FastQCFastQC Report
Thu 26 May 2016
SRR936789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574455
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99610.3869168425938694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76940.29885937023564213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55820.21682258963547624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56900.043.9214481
GTATCAA95800.039.52721
ATCAACG124550.029.8585973
TATCAAC126150.029.6327922
TCAACGC125950.029.5261274
CAACGCA128850.028.8677585
AACGCAG131750.028.2683946
ACGCAGA151250.024.4668337
CGCAGAG151700.024.1437388
GTACATG93350.022.2927171
CGCGGAA3450.022.02769982-83
GCAGAGT162550.021.7138869
TACATGG96050.020.9183832
AGAGTAC141050.020.84746610-11
ACATGGG94600.020.434063
AGTACTT96000.019.9872512-13
GAGTACT92800.019.78082712-13
GTACTTT95900.019.04309514-15
TCGCGCT4050.018.76451760-61
TGGTATC30000.018.6829662
Produced by FastQC (version 0.11.5)