FastQCFastQC Report
Thu 26 May 2016
SRR936787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692500
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71131.0271480144404332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.6912635379061371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.4843321299638989No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.15971119133574008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.1407942238267148No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.1267870036101083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.057.0885961
TATAACG558.1377584E-751.8625562
GTATCAA40700.045.4348561
TATCAAC54150.034.152032
ATCAACG54150.034.1470873
TCAACGC54950.033.4793624
CAACGCA55200.033.2392125
AACGCAG56700.032.359876
GGTACTC1401.692024E-830.5574443
ACGCAGA62650.028.9072277
CGCAGAG63050.028.7259128
GCAGAGT65650.027.0089729
ATAACGC908.906858E-426.4076653
ATTCCGT1109.4255825E-525.9275256
GTACATG36600.025.7168661
ACATGGG36600.024.805893
TATTCCG1351.3343462E-524.6471525
TACATGG37550.024.5616262
GAGTACT40350.023.61622612-13
GTGGTAT10200.022.8365171
Produced by FastQC (version 0.11.5)