##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574455 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.091034413108794 31.0 31.0 34.0 30.0 34.0 2 32.222718594809386 33.0 31.0 34.0 30.0 34.0 3 32.33360730717764 34.0 31.0 34.0 30.0 34.0 4 36.000942723799795 37.0 35.0 37.0 35.0 37.0 5 35.895669957330774 37.0 35.0 37.0 35.0 37.0 6 35.93410799567287 37.0 35.0 37.0 35.0 37.0 7 35.874838363847886 37.0 35.0 37.0 35.0 37.0 8 35.83252999178467 37.0 35.0 37.0 35.0 37.0 9 37.502278734722495 39.0 37.0 39.0 35.0 39.0 10-11 37.40508690188797 39.0 37.0 39.0 34.0 39.0 12-13 37.302977523398155 39.0 37.0 39.0 34.0 39.0 14-15 38.485512079255614 40.0 38.0 41.0 34.0 41.0 16-17 38.38469850900482 40.0 38.0 41.0 33.5 41.0 18-19 38.54020812171896 40.0 38.0 41.0 34.0 41.0 20-21 38.5670741962862 40.0 38.0 41.0 34.0 41.0 22-23 38.49126552998596 40.0 38.0 41.0 34.0 41.0 24-25 38.3830199012995 40.0 38.0 41.0 34.0 41.0 26-27 38.20226611069139 40.0 38.0 41.0 33.5 41.0 28-29 38.120956862714635 40.0 37.5 41.0 33.5 41.0 30-31 38.12801078286472 40.0 37.5 41.0 33.5 41.0 32-33 37.98252989467674 40.0 37.0 41.0 33.0 41.0 34-35 37.84549467751427 40.0 37.0 41.0 33.0 41.0 36-37 37.693408896251825 40.0 37.0 41.0 33.0 41.0 38-39 37.553646305722964 40.0 37.0 41.0 33.0 41.0 40-41 37.379338539613244 40.0 36.5 41.0 32.0 41.0 42-43 37.134466906587996 39.5 36.0 41.0 31.0 41.0 44-45 36.89271438032516 39.0 36.0 41.0 31.0 41.0 46-47 36.57379076348198 39.0 36.0 41.0 30.5 41.0 48-49 36.246650261900086 39.0 35.0 40.0 30.0 41.0 50-51 35.97085946345926 39.0 35.0 40.0 29.0 41.0 52-53 35.72569223389028 38.5 35.0 40.0 28.5 41.0 54-55 35.430794867263174 38.0 35.0 40.0 28.0 41.0 56-57 35.13750366582442 38.0 34.0 40.0 27.0 41.0 58-59 34.77093734402038 38.0 34.0 40.0 26.0 41.0 60-61 34.43202736113081 37.5 34.0 40.0 26.0 41.0 62-63 34.04116677121954 37.0 33.0 39.5 26.0 41.0 64-65 33.64270865095719 36.0 33.0 39.0 25.0 40.0 66-67 33.2302331949869 36.0 32.0 39.0 24.0 40.0 68-69 32.90554428024572 35.5 32.0 38.5 23.0 40.0 70-71 32.43106715790332 35.0 31.0 38.0 22.0 40.0 72-73 31.902032857439732 35.0 31.0 37.0 20.5 39.0 74-75 31.35645020013945 34.5 30.5 37.0 19.5 39.0 76-77 29.97965647098124 33.0 28.5 35.0 18.5 37.5 78-79 30.740717549928043 34.0 30.0 36.0 19.0 38.0 80-81 30.508903437814993 34.0 30.0 35.5 18.0 37.0 82-83 30.149114861203635 34.0 30.0 35.0 15.0 37.0 84-85 29.7577759176214 34.0 29.5 35.0 11.0 36.0 86-87 29.366966989129736 34.0 29.0 35.0 7.0 36.0 88-89 28.9410046009738 34.0 29.0 35.0 4.0 35.5 90-91 28.42634984880295 33.0 28.5 35.0 2.0 35.0 92-93 27.875268163553063 33.0 27.0 35.0 2.0 35.0 94-95 27.216182648366352 32.5 26.0 34.0 2.0 35.0 96-97 26.469606382710126 32.0 25.0 34.0 2.0 35.0 98-99 25.5108156872037 32.0 23.0 34.0 2.0 35.0 100-101 21.805169832061544 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14149947395824114 1101 2 0.07846406605327161 1101 3 0.142072623597727 1101 4 0.15122574106787567 1101 5 0.0995298157299942 1101 6 0.1417966301667377 1101 7 0.1907023234995151 1101 8 0.1841006539269756 1101 9 0.2224118098265251 1101 10-11 0.24559125334746312 1101 12-13 0.28456323711026243 1101 14-15 0.2874214408130271 1101 16-17 0.3359358756865163 1101 18-19 0.31482346181761756 1101 20-21 0.2093668424600068 1101 22-23 0.26630427425241265 1101 24-25 0.3324127504169354 1101 26-27 0.32671937392108674 1101 28-29 0.3179320270310413 1101 30-31 0.34875448647575524 1101 32-33 0.34459025374292906 1101 34-35 0.3439175153865648 1101 36-37 0.36420320193281697 1101 38-39 0.2677983879597434 1101 40-41 0.3537185961417393 1101 42-43 0.26291075322856017 1101 44-45 0.21295558022331562 1101 46-47 0.23931173993397437 1101 48-49 0.26411167262553903 1101 50-51 0.2972081302391629 1101 52-53 0.31781011403069925 1101 54-55 0.3202195659586309 1101 56-57 0.3357244140911888 1101 58-59 0.2740009991914718 1101 60-61 0.44583438052668356 1101 62-63 0.36046396962974114 1101 64-65 0.4954474887377387 1101 66-67 0.17275998974254492 1101 68-69 0.08739484147556453 1101 70-71 0.05454493322434928 1101 72-73 0.36848811290086303 1101 74-75 0.3075757674751216 1101 76-77 0.08884684111428953 1101 78-79 0.2313146126017145 1101 80-81 0.43034276016762973 1101 82-83 0.3723467887625418 1101 84-85 0.2831465066329706 1101 86-87 0.07351701663072063 1101 88-89 0.20433454682565255 1101 90-91 0.4418518421774209 1101 92-93 0.6228947392262825 1101 94-95 0.40133016624638174 1101 96-97 0.40306067219734487 1101 98-99 0.7429832863848311 1101 100-101 1.1806654427661094 1102 1 0.23110615197307283 1102 2 0.24459082441036628 1102 3 0.2656667211571744 1102 4 0.16856755419902925 1102 5 0.20726571667370308 1102 6 0.1561837419879808 1102 7 0.15106866747436953 1102 8 0.24674254955863262 1102 9 0.2302963509044602 1102 10-11 0.2889530963458853 1102 12-13 0.3333749862948352 1102 14-15 0.491737226256042 1102 16-17 0.4733601196930479 1102 18-19 0.3884984934079938 1102 20-21 0.42407803850833403 1102 22-23 0.42798575271699946 1102 24-25 0.36712700977482626 1102 26-27 0.3124652375829271 1102 28-29 0.3512329867520023 1102 30-31 0.37300092972968457 1102 32-33 0.39470843483325524 1102 34-35 0.3322073183948788 1102 36-37 0.314829613927067 1102 38-39 0.4324495361786731 1102 40-41 0.359985152807738 1102 42-43 0.4385692777576651 1102 44-45 0.2718738142253585 1102 46-47 0.32351063226908394 1102 48-49 0.29018148127312315 1102 50-51 0.3309031429623417 1102 52-53 0.4320280476195464 1102 54-55 0.40789078929826417 1102 56-57 0.33704511823466277 1102 58-59 0.370115817603363 1102 60-61 0.49713367631358807 1102 62-63 0.5307376833007993 1102 64-65 0.4076812331811439 1102 66-67 0.3696434203707639 1102 68-69 0.2880346554491311 1102 70-71 0.14045719029171266 1102 72-73 0.30093064973890193 1102 74-75 0.47290954638075533 1102 76-77 0.3744252670605235 1102 78-79 0.4019945628925541 1102 80-81 0.5256106431074308 1102 82-83 0.47310739460162665 1102 84-85 0.5148507162468867 1102 86-87 0.25222597089342 1102 88-89 0.41347504482231656 1102 90-91 0.549512900898975 1102 92-93 0.40067112459062315 1102 94-95 0.5008779799832368 1102 96-97 0.7448922528302653 1102 98-99 0.9373332207673464 1102 100-101 1.248328729349165 1103 1 0.24176403200719676 1103 2 0.24105504967956648 1103 3 0.2765487638857209 1103 4 0.18295144064870072 1103 5 0.17808777373775797 1103 6 0.1961477941954044 1103 7 0.08134207782700287 1103 8 0.1571937093450657 1103 9 0.27219419812497847 1103 10-11 0.22608752037721302 1103 12-13 0.2707273727758519 1103 14-15 0.39860055650969883 1103 16-17 0.29988796901210435 1103 18-19 0.31041445079392105 1103 20-21 0.31284053779292975 1103 22-23 0.27517840454123643 1103 24-25 0.23255880572000365 1103 26-27 0.2853433911909278 1103 28-29 0.2916047561519548 1103 30-31 0.2612032266818929 1103 32-33 0.2997764876293658 1103 34-35 0.3337825212314556 1103 36-37 0.31526778726682636 1103 38-39 0.27692082563880405 1103 40-41 0.47815691185123654 1103 42-43 0.6085400616593759 1103 44-45 0.45512817960645435 1103 46-47 0.5497036683722243 1103 48-49 0.6190378290973158 1103 50-51 0.6074385552172927 1103 52-53 0.62320687085397 1103 54-55 0.4854854213852846 1103 56-57 0.598974184709455 1103 58-59 0.6429524772657942 1103 60-61 0.3945335253274109 1103 62-63 0.5173306420030883 1103 64-65 0.3337372506889906 1103 66-67 0.24692565551129775 1103 68-69 0.24648150095538313 1103 70-71 0.2798833883016414 1103 72-73 0.30107884240162264 1103 74-75 0.6253998644601211 1103 76-77 0.37197685173406114 1103 78-79 0.5138894258194533 1103 80-81 0.6250118084809024 1103 82-83 0.6393754059719221 1103 84-85 0.6144431353234729 1103 86-87 0.6049687956431633 1103 88-89 0.39003777903485926 1103 90-91 0.44880505098033296 1103 92-93 0.5539889368717397 1103 94-95 0.14141940443154866 1103 96-97 0.2907400647570846 1103 98-99 0.1435299906442431 1103 100-101 0.05152205979359081 1104 1 0.19624201422757892 1104 2 0.20534748300600825 1104 3 0.17172604319107165 1104 4 0.06894689237497431 1104 5 0.09732490419051487 1104 6 -0.007159007541211793 1104 7 0.1148923155776771 1104 8 0.13567313874345643 1104 9 0.19659982144521848 1104 10-11 0.22575596155976285 1104 12-13 0.05694506969543056 1104 14-15 -0.007639623353860259 1104 16-17 0.16049423322546374 1104 18-19 0.10607214151130506 1104 20-21 0.14641661800794026 1104 22-23 0.1460177677829151 1104 24-25 0.13390700708448833 1104 26-27 0.05379258490604144 1104 28-29 0.05429392299090807 1104 30-31 0.06948263540630961 1104 32-33 0.11224340927501686 1104 34-35 0.12840754707391966 1104 36-37 0.18201215360959822 1104 38-39 0.1360724175345993 1104 40-41 0.1384644371768573 1104 42-43 0.03937167216175652 1104 44-45 -0.07185264528499857 1104 46-47 0.05620769979665141 1104 48-49 -0.1660880237040061 1104 50-51 -0.02485418821871832 1104 52-53 -0.05573358810819684 1104 54-55 -0.2278009648045085 1104 56-57 -0.19154783307015322 1104 58-59 -0.1780887156223585 1104 60-61 0.014341774242019767 1104 62-63 -0.014641654875141796 1104 64-65 -0.14416434120681032 1104 66-67 -0.1344287480446269 1104 68-69 -0.19354356623502156 1104 70-71 -0.37096734562435074 1104 72-73 -0.1359963074846675 1104 74-75 -0.16039789241397528 1104 76-77 -0.14929614805919655 1104 78-79 -0.24839789069904938 1104 80-81 0.04983246177840073 1104 82-83 0.08106576156423273 1104 84-85 0.16965297613389296 1104 86-87 0.016928688138509784 1104 88-89 -0.17656161108298107 1104 90-91 -0.041200375937179956 1104 92-93 0.025825819236843017 1104 94-95 0.13409125681939216 1104 96-97 -0.011589116553647472 1104 98-99 -0.06931965789824446 1104 100-101 0.2163163537774686 1105 1 0.21186070780166233 1105 2 0.20717037201291078 1105 3 0.11677251159255775 1105 4 0.14011524075522175 1105 5 0.1310211336116538 1105 6 0.154420918109345 1105 7 0.12298180044225404 1105 8 0.1161059587115929 1105 9 0.19482074550257522 1105 10-11 0.21590266894160237 1105 12-13 0.15039169741714886 1105 14-15 0.23416401445814472 1105 16-17 0.2234668832520157 1105 18-19 0.14888746280710308 1105 20-21 0.17760753675461416 1105 22-23 0.20187278637028072 1105 24-25 0.15956884988851527 1105 26-27 0.23391141124698578 1105 28-29 0.14652838926811995 1105 30-31 0.16301556158793318 1105 32-33 0.1688166965825033 1105 34-35 0.24621224702082145 1105 36-37 0.23095931239876677 1105 38-39 0.25902316102902745 1105 40-41 0.13501648178651493 1105 42-43 0.18875165091905188 1105 44-45 0.3773341576889777 1105 46-47 0.43065538965327477 1105 48-49 0.3974256512827168 1105 50-51 0.3373338096570109 1105 52-53 0.3307188813554305 1105 54-55 0.3634277924976672 1105 56-57 0.4160039460057945 1105 58-59 0.37179113036808076 1105 60-61 0.29756668130522 1105 62-63 0.48093720864052614 1105 64-65 0.22356800080062555 1105 66-67 0.2967256598151735 1105 68-69 0.33769581241606517 1105 70-71 0.05130296770544618 1105 72-73 0.23564070791628922 1105 74-75 0.410285193087887 1105 76-77 0.18340565757011973 1105 78-79 0.38293044174917057 1105 80-81 0.3892018191867912 1105 82-83 0.48772070049147587 1105 84-85 0.5605451694424453 1105 86-87 0.1818782367311833 1105 88-89 0.13707819242206298 1105 90-91 0.1176518545460894 1105 92-93 0.14319172470683839 1105 94-95 0.0299509222469041 1105 96-97 0.03812344319144145 1105 98-99 0.022592848740099924 1105 100-101 0.4099754084774112 1106 1 0.20161365365826356 1106 2 0.161339839358142 1106 3 0.16335888451948222 1106 4 0.14653782542716698 1106 5 0.1995652837371651 1106 6 0.20883730242189102 1106 7 0.1429985359255923 1106 8 0.17338795160586784 1106 9 0.25946223578364425 1106 10-11 0.2755866152234532 1106 12-13 0.2788955177713319 1106 14-15 0.3904672559292166 1106 16-17 0.29571958905044937 1106 18-19 0.13467190429464182 1106 20-21 0.32528720314235926 1106 22-23 0.22618381944342758 1106 24-25 0.2311150988082602 1106 26-27 0.16099408727820474 1106 28-29 0.22249239426133016 1106 30-31 0.2521539585481065 1106 32-33 0.17796428434090927 1106 34-35 0.02827510110660114 1106 36-37 0.12889275455847127 1106 38-39 0.23570802639068944 1106 40-41 0.2782338098705779 1106 42-43 0.19009254722764268 1106 44-45 -0.0392188814473613 1106 46-47 -0.048845015016205195 1106 48-49 0.014712582516338557 1106 50-51 0.12851955980447372 1106 52-53 0.13346514673629173 1106 54-55 -0.08737331588515929 1106 56-57 0.02090867720923484 1106 58-59 -0.038920950730421566 1106 60-61 0.03634361926047802 1106 62-63 0.15664195081805588 1106 64-65 -0.07615696113300885 1106 66-67 0.07245646545037232 1106 68-69 0.1668155534275968 1106 70-71 0.11367419364819398 1106 72-73 0.15641671307410832 1106 74-75 0.19905321459709668 1106 76-77 0.15706784772361715 1106 78-79 0.3078402484680538 1106 80-81 0.3831310911932668 1106 82-83 0.48509515007240367 1106 84-85 0.39566773628149576 1106 86-87 0.31134091797509456 1106 88-89 0.38033941685586825 1106 90-91 0.3881123546901257 1106 92-93 0.5397952446453829 1106 94-95 0.5622785261920846 1106 96-97 0.605973482286629 1106 98-99 0.5248836894923734 1106 100-101 0.8326526742835263 1107 1 0.08483941682498397 1107 2 0.08586047001899288 1107 3 0.07886240682743306 1107 4 0.03619364562172933 1107 5 5.716574372698346E-4 1107 6 0.014931901549701365 1107 7 -0.028750541565180754 1107 8 0.021452359522676545 1107 9 0.07937904222443848 1107 10-11 0.09162284467665671 1107 12-13 0.025568446318800397 1107 14-15 0.09952596106172251 1107 16-17 0.04416306439429718 1107 18-19 0.043770193459359064 1107 20-21 0.009475059566376842 1107 22-23 0.0573814041465468 1107 24-25 -0.0014598760323991655 1107 26-27 0.08343219529565005 1107 28-29 0.13383288402986437 1107 30-31 0.07145016787384151 1107 32-33 0.022223928755529698 1107 34-35 -0.048998297081922715 1107 36-37 -0.05405927496182272 1107 38-39 0.038276962989151286 1107 40-41 0.007146255358669862 1107 42-43 0.10726128255137013 1107 44-45 -0.08596628164863773 1107 46-47 -0.12092217033320907 1107 48-49 0.09616846980248539 1107 50-51 -0.09545159081611843 1107 52-53 -0.11570761408221841 1107 54-55 0.03415033389679678 1107 56-57 0.02577684225452259 1107 58-59 0.016304141520492976 1107 60-61 0.0012963196965714019 1107 62-63 -0.021070226303720574 1107 64-65 -0.07361888666135741 1107 66-67 -0.03465926752514292 1107 68-69 0.038329810388347596 1107 70-71 0.008967719310717825 1107 72-73 -0.13039241138077173 1107 74-75 -0.07964789241397341 1107 76-77 0.06281424155119453 1107 78-79 0.128572888521731 1107 80-81 0.20652726697320745 1107 82-83 0.139231345979816 1107 84-85 0.025471157952079437 1107 86-87 -0.15992845471863149 1107 88-89 -0.0795713513427252 1107 90-91 -0.005495830482633579 1107 92-93 0.041877767288792 1107 94-95 0.09188671136484672 1107 96-97 0.12762192240739623 1107 98-99 -0.22281641114499706 1107 100-101 -0.0484368929757828 1108 1 0.15404233595452865 1108 2 0.09499008069165171 1108 3 0.0947727195441388 1108 4 0.11171945217986234 1108 5 0.11584755480542697 1108 6 0.06912323001781573 1108 7 0.1316170574177491 1108 8 0.1440346664275438 1108 9 0.12524649260914344 1108 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-1.1576305201070554 2316 80-81 -1.1495164314999364 2316 82-83 -1.1678583800729037 2316 84-85 -1.3716646326501625 2316 86-87 -1.2523212973375841 2316 88-89 -1.4701596910225625 2316 90-91 -1.5293180050547086 2316 92-93 -1.5539162361863816 2316 94-95 -1.4472939607487234 2316 96-97 -1.1766346490000537 2316 98-99 -1.487128363527713 2316 100-101 -1.553357148807791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 28.0 5 53.0 6 110.0 7 264.0 8 661.0 9 1331.0 10 2235.0 11 3439.0 12 5186.0 13 7357.0 14 9467.0 15 12989.0 16 17097.0 17 22288.0 18 20691.0 19 14223.0 20 13511.0 21 14421.0 22 15457.0 23 17357.0 24 20266.0 25 24669.0 26 30089.0 27 37095.0 28 46375.0 29 57640.0 30 70820.0 31 88576.0 32 111605.0 33 143597.0 34 191593.0 35 266297.0 36 397941.0 37 554156.0 38 337639.0 39 17918.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.01607830353733 19.951907292739588 15.298205479180613 22.733808924542473 2 15.403249082187306 21.08916233252419 39.848162257553135 23.659426327735368 3 19.006474783536206 25.03592030760757 30.076921314036518 25.880683594819708 4 13.16901312710118 17.582003111350215 37.170263882118284 32.078719879430324 5 13.811039618093925 37.490614518412634 34.28725691457027 14.41108894892317 6 31.496685706295118 37.000374836615904 17.459306921270716 14.04363253581826 7 28.03529290665403 32.96868657638218 21.089473306000688 17.906547210963097 8 26.039608383133515 35.54464925586192 19.78795512059834 18.62778724040622 9 26.076985644334684 16.949380334626294 20.4385473580029 36.535086663036125 10-11 24.556769066175217 27.16485650109589 27.58380772646364 20.694566706265253 12-13 25.574830400997495 24.38772478058463 28.156289389404748 21.88115542901313 14-15 22.512143346844283 25.68184334160046 26.43883851145194 25.36717480010332 16-17 21.958511607311063 29.094623910691773 26.900761520399463 22.046102961597697 18-19 21.919218815214208 27.505036515587705 29.339409974602326 21.236334694595755 20-21 22.553946459941095 26.65377059559844 29.527924169548374 21.264358774912093 22-23 22.331289749568732 26.637692913957782 29.338226125787024 21.692791210686455 24-25 22.19607593296769 26.87166048058196 29.370503321139864 21.561760265310486 26-27 22.122815423762294 27.109576038626027 29.12431659309726 21.64329194451442 28-29 21.853096635297476 27.00777545473349 29.44645404499412 21.692673864974914 30-31 22.06563523966509 26.741569716684836 29.48336006777516 21.70943497587491 32-33 21.921331665850598 26.970044160842033 29.309828792977772 21.798795380329594 34-35 22.269470625821775 26.946712993623894 29.11923106055456 21.664585319999766 36-37 22.280170240404747 26.844135089086418 29.033905903174606 21.84178876733423 38-39 22.058092562963978 27.07023151909727 29.176033025264065 21.69564289267469 40-41 22.27394475766488 26.881408845213905 29.16732134474535 21.677325052375863 42-43 22.147056367269965 26.99569423431367 28.88990096933137 21.96734842908499 44-45 22.25511807353401 27.06965163500624 28.74855066412114 21.9266796273386 46-47 22.447122645320473 27.24828778829481 28.3324425274855 21.972147038899212 48-49 22.493021604475498 27.4210934386379 28.099821548850322 21.986063408036284 50-51 22.381245758534305 27.44711448257323 28.02544937685658 22.14619038203588 52-53 22.62003025883148 27.47039664705734 27.926745660732077 21.982827433379104 54-55 22.54687574957208 27.387254162206865 27.918079867991796 22.147790220229254 56-57 22.479733963232327 27.783008808719455 27.80647075937866 21.930786468669556 58-59 22.619401370469074 27.483067237352333 27.791580094676643 22.10595129750195 60-61 22.561188777234396 27.367979459371274 28.095874394521463 21.974957368872868 62-63 22.444388334806213 27.457702504540027 28.13329707787478 21.96461208277898 64-65 22.387780552260896 27.43980421475859 28.010740920354554 22.161674312625962 66-67 22.32665656203897 27.665244457150763 27.88923857150293 22.11886040930734 68-69 22.233398311752303 27.54058205566288 27.991145274252364 22.234874358332448 70-71 22.272966954853157 27.431210567765905 27.968396918544187 22.32742555883675 72-73 22.424047031313425 27.470377225471022 27.88184295316873 22.223732790046828 74-75 22.276501240068285 27.657912062941474 27.637733034758817 22.427853662231424 76-77 22.388194782973482 27.62559454331111 27.651153350903396 22.335057322812013 78-79 22.399046149083293 27.55481700345303 27.63269903179626 22.413437815667418 80-81 22.289036116404688 27.81937757875822 27.58207938321891 22.30950692161818 82-83 22.368697326990123 27.69039880615128 27.5879978812798 22.352905985578793 84-85 22.359601454553367 27.65597583151048 27.435427099272374 22.54899561466378 86-87 22.377085477887622 27.536756851244004 27.633383184104726 22.452774486763648 88-89 22.432666594547605 27.564740615118627 27.414009186052223 22.588583604281542 90-91 22.349343817951723 27.644499498531328 27.5668320619879 22.439324621529046 92-93 22.350292751906768 27.589509912701725 27.472727664691032 22.587469670700475 94-95 22.314800608284084 27.51057990914582 27.383174302910714 22.791445179659384 96-97 22.356817135233705 27.508772461375823 27.32535047602511 22.80905992736536 98-99 22.19321453084645 27.52108927355479 27.29445508548939 22.99124111010937 100-101 22.05026316300569 27.78374021635689 27.199537765347937 22.966458855289478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 615.0 1 454.0 2 341.0 3 418.0 4 361.0 5 349.5 6 641.0 7 1431.0 8 2442.5 9 2885.0 10 2671.5 11 2388.5 12 2413.5 13 2610.0 14 2872.5 15 3112.0 16 3299.0 17 3535.5 18 3789.0 19 4110.0 20 4598.5 21 5279.0 22 6267.0 23 7619.0 24 9433.0 25 11790.5 26 14725.0 27 18131.5 28 21160.5 29 24787.0 30 29481.0 31 33831.0 32 38628.0 33 44396.0 34 50821.0 35 56142.5 36 61740.0 37 67798.0 38 72948.0 39 76082.5 40 78573.0 41 83296.5 42 86530.5 43 89988.5 44 94812.0 45 98201.5 46 99379.0 47 101559.0 48 105707.0 49 105507.0 50 102234.5 51 98218.5 52 92663.0 53 87773.0 54 82842.5 55 76150.0 56 69079.0 57 61076.0 58 53839.5 59 48099.5 60 41099.5 61 32528.5 62 24574.0 63 18693.0 64 14134.5 65 10556.5 66 7336.0 67 5336.5 68 3587.0 69 1915.0 70 1191.5 71 762.0 72 453.0 73 269.5 74 156.0 75 93.5 76 65.0 77 36.0 78 20.5 79 10.0 80 5.0 81 5.0 82 3.5 83 2.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026257984699674303 2 0.0049719260969797495 3 2.3305903579592575E-4 4 3.8843172632654286E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 3.884317263265429E-5 10-11 0.01122567689083709 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.524806221122529E-4 20-21 0.001068187247397993 22-23 0.0021752176674286403 24-25 0.010235175988704406 26-27 0.004991347683296076 28-29 2.913237947449072E-4 30-31 0.0023500119442755847 32-33 0.00448638643907157 34-35 0.0 36-37 0.008273595770755364 38-39 0.011827746066643231 40-41 0.001340089455826573 42-43 0.0 44-45 0.0 46-47 2.7190220842858003E-4 48-49 0.0031462969832449974 50-51 0.006525653002285921 52-53 0.0 54-55 0.002699600497969473 56-57 0.0028743947748164174 58-59 3.1074538106123435E-4 60-61 0.001747942768469443 62-63 0.005049612442245058 64-65 5.8264758948981436E-5 66-67 0.0022140608400612943 68-69 4.0785331264287005E-4 70-71 8.739713842347215E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001883893872683733 80-81 0.0022140608400612943 82-83 0.010079803298173788 84-85 0.007535575490734932 86-87 0.0040785331264287005 88-89 3.301669673775615E-4 90-91 6.60333934755123E-4 92-93 2.1363744947959862E-4 94-95 0.0 96-97 0.0024665414621735473 98-99 0.0016314132505714802 100-101 1.9421586316327143E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2574455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.462106055668514 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0757269051264 39.602443679526054 2 14.381940506624613 15.3777765730296 3 4.842949762931408 7.767428815443409 4 2.2317948736057818 4.772658169088383 5 1.202624130492984 3.214740940476102 6 0.7416036885228564 2.3788617028250343 7 0.476602610607515 1.7836125520295214 8 0.34762400980602054 1.4867769343796977 9 0.24688146976499167 1.1878922987779772 >10 1.3055002993395448 12.670603122385362 >50 0.09802270823507765 3.601246163224632 >100 0.046326944628206086 4.412121233824908 >500 0.0019653466210709765 0.6651808656745574 >1k 2.1837184678375157E-4 0.17437988995882955 >5k 2.1837184678375157E-4 0.9042770593557962 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9961 0.3869168425938694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7694 0.29885937023564213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5582 0.21682258963547624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.884317263265429E-5 0.0 10-11 0.0 0.0 0.0 3.884317263265429E-5 0.0 12-13 0.0 0.0 0.0 3.884317263265429E-5 0.0 14-15 0.0 0.0 0.0 3.884317263265429E-5 0.0 16-17 0.0 0.0 0.0 3.884317263265429E-5 0.0 18-19 0.0 0.0 0.0 7.768634526530859E-5 0.0 20-21 0.0 0.0 0.0 7.768634526530859E-5 0.0 22-23 3.884317263265429E-5 0.0 0.0 1.1652951789796287E-4 0.0 24-25 3.884317263265429E-5 0.0 0.0 1.5537269053061717E-4 0.0 26-27 3.884317263265429E-5 0.0 0.0 3.301669673775615E-4 0.0 28-29 3.884317263265429E-5 0.0 0.0 7.380202800204315E-4 0.0 30-31 3.884317263265429E-5 0.0 0.0 0.0016702564232041345 0.0 32-33 3.884317263265429E-5 0.0 0.0 0.003223983328510306 0.0 34-35 3.884317263265429E-5 0.0 0.0 0.004719445474867497 0.0 36-37 3.884317263265429E-5 0.0 0.0 0.006622760933867556 0.0 38-39 3.884317263265429E-5 0.0 0.0 0.008293017357071691 0.0 40-41 3.884317263265429E-5 0.0 0.0 0.010565342956081967 0.0 42-43 3.884317263265429E-5 0.0 0.0 0.013536845662480021 0.0 44-45 3.884317263265429E-5 0.0 0.0 0.01625586774676582 0.0 46-47 3.884317263265429E-5 0.0 0.0 0.02095589163531699 0.0 48-49 3.884317263265429E-5 0.0 0.0 0.02596666090492939 0.0 50-51 3.884317263265429E-5 0.0 0.0 0.030899743829276488 0.0 52-53 3.884317263265429E-5 0.0 0.0 0.04086301760955231 0.0 54-55 3.884317263265429E-5 0.0 0.0 0.05958542681849169 0.0 56-57 3.884317263265429E-5 0.0 0.0 0.09211658389833965 0.0 58-59 3.884317263265429E-5 0.0 0.0 0.1297944613520143 0.0 60-61 3.884317263265429E-5 0.0 0.0 0.16615167093617872 0.0 62-63 3.884317263265429E-5 0.0 0.0 0.21253041905956793 0.0 64-65 3.884317263265429E-5 0.0 0.0 0.26970756917483507 0.0 66-67 3.884317263265429E-5 0.0 0.0 0.34556828532640893 0.0 68-69 3.884317263265429E-5 0.0 0.0 0.4389666939216261 0.0 70-71 3.884317263265429E-5 0.0 0.0 0.526053086964037 0.0 72-73 3.884317263265429E-5 0.0 0.0 0.6173733858234073 0.0 74-75 3.884317263265429E-5 0.0 0.0 0.7165788487272063 0.0 76-77 3.884317263265429E-5 0.0 0.0 0.8307000899219447 0.0 78-79 3.884317263265429E-5 0.0 0.0 0.9368196375543563 0.0 80-81 3.884317263265429E-5 0.0 0.0 1.0480276408016453 0.0 82-83 3.884317263265429E-5 0.0 0.0 1.178016318016823 0.0 84-85 3.884317263265429E-5 0.0 0.0 1.346032461239369 0.0 86-87 3.884317263265429E-5 0.0 0.0 1.5433169350406204 0.0 88-89 3.884317263265429E-5 0.0 0.0 1.7812313674156277 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5690 0.0 43.921448 1 GTATCAA 9580 0.0 39.5272 1 ATCAACG 12455 0.0 29.858597 3 TATCAAC 12615 0.0 29.632792 2 TCAACGC 12595 0.0 29.526127 4 CAACGCA 12885 0.0 28.867758 5 AACGCAG 13175 0.0 28.268394 6 ACGCAGA 15125 0.0 24.466833 7 CGCAGAG 15170 0.0 24.143738 8 GTACATG 9335 0.0 22.292717 1 CGCGGAA 345 0.0 22.027699 82-83 GCAGAGT 16255 0.0 21.713886 9 TACATGG 9605 0.0 20.918383 2 AGAGTAC 14105 0.0 20.847466 10-11 ACATGGG 9460 0.0 20.43406 3 AGTACTT 9600 0.0 19.98725 12-13 GAGTACT 9280 0.0 19.780827 12-13 GTACTTT 9590 0.0 19.043095 14-15 TCGCGCT 405 0.0 18.764517 60-61 TGGTATC 3000 0.0 18.682966 2 >>END_MODULE