##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936787_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 692500 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18059927797834 33.0 31.0 34.0 31.0 34.0 2 32.2833083032491 34.0 31.0 34.0 31.0 34.0 3 32.28304259927798 34.0 31.0 34.0 31.0 34.0 4 35.84426570397112 37.0 35.0 37.0 35.0 37.0 5 35.87311335740072 37.0 35.0 37.0 35.0 37.0 6 35.95356389891697 37.0 36.0 37.0 35.0 37.0 7 35.90558122743683 37.0 36.0 37.0 35.0 37.0 8 35.88247220216606 37.0 36.0 37.0 35.0 37.0 9 37.568004332129966 39.0 38.0 39.0 35.0 39.0 10-11 37.48001444043321 39.0 37.0 39.0 35.0 39.0 12-13 37.400253429602884 39.0 37.0 39.0 35.0 39.0 14-15 38.63451696750903 40.0 38.0 41.0 35.0 41.0 16-17 38.581114801444045 40.0 38.0 41.0 35.0 41.0 18-19 38.586612274368235 40.0 38.0 41.0 35.0 41.0 20-21 38.57499205776173 40.0 38.0 41.0 35.0 41.0 22-23 38.46164043321299 40.0 38.0 41.0 34.0 41.0 24-25 38.32240144404332 40.0 38.0 41.0 34.0 41.0 26-27 38.25703465703971 40.0 38.0 41.0 34.0 41.0 28-29 38.29449314079422 40.0 38.0 41.0 34.0 41.0 30-31 38.10842671480144 40.0 38.0 41.0 34.0 41.0 32-33 37.882069314079416 40.0 37.0 41.0 34.0 41.0 34-35 37.681291696750904 40.0 37.0 41.0 33.0 41.0 36-37 37.5331155234657 40.0 37.0 41.0 33.0 41.0 38-39 37.371631768953065 40.0 37.0 41.0 33.0 41.0 40-41 37.16285415162454 40.0 36.0 41.0 32.5 41.0 42-43 36.94841083032491 40.0 36.0 41.0 32.0 41.0 44-45 36.642836101083034 39.0 36.0 41.0 31.0 41.0 46-47 36.22204332129964 39.0 36.0 41.0 30.0 41.0 48-49 35.81097906137184 39.0 35.0 41.0 28.5 41.0 50-51 35.14226498194946 38.5 35.0 39.5 27.5 40.5 52-53 35.44538411552347 39.0 35.5 40.0 28.0 41.0 54-55 35.78152129963899 39.0 36.0 41.0 28.5 41.0 56-57 35.685929963898914 39.0 35.5 41.0 28.0 41.0 58-59 35.48453501805054 39.0 35.0 41.0 27.5 41.0 60-61 35.219031046931406 39.0 35.0 41.0 27.0 41.0 62-63 34.967054873646205 38.5 35.0 40.5 26.0 41.0 64-65 34.66700794223827 38.0 35.0 40.0 26.0 41.0 66-67 34.292948736462094 37.5 34.0 40.0 25.5 41.0 68-69 33.881680866425995 37.0 34.0 40.0 24.0 41.0 70-71 33.52700216606498 36.5 34.0 39.0 23.5 41.0 72-73 33.15248880866426 36.0 34.0 39.0 22.5 40.5 74-75 32.72026931407942 35.5 33.0 38.5 21.5 40.0 76-77 32.283354512635384 35.0 33.0 37.5 20.0 39.5 78-79 31.851137184115522 35.0 32.5 37.0 18.0 39.0 80-81 31.41694512635379 35.0 32.0 36.5 15.5 39.0 82-83 30.999708303249097 35.0 32.0 36.0 9.5 37.5 84-85 30.6434916967509 35.0 31.0 35.5 7.0 37.0 86-87 30.307151624548737 35.0 31.0 35.0 2.0 37.0 88-89 29.937178339350183 34.0 31.0 35.0 2.0 36.0 90-91 29.60151985559567 34.0 31.0 35.0 2.0 36.0 92-93 29.255729241877255 34.0 30.0 35.0 2.0 36.0 94-95 28.895503249097473 34.0 30.0 35.0 2.0 35.0 96-97 28.483005054151626 34.0 29.5 35.0 2.0 35.0 98-99 27.974829602888086 34.0 29.0 35.0 2.0 35.0 100-101 26.07537039711191 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6004106304831716 1101 2 0.5884407981842656 1101 3 0.6219281117336308 1101 4 0.5146825414727303 1101 5 0.4749740910741451 1101 6 0.48133919452239127 1101 7 0.503485605611445 1101 8 0.4179464667840165 1101 9 0.5915086731842791 1101 10-11 0.5937778145420367 1101 12-13 0.7502949577600404 1101 14-15 0.9128784713841185 1101 16-17 0.9362985659684071 1101 18-19 0.9888013767323258 1101 20-21 0.9732243122255966 1101 22-23 0.9651044975742948 1101 24-25 1.12358536302132 1101 26-27 1.0333945721483886 1101 28-29 1.0161199925242812 1101 30-31 0.9978547892259684 1101 32-33 1.144294422477408 1101 34-35 1.16748816937519 1101 36-37 1.2133707453198568 1101 38-39 1.2386034994512087 1101 40-41 1.4446310278866505 1101 42-43 1.4277347761747379 1101 44-45 1.474464876273892 1101 46-47 1.5822920036563772 1101 48-49 1.6998313318359521 1101 50-51 1.6860055119700235 1101 52-53 1.7468073043149417 1101 54-55 1.8057432595089864 1101 56-57 1.6729726202405928 1101 58-59 1.7368112092554284 1101 60-61 1.9499153645495468 1101 62-63 2.041006849991817 1101 64-65 1.9378251661056751 1101 66-67 1.8404450459734676 1101 68-69 1.9817440506369906 1101 70-71 1.8928855955921904 1101 72-73 1.8499198262847898 1101 74-75 1.8865151319471352 1101 76-77 1.9262115435348015 1101 78-79 1.9274795874858874 1101 80-81 1.8823060215543528 1101 82-83 1.9062241932255723 1101 84-85 2.0340660566828497 1101 86-87 2.144241047965817 1101 88-89 2.0486342787811793 1101 90-91 2.109349871114972 1101 92-93 2.0969152989450492 1101 94-95 2.3496185447830022 1101 96-97 2.258845711529265 1101 98-99 2.2566945455021106 1101 100-101 2.274273982643013 1102 1 0.4588817926270856 1102 2 0.42336844085328806 1102 3 0.4157815392332225 1102 4 0.35776952676651774 1102 5 0.3735598087452914 1102 6 0.37655744023997073 1102 7 0.4259599737004436 1102 8 0.3859222276929444 1102 9 0.5709858179704881 1102 10-11 0.60272383215014 1102 12-13 0.5227481708592521 1102 14-15 0.6773935330661374 1102 16-17 0.5684820403722668 1102 18-19 0.5814265572623469 1102 20-21 0.5483780422107927 1102 22-23 0.5668668451577688 1102 24-25 0.6083491593163757 1102 26-27 0.6135169301500625 1102 28-29 0.722742063242876 1102 30-31 0.6973329288835615 1102 32-33 0.8525708860561778 1102 34-35 0.722074889742558 1102 36-37 0.8100883125128036 1102 38-39 0.8273467253831441 1102 40-41 0.9255933535606005 1102 42-43 1.0044839333286433 1102 44-45 1.253227977276815 1102 46-47 1.2366242213923613 1102 48-49 1.472512023953442 1102 50-51 1.211545481207267 1102 52-53 1.3127148785278706 1102 54-55 1.3728198075959241 1102 56-57 1.412404766658831 1102 58-59 1.2584817995159696 1102 60-61 1.3130576713991786 1102 62-63 1.3716969274352948 1102 64-65 1.4337656157875358 1102 66-67 1.3219446301395834 1102 68-69 1.4158360572366746 1102 70-71 1.430728574706606 1102 72-73 1.4861003920871454 1102 74-75 1.4335272760850017 1102 76-77 1.4005789338865604 1102 78-79 1.4842660634949532 1102 80-81 1.4721624694387394 1102 82-83 1.557582835416163 1102 84-85 1.626565760457101 1102 86-87 1.5203762073911626 1102 88-89 1.5910012678308867 1102 90-91 1.5587651326655383 1102 92-93 1.6845718514586103 1102 94-95 1.7196278395645095 1102 96-97 1.5624527909893082 1102 98-99 1.3989524899413368 1102 100-101 1.665069941229536 1103 1 0.5402499915372481 1103 2 0.4819230196042952 1103 3 0.5230389909611262 1103 4 0.46938720947805024 1103 5 0.4084567073230403 1103 6 0.4955332923948248 1103 7 0.4268634229974566 1103 8 0.3969613123495179 1103 9 0.5888322974876914 1103 10-11 0.6378745588959873 1103 12-13 0.4506050394332135 1103 14-15 0.7503894701883453 1103 16-17 0.8363264860099164 1103 18-19 0.780331028380445 1103 20-21 0.6696100769527149 1103 22-23 0.6694982378445857 1103 24-25 0.8944964870427157 1103 26-27 0.8652764393505166 1103 28-29 0.7295087805124609 1103 30-31 0.7910335564574353 1103 32-33 0.8023112816701641 1103 34-35 0.853467373174567 1103 36-37 0.993331911080297 1103 38-39 0.7642991033137676 1103 40-41 0.8752997833860618 1103 42-43 0.9870007046994331 1103 44-45 1.0313209385891682 1103 46-47 1.2434653786535534 1103 48-49 1.2687903091163761 1103 50-51 1.2547381677018166 1103 52-53 1.2502402003282924 1103 54-55 1.1896854283603702 1103 56-57 1.3421659566573538 1103 58-59 1.1444875285478915 1103 60-61 1.2353498455187975 1103 62-63 1.2762931291245536 1103 64-65 1.357093237987094 1103 66-67 1.3767086675694742 1103 68-69 1.3206877141976605 1103 70-71 1.3479561853537234 1103 72-73 1.4952168504031889 1103 74-75 1.5087936329511322 1103 76-77 1.4618103015159924 1103 78-79 1.4736040601073306 1103 80-81 1.462129138220643 1103 82-83 1.5595961727847119 1103 84-85 1.7661387108353246 1103 86-87 1.6908816183243225 1103 88-89 1.916380708400112 1103 90-91 1.7822933767441 1103 92-93 1.842490395396105 1103 94-95 2.041561074620784 1103 96-97 2.1712515166417354 1103 98-99 2.3232641030681584 1103 100-101 2.4470221889918626 1104 1 0.32053216420449715 1104 2 0.5149770871264039 1104 3 0.3890754927675246 1104 4 0.41435731038748 1104 5 0.35606789456609533 1104 6 0.4750742167750417 1104 7 0.4539220999626963 1104 8 0.32191770950910836 1104 9 0.3320769717121834 1104 10-11 0.4037444355622313 1104 12-13 0.4301926811424508 1104 14-15 0.5627415319797962 1104 16-17 0.3494961010591311 1104 18-19 0.4973750672492656 1104 20-21 0.4559250140400408 1104 22-23 0.4713887324253747 1104 24-25 0.28573231611957794 1104 26-27 0.31217752319255254 1104 28-29 0.4995567436369157 1104 30-31 0.6300783428035146 1104 32-33 0.5492263807108984 1104 34-35 0.7081827089164392 1104 36-37 0.659050735049405 1104 38-39 0.6732470290643562 1104 40-41 0.6286026985473896 1104 42-43 0.6634224687457717 1104 44-45 0.6596518841479053 1104 46-47 0.8723039858542592 1104 48-49 0.7436968744583439 1104 50-51 0.9232615886560893 1104 52-53 1.128669252277959 1104 54-55 1.0395951081909374 1104 56-57 0.9902162867931423 1104 58-59 1.0962129552328364 1104 60-61 1.0127277575656422 1104 62-63 1.0501111807004833 1104 64-65 1.0518250805185474 1104 66-67 0.8066466892463211 1104 68-69 0.7587810242761464 1104 70-71 0.8722227232881892 1104 72-73 0.9386437222108412 1104 74-75 0.6520320781984168 1104 76-77 0.793660619193826 1104 78-79 0.9034766775235532 1104 80-81 1.0753221119343586 1104 82-83 1.1198646417021187 1104 84-85 1.1913473815715854 1104 86-87 1.3590048274933757 1104 88-89 1.3257054872714207 1104 90-91 1.4111942737168164 1104 92-93 1.0925589141453251 1104 94-95 1.2497490651527379 1104 96-97 1.0305591658257391 1104 98-99 1.089179139819123 1104 100-101 1.4229409631291468 1105 1 0.20775904568761305 1105 2 0.22349066028906606 1105 3 0.0902396949304034 1105 4 0.20826042228330977 1105 5 0.09466312740652683 1105 6 0.2672668894742287 1105 7 0.2821388291615463 1105 8 0.25827281875651664 1105 9 0.36135858724959036 1105 10-11 0.2821846253658151 1105 12-13 0.290168403940946 1105 14-15 0.3947322625028633 1105 16-17 0.25271558903087765 1105 18-19 0.26166275208704803 1105 20-21 0.2525482760131226 1105 22-23 0.23883521223115878 1105 24-25 0.3842260261173678 1105 26-27 0.3519424757416587 1105 28-29 0.4132843975764473 1105 30-31 0.18127289146826797 1105 32-33 0.4428287863608631 1105 34-35 0.20850824411839142 1105 36-37 0.14789425745063767 1105 38-39 0.36294025351811143 1105 40-41 0.5849506349852618 1105 42-43 0.5701952562099066 1105 44-45 0.6203752344017133 1105 46-47 0.8485371573395781 1105 48-49 0.8330038707064134 1105 50-51 0.7431495824764411 1105 52-53 0.6370669569789129 1105 54-55 0.9831671673390332 1105 56-57 1.015083865694045 1105 58-59 1.0264435886470906 1105 60-61 0.9107276472147205 1105 62-63 0.6760629573502257 1105 64-65 1.0851869211718181 1105 66-67 1.018142495911512 1105 68-69 1.0866942884562434 1105 70-71 0.9292217301299459 1105 72-73 0.8750457305975132 1105 74-75 0.7834434664129475 1105 76-77 0.7235243358126731 1105 78-79 0.8568782445065573 1105 80-81 0.8020270335852082 1105 82-83 1.057030830181482 1105 84-85 1.1118781694771265 1105 86-87 1.3893347767319568 1105 88-89 1.216496703338514 1105 90-91 1.043160175283802 1105 92-93 1.1782684705346433 1105 94-95 1.210822779564566 1105 96-97 1.3509382212218988 1105 98-99 1.5359348228912921 1105 100-101 1.7778350262498712 1106 1 0.4390485638993873 1106 2 0.32563688880451735 1106 3 0.5029389557728692 1106 4 0.43863267270632633 1106 5 0.5016628602411544 1106 6 0.5545364593379745 1106 7 0.5500700786049535 1106 8 0.6259413555808067 1106 9 0.618518619309441 1106 10-11 0.8338455034493464 1106 12-13 0.6951621700821846 1106 14-15 0.7843662459386138 1106 16-17 0.82912043362969 1106 18-19 0.8034296057694803 1106 20-21 0.7786748233459164 1106 22-23 0.6197106241111143 1106 24-25 0.4949237730227054 1106 26-27 0.4714833965716494 1106 28-29 0.28710441716857105 1106 30-31 0.5564799446341766 1106 32-33 0.7267721473012827 1106 34-35 0.7417258057890592 1106 36-37 0.789008782341547 1106 38-39 0.6810273494304866 1106 40-41 0.7511141401949928 1106 42-43 1.0285731171058003 1106 44-45 1.1839939894110643 1106 46-47 1.0792166479442642 1106 48-49 1.1481209781959478 1106 50-51 0.7091216191671492 1106 52-53 1.0350765749324253 1106 54-55 1.0418834377103963 1106 56-57 1.1552811227961968 1106 58-59 1.3064150910070467 1106 60-61 1.4564062472681485 1106 62-63 1.3748918824548682 1106 64-65 1.591899451227924 1106 66-67 1.8236661400472371 1106 68-69 1.8256002462441074 1106 70-71 1.896908077979262 1106 72-73 1.8367653850636216 1106 74-75 1.8554645724776009 1106 76-77 1.7589638228551472 1106 78-79 1.7706105448004372 1106 80-81 1.7575684887459566 1106 82-83 1.6764626584831994 1106 84-85 1.8912329650956146 1106 86-87 2.176110090651271 1106 88-89 1.9176009868900366 1106 90-91 1.8438125040753235 1106 92-93 1.6894315304687453 1106 94-95 1.4260267158011004 1106 96-97 1.493631248205599 1106 98-99 1.4113377973324717 1106 100-101 1.4446419547386071 1107 1 0.1321309534174837 1107 2 0.13615210088534724 1107 3 0.2969318493993356 1107 4 0.03019385414092568 1107 5 0.04843168102729578 1107 6 0.09834058991759065 1107 7 0.30191329423897173 1107 8 0.05974104467696861 1107 9 0.21222281651130004 1107 10-11 0.16130497491280948 1107 12-13 0.20879752429049603 1107 14-15 -0.029374593501763968 1107 16-17 0.4189994032055253 1107 18-19 0.013012106324666206 1107 20-21 0.3285859936768034 1107 22-23 0.042439915694508556 1107 24-25 0.02619736840355813 1107 26-27 0.22947251493718568 1107 28-29 0.07671689773708579 1107 30-31 0.3350109239812795 1107 32-33 0.3585343058622428 1107 34-35 0.31148485531160475 1107 36-37 0.6197827108336682 1107 38-39 0.8267968364391507 1107 40-41 0.853279363379535 1107 42-43 0.9658371632972305 1107 44-45 0.936075934780817 1107 46-47 1.028089621517438 1107 48-49 0.7871338584979668 1107 50-51 0.38632570533192734 1107 52-53 0.22228510258065626 1107 54-55 0.2667002265178553 1107 56-57 0.3528882735845613 1107 58-59 0.4379163124149983 1107 60-61 0.4920824631242482 1107 62-63 0.5018516870295997 1107 64-65 0.31483416142113185 1107 66-67 0.540005247309054 1107 68-69 0.5679719984093339 1107 70-71 0.4319846935688716 1107 72-73 0.7098944101580074 1107 74-75 0.7442720341698035 1107 76-77 0.31539287015695194 1107 78-79 0.28455125099630507 1107 80-81 0.10113388958983549 1107 82-83 0.5176907286586392 1107 84-85 0.35059339147802504 1107 86-87 0.07830862496173197 1107 88-89 0.22050460669905192 1107 90-91 0.2102793039864963 1107 92-93 0.24718191909854426 1107 94-95 0.3737171444042531 1107 96-97 0.40189240743278276 1107 98-99 0.43872509468979004 1107 100-101 0.4464962740812659 1108 1 -0.07798800342253287 1108 2 -0.1186137981538451 1108 3 -0.08563639827490377 1108 4 -0.21538339155070219 1108 5 -0.08543289118560438 1108 6 -0.16042866431453007 1108 7 -0.0304414411583096 1108 8 -0.40274179808649535 1108 9 -0.3575118182301935 1108 10-11 -0.7303375445320555 1108 12-13 -0.42211219051811355 1108 14-15 -0.15609031715587207 1108 16-17 -0.3001175710204933 1108 18-19 0.014537194017208321 1108 20-21 -0.2170713191186806 1108 22-23 -0.18068041372443844 1108 24-25 0.0231471930184739 1108 26-27 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-1.2227883937223432 2316 80-81 -1.030806861394101 2316 82-83 -1.3016370024337967 2316 84-85 -0.9464494763019964 2316 86-87 -0.8578018951956992 2316 88-89 -0.8348352507629215 2316 90-91 -0.840792390514089 2316 92-93 -0.9654151450435648 2316 94-95 -1.1486342011972823 2316 96-97 -0.7206876372433122 2316 98-99 -0.8041347074589176 2316 100-101 -0.6303838450549968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1944.0 3 483.0 4 454.0 5 460.0 6 430.0 7 543.0 8 633.0 9 756.0 10 932.0 11 1308.0 12 1742.0 13 2511.0 14 2764.0 15 3750.0 16 5474.0 17 8497.0 18 7512.0 19 4023.0 20 3242.0 21 3219.0 22 3297.0 23 3349.0 24 3751.0 25 4247.0 26 4895.0 27 5708.0 28 6856.0 29 8320.0 30 10677.0 31 13716.0 32 18614.0 33 25703.0 34 37996.0 35 59055.0 36 93735.0 37 150204.0 38 157075.0 39 34591.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19938276688614 19.8879964630462 15.481108072143455 21.43151269792421 2 14.550218668794098 21.01503944131785 42.1657893900109 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46-47 20.328067991090904 27.450651860539686 31.415474790777754 20.805805357591655 48-49 20.347142022428738 27.814913442002997 30.95099320146501 20.88695133410325 50-51 20.484206421422073 27.844144027037565 30.72612909282609 20.94552045871427 52-53 20.53100042322876 28.019982753066962 30.50952120260524 20.939495621099034 54-55 20.35873519625057 27.978282898685546 30.50188114242296 21.16110076264092 56-57 20.406547696005813 28.337210822492377 30.20743423376312 21.048807247738694 58-59 20.493966095720467 27.902590746929956 30.38708433164046 21.21635882570912 60-61 20.56969134014557 27.8109513466896 30.729471010185133 20.889886302979697 62-63 20.423952921087057 27.709352578266817 30.915798118894916 20.950896381751217 64-65 20.35074567038834 27.70944241376347 30.728452332201595 21.2113595836466 66-67 20.33953279386035 28.05172814838252 30.361897661946642 21.24684139581049 68-69 20.228251134032533 27.90362890410563 30.531911819941637 21.3362081419202 70-71 20.328866839260375 27.890998768735265 30.437225357554226 21.342909034450138 72-73 20.43951060540304 27.78880614205673 30.288854885100008 21.482828367440224 74-75 20.30568663220398 28.116049414813176 30.066888810892866 21.511375142089975 76-77 20.305294109574497 27.945519721545885 30.22684707342212 21.522339095457497 78-79 20.372043966205407 27.847238025060594 30.13527229645041 21.645445712283593 80-81 20.163518832833773 28.01783973132065 30.104871618937562 21.71376981690802 82-83 20.306469953345363 28.121446539335683 29.95411114177432 21.61797236554463 84-85 20.258531630499014 27.970122857790987 29.833001252707337 21.93834425900266 86-87 20.233323051072016 27.88361443398319 30.07294144673584 21.810121068208957 88-89 20.503010228708014 27.748802700679505 29.85184414443317 21.896342926179308 90-91 20.384961045866472 27.93673132051045 29.888122457000183 21.790185176622895 92-93 20.476256533192302 28.040454133630426 29.565214878266232 21.91807445491104 94-95 20.55127360371764 27.897903625739108 29.442946483790234 22.10787628675302 96-97 20.778722435603285 27.96557318408302 29.11580750177951 22.139896878534188 98-99 20.584304004638113 28.036298154947403 29.02616240192985 22.35323543848463 100-101 20.726742547018013 27.99792685589046 29.03558319105165 22.239747406039875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 736.0 1 513.5 2 366.5 3 443.0 4 356.5 5 323.5 6 600.5 7 1373.5 8 2505.5 9 2897.5 10 2168.0 11 1538.5 12 1412.0 13 1435.0 14 1528.0 15 1585.0 16 1620.0 17 1689.0 18 1756.0 19 1814.5 20 2011.0 21 2327.0 22 2667.0 23 3141.0 24 3700.5 25 4495.0 26 5798.5 27 7013.0 28 7854.0 29 8898.5 30 10267.5 31 11936.5 32 13851.5 33 15640.0 34 17927.0 35 19725.5 36 21655.0 37 23691.5 38 24048.5 39 23974.5 40 23972.5 41 24062.0 42 24132.0 43 24898.0 44 25811.0 45 25929.0 46 25137.0 47 24175.5 48 24422.0 49 23936.5 50 22393.0 51 21096.0 52 19994.0 53 19232.0 54 17819.0 55 15485.0 56 13348.5 57 11705.0 58 10404.0 59 9049.0 60 7527.0 61 5896.5 62 4385.0 63 3245.5 64 2307.5 65 1646.5 66 1187.0 67 839.5 68 534.5 69 324.0 70 216.0 71 132.5 72 91.0 73 66.0 74 47.5 75 30.0 76 25.5 77 16.0 78 7.5 79 9.5 80 8.5 81 5.0 82 6.5 83 5.0 84 2.5 85 3.5 86 3.0 87 1.5 88 1.5 89 1.0 90 2.0 91 2.0 92 1.0 93 1.0 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05472924187725632 2 0.08462093862815884 3 0.06700361010830325 4 0.12548736462093862 5 0.10974729241877257 6 0.10483754512635379 7 0.10541516245487365 8 0.09732851985559567 9 0.11711191335740073 10-11 0.10866425992779785 12-13 0.08888086642599279 14-15 0.09407942238267147 16-17 0.10231046931407943 18-19 0.07790613718411553 20-21 0.06151624548736462 22-23 0.06433212996389892 24-25 0.05155234657039711 26-27 0.047653429602888084 28-29 0.06093862815884476 30-31 0.04223826714801444 32-33 0.044693140794223825 34-35 0.037689530685920576 36-37 0.04122743682310469 38-39 0.03942238267148015 40-41 0.04953068592057761 42-43 0.04433212996389892 44-45 0.03393501805054152 46-47 0.025126353790613717 48-49 0.03155234657039711 50-51 0.018772563176895306 52-53 0.029314079422382674 54-55 0.03386281588447654 56-57 0.03703971119133574 58-59 0.04137184115523466 60-61 0.03833935018050542 62-63 0.04339350180505415 64-65 0.029386281588447655 66-67 0.04953068592057761 68-69 0.0404332129963899 70-71 0.017689530685920576 72-73 0.013501805054151625 74-75 0.022671480144404333 76-77 0.03602888086642599 78-79 0.05451263537906137 80-81 0.032490974729241874 82-83 0.05675090252707581 84-85 0.0576173285198556 86-87 0.05335740072202166 88-89 0.075956678700361 90-91 0.05833935018050542 92-93 0.0659927797833935 94-95 0.06469314079422382 96-97 0.08628158844765343 98-99 0.12122743682310469 100-101 0.11509025270758123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 692500.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.99214733974459 #Duplication Level Percentage of deduplicated Percentage of total 1 83.97819060948012 53.739447468070075 2 9.6435393737126 12.342215849584896 3 2.6018352374625566 4.9949107160832975 4 1.1247096930722142 2.8789035357406423 5 0.6250330523649088 1.999860358957277 6 0.3741029620015677 1.4363791118783513 7 0.2627242152460358 1.1768600684220163 8 0.17846130699811774 0.9136097801493553 9 0.15325354554730433 0.882632112030122 >10 0.9646404899110634 11.54183592656324 >50 0.0675973764176098 2.9331096417497973 >100 0.024109554288105125 2.3825788047031637 >500 9.012917489530113E-4 0.4187248153084056 >1k 6.759688117147585E-4 1.3333155534286592 >5k 2.2532293723825282E-4 1.0256162573308156 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7113 1.0271480144404332 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4787 0.6912635379061371 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3354 0.4843321299638989 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1106 0.15971119133574008 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 975 0.1407942238267148 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.1267870036101083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.888086642599278E-4 0.0 16-17 0.0 0.0 0.0 4.332129963898917E-4 0.0 18-19 0.0 0.0 0.0 4.332129963898917E-4 0.0 20-21 0.0 0.0 0.0 7.220216606498195E-4 0.0 22-23 0.0 0.0 0.0 0.0010108303249097472 0.0 24-25 0.0 0.0 0.0 0.001588447653429603 0.0 26-27 0.0 0.0 0.0 0.0022382671480144405 0.0 28-29 0.0 0.0 0.0 0.005487364620938628 0.0 30-31 0.0 0.0 0.0 0.01335740072202166 0.0 32-33 0.0 0.0 0.0 0.024693140794223828 0.0 34-35 0.0 0.0 0.0 0.04310469314079422 0.0 36-37 0.0 0.0 0.0 0.07140794223826716 0.0 38-39 0.0 0.0 0.0 0.11718411552346572 0.0 40-41 0.0 0.0 0.0 0.16902527075812274 0.0 42-43 0.0 0.0 0.0 0.2311191335740072 0.0 44-45 0.0 0.0 0.0 0.30259927797833935 0.0 46-47 0.0 0.0 0.0 0.39906137184115525 0.0 48-49 0.0 0.0 0.0 0.5078700361010831 0.0 50-51 0.0 0.0 0.0 0.6224548736462094 0.0 52-53 0.0 0.0 0.0 0.7378339350180505 0.0 54-55 0.0 0.0 0.0 0.8651985559566787 0.0 56-57 0.0 0.0 0.0 1.032274368231047 0.0 58-59 0.0 0.0 0.0 1.2072202166064983 0.0 60-61 0.0 0.0 0.0 1.4012996389891699 0.0 62-63 0.0 0.0 0.0 1.6234657039711191 0.0 64-65 0.0 0.0 0.0 1.844476534296029 0.0 66-67 0.0 0.0 0.0 2.083826714801444 0.0 68-69 0.0 0.0 0.0 2.3812996389891694 0.0 70-71 0.0 0.0 0.0 2.7384115523465704 0.0 72-73 0.0 0.0 0.0 3.0902527075812274 0.0 74-75 0.0 0.0 0.0 3.4501083032490976 0.0 76-77 0.0 0.0 0.0 3.8794945848375453 0.0 78-79 0.0 0.0 0.0 4.3210108303249095 0.0 80-81 0.0 0.0 0.0 4.791552346570397 0.0 82-83 0.0 0.0 0.0 5.26173285198556 0.0 84-85 0.0 0.0 0.0 5.756462093862816 0.0 86-87 0.0 0.0 0.0 6.2316245487364625 0.0 88-89 0.0 0.0 0.0 6.718772563176895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2165 0.0 57.088596 1 TATAACG 55 8.1377584E-7 51.862556 2 GTATCAA 4070 0.0 45.434856 1 TATCAAC 5415 0.0 34.15203 2 ATCAACG 5415 0.0 34.147087 3 TCAACGC 5495 0.0 33.479362 4 CAACGCA 5520 0.0 33.239212 5 AACGCAG 5670 0.0 32.35987 6 GGTACTC 140 1.692024E-8 30.557444 3 ACGCAGA 6265 0.0 28.907227 7 CGCAGAG 6305 0.0 28.725912 8 GCAGAGT 6565 0.0 27.008972 9 ATAACGC 90 8.906858E-4 26.407665 3 ATTCCGT 110 9.4255825E-5 25.927525 6 GTACATG 3660 0.0 25.716866 1 ACATGGG 3660 0.0 24.80589 3 TATTCCG 135 1.3343462E-5 24.647152 5 TACATGG 3755 0.0 24.561626 2 GAGTACT 4035 0.0 23.616226 12-13 GTGGTAT 1020 0.0 22.836517 1 >>END_MODULE