FastQCFastQC Report
Thu 26 May 2016
SRR936788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566095
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97740.38089002940265265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77760.3030285316794585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.21390478528659304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53000.047.505711
GTATCAA94400.040.711991
ATCAACG116050.032.6621173
TCAACGC117700.032.2439654
TATCAAC119100.031.78582
CAACGCA120300.031.4286385
AACGCAG124850.030.2862116
ACGCAGA144900.025.9315537
CGCAGAG144300.025.6797648
CCGTGCG1401.7783985E-523.753789
GCAGAGT158000.023.1824879
GTACATG88250.022.8242721
GAGTACT90950.021.59762212-13
TACATGG92900.020.8608042
AGTACTT95650.020.66053212-13
ACATGGG88800.020.5936993
AGAGTAC138550.020.40162310-11
GTACTTT97100.020.3748814-15
CAGAGTA152000.019.2526910-11
TACTTTT111950.017.62975114-15
Produced by FastQC (version 0.11.5)