FastQCFastQC Report
Thu 26 May 2016
SRR936787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692500
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53600.7740072202166065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41890.6049097472924188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.43725631768953066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.14974729241877255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.13097472924187725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.12259927797833935No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.11870036101083034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.049.9629861
GTATCAA44400.039.3778721
TATCAAC58450.029.822462
ATCAACG59850.029.2042183
TCAACGC60600.028.9211564
CAACGCA61050.028.630185
AACGCAG62300.028.0557386
ACGCAGA70750.024.5706467
CGCAGAG70450.024.40568
GCAGAGT72950.022.722819
AGTACTT44750.022.44808412-13
CGCAGAA1302.9413242E-421.9214789
GAGTACT43150.021.84950612-13
TGGTATC13400.021.2671072
GTGGTAT13700.021.1542021
AGAGTAC66600.020.71733310-11
GTATTAC1404.8420025E-420.361541
GTACTTT46650.020.31275614-15
GTACATG35850.019.8787691
GTGTCGG600.00476599619.79022264-65
Produced by FastQC (version 0.11.5)