##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936790_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543406 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.082822797461354 31.0 31.0 34.0 30.0 34.0 2 32.160681385512184 33.0 31.0 34.0 30.0 34.0 3 32.30127710636839 34.0 31.0 34.0 30.0 34.0 4 36.00068648104156 37.0 35.0 37.0 35.0 37.0 5 35.90010914498118 37.0 35.0 37.0 35.0 37.0 6 35.92901565853033 37.0 35.0 37.0 35.0 37.0 7 35.876067367931036 37.0 35.0 37.0 35.0 37.0 8 35.85601512302794 37.0 35.0 37.0 35.0 37.0 9 37.5284461073065 39.0 37.0 39.0 35.0 39.0 10-11 37.418780367743096 39.0 37.0 39.0 34.0 39.0 12-13 37.33942536111026 39.0 37.0 39.0 34.0 39.0 14-15 38.53843959635229 40.0 38.0 41.0 34.0 41.0 16-17 38.4529982629592 40.0 38.0 41.0 34.0 41.0 18-19 38.47454220836154 40.0 38.0 41.0 33.5 41.0 20-21 38.57078146391099 40.0 38.0 41.0 34.0 41.0 22-23 38.50909135230474 40.0 38.0 41.0 34.0 41.0 24-25 38.42400485805255 40.0 38.0 41.0 34.0 41.0 26-27 38.25868304155923 40.0 38.0 41.0 34.0 41.0 28-29 38.09899795785651 40.0 37.5 41.0 33.0 41.0 30-31 38.06907921896858 40.0 37.0 41.0 33.0 41.0 32-33 38.01094202026731 40.0 37.0 41.0 33.0 41.0 34-35 37.902946482000914 40.0 37.0 41.0 33.0 41.0 36-37 37.74291088406648 40.0 37.0 41.0 33.0 41.0 38-39 37.61631921918875 40.0 37.0 41.0 33.0 41.0 40-41 37.39602446483181 40.0 37.0 41.0 32.0 41.0 42-43 37.187153761530794 40.0 36.0 41.0 31.5 41.0 44-45 36.970927960380685 39.0 36.0 41.0 31.0 41.0 46-47 36.65833571203339 39.0 36.0 41.0 30.5 41.0 48-49 36.32778565435483 39.0 35.5 41.0 30.0 41.0 50-51 36.10202165914525 39.0 35.0 40.0 29.5 41.0 52-53 35.88176936753314 39.0 35.0 40.0 29.0 41.0 54-55 35.60878424443443 38.5 35.0 40.0 28.0 41.0 56-57 35.35765446020022 38.0 34.5 40.0 28.0 41.0 58-59 35.00322952764914 38.0 34.0 40.0 27.0 41.0 60-61 34.66756841023415 38.0 34.0 40.0 26.0 41.0 62-63 34.327143601925926 37.0 33.5 40.0 26.0 41.0 64-65 33.912203556962595 36.5 33.0 39.0 26.0 40.5 66-67 33.471684819490086 36.0 32.5 39.0 25.0 40.0 68-69 33.02685237826756 35.5 32.0 39.0 23.5 40.0 70-71 32.625060253848574 35.0 32.0 38.0 23.0 40.0 72-73 32.211769571983396 35.0 31.0 37.5 22.0 39.5 74-75 31.716370292434632 35.0 31.0 37.0 20.5 39.0 76-77 30.267887038089867 33.5 29.5 35.5 20.0 37.5 78-79 30.98354509661454 34.0 30.5 36.0 20.0 38.0 80-81 30.708156110349666 34.0 30.0 35.5 18.0 37.0 82-83 30.471202198941107 34.0 30.0 35.0 18.0 37.0 84-85 30.108949770504594 34.0 30.0 35.0 16.0 36.5 86-87 29.705057116323545 34.0 30.0 35.0 9.5 36.0 88-89 29.30609584156049 34.0 29.0 35.0 7.0 36.0 90-91 28.823741471082478 34.0 29.0 35.0 2.0 35.0 92-93 28.354684820276432 33.0 29.0 35.0 2.0 35.0 94-95 27.821902401740026 33.0 27.0 35.0 2.0 35.0 96-97 27.124402474477137 33.0 26.5 34.5 2.0 35.0 98-99 26.258543661531036 32.5 25.0 34.0 2.0 35.0 100-101 22.91153712777276 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28423657225255994 1101 2 0.23961029143357138 1101 3 0.2780605950904729 1101 4 0.20640014269014273 1101 5 0.32541556286026463 1101 6 0.28408639600333174 1101 7 0.19683168052318223 1101 8 0.2476701646102839 1101 9 0.3578687110765344 1101 10-11 0.3157737062689563 1101 12-13 0.3417282575948519 1101 14-15 0.3951379005933333 1101 16-17 0.4413127700386923 1101 18-19 0.43683628045331346 1101 20-21 0.3799944433043052 1101 22-23 0.4011410481557647 1101 24-25 0.43258972758427916 1101 26-27 0.4489961322271441 1101 28-29 0.3032724840148333 1101 30-31 0.42749625028450566 1101 32-33 0.4763986941000127 1101 34-35 0.4825849949186747 1101 36-37 0.587507826310798 1101 38-39 0.6219840243163617 1101 40-41 0.7788548865340985 1101 42-43 0.7770635081868207 1101 44-45 0.6987856220983986 1101 46-47 0.7659444273876304 1101 48-49 0.8191484135791853 1101 50-51 0.6711374308803002 1101 52-53 0.7161756618678226 1101 54-55 0.6539013446122723 1101 56-57 0.7534156383532391 1101 58-59 0.8715001154614583 1101 60-61 0.7255071499143426 1101 62-63 0.615659470836917 1101 64-65 0.6029614177670055 1101 66-67 0.6970710058731768 1101 68-69 0.672139589004118 1101 70-71 0.7297726222290635 1101 72-73 0.7771005023713968 1101 74-75 0.7102399181540839 1101 76-77 0.6156433753371822 1101 78-79 0.9294171974543453 1101 80-81 1.1497174182170653 1101 82-83 1.0003990856294607 1101 84-85 0.8886740162451048 1101 86-87 0.8675882133629038 1101 88-89 0.7765374232273423 1101 90-91 0.650668738025626 1101 92-93 0.8983586455490133 1101 94-95 0.9558142093311481 1101 96-97 1.0524281672291274 1101 98-99 1.1580852325774735 1101 100-101 1.2074195475420808 1102 1 0.24725149233702126 1102 2 0.174849681219996 1102 3 0.2216689079164169 1102 4 0.15368948157288997 1102 5 0.17894737700792973 1102 6 0.18878080721965063 1102 7 0.15897167403107915 1102 8 0.14811801471265085 1102 9 0.2766268132410161 1102 10-11 0.2326371484314791 1102 12-13 0.25691224458519457 1102 14-15 0.33167806250316545 1102 16-17 0.38427920264935267 1102 18-19 0.38143818678638297 1102 20-21 0.17768745029528787 1102 22-23 0.30385940602673855 1102 24-25 0.2168338157789762 1102 26-27 0.31156433632209257 1102 28-29 0.28315491247132485 1102 30-31 0.35220076893589436 1102 32-33 0.3431517746340518 1102 34-35 0.312785479793412 1102 36-37 0.33088401942864465 1102 38-39 0.41522231899234896 1102 40-41 0.4599855721564907 1102 42-43 0.36455824044006135 1102 44-45 0.3130225280393617 1102 46-47 0.4142404020753432 1102 48-49 0.6179125348401939 1102 50-51 0.5295910182106525 1102 52-53 0.4123973787182891 1102 54-55 0.34850107261881647 1102 56-57 0.5143100025177105 1102 58-59 0.518485014195555 1102 60-61 0.5037687927929966 1102 62-63 0.44779590018769966 1102 64-65 0.5478061902190206 1102 66-67 0.43792642570861773 1102 68-69 0.42563199964536125 1102 70-71 0.24625978787495484 1102 72-73 0.2733402267211176 1102 74-75 0.3580437985073921 1102 76-77 0.3952075883230215 1102 78-79 0.44566896552937507 1102 80-81 0.42010015779391097 1102 82-83 0.435755620975538 1102 84-85 0.34684454560334643 1102 86-87 0.30323740514844033 1102 88-89 0.09635337607369365 1102 90-91 0.33002827130222556 1102 92-93 0.3673047684113335 1102 94-95 0.6041856993382986 1102 96-97 0.5635603839038765 1102 98-99 0.607887807041319 1102 100-101 0.9397802546254894 1103 1 0.1995519051025525 1103 2 0.17466271425562496 1103 3 0.18425825655228323 1103 4 0.13847228828260683 1103 5 0.1500135201671 1103 6 0.22311465351363324 1103 7 0.19138982804391702 1103 8 0.1840722413645537 1103 9 0.20469438981358223 1103 10-11 0.21336516902661629 1103 12-13 0.23415932382563653 1103 14-15 0.37584541231834834 1103 16-17 0.420133713586921 1103 18-19 0.3815681048565054 1103 20-21 0.3325334901911603 1103 22-23 0.2282566772678578 1103 24-25 0.21604471930881886 1103 26-27 0.27575057459471 1103 28-29 0.29737303100838375 1103 30-31 0.23727025832432247 1103 32-33 0.2575386428415243 1103 34-35 0.3212709035612349 1103 36-37 0.3950213586490392 1103 38-39 0.23994270750011282 1103 40-41 0.4495019988822264 1103 42-43 0.370130335380928 1103 44-45 0.5077203366906602 1103 46-47 0.5628839327786679 1103 48-49 0.5138251493574586 1103 50-51 0.576094016873121 1103 52-53 0.5688350348237208 1103 54-55 0.577625252241333 1103 56-57 0.5173844105755023 1103 58-59 0.6523974657159783 1103 60-61 0.5843668953180057 1103 62-63 0.6165100948155171 1103 64-65 0.5357554502613056 1103 66-67 0.3847928114454433 1103 68-69 0.43933332231678435 1103 70-71 0.33780840168271453 1103 72-73 0.37623917347389124 1103 74-75 0.28210836443242115 1103 76-77 0.37579945861588726 1103 78-79 0.42253108458418254 1103 80-81 0.7323532055951318 1103 82-83 0.6826709057645779 1103 84-85 0.4123361968092816 1103 86-87 0.41847425394010784 1103 88-89 0.2833826627707552 1103 90-91 0.3292866356768691 1103 92-93 0.434311810833659 1103 94-95 0.6978494368558579 1103 96-97 0.9823083764060208 1103 98-99 1.1536640315550848 1103 100-101 1.2749621638937754 1104 1 0.16382587402768678 1104 2 0.13202945235325814 1104 3 0.12421597325418077 1104 4 0.16587109840566683 1104 5 -0.020388171164825053 1104 6 0.1275035831517357 1104 7 0.10839950717983982 1104 8 0.11563370315857213 1104 9 0.13192397086375962 1104 10-11 0.09937764307895236 1104 12-13 0.15133823203444763 1104 14-15 0.22776517952051023 1104 16-17 0.1797756958202683 1104 18-19 0.08169658471920371 1104 20-21 0.18137884933552328 1104 22-23 0.18776490621130648 1104 24-25 0.19837682057779915 1104 26-27 0.2824022839042257 1104 28-29 0.21301833070683784 1104 30-31 0.2737585393308777 1104 32-33 0.34019865540187055 1104 34-35 0.2688578312146035 1104 36-37 0.31722584297977363 1104 38-39 0.3640964422850743 1104 40-41 0.36530118677443824 1104 42-43 0.42472804479591275 1104 44-45 0.4916862415867982 1104 46-47 0.46831939801479905 1104 48-49 0.544453693939488 1104 50-51 0.45059507874959337 1104 52-53 0.6499389069574946 1104 54-55 0.4924656351880259 1104 56-57 0.3821579168772473 1104 58-59 0.36935249297000894 1104 60-61 0.512259010585538 1104 62-63 0.5105496838717158 1104 64-65 0.6203421813596606 1104 66-67 0.5348256505148186 1104 68-69 0.48137212515292305 1104 70-71 0.4788179274098425 1104 72-73 0.7067104740448542 1104 74-75 0.5383686416229274 1104 76-77 0.6059864735205132 1104 78-79 0.8201613981613427 1104 80-81 0.7807498440249923 1104 82-83 0.7840391204218342 1104 84-85 0.7023262731780946 1104 86-87 0.7417756111285065 1104 88-89 0.7866449965978752 1104 90-91 0.6928927969574481 1104 92-93 0.650804214701477 1104 94-95 0.707914012368942 1104 96-97 1.0166796160928762 1104 98-99 1.2620037169711829 1104 100-101 1.4021279844150811 1105 1 0.17331530817824614 1105 2 0.18477650662098455 1105 3 0.16252245758987272 1105 4 0.1006348800795962 1105 5 0.07429059596616128 1105 6 0.1392673685711614 1105 7 0.17109419518587288 1105 8 0.14385105706219292 1105 9 0.10533840677262418 1105 10-11 0.11165622676364251 1105 12-13 0.14362728647446232 1105 14-15 0.32801371430144144 1105 16-17 0.23390983385361608 1105 18-19 0.24676288216273434 1105 20-21 0.3119786330120462 1105 22-23 0.07333465721458055 1105 24-25 0.13882668850728663 1105 26-27 0.19050704805628982 1105 28-29 0.16035617445536587 1105 30-31 0.19145532273647348 1105 32-33 0.11668173843170848 1105 34-35 0.1013363580509079 1105 36-37 0.1414494943186 1105 38-39 0.21150807239773428 1105 40-41 0.364448044944389 1105 42-43 0.32069197645633807 1105 44-45 0.410249900325077 1105 46-47 0.4475127253636586 1105 48-49 0.4384755834780165 1105 50-51 0.3898108041927415 1105 52-53 0.47772661031216757 1105 54-55 0.35772166305122255 1105 56-57 0.21964917217349011 1105 58-59 0.4170707963580682 1105 60-61 0.5039881126683312 1105 62-63 0.3435619445602356 1105 64-65 0.3624064017149635 1105 66-67 0.3245060053219433 1105 68-69 0.4642695446277827 1105 70-71 0.3677159719671508 1105 72-73 0.21213895308440556 1105 74-75 0.23279529493897044 1105 76-77 0.1546257539182676 1105 78-79 0.1536166683513578 1105 80-81 0.5394981892419679 1105 82-83 0.4703817346897772 1105 84-85 0.16611227524311545 1105 86-87 0.09448233883851032 1105 88-89 0.11281089814197642 1105 90-91 0.2254237191871198 1105 92-93 0.48318577468587876 1105 94-95 0.3614558202721945 1105 96-97 0.4842464930485981 1105 98-99 0.6422629722074937 1105 100-101 0.8987412194331803 1106 1 0.15929848574018735 1106 2 0.15920152143977617 1106 3 0.13424245430884696 1106 4 0.08345380834033733 1106 5 0.0878868785571143 1106 6 0.18097552292181263 1106 7 0.11554072661851933 1106 8 0.13041657468005496 1106 9 0.16028195890064012 1106 10-11 0.16022864945162496 1106 12-13 0.13250387385762252 1106 14-15 0.23664084515743156 1106 16-17 0.22162905730829152 1106 18-19 0.2845323973431775 1106 20-21 0.1945292683873987 1106 22-23 0.2413500341436432 1106 24-25 0.16407638492083976 1106 26-27 0.20267822254712087 1106 28-29 0.306342523301403 1106 30-31 0.2429310039540482 1106 32-33 0.3150552664543156 1106 34-35 0.3709229416338715 1106 36-37 0.4397599061901829 1106 38-39 0.4443610842242123 1106 40-41 0.34257040557848484 1106 42-43 0.23891343760789852 1106 44-45 0.20335259660595995 1106 46-47 0.20106673729832636 1106 48-49 0.3735260250448533 1106 50-51 0.1707733494141408 1106 52-53 0.19872086274666145 1106 54-55 0.3721842098906265 1106 56-57 0.3503224678650696 1106 58-59 0.339043771220922 1106 60-61 0.3506440048480428 1106 62-63 0.3326710318086725 1106 64-65 0.31169568374552625 1106 66-67 0.3819170809823902 1106 68-69 0.3977706436732831 1106 70-71 0.40718115333604743 1106 72-73 0.35163837981923507 1106 74-75 0.3582163448084721 1106 76-77 0.20056174772539848 1106 78-79 0.3566827541693449 1106 80-81 0.7009429300387389 1106 82-83 0.5925346313848436 1106 84-85 0.36211437498774046 1106 86-87 0.6199033324296757 1106 88-89 0.42925890237861 1106 90-91 0.5075179513473458 1106 92-93 0.7725888036702067 1106 94-95 0.8747666310164597 1106 96-97 0.9965438013543952 1106 98-99 1.0198507730908126 1106 100-101 0.7431171356268607 1107 1 0.1670967958262537 1107 2 0.11381569834711058 1107 3 0.11106750322907288 1107 4 0.15910557069450704 1107 5 0.12351772016136664 1107 6 0.0979112749516915 1107 7 0.1309231529130983 1107 8 0.16874584590570407 1107 9 0.20408715514768971 1107 10-11 0.20124436646115385 1107 12-13 0.23014287879689022 1107 14-15 0.19362279419222972 1107 16-17 0.2869741684122431 1107 18-19 0.26678846633041076 1107 20-21 0.2457051445643259 1107 22-23 0.2130821587347853 1107 24-25 0.25778145413921294 1107 26-27 0.2704932438879979 1107 28-29 0.3227359997961372 1107 30-31 0.23935885615559016 1107 32-33 0.19963885840437 1107 34-35 0.3126589699256428 1107 36-37 0.24403835657805217 1107 38-39 0.2774084584001173 1107 40-41 0.3863831089421481 1107 42-43 0.2192925208250429 1107 44-45 0.26431561778925783 1107 46-47 0.3796995412215267 1107 48-49 0.3383513957380089 1107 50-51 0.32416343930394476 1107 52-53 0.3558065201868814 1107 54-55 0.33733459052210435 1107 56-57 0.20357548744775755 1107 58-59 0.24146812910073834 1107 60-61 0.25379751725572675 1107 62-63 0.38732173875866494 1107 64-65 0.3559842281059744 1107 66-67 0.35407421856979937 1107 68-69 0.3464160430229626 1107 70-71 0.16824781405211553 1107 72-73 0.13792516434625668 1107 74-75 0.26285337365424866 1107 76-77 0.2268522899430785 1107 78-79 0.3667035840073929 1107 80-81 0.592661450908107 1107 82-83 0.5731746729949307 1107 84-85 0.5578978410937268 1107 86-87 0.4827005143820706 1107 88-89 0.18916333747290537 1107 90-91 0.18508157657114666 1107 92-93 0.34737793634065284 1107 94-95 0.38031273724725523 1107 96-97 0.46171994034927977 1107 98-99 0.6550521525269346 1107 100-101 0.828568302315432 1108 1 0.10774241973035714 1108 2 0.0963912684560313 1108 3 0.054475571608662676 1108 4 0.11811889821302657 1108 5 0.018547280154798784 1108 6 0.09542364572114792 1108 7 0.05507128690533136 1108 8 0.13922260339615633 1108 9 0.12079077691043949 1108 10-11 0.11457711177970253 1108 12-13 0.13334809024129157 1108 14-15 0.22611244880705073 1108 16-17 0.24923791101203108 1108 18-19 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-0.9140359418881445 2316 82-83 -1.03824872505297 2316 84-85 -1.3107043800071665 2316 86-87 -1.0321316626180952 2316 88-89 -1.0590317892821837 2316 90-91 -1.263389263236693 2316 92-93 -1.1070401539913206 2316 94-95 -1.1843320385408909 2316 96-97 -1.3014346021765029 2316 98-99 -1.343790642810088 2316 100-101 -1.6673066777490426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 16.0 5 50.0 6 114.0 7 225.0 8 619.0 9 1278.0 10 2090.0 11 3296.0 12 4994.0 13 7137.0 14 9207.0 15 12227.0 16 16472.0 17 22189.0 18 19635.0 19 13450.0 20 12496.0 21 13354.0 22 14246.0 23 15986.0 24 18664.0 25 22332.0 26 27625.0 27 34323.0 28 42641.0 29 53491.0 30 66547.0 31 83106.0 32 105379.0 33 136118.0 34 180944.0 35 253625.0 36 382415.0 37 558346.0 38 382731.0 39 26032.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.02118497089367 19.92570237058676 15.314899718650624 22.738212939868944 2 15.38810580585902 21.06613495311463 39.89290758501807 23.652851656008277 3 18.985910264839205 25.05705572035698 30.169224671349614 25.787809343454203 4 13.1398263351688 17.62456234850525 37.22026181437876 32.01534950194719 5 13.79937419444639 37.50834551575484 34.29462403304077 14.397656256758001 6 31.475155755707114 37.0029008345502 17.44849229733672 14.073451112405962 7 28.039447889955436 32.95317381495522 21.12026157050821 17.88711672458113 8 26.00513878049308 35.55359842415974 19.805772183352634 18.635490611994552 9 26.133963040855107 16.889969855347346 20.445442582241196 36.530624521556355 10-11 24.53015759182765 27.191431489899763 27.60304489334381 20.675366024928778 12-13 25.60350821803653 24.381934493578377 28.18149488509375 21.833062403291343 14-15 22.500910196799097 25.707967976799612 26.430090201878897 25.36103162452239 16-17 21.920190421969927 29.063353678770653 26.937855724061137 22.078600175198275 18-19 21.87543005252244 27.49782368849217 29.403207022074522 21.22353923691087 20-21 22.542193518404023 26.666327222085812 29.590045964596978 21.201433294913187 22-23 22.28077231869391 26.615098022101076 29.4708945406278 21.633235118577215 24-25 22.182183925785 26.85469178430278 29.45983294272566 21.503291347186554 26-27 22.098003862401313 27.111438515450686 29.19584946189629 21.59470816025171 28-29 21.85146650812525 26.9562092243424 29.51115609409307 21.681168173439282 30-31 22.063089426801394 26.781886248358205 29.479850174951245 21.675174149889155 32-33 21.86554039681825 27.015024489056106 29.358165371212568 21.761269742913075 34-35 22.259407530113318 26.96083799252812 29.20267757808505 21.577076899273514 36-37 22.20944421090548 26.831667516713548 29.138276608269692 21.820611664111283 38-39 22.02762597780894 27.03245063475499 29.26178030234517 21.678143085090905 40-41 22.273816297392635 26.8348653614838 29.23620496370043 21.65511337742313 42-43 22.11532506872592 26.956562939336195 29.002897665176647 21.925214326761242 44-45 22.218244513120542 27.086154426856023 28.811503770632317 21.884097289391118 46-47 22.377266864397733 27.248276733847653 28.403857348182232 21.97059905357238 48-49 22.479058709923347 27.4508863371905 28.13348717179495 21.9365677810912 50-51 22.392271203047827 27.439002453289884 28.062262723971564 22.10646361969072 52-53 22.63279781704373 27.487359141915746 27.923006754897106 21.956836286143417 54-55 22.452671470144576 27.425609885798952 28.000753332543045 22.120965311513427 56-57 22.42978858924506 27.726380932534923 27.92289031568104 21.920940162538972 58-59 22.57931216428824 27.47255771691528 27.887654378507996 22.060475740288478 60-61 22.549550199525793 27.40982513405747 28.159705560124017 21.880919106292723 62-63 22.455452917536316 27.493503294501526 28.139291253121556 21.911752534840602 64-65 22.36234302516237 27.41947089580937 28.07829534114331 22.13989073788495 66-67 22.300418260010048 27.692465264917665 27.90063199016747 22.10648448490482 68-69 22.19347875661521 27.57497896499933 28.033384708537458 22.198157569848 70-71 22.289765237725923 27.46137672547765 27.997389236695774 22.251468800100657 72-73 22.336403231365377 27.465622653610083 27.948707412562168 22.24926670246237 74-75 22.268372127038997 27.66295676216794 27.655309163513913 22.413361947279146 76-77 22.357207718970315 27.663542729705306 27.695823389795653 22.283426161528727 78-79 22.376678255292788 27.579095174909746 27.68209717498485 22.362129394812623 80-81 22.374749867352783 27.83246287794628 27.51129835824126 22.28148889645968 82-83 22.32627779903189 27.669633023879612 27.67649419120599 22.32759498588251 84-85 22.353372513286878 27.6305367732271 27.492610755123327 22.5234799583627 86-87 22.324394925544723 27.56317709402274 27.680893258881987 22.43153472155055 88-89 22.430154759054215 27.539098669365448 27.4971661647741 22.533580406806237 90-91 22.322427484100455 27.66348698715356 27.59141783379312 22.422667694952857 92-93 22.303002042146698 27.614352744866917 27.52862111455444 22.554024098431942 94-95 22.275945422383618 27.485730641856705 27.469865970062756 22.76845796569692 96-97 22.326160185342637 27.54144800409688 27.369650241458523 22.76274156910196 98-99 22.186157249528044 27.5685120351529 27.334730804608864 22.910599910710197 100-101 22.01109345097149 27.795867134152513 27.26104995591518 22.931989458960818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 636.0 1 471.0 2 347.5 3 451.5 4 419.0 5 370.0 6 659.5 7 1491.0 8 2551.5 9 3085.0 10 2799.5 11 2364.5 12 2438.5 13 2689.5 14 2903.0 15 3091.5 16 3276.0 17 3544.0 18 3805.5 19 4066.0 20 4502.5 21 5147.5 22 6173.0 23 7497.0 24 9299.5 25 11787.5 26 14714.0 27 17949.0 28 20998.5 29 24806.0 30 29269.0 31 33674.0 32 38335.5 33 43983.5 34 50742.5 35 55787.5 36 61444.0 37 67837.0 38 72372.0 39 75384.0 40 78011.5 41 82457.0 42 85558.0 43 88771.5 44 93775.5 45 97226.0 46 98189.0 47 100181.0 48 103922.5 49 103527.0 50 100288.0 51 96422.0 52 91117.0 53 86351.5 54 81245.0 55 74877.0 56 68563.0 57 60419.5 58 52948.0 59 47227.0 60 40194.0 61 31893.5 62 24060.0 63 18216.0 64 13810.0 65 10266.5 66 7162.0 67 5275.5 68 3609.0 69 1977.0 70 1207.0 71 731.5 72 421.0 73 259.0 74 171.0 75 106.5 76 60.0 77 22.0 78 11.5 79 10.5 80 6.5 81 5.5 82 4.0 83 2.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025949455179393302 2 0.007155758852499365 3 0.010930225060411119 4 3.931735633241409E-5 5 0.004875352185219348 6 0.0 7 0.0 8 3.931735633241409E-5 9 1.1795206899724227E-4 10-11 0.0 12-13 2.5556281616069163E-4 14-15 0.0 16-17 7.863471266482819E-5 18-19 0.004403543909230379 20-21 0.0013957661498007002 22-23 0.0 24-25 0.005759992702698665 26-27 0.006664291898344189 28-29 0.008728453105795928 30-31 0.03304623799739405 32-33 9.829339083103524E-4 34-35 0.005406136495706938 36-37 0.016080798739957367 38-39 0.0086498183931311 40-41 0.010340464715424907 42-43 0.005956579484360735 44-45 0.00259494551793933 46-47 0.00206416120745174 48-49 0.0017889397131248413 50-51 0.003400951322753819 52-53 0.00259494551793933 54-55 0.0015923529314627709 56-57 6.290777013186255E-4 58-59 8.846405174793171E-4 60-61 1.9658678166207046E-5 62-63 5.8976034498621136E-5 64-65 0.006448046438515912 66-67 4.718082759889691E-4 68-69 0.0010222512646427665 70-71 0.0034795860354186474 72-73 0.010635344887918013 74-75 0.004875352185219348 76-77 0.0034402686790862332 78-79 0.009888315117602145 80-81 2.162454598282775E-4 82-83 0.004206957127568308 84-85 5.307843104875902E-4 86-87 0.0 88-89 0.001297472758969665 90-91 6.094190231524185E-4 92-93 1.5726942532965637E-4 94-95 7.470297703158678E-4 96-97 3.341975288255198E-4 98-99 9.829339083103524E-5 100-101 5.307843104875902E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2543406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51200465966821 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92611546694584 39.55934635338376 2 14.548883499702232 15.570798432580716 3 4.906024162719962 7.875935635677463 4 2.1925282416261878 4.693063259294187 5 1.1979499903727553 3.2052352733438183 6 0.7208658623424987 2.314498643080052 7 0.46609281883396114 1.745909276529657 8 0.3403793647609131 1.4571505722512708 9 0.2530512610979898 1.2187152236709493 >10 1.3026771878872778 12.666678355660046 >50 0.09677252270871799 3.5297106628248995 >100 0.04615805091054142 4.357132541926525 >500 0.0020601165456970195 0.6930613313890925 >1k 2.2072677275168014E-4 0.17996837730846185 >5k 1.4715118183445343E-4 0.534432455389425 >10k+ 7.357559091722672E-5 0.3983636056895684 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10118 0.3978130113713658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8013 0.3150499762916341 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5561 0.21864381856455478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9658678166207046E-5 0.0 18-19 0.0 0.0 0.0 3.931735633241409E-5 0.0 20-21 0.0 0.0 0.0 7.863471266482819E-5 0.0 22-23 0.0 0.0 0.0 1.1795206899724228E-4 0.0 24-25 0.0 0.0 0.0 1.5726942532965637E-4 0.0 26-27 0.0 0.0 0.0 1.9658678166207047E-4 0.0 28-29 0.0 0.0 0.0 7.273710921496608E-4 0.0 30-31 0.0 0.0 0.0 0.0019068917821220836 0.0 32-33 0.0 0.0 0.0 0.003754807529745546 0.0 34-35 0.0 0.0 0.0 0.005897603449862114 0.0 36-37 0.0 0.0 0.0 0.007843812588316613 0.0 38-39 0.0 0.0 0.0 0.009770363048604902 0.0 40-41 0.0 0.0 0.0 0.011539644083563536 0.0 42-43 0.0 0.0 0.0 0.01431151770499873 0.0 44-45 0.0 0.0 0.0 0.01655260701594633 0.0 46-47 0.0 0.0 0.0 0.0212117137413374 0.0 48-49 0.0 0.0 0.0 0.027089658513033313 0.0 50-51 0.0 0.0 0.0 0.032102621445416105 0.0 52-53 0.0 0.0 0.0 0.0435046547818162 0.0 54-55 0.0 0.0 0.0 0.06308469823535841 0.0 56-57 0.0 0.0 0.0 0.09565912795676348 0.0 58-59 0.0 0.0 0.0 0.13660815457697278 0.0 60-61 0.0 0.0 0.0 0.1766135646452041 0.0 62-63 0.0 0.0 0.0 0.22353882942794034 0.0 64-65 0.0 0.0 0.0 0.28080455892610146 0.0 66-67 0.0 0.0 0.0 0.3602845947520765 0.0 68-69 0.0 0.0 0.0 0.4603865839744028 0.0 70-71 0.0 0.0 0.0 0.5547875565285291 0.0 72-73 0.0 0.0 0.0 0.6516851812097636 0.0 74-75 0.0 0.0 0.0 0.7547163134788548 0.0 76-77 0.0 0.0 0.0 0.8708794427629722 0.0 78-79 0.0 0.0 0.0 0.9750311196875372 0.0 80-81 0.0 0.0 0.0 1.0920592308109676 0.0 82-83 0.0 0.0 0.0 1.2235757877428928 0.0 84-85 0.0 0.0 0.0 1.3995406160086121 0.0 86-87 0.0 0.0 0.0 1.6065858144551046 0.0 88-89 0.0 0.0 0.0 1.8508448906702273 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5530 0.0 50.43245 1 GTATCAA 9640 0.0 40.069195 1 ATCAACG 12270 0.0 31.159801 3 TCAACGC 12345 0.0 30.968668 4 CAACGCA 12540 0.0 30.487099 5 TATCAAC 12650 0.0 30.338797 2 AACGCAG 12985 0.0 29.441137 6 ACGCAGA 14910 0.0 25.417597 7 CGCAGAG 14920 0.0 25.114086 8 GCAGAGT 15970 0.0 22.719442 9 GTACATG 9600 0.0 22.17195 1 GAGTACT 9410 0.0 21.752523 12-13 ACATGGG 9520 0.0 20.803791 3 TACATGG 9990 0.0 20.63487 2 AGAGTAC 14195 0.0 20.625616 10-11 AGTACTT 9865 0.0 20.388174 12-13 GTACTTT 9945 0.0 20.368233 14-15 CAGAGTA 15240 0.0 20.161764 10-11 GCACCGT 535 0.0 18.64087 6 TACTTTT 11485 0.0 17.20289 14-15 >>END_MODULE