79 31.173255665827632 35.0 31.0 36.5 16.0 39.0 80-81 30.73242081681021 35.0 31.0 36.0 12.0 38.0 82-83 30.326558756250478 34.0 30.5 35.5 7.5 37.0 84-85 29.961320174600516 34.0 30.0 35.0 7.0 37.0 86-87 29.62690620372839 34.0 30.0 35.0 2.0 36.0 88-89 29.27704208451187 34.0 29.5 35.0 2.0 36.0 90-91 28.919817363016364 34.0 29.0 35.0 2.0 36.0 92-93 28.538126237022325 34.0 29.0 35.0 2.0 35.0 94-95 28.11051558422053 34.0 29.0 35.0 2.0 35.0 96-97 27.620476439860564 34.0 27.5 35.0 2.0 35.0 98-99 27.005873423275716 34.0 26.5 35.0 2.0 35.0 100-101 24.754261018492528 31.5 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31737485002793875 1101 2 0.4097231558674146 1101 3 0.34518214045930407 1101 4 0.3332266505883439 1101 5 0.35832761106370725 1101 6 0.32053815906555627 1101 7 0.22879958092286046 1101 8 0.2240515851039646 1101 9 0.2288172445089245 1101 10-11 0.41987110740165434 1101 12-13 0.3898405654705144 1101 14-15 0.6930012602758069 1101 16-17 0.6642224271889603 1101 18-19 0.6536912525402485 1101 20-21 0.7188311702233605 1101 22-23 0.6576071071513283 1101 24-25 0.701542484530755 1101 26-27 0.6732203118735356 1101 28-29 0.6650580437491485 1101 30-31 0.6566047154169254 1101 32-33 0.7037543113876765 1101 34-35 0.6431310185772432 1101 36-37 0.6105612351932379 1101 38-39 0.5875913645677215 1101 40-41 0.5795216840320592 1101 42-43 0.5628573257614207 1101 44-45 0.6554281089537426 1101 46-47 0.7049587935679327 1101 48-49 0.7299214181654037 1101 50-51 0.6786467928402118 1101 52-53 0.7919019934119333 1101 54-55 0.8679450612629296 1101 56-57 0.7895188086208051 1101 58-59 0.7353180986189543 1101 60-61 0.830658231052098 1101 62-63 0.7112375485446947 1101 64-65 0.8083654612486058 1101 66-67 0.774269030935578 1101 68-69 0.8153763661597679 1101 70-71 0.8298102128300684 1101 72-73 0.797888788630587 1101 74-75 0.8004555718170749 1101 76-77 0.7744324457842815 1101 78-79 0.8773105665921719 1101 80-81 0.6027552070541411 1101 82-83 0.7604287425082745 1101 84-85 0.8605568983243117 1101 86-87 0.9607472428490134 1101 88-89 0.8709289432828271 1101 90-91 0.901138077922905 1101 92-93 1.061276933721924 1101 94-95 1.1129433646250035 1101 96-97 1.0412693469460486 1101 98-99 1.082264403426219 1101 100-101 1.0924797880801798 1102 1 0.2635845687942293 1102 2 0.1898394190319408 1102 3 0.3054085259821626 1102 4 0.21917265492204052 1102 5 0.06628027319308671 1102 6 0.03512828583862415 1102 7 0.15539025372977733 1102 8 0.14523544402253918 1102 9 0.1503654026059138 1102 10-11 0.3493924937965289 1102 12-13 0.2693558732970942 1102 14-15 0.3444520049937836 1102 16-17 0.3603693202522109 1102 18-19 0.24886282237788038 1102 20-21 0.25890283509135514 1102 22-23 0.37042200153845073 1102 24-25 0.31197015433960473 1102 26-27 0.37564147685362315 1102 28-29 0.32265941442135215 1102 30-31 0.13865046414647253 1102 32-33 0.25488661934468126 1102 34-35 0.1471508262731973 1102 36-37 -0.025655054512299102 1102 38-39 0.22139296203483383 1102 40-41 0.07664705370790159 1102 42-43 0.11659574207792645 1102 44-45 0.13148200752893047 1102 46-47 0.04176410276512854 1102 48-49 0.23591741751314288 1102 50-51 0.21834399300600893 1102 52-53 0.10101129851747004 1102 54-55 0.131669797688879 1102 56-57 0.24655902855018752 1102 58-59 0.20089445408090256 1102 60-61 0.18671303138530249 1102 62-63 0.16411384546152163 1102 64-65 0.1806232766346696 1102 66-67 0.07154663451638754 1102 68-69 0.004022900663557039 1102 70-71 0.10517011732116543 1102 72-73 0.19666133275414666 1102 74-75 0.12926123618523633 1102 76-77 0.17224362180260755 1102 78-79 0.2241733130732797 1102 80-81 0.28817920136919284 1102 82-83 0.023590502546316117 1102 84-85 0.2665392191645637 1102 86-87 0.16326570641377103 1102 88-89 0.12115116716472585 1102 90-91 0.305407473431071 1102 92-93 0.2981930402129116 1102 94-95 0.1968717960182964 1102 96-97 0.2015423832330967 1102 98-99 0.46828135896664236 1102 100-101 0.4511153866932993 1103 1 0.293676662009247 1103 2 0.30631600261815706 1103 3 0.3286940628889603 1103 4 0.2609142282250758 1103 5 0.23675003967614572 1103 6 0.18164614597775142 1103 7 0.11332172794032402 1103 8 0.2972673914033237 1103 9 0.20360688242546843 1103 10-11 0.3604825111988319 1103 12-13 0.25714836873078184 1103 14-15 0.3957125937958992 1103 16-17 0.3041612979312305 1103 18-19 0.27492793482172573 1103 20-21 0.45508727081067235 1103 22-23 0.4161359037307548 1103 24-25 0.4414577210364783 1103 26-27 0.5206909272186877 1103 28-29 0.4625672732660817 1103 30-31 0.4765064804078065 1103 32-33 0.5885358789075639 1103 34-35 0.5610357600916842 1103 36-37 0.35565160486744674 1103 38-39 0.4482145715806922 1103 40-41 0.41468428410393443 1103 42-43 0.46602069881185315 1103 44-45 0.6597451803103667 1103 46-47 0.5495371344584328 1103 48-49 0.6731526075722556 1103 50-51 0.604593291879894 1103 52-53 0.6721320935585311 1103 54-55 0.5954236149310717 1103 56-57 0.7015426569557448 1103 58-59 0.7307856776598385 1103 60-61 0.6586857246204545 1103 62-63 0.8211066496304014 1103 64-65 0.7139369544666252 1103 66-67 0.6647463104403144 1103 68-69 0.6403961250766557 1103 70-71 0.4372100227829918 1103 72-73 0.3734745527567327 1103 74-75 0.4628126976436704 1103 76-77 0.5732676254844158 1103 78-79 0.5357978642327161 1103 80-81 0.47372138159575883 1103 82-83 0.5223910375594762 1103 84-85 0.3675138443916808 1103 86-87 0.3719270708890896 1103 88-89 0.4913941762781704 1103 90-91 0.5536363209954658 1103 92-93 0.5034285946178976 1103 94-95 0.5256373825911957 1103 96-97 0.6980566478536723 1103 98-99 0.8532079864181874 1103 100-101 1.020338227344709 1104 1 0.4411408626295916 1104 2 0.366657433059256 1104 3 0.3423225163845238 1104 4 0.4229378819430849 1104 5 0.4092112440310345 1104 6 0.3618171008995361 1104 7 0.3436516047818259 1104 8 0.3800084229815681 1104 9 0.41393129407878604 1104 10-11 0.5005552844942045 1104 12-13 0.553409029443273 1104 14-15 0.6689259806305472 1104 16-17 0.7258178252356018 1104 18-19 0.6288923535702011 1104 20-21 0.7487514877307078 1104 22-23 0.703201625015744 1104 24-25 0.7966508483226278 1104 26-27 0.5397241641921227 1104 28-29 0.5280857710104954 1104 30-31 0.6842392422933941 1104 32-33 0.7251708570707862 1104 34-35 0.632938939968291 1104 36-37 0.6192323578169763 1104 38-39 0.7662434603737012 1104 40-41 0.636816858063753 1104 42-43 0.6487109137279887 1104 44-45 0.6222535099283348 1104 46-47 0.6047024564011565 1104 48-49 0.5120340657228226 1104 50-51 0.49316865155159917 1104 52-53 0.4758728710534612 1104 54-55 0.5454756300993608 1104 56-57 0.5014501322784994 1104 58-59 0.422378396495084 1104 60-61 0.446033813961904 1104 62-63 0.5618620920469795 1104 64-65 0.621376322038877 1104 66-67 0.5209744676651482 1104 68-69 0.536322642265624 1104 70-71 0.5577356396541262 1104 72-73 0.6449817461908367 1104 74-75 0.59880719410328 1104 76-77 0.6513872172252739 1104 78-79 0.5979274658972002 1104 80-81 0.7368687547099064 1104 82-83 0.8032139798442124 1104 84-85 0.6384476724683665 1104 86-87 0.5674739013196408 1104 88-89 0.5231445226464544 1104 90-91 0.7452376690752196 1104 92-93 0.8495034868721909 1104 94-95 0.8659378720611208 1104 96-97 0.939637621328334 1104 98-99 0.9658819495904893 1104 100-101 0.9821231386312839 1105 1 0.1600443631329469 1105 2 0.28763860442715483 1105 3 0.26203646000974246 1105 4 0.2566368177630949 1105 5 0.26641902318633015 1105 6 0.2535088728251793 1105 7 0.20225812037163848 1105 8 0.24205330822982773 1105 9 0.32010067477688864 1105 10-11 0.3549651746136959 1105 12-13 0.29791219501562693 1105 14-15 0.41620456223439106 1105 16-17 0.20395471329881332 1105 18-19 0.24885699604342904 1105 20-21 0.40075118975531865 1105 22-23 0.32848154917352446 1105 24-25 0.42050914687482077 1105 26-27 0.38454245122482433 1105 28-29 0.2670541269396054 1105 30-31 -0.13887532466637964 1105 32-33 0.06910437360071597 1105 34-35 0.1810573876829693 1105 36-37 0.12533086531816906 1105 38-39 0.04129534596280138 1105 40-41 0.11370337313751833 1105 42-43 0.21831937989134076 1105 44-45 0.27834205023595615 1105 46-47 0.1487464235855782 1105 48-49 0.38687420567136854 1105 50-51 0.28172535614354643 1105 52-53 0.2665824062243374 1105 54-55 0.29643583082491176 1105 56-57 0.5217155800759983 1105 58-59 0.4823871443200929 1105 60-61 0.44546075235216165 1105 62-63 0.39328626043137405 1105 64-65 0.4106242795965258 1105 66-67 0.4341468942876645 1105 68-69 0.4618593829225759 1105 70-71 0.3539402405439773 1105 72-73 0.4620169493197466 1105 74-75 0.38717225390852406 1105 76-77 0.4848668331699564 1105 78-79 0.4405461035920055 1105 80-81 0.4924723359079941 1105 82-83 0.46302887719876296 1105 84-85 0.43385219278833276 1105 86-87 0.5665629123118521 1105 88-89 0.396682268762266 1105 90-91 0.41878814989346935 1105 92-93 0.3884993514232349 1105 94-95 0.4025441071545437 1105 96-97 0.46557001171105483 1105 98-99 0.5948747053759362 1105 100-101 0.4598571772840181 1106 1 0.18639922532067388 1106 2 0.2254164347313754 1106 3 0.32863877240204786 1106 4 0.21256075253031526 1106 5 0.26197500458127365 1106 6 0.3349863929372958 1106 7 0.27631238759889953 1106 8 0.42061630007608386 1106 9 0.42266737480253624 1106 10-11 0.5328638710808704 1106 12-13 0.5476579928179817 1106 14-15 0.5489125307258433 1106 16-17 0.6561049044129987 1106 18-19 0.6078061866976796 1106 20-21 0.5039220376911118 1106 22-23 0.38872870110292723 1106 24-25 0.39663137553623073 1106 26-27 0.23496420809089358 1106 28-29 0.3908244629395412 1106 30-31 0.45492672839536397 1106 32-33 0.6214165618978953 1106 34-35 0.586979570787058 1106 36-37 0.4179084847474783 1106 38-39 0.3855316215414817 1106 40-41 0.382829670300346 1106 42-43 0.3747550676146858 1106 44-45 0.34856045659957147 1106 46-47 0.21540255213429305 1106 48-49 0.38827490304434065 1106 50-51 0.4267942978055643 1106 52-53 0.5233111492998219 1106 54-55 0.5314405743849449 1106 56-57 0.504244041787949 1106 58-59 0.7016742638556934 1106 60-61 0.6363499654980487 1106 62-63 0.6101114139199737 1106 64-65 0.7291244732643491 1106 66-67 0.7316412703234505 1106 68-69 0.7346209086901112 1106 70-71 0.516097183651226 1106 72-73 0.5979407321354628 1106 74-75 0.5570305352160574 1106 76-77 0.5916389316666049 1106 78-79 0.5785208095764034 1106 80-81 0.6749625002139972 1106 82-83 0.7163797946973993 1106 84-85 0.7979830703040278 1106 86-87 0.8161782147283994 1106 88-89 0.7888408115811458 1106 90-91 0.8542954308325186 1106 92-93 0.9427128328698835 1106 94-95 0.8205783724992912 1106 96-97 0.7107691532976332 1106 98-99 0.7368360981577595 1106 100-101 0.8962432444007149 1107 1 0.2607231233792646 1107 2 0.24565410591085168 1107 3 0.3493801779932433 1107 4 0.35794481248366594 1107 5 0.3273245235335054 1107 6 0.31115081559595126 1107 7 0.2906885847773779 1107 8 0.38118795942985173 1107 9 0.37909524353350577 1107 10-11 0.5505517397732689 1107 12-13 0.47363141790068397 1107 14-15 0.5268517321724957 1107 16-17 0.5385025479773091 1107 18-19 0.48290546659301725 1107 20-21 0.6052196255787905 1107 22-23 0.5960661017782698 1107 24-25 0.7245445018053758 1107 26-27 0.5518037175083919 1107 28-29 0.6095177893745998 1107 30-31 0.5590305315695119 1107 32-33 0.6270835395933503 1107 34-35 0.6442264315037391 1107 36-37 0.5615915284989512 1107 38-39 0.38415554640530303 1107 40-41 0.6377714880804461 1107 42-43 0.6462577223790547 1107 44-45 0.6859594789939223 1107 46-47 0.646711677564852 1107 48-49 0.8379320413204709 1107 50-51 0.6646286365415435 1107 52-53 0.7387560981674 1107 54-55 0.852700260287186 1107 56-57 0.8179448188640563 1107 58-59 0.807839906937339 1107 60-61 0.9079445652227207 1107 62-63 0.9025018286130972 1107 64-65 1.0346382231338325 1107 66-67 0.8929079784789877 1107 68-69 0.9984717454512335 1107 70-71 0.87470580777169 1107 72-73 0.8513285079049169 1107 74-75 1.0389166893105752 1107 76-77 1.1194780060845808 1107 78-79 1.1721404767342705 1107 80-81 1.019406848236759 1107 82-83 1.044170052195632 1107 84-85 1.0178232637644875 1107 86-87 0.8906497757045067 1107 88-89 0.977144434639591 1107 90-91 1.081561402326411 1107 92-93 1.2814662355221422 1107 94-95 1.125464003988295 1107 96-97 0.9490995815834573 1107 98-99 0.9899518270409295 1107 100-101 0.8563240485244776 1108 1 0.2840007504782527 1108 2 0.29991357886992986 1108 3 0.30885779218857223 1108 4 0.17522575043258826 1108 5 0.2362104070134734 1108 6 0.12891809077625282 1108 7 0.1812279985103018 1108 8 0.14059395015718934 1108 9 0.3147043574360566 1108 10-11 0.28711580602620046 1108 12-13 0.36228448835873195 1108 14-15 0.4330541856642114 1108 16-17 0.4078064609707184 1108 18-19 0.37511071805509033 1108 20-21 0.34595696345616744 1108 22-23 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-2.8772774553387315 2316 88-89 -2.8173840513032857 2316 90-91 -2.631654537263639 2316 92-93 -2.5814153246459632 2316 94-95 -3.1305746812459354 2316 96-97 -3.311439231034793 2316 98-99 -3.2756304922313575 2316 100-101 -3.0896951209549535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7759.0 3 2811.0 4 2915.0 5 2900.0 6 3010.0 7 3541.0 8 4293.0 9 5134.0 10 6124.0 11 7433.0 12 9096.0 13 10573.0 14 11572.0 15 14018.0 16 17482.0 17 21779.0 18 19771.0 19 14138.0 20 14063.0 21 14738.0 22 16146.0 23 18074.0 24 20609.0 25 23544.0 26 27562.0 27 32356.0 28 38501.0 29 46561.0 30 56785.0 31 71062.0 32 90419.0 33 118476.0 34 160559.0 35 228256.0 36 343385.0 37 510035.0 38 464396.0 39 83492.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18725601061999 19.00522149564875 14.77221102315748 23.035311470573774 2 14.949583947103953 20.17169188288765 41.10197475165644 23.776749418351955 3 18.758595574132244 24.840044157211942 29.971176682726814 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26.48801259819727 29.372595459897916 21.838474686795255 24-25 22.125649838376052 26.706941571566766 29.356492878321944 21.810915711735234 26-27 22.012795633598174 26.91968911407736 29.150097028967124 21.91741822335734 28-29 21.771267847118654 26.875806763987924 29.431910348305756 21.921015040587662 30-31 22.011243876994488 26.616420370158465 29.456468611007235 21.91586714183981 32-33 21.784514505203834 26.79490801551683 29.32515221631556 22.095425262963772 34-35 22.18561821752216 26.790743232562658 29.112701100295936 21.91093744961925 36-37 22.16682951571903 26.701648084836165 29.077481416531175 22.054040982913627 38-39 21.976541180726493 26.781293498690616 29.276889563459918 21.96527575712297 40-41 22.163632431582258 26.652249134948097 29.237043881723814 21.947074551745832 42-43 22.001975009815087 26.744082490376105 29.041923643045774 22.212018856763034 44-45 22.150982602823614 26.904670865160334 28.798514570712708 22.145831961303347 46-47 22.206191205360994 27.051800324259478 28.48718030883484 22.254828161544683 48-49 22.32746489810217 27.182065919341227 28.22964785850016 22.260821324056444 50-51 22.204952092756976 27.25003617421502 28.18299670342806 22.362015029599945 52-53 22.42745661001128 27.26671675963749 28.07311922323653 22.232707407114695 54-55 22.27367365596154 27.171429414019876 28.144327464684892 22.410569465333687 56-57 22.218936289990683 27.54468305250083 28.039762052188777 22.19661860531971 58-59 22.394096958374874 27.21281978134713 28.080263026738155 22.31282023353984 60-61 22.343306570113793 27.09674222542335 28.385136316239755 22.1748148882231 62-63 22.258876037770165 27.210817878402093 28.350666778083077 22.179639305744665 64-65 22.173354509196677 27.22711270720882 28.21124771788654 22.38828506570796 66-67 22.10640604191994 27.48250910894815 28.019323101582344 22.391761747549566 68-69 21.992277132829027 27.338878994583926 28.22810794080407 22.440735931782978 70-71 22.064920141666253 27.217487429597487 28.17836718867362 22.53922524006264 72-73 22.134875027379973 27.25116470572968 28.07830534775614 22.535654919134206 74-75 22.018295228269576 27.395416302663474 27.967832833609297 22.618455635457657 76-77 22.169817257102494 27.328274926869017 27.982776562533186 22.519131253495303 78-79 22.157866138252786 27.332773112879437 27.932469901589073 22.5768908472787 80-81 22.000755142936367 27.554851105084825 27.9282928853343 22.51610086664451 82-83 22.13496657447074 27.4616164406998 27.923861177125314 22.479555807704145 84-85 22.16382237795514 27.41208070583192 27.71658575240432 22.707511163808615 86-87 22.179064762800678 27.28474537077093 27.93952703287299 22.596662833555403 88-89 22.250696363494075 27.258496657101293 27.79584584716458 22.694961132240046 90-91 22.209804333707638 27.352166883313377 27.876189107313955 22.561839675665027 92-93 22.185788932473802 27.28974671653006 27.83732765556107 22.68713669543507 94-95 22.178401023288462 27.158909580351448 27.798936794633438 22.863752601726652 96-97 22.129360658062183 27.20581395508911 27.777628096220376 22.887197290628333 98-99 22.049589748451698 27.17213806419177 27.739747448541035 23.038524738815497 100-101 22.055715125130153 27.201278182783522 27.789518452662325 22.953488239424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 448.0 1 319.5 2 297.0 3 409.5 4 360.0 5 373.5 6 672.0 7 1483.0 8 2702.5 9 3197.0 10 2701.0 11 2310.0 12 2401.0 13 2604.0 14 2816.5 15 3044.0 16 3136.5 17 3251.5 18 3536.5 19 3794.0 20 4183.5 21 4787.5 22 5850.0 23 7291.0 24 9063.0 25 11516.0 26 14485.5 27 17839.5 28 21102.5 29 24401.0 30 28868.0 31 33747.5 32 38398.0 33 43883.5 34 50492.0 35 55770.0 36 61264.5 37 67318.5 38 71874.5 39 75044.0 40 77990.0 41 82518.5 42 85299.0 43 88802.5 44 93406.0 45 96450.5 46 98059.5 47 99909.0 48 103732.0 49 103793.5 50 100694.0 51 96798.0 52 91040.0 53 86824.5 54 82725.5 55 75801.0 56 68640.5 57 60922.5 58 53722.0 59 47835.5 60 40610.5 61 32100.0 62 24341.0 63 18490.0 64 13972.0 65 10426.5 66 7415.5 67 5378.5 68 3574.0 69 1975.5 70 1215.0 71 735.5 72 436.5 73 287.5 74 212.0 75 131.5 76 78.0 77 56.0 78 43.0 79 22.0 80 19.0 81 21.0 82 18.5 83 15.5 84 11.0 85 8.5 86 6.5 87 2.0 88 4.0 89 6.0 90 3.5 91 1.5 92 2.0 93 2.5 94 1.5 95 2.0 96 1.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04053619437871893 2 0.04171571506869136 3 0.026460580811714682 4 0.04525427713860862 5 0.06593520656945843 6 0.0652668115118074 7 0.06503090737381291 8 0.06801902645507638 9 0.06621042806378533 10-11 0.07350379766344815 12-13 0.07389697122677229 14-15 0.06797970909874397 16-17 0.05712811875099768 18-19 0.04922533012818245 20-21 0.05708880139466526 22-23 0.03224023219257956 24-25 0.018321888050904966 26-27 0.013800392072677345 28-29 0.01783042109674979 30-31 0.013092679658693892 32-33 0.009141285347286277 34-35 0.010694320922416633 36-37 0.005936920806194529 38-39 0.008885722531125585 40-41 0.008099375404477303 42-43 0.005012962932382797 44-45 0.001317131437135872 46-47 0.002850508334100022 48-49 9.829339083103524E-4 50-51 0.006212142300521427 52-53 0.005307843104875903 54-55 0.009082309312787655 56-57 0.022784407994633965 58-59 0.009534458910610418 60-61 0.021644204660993958 62-63 0.015471379716804946 64-65 0.01018319529009525 66-67 0.017279978108095992 68-69 0.027423856041858832 70-71 0.02075956414351464 72-73 0.01922618724655049 74-75 0.01975697155703808 76-77 0.02783668828334918 78-79 0.037528416619289254 80-81 0.03306589667556026 82-83 0.03198466937641887 84-85 0.023236557592456727 86-87 0.025556281616069165 88-89 0.02174249805182499 90-91 0.018852672361392556 92-93 0.03379326776770992 94-95 0.02451437167326019 96-97 0.03819681167694029 98-99 0.056184502199019735 100-101 0.06575827846596256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2543406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.474047005800344 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62028883527576 41.949634574402864 2 13.530697882942382 15.01205140759258 3 4.621898423636547 7.691862312265449 4 2.1067724270733437 4.67484770599963 5 1.1422823845444925 3.168351334705944 6 0.660322601908807 2.1978460234368926 7 0.4393257200290444 1.70598229506238 8 0.31220770864742453 1.3855540084064355 9 0.24520528049988044 1.2242276330868742 >10 1.1893205188019784 12.01958666611727 >50 0.08809125378485087 3.364452640245869 >100 0.04152265076197086 4.029669291470356 >500 0.0017083962151071593 0.5847805806655109 >1k 2.1354952688586884E-4 0.3108115301757604 >5k 7.118317562862295E-5 0.28107692437950227 >10k+ 7.118317562862295E-5 0.39926507198667427 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10111 0.3975377898770389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7118 0.2798609423741235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4862 0.19116098648819732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.931735633241409E-5 0.0 22-23 0.0 0.0 0.0 1.1795206899724228E-4 0.0 24-25 0.0 0.0 0.0 1.1795206899724228E-4 0.0 26-27 0.0 0.0 0.0 1.5726942532965637E-4 0.0 28-29 0.0 0.0 0.0 6.880537358172466E-4 0.0 30-31 0.0 0.0 0.0 0.0018675744257896695 0.0 32-33 0.0 0.0 0.0 0.003695831495246925 0.0 34-35 0.0 0.0 0.0 0.005818968737197286 0.0 36-37 0.0 0.0 0.0 0.007745519197485577 0.0 38-39 0.0 0.0 0.0 0.009750704370438696 0.0 40-41 0.0 0.0 0.0 0.011539644083563536 0.0 42-43 0.0 0.0 0.0 0.014272200348666317 0.0 44-45 0.0 0.0 0.0 0.01651328965961392 0.0 46-47 0.0 0.0 0.0 0.02131000713216844 0.0 48-49 0.0 0.0 0.0 0.02722726926019676 0.0 50-51 0.0 0.0 0.0 0.03222057351441335 0.0 52-53 0.0 0.0 0.0 0.043681582885312056 0.0 54-55 0.0 0.0 0.0 0.0633402610515191 0.0 56-57 0.0 0.0 0.0 0.09609161887642004 0.0 58-59 0.0 0.0 0.0 0.13711928020929415 0.0 60-61 0.0 0.0 0.0 0.17726230102468893 0.0 62-63 0.0 0.0 0.0 0.22393200299126448 0.0 64-65 0.0 0.0 0.0 0.2812173911675918 0.0 66-67 0.0 0.0 0.0 0.36075640302806555 0.0 68-69 0.0 0.0 0.0 0.4613105418482146 0.0 70-71 0.0 0.0 0.0 0.5569106937704794 0.0 72-73 0.0 0.0 0.0 0.6545356895438637 0.0 74-75 0.0 0.0 0.0 0.7588839532500906 0.0 76-77 0.0 0.0 0.0 0.8763838726495101 0.0 78-79 0.0 0.0 0.0 0.9816560942295489 0.0 80-81 0.0 0.0 0.0 1.0999227020774505 0.0 82-83 0.0 0.0 0.0 1.2314196003312095 0.0 84-85 0.0 0.0 0.0 1.4080528236545797 0.0 86-87 0.0 0.0 0.0 1.615727099802391 0.0 88-89 0.0 0.0 0.0 1.8609101338913252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4875 0.0 47.669346 1 GTATCAA 8950 0.0 43.434544 1 ATCAACG 10880 0.0 35.16187 3 TCAACGC 11040 0.0 34.696007 4 TATCAAC 11225 0.0 34.251194 2 CAACGCA 11205 0.0 34.061203 5 AACGCAG 11575 0.0 33.01088 6 ACGCAGA 13385 0.0 28.298407 7 CGCAGAG 13460 0.0 27.824594 8 GTACATG 9385 0.0 24.862923 1 GCAGAGT 14415 0.0 24.827217 9 GTGGTAT 2555 0.0 23.994062 1 TACATGG 9570 0.0 23.389584 2 TGGTATC 2600 0.0 22.66532 2 ACATGGG 9420 0.0 22.550781 3 GAGTACT 8070 0.0 21.877127 12-13 AGAGTAC 12800 0.0 20.940289 10-11 CAGAGTA 13680 0.0 20.531227 10-11 GTACTTT 8700 0.0 20.237698 14-15 CATGGGG 5020 0.0 19.785912 4 >>END_MODULE