FastQCFastQC Report
Thu 26 May 2016
SRR936786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433598
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47021.0844145960082843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.7433152366938962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.5205282312187787No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.16881996688176606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.1512922107574297No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.14644901498623147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.058.400991
GTATCAA28000.045.4948351
TATCAAC35700.035.544982
ATCAACG36150.035.233983
CCTAGAC958.645584E-735.0194743
TCAACGC36550.034.848384
CAACGCA37200.034.2434235
AACGCAG38300.033.1358266
GGATAGG804.471446E-429.7074491
ACGCAGA42500.029.52237
GTACATG23850.029.4956971
CATGGGG7150.029.2470364
CGCAGAG42300.029.2124638
TACATGG24350.028.6915232
GTGGTAT6500.027.7878911
ATGGGAG5650.027.7620035
ACATGGG24350.027.715623
GCAGAGT44500.027.5546519
TAGGCCC700.00836317527.157964
TGGTATC6950.025.3018422
Produced by FastQC (version 0.11.5)