##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936788_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2566095 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543255803078218 31.0 31.0 34.0 30.0 34.0 2 31.640864815994732 33.0 31.0 34.0 30.0 34.0 3 31.661055416888306 33.0 31.0 34.0 30.0 34.0 4 35.26087966345751 37.0 35.0 37.0 33.0 37.0 5 35.25687903214807 37.0 35.0 37.0 33.0 37.0 6 35.30759695178861 37.0 35.0 37.0 33.0 37.0 7 35.24032157811772 37.0 35.0 37.0 32.0 37.0 8 35.202457820150855 37.0 35.0 37.0 32.0 37.0 9 36.799666419208954 39.0 37.0 39.0 33.0 39.0 10-11 36.655901281908896 39.0 37.0 39.0 32.0 39.0 12-13 36.55464860030513 39.0 37.0 39.0 32.0 39.0 14-15 37.67058253883819 40.0 37.0 41.0 32.0 41.0 16-17 37.59390221328516 40.0 37.0 41.0 32.0 41.0 18-19 37.53960648378178 40.0 37.0 41.0 32.0 41.0 20-21 37.460650716360846 40.0 37.0 41.0 32.0 41.0 22-23 37.34119021314487 40.0 37.0 41.0 32.0 41.0 24-25 37.30199232686241 40.0 37.0 41.0 31.5 41.0 26-27 37.33443013606277 40.0 37.0 41.0 31.5 41.0 28-29 37.2564885555679 40.0 37.0 41.0 31.0 41.0 30-31 37.095543617831765 39.5 36.5 41.0 31.0 41.0 32-33 36.91906047905475 39.0 36.0 41.0 30.0 41.0 34-35 36.764977524214814 39.0 36.0 41.0 30.0 41.0 36-37 36.60033026836497 39.0 36.0 41.0 30.0 41.0 38-39 36.446332657208714 39.0 35.0 41.0 30.0 41.0 40-41 36.268557867109365 39.0 35.0 41.0 30.0 41.0 42-43 36.05632040123223 39.0 35.0 40.0 29.0 41.0 44-45 35.82174218023884 39.0 35.0 40.0 28.5 41.0 46-47 35.50856944111578 38.5 35.0 40.0 27.0 41.0 48-49 35.29221540901642 38.5 35.0 40.0 26.0 41.0 50-51 34.61422082970428 38.0 34.0 39.5 25.0 40.5 52-53 34.807478873541314 38.0 34.5 40.0 26.0 40.5 54-55 35.19578503523837 39.0 35.0 40.0 26.0 41.0 56-57 35.08191376390975 39.0 35.0 40.0 26.0 41.0 58-59 34.86958413464817 38.0 34.0 40.0 26.0 41.0 60-61 34.58476030700344 38.0 34.0 40.0 25.0 41.0 62-63 34.26919697049408 38.0 34.0 40.0 24.0 41.0 64-65 33.93523797832894 37.0 33.5 40.0 23.0 41.0 66-67 33.57273834367005 37.0 33.0 39.5 22.5 41.0 68-69 33.18363544607662 36.0 33.0 39.0 22.0 41.0 70-71 32.77160588364811 36.0 32.0 39.0 21.0 40.5 72-73 32.37788546410012 35.0 32.0 38.5 20.0 40.0 74-75 31.974764963884812 35.0 32.0 37.5 19.0 39.5 76-77 31.51253694816443 35.0 31.0 37.0 18.0 39.0 78-79 31.05319697828802 35.0 31.0 36.0 14.5 39.0 80-81 30.619775183693513 35.0 31.0 36.0 9.5 37.5 82-83 30.219126143030557 34.0 30.0 35.5 7.0 37.0 84-85 29.843306463712373 34.0 30.0 35.0 6.0 37.0 86-87 29.485831194870027 34.0 29.5 35.0 2.0 36.0 88-89 29.119875530718858 34.0 29.0 35.0 2.0 36.0 90-91 28.75177419386266 34.0 29.0 35.0 2.0 36.0 92-93 28.35939257899649 34.0 29.0 35.0 2.0 35.0 94-95 27.97689407445944 34.0 28.5 35.0 2.0 35.0 96-97 27.462242239667667 34.0 27.0 35.0 2.0 35.0 98-99 26.857042510117516 34.0 25.5 35.0 2.0 35.0 100-101 24.639927204565694 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44235034625210545 1101 2 0.4027006170424201 1101 3 0.31357181180078797 1101 4 0.3785268919810818 1101 5 0.36805884884936546 1101 6 0.28324426220984833 1101 7 0.16701133996886597 1101 8 0.3323121246722138 1101 9 0.25430570778211603 1101 10-11 0.24877959211299583 1101 12-13 0.3645763004258882 1101 14-15 0.48328521015582737 1101 16-17 0.505929415917791 1101 18-19 0.5774632195649616 1101 20-21 0.5643635976594794 1101 22-23 0.695771846678717 1101 24-25 0.6183416681348035 1101 26-27 0.566596810594163 1101 28-29 0.5744253553643759 1101 30-31 0.5374366040345464 1101 32-33 0.5947666305681167 1101 34-35 0.6692412279430755 1101 36-37 0.5333173497867065 1101 38-39 0.38890138559121823 1101 40-41 0.48757924981229905 1101 42-43 0.44722842988593214 1101 44-45 0.4884370634620083 1101 46-47 0.43162861167359523 1101 48-49 0.5422450149027398 1101 50-51 0.5631638385163242 1101 52-53 0.6029900671871218 1101 54-55 0.602244103879265 1101 56-57 0.6137258847453566 1101 58-59 0.5441980311136518 1101 60-61 0.6144849027178196 1101 62-63 0.571277013930164 1101 64-65 0.4291474399502846 1101 66-67 0.4526851040707527 1101 68-69 0.5231630343202198 1101 70-71 0.385472479186582 1101 72-73 0.5360618960375234 1101 74-75 0.39203058046725303 1101 76-77 0.4171767480092754 1101 78-79 0.3808252844085054 1101 80-81 0.3905473446618295 1101 82-83 0.3864974840127715 1101 84-85 0.3686238505594339 1101 86-87 0.47295597050069205 1101 88-89 0.48430642569071125 1101 90-91 0.4797601134088083 1101 92-93 0.49588547346739986 1101 94-95 0.48610389399082266 1101 96-97 0.6248736570465105 1101 98-99 0.5760232564489272 1101 100-101 0.9183086561395619 1102 1 0.24331073255565272 1102 2 0.1777155950963163 1102 3 0.2284093650380008 1102 4 0.21419159196415194 1102 5 0.2734626702067615 1102 6 0.1441518671539086 1102 7 0.2146138745160755 1102 8 0.12951904323067254 1102 9 0.2600836548770289 1102 10-11 0.24797533574081854 1102 12-13 0.07321122989878859 1102 14-15 0.0834721102196454 1102 16-17 0.36601159984829224 1102 18-19 0.24155919632944034 1102 20-21 0.05666211027360646 1102 22-23 0.11464777629489475 1102 24-25 0.1302431157962758 1102 26-27 0.09365824666392086 1102 28-29 0.043322693829317416 1102 30-31 0.14627292321035412 1102 32-33 0.21653861574635158 1102 34-35 0.163644401336839 1102 36-37 0.17964968024165273 1102 38-39 0.24822449151862003 1102 40-41 0.16816288921890532 1102 42-43 0.18044486927690429 1102 44-45 0.2502017549792086 1102 46-47 0.2508418731122717 1102 48-49 0.3008964690763989 1102 50-51 0.31289236128947806 1102 52-53 0.43785702299535245 1102 54-55 0.18003352220979707 1102 56-57 0.4085395707792969 1102 58-59 0.14211339534085 1102 60-61 0.15608888948503363 1102 62-63 -0.004986515159821181 1102 64-65 0.30881627843476167 1102 66-67 -0.0019045298135083044 1102 68-69 0.31713379781023576 1102 70-71 0.2732372000086869 1102 72-73 0.20046394607724238 1102 74-75 0.38309177558571506 1102 76-77 0.2475197617239644 1102 78-79 0.24010443300382533 1102 80-81 0.15992031774040427 1102 82-83 0.2824337914666337 1102 84-85 0.20150702825124966 1102 86-87 0.2962796495014608 1102 88-89 0.4713280233932089 1102 90-91 0.29908326817769293 1102 92-93 0.3983205468598605 1102 94-95 0.44665015338466674 1102 96-97 0.23937314583901426 1102 98-99 0.31958432132343617 1102 100-101 0.28621931530417655 1103 1 0.20380806953951947 1103 2 0.23854097386093187 1103 3 0.15561819226573803 1103 4 0.1599076539974007 1103 5 0.10954791258271257 1103 6 0.05952716225144172 1103 7 0.15368263237501623 1103 8 0.11702851167261485 1103 9 0.008752146811474404 1103 10-11 0.11460768435618718 1103 12-13 0.15625808562182186 1103 14-15 0.4018000507163464 1103 16-17 0.38321682287252656 1103 18-19 0.30925626652682325 1103 20-21 0.3115122960867325 1103 22-23 0.30409796555598234 1103 24-25 0.3002629308343643 1103 26-27 0.29425533765685685 1103 28-29 0.43426200098181056 1103 30-31 0.4578863511042002 1103 32-33 0.38485168688330873 1103 34-35 0.3238006050384499 1103 36-37 0.3522631480026135 1103 38-39 0.3361305570321562 1103 40-41 0.4418621434881942 1103 42-43 0.3863076531012055 1103 44-45 0.3366653309740357 1103 46-47 0.41147163359705274 1103 48-49 0.39481654411279266 1103 50-51 0.37286584965205094 1103 52-53 0.2867136177180285 1103 54-55 0.2873010025967915 1103 56-57 0.28846531556720834 1103 58-59 0.40324426941138114 1103 60-61 0.3769717469449887 1103 62-63 0.32265996150228204 1103 64-65 0.34667321041175114 1103 66-67 0.2657707481519296 1103 68-69 0.34599853835606353 1103 70-71 0.2579691690243848 1103 72-73 0.16196052019831342 1103 74-75 0.3194324638646684 1103 76-77 0.28300298359097553 1103 78-79 0.22416310555789565 1103 80-81 0.30425237101581004 1103 82-83 0.42514866718356714 1103 84-85 0.45355762609648664 1103 86-87 0.5343486045410408 1103 88-89 0.3062730121496031 1103 90-91 0.42808816321402077 1103 92-93 0.3935701635428508 1103 94-95 0.5446494702561111 1103 96-97 0.5605963015266013 1103 98-99 0.5894824608217348 1103 100-101 0.6173114697774302 1104 1 0.10291781352133711 1104 2 0.07976517302959252 1104 3 0.07513178082610139 1104 4 0.1536642046307719 1104 5 0.0925745520001513 1104 6 0.09612774322304318 1104 7 0.11606073020760732 1104 8 0.19285832858044216 1104 9 0.21015091051398116 1104 10-11 0.2645578522834455 1104 12-13 0.06415119188753948 1104 14-15 0.053798112043921265 1104 16-17 0.27695898574201294 1104 18-19 0.1733207783195425 1104 20-21 0.18049910345243347 1104 22-23 0.0025249208741016105 1104 24-25 0.15709890402482074 1104 26-27 0.15700841002205834 1104 28-29 0.1660294485084748 1104 30-31 0.1795723869916941 1104 32-33 -0.05521014899536425 1104 34-35 -0.00946647806821943 1104 36-37 0.09555340906176468 1104 38-39 0.04121290335088901 1104 40-41 -0.04805565722132599 1104 42-43 0.14717172758991381 1104 44-45 0.04531258711194397 1104 46-47 -0.08156736669490527 1104 48-49 0.005509120656746802 1104 50-51 0.028125575045301332 1104 52-53 0.0890895416330082 1104 54-55 0.1968980611917459 1104 56-57 0.139599480940376 1104 58-59 0.133070681565151 1104 60-61 0.1538406334580884 1104 62-63 -0.042819959690575615 1104 64-65 -0.003276064844030202 1104 66-67 -0.15074845268654968 1104 68-69 -0.2327962606248164 1104 70-71 -0.07826079575185219 1104 72-73 -0.12871116783296088 1104 74-75 0.010658448376293705 1104 76-77 -0.023957858586804548 1104 78-79 0.044441293497801126 1104 80-81 0.04287711082832857 1104 82-83 0.1262701776257238 1104 84-85 0.08382228743792908 1104 86-87 0.1735628225923307 1104 88-89 0.07963054913246026 1104 90-91 0.14304844458407118 1104 92-93 0.15027281362193534 1104 94-95 0.32499399944016716 1104 96-97 0.3315278724813808 1104 98-99 0.28033982452130246 1104 100-101 0.3478428298517038 1105 1 0.16754251536732312 1105 2 0.13906649680441063 1105 3 0.247307844829038 1105 4 0.17313858831391116 1105 5 0.16559452179037493 1105 6 0.1421405383236376 1105 7 0.26226949842857294 1105 8 0.21360539003881485 1105 9 0.21119197649802146 1105 10-11 0.2029457524403 1105 12-13 0.26693001898221524 1105 14-15 0.2917428897783765 1105 16-17 0.3956145900878738 1105 18-19 0.3311043326161567 1105 20-21 0.24295442321650995 1105 22-23 0.17967318618619998 1105 24-25 0.15613104108413012 1105 26-27 0.17153644428161385 1105 28-29 0.2569986066813925 1105 30-31 0.29562684319728305 1105 32-33 0.13541229054683868 1105 34-35 -0.04712298089899036 1105 36-37 0.02990142450747868 1105 38-39 0.23678591303267638 1105 40-41 0.23411635489424043 1105 42-43 0.3214667888773448 1105 44-45 0.2297061071521611 1105 46-47 0.23731821391470476 1105 48-49 0.26813473312221703 1105 50-51 0.04886874553110232 1105 52-53 0.09082992750141727 1105 54-55 0.07231551638489009 1105 56-57 0.12635025768402386 1105 58-59 0.11256697076439792 1105 60-61 0.24794090342562924 1105 62-63 0.24031055065202622 1105 64-65 0.4127221239412151 1105 66-67 0.3505259397545686 1105 68-69 0.38025640916271897 1105 70-71 0.31449555866800694 1105 72-73 0.28032613696552033 1105 74-75 0.36827806742501323 1105 76-77 0.4082179389834337 1105 78-79 0.2872903453930853 1105 80-81 0.13038871120249595 1105 82-83 0.22838881878730533 1105 84-85 0.22897825080844214 1105 86-87 0.3306826692959248 1105 88-89 0.2785987951031217 1105 90-91 0.2316584863005744 1105 92-93 0.18857838343706135 1105 94-95 0.14042327527609189 1105 96-97 0.10078436170035232 1105 98-99 0.17538809368480202 1105 100-101 0.33329520883190256 1106 1 0.24166607399115847 1106 2 0.2671063931589792 1106 3 0.3122463412145322 1106 4 0.2971337097292306 1106 5 0.32183532458765285 1106 6 0.3480221297834518 1106 7 0.32657263123285674 1106 8 0.36210357386461567 1106 9 0.4028215511333002 1106 10-11 0.47658935965405647 1106 12-13 0.48942035032992237 1106 14-15 0.4037665157871402 1106 16-17 0.4702703706719191 1106 18-19 0.6027101580884917 1106 20-21 0.5412718992836645 1106 22-23 0.35602849437601947 1106 24-25 0.22955807888336466 1106 26-27 0.07450613925811211 1106 28-29 0.1343872903522012 1106 30-31 0.36591079054047526 1106 32-33 0.34972539464080654 1106 34-35 0.5436750231636864 1106 36-37 0.24398116543587633 1106 38-39 0.1333000201334329 1106 40-41 0.40244529969388054 1106 42-43 0.40512849835524634 1106 44-45 0.2527952056985967 1106 46-47 0.2901774549911025 1106 48-49 0.48183206634452347 1106 50-51 0.4678360977793261 1106 52-53 0.48569033994194655 1106 54-55 0.6065396415879007 1106 56-57 0.5241534251254194 1106 58-59 0.6368925092315365 1106 60-61 0.6101720372207495 1106 62-63 0.5487473813073365 1106 64-65 0.46477442527988444 1106 66-67 0.4383532692052725 1106 68-69 0.6951162198271774 1106 70-71 0.7149405230967361 1106 72-73 0.6273587161428011 1106 74-75 0.7813402469879982 1106 76-77 0.7267844103994179 1106 78-79 0.7383624514624181 1106 80-81 0.7466527593501802 1106 82-83 0.7056727514359658 1106 84-85 0.7432754708128826 1106 86-87 0.8303177550805039 1106 88-89 0.863058277729639 1106 90-91 0.9190582748392586 1106 92-93 1.068332886786365 1106 94-95 1.106035550948313 1106 96-97 1.153970381182507 1106 98-99 1.2875634232446735 1106 100-101 1.4429117305241803 1107 1 0.40037363335575904 1107 2 0.3768810704220549 1107 3 0.38529888892756503 1107 4 0.2225687126153275 1107 5 0.33400808852537267 1107 6 0.38234787253845326 1107 7 0.34365775159541556 1107 8 0.32835963338109053 1107 9 0.426882533944557 1107 10-11 0.49004087650097006 1107 12-13 0.5178180216426753 1107 14-15 0.5005678597267433 1107 16-17 0.5695746025591433 1107 18-19 0.6749370816685598 1107 20-21 0.6900815031599876 1107 22-23 0.786318575135077 1107 24-25 0.7259538167678414 1107 26-27 0.7439680238924566 1107 28-29 0.6159027995363502 1107 30-31 0.850132581962292 1107 32-33 0.7043845798663355 1107 34-35 0.7128737959984335 1107 36-37 0.6953402191794211 1107 38-39 0.8454793618661398 1107 40-41 0.8734955370488109 1107 42-43 0.8936237871019657 1107 44-45 0.7343616373630653 1107 46-47 0.7348403134347379 1107 48-49 0.8173121751663786 1107 50-51 0.7953762906934898 1107 52-53 0.841418010360421 1107 54-55 0.8691224830031672 1107 56-57 0.7612926903487676 1107 58-59 0.8887712087792963 1107 60-61 0.9402066925923265 1107 62-63 1.11382062025055 1107 64-65 1.0247299919051898 1107 66-67 1.035442114521409 1107 68-69 0.853984824307318 1107 70-71 0.8371951990590389 1107 72-73 0.818979713942575 1107 74-75 0.9631448835204885 1107 76-77 0.9252788344247733 1107 78-79 0.9416218597417192 1107 80-81 0.9797988595676905 1107 82-83 0.9955627544440127 1107 84-85 0.8897006609591962 1107 86-87 0.8524893586656255 1107 88-89 0.9105723516241468 1107 90-91 0.9830850531165716 1107 92-93 1.0238091967468463 1107 94-95 1.201576101576272 1107 96-97 1.075954258933077 1107 98-99 1.3405302674625332 1107 100-101 1.557819748560643 1108 1 0.11658395075813388 1108 2 0.12292468298554127 1108 3 0.16229760108439706 1108 4 0.14499524777486528 1108 5 0.12806150988983234 1108 6 0.17667830325323308 1108 7 0.1775707603689085 1108 8 0.13295109117478177 1108 9 0.003229411950449901 1108 10-11 -0.056143966331688944 1108 12-13 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-3.4810527490729086 2316 84-85 -3.40331247750866 2316 86-87 -3.73769066016256 2316 88-89 -3.450111562488516 2316 90-91 -3.800342017660263 2316 92-93 -3.858700019030156 2316 94-95 -4.031616210689624 2316 96-97 -4.395861473335792 2316 98-99 -4.75414416982732 2316 100-101 -4.870274047654323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7891.0 3 2851.0 4 2831.0 5 2944.0 6 3121.0 7 3658.0 8 4447.0 9 5312.0 10 6322.0 11 7708.0 12 9404.0 13 10991.0 14 12121.0 15 14414.0 16 17611.0 17 22476.0 18 20424.0 19 14185.0 20 14443.0 21 15477.0 22 17135.0 23 18822.0 24 21715.0 25 24974.0 26 28698.0 27 34017.0 28 40557.0 29 48981.0 30 60299.0 31 74700.0 32 94127.0 33 122401.0 34 164009.0 35 231036.0 36 343904.0 37 506023.0 38 455711.0 39 80314.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2061890031752 19.014382922156013 14.71747761866057 23.06195045600822 2 14.95462477980905 20.207711351152867 41.0575870229326 23.78007684610548 3 18.72288293457369 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22-23 22.295530840264245 26.522395306359964 29.301569412985085 21.880504440390705 24-25 22.12249593849071 26.724643408546033 29.34965380955638 21.80320684340687 26-27 22.047193309640356 26.92899374732197 29.113835996243992 21.909976946793684 28-29 21.796476659221636 26.857942014492288 29.39551435217897 21.950066974107113 30-31 22.009961986847777 26.627036182254244 29.428216561949277 21.934785268948705 32-33 21.807918279714887 26.816953038545382 29.318255201294974 22.05687348044476 34-35 22.189472486324615 26.834602271930112 29.10323922298556 21.872686018759712 36-37 22.133402495933254 26.661773061972756 29.057333508967886 22.147490933126104 38-39 21.950314146286715 26.809176298381686 29.240234419146983 22.000275136184616 40-41 22.193033975067724 26.657120999130523 29.21256816779693 21.937276858004818 42-43 22.02299333457911 26.775167385715807 28.967038054687745 22.234801225017335 44-45 22.167505442233605 26.881828122844443 28.775561740610637 22.17510469431132 46-47 22.207082238753447 27.049947916118644 28.47037843723415 22.272591407893756 48-49 22.279793654737027 27.18601762410674 28.233351356880853 22.30083736427538 50-51 22.2343875680428 27.21102075110521 28.19189316423178 22.362698516620213 52-53 22.461360448165603 27.279230328694137 28.03096659237901 22.228442630761247 54-55 22.311622870222646 27.17207425937542 28.111511832318342 22.404791038083584 56-57 22.237198656368978 27.49950017206552 28.00928569229384 22.254015479271658 58-59 22.403387278841958 27.207683225748248 28.08718084719774 22.301748648212058 60-61 22.401833804813737 27.0993998123951 28.306555922356353 22.19221046043481 62-63 22.23871860096505 27.23464008296559 28.303979565708957 22.222661750360402 64-65 22.1761544551469 27.22425119148224 28.201696280221576 22.397898073149282 66-67 22.144027191342115 27.463957147130845 27.97274239458044 22.419273266946597 68-69 22.005950641605327 27.319889579983265 28.204415436111695 22.469744342299713 70-71 22.059510183344454 27.241769697154023 28.163297693041184 22.53542242646034 72-73 22.114328270843654 27.200635854211836 28.131014962215467 22.554020912729047 74-75 22.041517326103666 27.39714014445825 27.942015587023388 22.619326942414695 76-77 22.177154996004756 27.354797216971022 27.920212040303248 22.547835746720978 78-79 22.166607092949423 27.36312434977269 27.8917062861121 22.57856227116579 80-81 22.01239368070478 27.603628407969705 27.866945374988788 22.51703253633673 82-83 22.122829092672415 27.468911305128785 27.93080772526099 22.477451876937806 84-85 22.21261513644108 27.390717450540556 27.68736789039114 22.709299522627223 86-87 22.14169355395135 27.350732203424187 27.8930776790124 22.61449656361206 88-89 22.229482516261097 27.233399761125064 27.802769693877888 22.734348028735948 90-91 22.194007238907336 27.335647018278163 27.89624518718704 22.574100555627464 92-93 22.139232445167067 27.30008461851397 27.83210330125263 22.728579635066335 94-95 22.115933437744868 27.15136379039979 27.82635570815203 22.90634706370331 96-97 22.185502672084564 27.176044215725693 27.73737393028435 22.9010791819054 98-99 22.03983473242648 27.135922774814258 27.775821011717138 23.04842148104212 100-101 21.979510085848457 27.238088887121016 27.815423084632524 22.966977942398 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 409.0 1 302.5 2 274.5 3 397.0 4 363.5 5 344.0 6 657.5 7 1447.5 8 2512.0 9 3051.0 10 2700.0 11 2311.5 12 2373.5 13 2540.5 14 2725.0 15 2908.5 16 3102.0 17 3303.0 18 3473.5 19 3770.0 20 4281.0 21 4942.0 22 6004.5 23 7476.0 24 9271.0 25 11515.5 26 14532.5 27 18090.0 28 21033.0 29 24545.5 30 29140.5 31 33858.0 32 38670.5 33 44025.5 34 50649.5 35 56028.5 36 61908.5 37 68201.5 38 72971.0 39 76118.5 40 78620.0 41 83155.0 42 86026.0 43 89488.0 44 94596.0 45 97978.0 46 99224.5 47 100911.0 48 104413.0 49 104318.0 50 101398.5 51 97582.0 52 91884.0 53 87348.0 54 83001.5 55 76433.5 56 69524.0 57 61703.5 58 54576.5 59 48572.5 60 41131.0 61 32214.5 62 24423.5 63 18761.5 64 14166.0 65 10627.0 66 7513.0 67 5425.0 68 3595.5 69 2041.0 70 1282.0 71 764.0 72 463.5 73 306.0 74 187.5 75 128.0 76 84.5 77 42.5 78 30.5 79 22.5 80 15.5 81 12.5 82 14.5 83 11.5 84 6.0 85 8.5 86 7.5 87 6.5 88 6.5 89 3.5 90 3.5 91 3.5 92 2.5 93 1.5 94 2.5 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03507274672215954 2 0.02759056075476551 3 0.02330389171094601 4 0.04504899467868493 5 0.09126708091477517 6 0.10662115003536503 7 0.053778211640644634 8 0.08047246886806607 9 0.06558603637043835 10-11 0.06732018884725624 12-13 0.06535221805895729 14-15 0.05976006344270185 16-17 0.047016965466983876 18-19 0.05633072820764625 20-21 0.05407048452999596 22-23 0.03259816959231829 24-25 0.00929427788137228 26-27 0.002903244034223207 28-29 0.0069366099072715555 30-31 0.005494730319804996 32-33 0.006683306736500402 34-35 0.006663821877210314 36-37 0.006274124691408541 38-39 0.0036241838279564864 40-41 0.007170428218752618 42-43 0.003526759531506043 44-45 0.0020653950847493957 46-47 0.0016367281803674454 48-49 2.9227288935132956E-4 50-51 0.002455092270551168 52-53 0.006566397580759871 54-55 0.01186627930766398 56-57 0.008261580338997583 58-59 0.0055142151790950845 60-61 0.0024940619891313455 62-63 0.008261580338997583 64-65 0.0026694257227421435 66-67 0.02231016388715149 68-69 0.045263328130875906 70-71 0.05617484933332554 72-73 0.003819032420857373 74-75 0.01445776559324577 76-77 0.021238496626196614 78-79 0.025174438202794518 80-81 0.07345791952363416 82-83 0.02700601497606285 84-85 0.010931006061739724 86-87 0.02946110724661402 88-89 0.044990540100814665 90-91 0.04158068972504915 92-93 0.04105459852421676 94-95 0.01116482437322079 96-97 0.015646342009941173 98-99 0.03591059567163336 100-101 0.07049622091154069 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2566095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.58807130978076 #Duplication Level Percentage of deduplicated Percentage of total 1 75.76165818638279 42.11444457811882 2 13.575262205158323 15.092452870186248 3 4.543675453162943 7.577224652967662 4 2.0431560551210692 4.543004179563214 5 1.0838958103427916 3.012583879885385 6 0.6700009126084422 2.2346435104617766 7 0.46230822516399983 1.7989175811260225 8 0.2971078875056239 1.3212523549888753 9 0.22105616411213763 1.1059277232718894 >10 1.2084026477510412 12.161128007310127 >50 0.08943310842376229 3.398012020398531 >100 0.0418636353314628 4.061969834207732 >500 0.0018281429783264926 0.6142325143680628 >1k 2.1093957441969908E-4 0.295683676178487 >5k 7.031319147323304E-5 0.2764925743934722 >10k+ 7.031319147323304E-5 0.39203004257372437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10030 0.390866277359178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7074 0.275671789236174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4670 0.18198858576942786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9484859290088637E-5 0.0 18-19 0.0 0.0 0.0 9.742429645044318E-5 0.0 20-21 0.0 0.0 0.0 1.1690915574053181E-4 0.0 22-23 0.0 0.0 0.0 1.558788743207091E-4 0.0 24-25 0.0 0.0 0.0 1.9484859290088636E-4 0.0 26-27 0.0 0.0 0.0 2.727880300612409E-4 0.0 28-29 0.0 0.0 0.0 8.768186680539886E-4 0.0 30-31 0.0 0.0 0.0 0.0019484859290088636 0.0 32-33 0.0 0.0 0.0 0.00385800213943755 0.0 34-35 0.0 0.0 0.0 0.005689578912705882 0.0 36-37 0.0 0.0 0.0 0.007949822590356164 0.0 38-39 0.0 0.0 0.0 0.010307490564456888 0.0 40-41 0.0 0.0 0.0 0.012119582478435132 0.0 42-43 0.0 0.0 0.0 0.014360341296795327 0.0 44-45 0.0 0.0 0.0 0.016815433567346494 0.0 46-47 0.0 0.0 0.0 0.0212579814854867 0.0 48-49 0.0 0.0 0.0 0.02630456004161966 0.0 50-51 0.0 0.0 0.0 0.03148753261278324 0.0 52-53 0.0 0.0 0.0 0.04185347775511039 0.0 54-55 0.0 0.0 0.0 0.06053945781430539 0.0 56-57 0.0 0.0 0.0 0.09491074960202175 0.0 58-59 0.0 0.0 0.0 0.13421171079013053 0.0 60-61 0.0 0.0 0.0 0.16951827582377116 0.0 62-63 3.8969718580177274E-5 0.0 0.0 0.21616502896424333 0.0 64-65 3.8969718580177274E-5 0.0 0.0 0.2697483920119871 0.0 66-67 3.8969718580177274E-5 0.0 0.0 0.3494219816491595 0.0 68-69 3.8969718580177274E-5 0.0 0.0 0.44624224746160995 0.0 70-71 3.8969718580177274E-5 0.0 0.0 0.5359505396331781 0.0 72-73 3.8969718580177274E-5 0.0 0.0 0.6274904085780144 0.0 74-75 3.8969718580177274E-5 0.0 0.0 0.7258304934150919 0.0 76-77 3.8969718580177274E-5 0.0 0.0 0.8425448005627227 0.0 78-79 3.8969718580177274E-5 0.0 0.0 0.9467693129054069 0.0 80-81 3.8969718580177274E-5 0.0 0.0 1.0621196799027315 0.0 82-83 3.8969718580177274E-5 0.0 0.0 1.1898819022678429 0.0 84-85 3.8969718580177274E-5 0.0 0.0 1.3593612083730338 0.0 86-87 3.8969718580177274E-5 0.0 0.0 1.5665437172045462 0.0 88-89 3.8969718580177274E-5 0.0 0.0 1.813183066098488 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4885 0.0 46.413757 1 GTATCAA 8805 0.0 43.45697 1 ATCAACG 11080 0.0 34.108505 3 TATCAAC 11280 0.0 33.71446 2 TCAACGC 11405 0.0 33.053173 4 CAACGCA 11650 0.0 32.31726 5 AACGCAG 11915 0.0 31.592947 6 ACGCAGA 13655 0.0 27.24861 7 CGCAGAG 13605 0.0 27.103725 8 GTACATG 9520 0.0 25.014608 1 GCAGAGT 14640 0.0 24.083063 9 TACATGG 9755 0.0 23.683413 2 ACATGGG 9355 0.0 23.374878 3 GAGTACT 8050 0.0 21.368513 12-13 AGAGTAC 13115 0.0 20.88698 10-11 AGTACTT 8510 0.0 20.185537 12-13 GTACTTT 8735 0.0 19.47576 14-15 GTGGTAT 2820 0.0 19.383923 1 CAGAGTA 14135 0.0 19.228477 10-11 CATGGGG 4980 0.0 18.900406 4 >>END_MODULE