FastQCFastQC Report
Thu 26 May 2016
SRR936788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566095
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100300.390866277359178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70740.275671789236174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.18198858576942786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.046.4137571
GTATCAA88050.043.456971
ATCAACG110800.034.1085053
TATCAAC112800.033.714462
TCAACGC114050.033.0531734
CAACGCA116500.032.317265
AACGCAG119150.031.5929476
ACGCAGA136550.027.248617
CGCAGAG136050.027.1037258
GTACATG95200.025.0146081
GCAGAGT146400.024.0830639
TACATGG97550.023.6834132
ACATGGG93550.023.3748783
GAGTACT80500.021.36851312-13
AGAGTAC131150.020.8869810-11
AGTACTT85100.020.18553712-13
GTACTTT87350.019.4757614-15
GTGGTAT28200.019.3839231
CAGAGTA141350.019.22847710-11
CATGGGG49800.018.9004064
Produced by FastQC (version 0.11.5)