FastQCFastQC Report
Thu 26 May 2016
SRR936789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574455
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97610.3791482080673385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70770.27489313272129445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.18640838546410793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.047.5159571
GTATCAA90650.044.5019841
ATCAACG112250.035.5152663
TCAACGC113650.035.119584
TATCAAC114250.035.0599142
CAACGCA116500.034.2196465
AACGCAG120750.033.2113346
ACGCAGA136550.028.7763967
CGCAGAG137150.028.37288
GCAGAGT146150.025.5502829
GTACATG99000.023.3740121
TACATGG100750.022.119092
ACATGGG99400.021.702463
GAGTACT79900.021.64156212-13
ATCGCGA550.002882515521.58599754-55
GTGGTAT25400.021.5131281
AGAGTAC131350.020.8874510-11
AGTACTT83750.020.5048912-13
CAGAGTA139300.020.4456810-11
GTACTTT84350.020.41436214-15
Produced by FastQC (version 0.11.5)