FastQCFastQC Report
Thu 26 May 2016
SRR936790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543406
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.3978130113713658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.3150499762916341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.21864381856455478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.050.432451
GTATCAA96400.040.0691951
ATCAACG122700.031.1598013
TCAACGC123450.030.9686684
CAACGCA125400.030.4870995
TATCAAC126500.030.3387972
AACGCAG129850.029.4411376
ACGCAGA149100.025.4175977
CGCAGAG149200.025.1140868
GCAGAGT159700.022.7194429
GTACATG96000.022.171951
GAGTACT94100.021.75252312-13
ACATGGG95200.020.8037913
TACATGG99900.020.634872
AGAGTAC141950.020.62561610-11
AGTACTT98650.020.38817412-13
GTACTTT99450.020.36823314-15
CAGAGTA152400.020.16176410-11
GCACCGT5350.018.640876
TACTTTT114850.017.2028914-15
Produced by FastQC (version 0.11.5)