##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936786_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 433598 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.143879814943794 33.0 31.0 34.0 31.0 34.0 2 32.26865898827947 33.0 31.0 34.0 31.0 34.0 3 32.26782180729616 34.0 31.0 34.0 31.0 34.0 4 35.84003385624472 37.0 35.0 37.0 35.0 37.0 5 35.85767462027039 37.0 35.0 37.0 35.0 37.0 6 35.93383041434693 37.0 36.0 37.0 35.0 37.0 7 35.89186988869875 37.0 36.0 37.0 35.0 37.0 8 35.85481482848168 37.0 36.0 37.0 35.0 37.0 9 37.55705053990101 39.0 38.0 39.0 35.0 39.0 10-11 37.42494545639048 39.0 37.0 39.0 35.0 39.0 12-13 37.38315328945244 39.0 37.0 39.0 35.0 39.0 14-15 38.60983445495597 40.0 38.0 41.0 34.5 41.0 16-17 38.56881835248318 40.0 38.0 41.0 35.0 41.0 18-19 38.60051476252197 40.0 38.0 41.0 35.0 41.0 20-21 38.573835672673766 40.0 38.0 41.0 35.0 41.0 22-23 38.47894362981379 40.0 38.0 41.0 34.0 41.0 24-25 38.34686506856581 40.0 38.0 41.0 34.0 41.0 26-27 38.38886710732061 40.0 38.0 41.0 34.0 41.0 28-29 38.30992186310823 40.0 38.0 41.0 34.0 41.0 30-31 38.12703471879483 40.0 38.0 41.0 34.0 41.0 32-33 37.873579905811376 40.0 37.0 41.0 34.0 41.0 34-35 37.694342455454134 40.0 37.0 41.0 33.0 41.0 36-37 37.51974409476058 40.0 37.0 41.0 33.0 41.0 38-39 37.366541127957234 40.0 37.0 41.0 33.0 41.0 40-41 37.17300010608905 40.0 36.0 41.0 33.0 41.0 42-43 36.92330799496308 40.0 36.0 41.0 32.0 41.0 44-45 36.59878043717914 39.5 36.0 41.0 31.0 41.0 46-47 36.13889132329946 39.0 36.0 41.0 30.0 41.0 48-49 35.71906696986609 39.0 35.0 41.0 28.5 41.0 50-51 35.05811834925438 38.5 35.0 40.0 27.0 40.5 52-53 35.255209894879584 39.0 35.0 40.0 27.5 40.5 54-55 35.64813260208765 39.0 36.0 41.0 28.0 41.0 56-57 35.56480311256048 39.0 35.0 41.0 27.5 41.0 58-59 35.35067850866471 39.0 35.0 41.0 26.5 41.0 60-61 35.107704601958496 39.0 35.0 41.0 26.0 41.0 62-63 34.82780478692245 39.0 35.0 40.5 26.0 41.0 64-65 34.532424042546324 38.0 35.0 40.0 25.5 41.0 66-67 34.159416095092695 37.5 34.0 40.0 23.5 41.0 68-69 33.7812951166749 37.0 34.0 40.0 22.0 41.0 70-71 33.37144428710465 36.5 34.0 39.0 21.5 41.0 72-73 32.98069294600067 36.0 34.0 39.0 20.0 41.0 74-75 32.55402815511142 35.5 33.0 38.5 18.0 40.0 76-77 32.11048713324323 35.0 33.0 37.5 15.0 39.5 78-79 31.658501653605413 35.0 32.5 37.0 9.0 39.0 80-81 31.207678540952678 35.0 32.0 36.5 6.5 39.0 82-83 30.785461418179974 35.0 32.0 36.0 2.0 37.5 84-85 30.42452455961513 35.0 31.0 36.0 2.0 37.0 86-87 30.080440869192202 35.0 31.0 35.0 2.0 37.0 88-89 29.711320162915882 34.0 31.0 35.0 2.0 36.0 90-91 29.35695390661396 34.0 30.5 35.0 2.0 36.0 92-93 28.993755736880704 34.0 30.0 35.0 2.0 36.0 94-95 28.602446275121196 34.0 29.5 35.0 2.0 35.0 96-97 28.144043561086537 34.0 29.0 35.0 2.0 35.0 98-99 27.60787757323604 34.0 29.0 35.0 2.0 35.0 100-101 25.716985548826333 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5187117739733687 1101 2 0.5587960130556446 1101 3 0.5167711434726314 1101 4 0.4618048567169808 1101 5 0.40853461200742913 1101 6 0.2937754247307467 1101 7 0.36026516365659234 1101 8 0.26072619785643525 1101 9 0.34146458224525134 1101 10-11 0.534516777605333 1101 12-13 0.42002068518228697 1101 14-15 0.7746634074596059 1101 16-17 0.8117036923635439 1101 18-19 0.6418045621664348 1101 20-21 0.7773339801847641 1101 22-23 0.7841787769923059 1101 24-25 0.7628024599909935 1101 26-27 0.7817242719512976 1101 28-29 0.8522118663226195 1101 30-31 0.719531827293217 1101 32-33 1.0332985428585104 1101 34-35 0.9700973226268488 1101 36-37 0.9169392225225934 1101 38-39 1.0164776483753712 1101 40-41 1.039386386490861 1101 42-43 0.9779001319403946 1101 44-45 0.9151328228539484 1101 46-47 1.1678973972690798 1101 48-49 1.3912544142457932 1101 50-51 1.3359735070027554 1101 52-53 1.5594347650305593 1101 54-55 1.6397504499711673 1101 56-57 1.667604839468524 1101 58-59 1.5343648879918632 1101 60-61 1.6066615140021057 1101 62-63 1.718880157146117 1101 64-65 1.608306588663929 1101 66-67 1.6717890527532404 1101 68-69 1.7020172584829893 1101 70-71 1.7187564904081185 1101 72-73 1.7428294568979545 1101 74-75 1.6732561468168683 1101 76-77 1.6825176920055505 1101 78-79 1.8199419623930133 1101 80-81 1.576362431092246 1101 82-83 1.5796514393569119 1101 84-85 1.7718077544960202 1101 86-87 1.6622657606808495 1101 88-89 1.8081124249080354 1101 90-91 2.036843724694595 1101 92-93 2.234101736155626 1101 94-95 2.299750623302561 1101 96-97 2.4256186371097606 1101 98-99 2.471637924583863 1101 100-101 2.6611429044512356 1102 1 0.5177714294311251 1102 2 0.5071252088729956 1102 3 0.42958089296485014 1102 4 0.4075718017256307 1102 5 0.36842477976489363 1102 6 0.3030645242568113 1102 7 0.4165519311281898 1102 8 0.3611339312499524 1102 9 0.47429690000647895 1102 10-11 0.5868480797944571 1102 12-13 0.501562097955933 1102 14-15 0.5868547863808473 1102 16-17 0.5979513415021884 1102 18-19 0.6534580640095129 1102 20-21 0.6024945910325741 1102 22-23 0.6132497165845692 1102 24-25 0.6501852930610283 1102 26-27 0.772664616493536 1102 28-29 0.7593742826319527 1102 30-31 0.8193023832249082 1102 32-33 0.8308762153176943 1102 34-35 0.8034441969215891 1102 36-37 0.6050322037909268 1102 38-39 0.8102254855829258 1102 40-41 0.7447463503816323 1102 42-43 0.7710679646342911 1102 44-45 0.817406199819267 1102 46-47 0.9054585196643288 1102 48-49 1.0778405874196437 1102 50-51 1.0653859797327598 1102 52-53 1.2264497353156827 1102 54-55 1.0915190499862106 1102 56-57 1.2335237441551996 1102 58-59 1.4113038784379555 1102 60-61 1.239324569745726 1102 62-63 1.270692389147925 1102 64-65 1.3022898881448555 1102 66-67 1.2569022702361252 1102 68-69 1.1291548496964126 1102 70-71 1.1673550009023685 1102 72-73 1.432924995903452 1102 74-75 1.334125777449536 1102 76-77 1.360633993818528 1102 78-79 1.446537915150106 1102 80-81 1.4758034902963395 1102 82-83 1.3252950111616215 1102 84-85 1.568184795043674 1102 86-87 1.625389209112356 1102 88-89 1.5312734871212292 1102 90-91 1.6344296721857567 1102 92-93 1.9092751356892173 1102 94-95 1.9416471101753494 1102 96-97 2.0781921720529652 1102 98-99 2.1625564531327264 1102 100-101 2.1411105565989814 1103 1 0.5166043942776817 1103 2 0.5612908412751985 1103 3 0.5397257284735062 1103 4 0.5952441390478569 1103 5 0.4566470571478902 1103 6 0.4605151730052057 1103 7 0.4512676190439464 1103 8 0.5652313503031223 1103 9 0.5018891515857788 1103 10-11 0.6933927128759123 1103 12-13 0.5418036444073877 1103 14-15 0.7316566213103144 1103 16-17 0.857684010597211 1103 18-19 0.8849606049327079 1103 20-21 0.8431453213676008 1103 22-23 0.8950045218253635 1103 24-25 0.7402067095511455 1103 26-27 0.9596822403599319 1103 28-29 0.891029413641995 1103 30-31 0.839836140654775 1103 32-33 0.9569823972366365 1103 34-35 0.991103064753176 1103 36-37 0.8107296059110283 1103 38-39 0.9024073121613085 1103 40-41 0.8397865952954504 1103 42-43 0.8501983358342144 1103 44-45 1.055391895220204 1103 46-47 1.4098187862063085 1103 48-49 1.3914079242896804 1103 50-51 1.1631177175087188 1103 52-53 1.2752126588109007 1103 54-55 1.2842405220860869 1103 56-57 1.2954949463842027 1103 58-59 1.3454914661099124 1103 60-61 1.3928618504095098 1103 62-63 1.4811839678299492 1103 64-65 1.3600576471872117 1103 66-67 1.5337027081869934 1103 68-69 1.6843624434104 1103 70-71 1.5574920625907112 1103 72-73 1.3928176633051699 1103 74-75 1.3366426479865652 1103 76-77 1.4737459656942384 1103 78-79 1.5134581930834194 1103 80-81 1.429294782270638 1103 82-83 1.5371604778890955 1103 84-85 1.630790025439314 1103 86-87 1.49986798792985 1103 88-89 1.712676738656267 1103 90-91 1.8666788077330878 1103 92-93 1.8725871295009462 1103 94-95 2.021659251959054 1103 96-97 2.1240750298170674 1103 98-99 2.1455002295545746 1103 100-101 2.301207479205214 1104 1 0.4779567332183241 1104 2 0.22874420300383846 1104 3 0.316925583627075 1104 4 0.2627050976172214 1104 5 0.3412713047441187 1104 6 0.27470404565936235 1104 7 0.21411197750340705 1104 8 0.30732620445644443 1104 9 0.324107884888555 1104 10-11 0.5158532789418331 1104 12-13 0.3390148441764538 1104 14-15 0.585990998787203 1104 16-17 0.6322376328974855 1104 18-19 0.6323289326908039 1104 20-21 0.6709768538276393 1104 22-23 0.6322842450977717 1104 24-25 0.7543570915456286 1104 26-27 0.5472332974603304 1104 28-29 0.4877713818821334 1104 30-31 0.7858453936067846 1104 32-33 0.6856662952262624 1104 34-35 0.7243058168353471 1104 36-37 0.659513225096596 1104 38-39 0.8419351938329172 1104 40-41 0.8569985041029753 1104 42-43 0.8236309176711814 1104 44-45 0.6986961664172924 1104 46-47 0.6786026079742911 1104 48-49 0.48501872801010393 1104 50-51 0.41366152469076667 1104 52-53 0.487362032957833 1104 54-55 0.36791465813537627 1104 56-57 0.3505062023698855 1104 58-59 0.40130545493242664 1104 60-61 0.5648517921923784 1104 62-63 0.7521620104279663 1104 64-65 0.9315755719329104 1104 66-67 0.4804007413649245 1104 68-69 0.48473646120219627 1104 70-71 0.27692300857464147 1104 72-73 0.7338613679298653 1104 74-75 0.587364980925706 1104 76-77 0.5367136462015019 1104 78-79 0.8682091306601798 1104 80-81 0.8303940451238603 1104 82-83 0.8228498025552788 1104 84-85 0.7135310162192816 1104 86-87 0.7839467823618698 1104 88-89 0.8609316177272248 1104 90-91 0.76930123715211 1104 92-93 0.893332835386726 1104 94-95 1.005986969538906 1104 96-97 1.1428202343113583 1104 98-99 1.2857315786775203 1104 100-101 1.527630050938381 1105 1 0.3611795787053218 1105 2 0.3594009846040116 1105 3 0.13912514167569157 1105 4 0.02869314662413558 1105 5 0.057938625185023795 1105 6 -0.009746587847871524 1105 7 0.17639574167396432 1105 8 -0.009845829696722319 1105 9 0.28920941602782335 1105 10-11 0.1522077030321114 1105 12-13 0.13060469237007055 1105 14-15 0.030377693173896603 1105 16-17 0.16781929583763855 1105 18-19 -0.28484114013964756 1105 20-21 -0.06692178501440083 1105 22-23 0.03921621335049963 1105 24-25 0.14586325663669442 1105 26-27 0.0361747062130533 1105 28-29 -0.013562317564769444 1105 30-31 -0.5307676635723126 1105 32-33 -0.4057999896362503 1105 34-35 -0.12390088042796066 1105 36-37 -0.45267443426087084 1105 38-39 -0.36090480145991677 1105 40-41 -0.1988436224938681 1105 42-43 -0.4539387419739498 1105 44-45 -0.551725517966652 1105 46-47 -0.4425548339378693 1105 48-49 -0.2073020397823626 1105 50-51 -0.18818342621079154 1105 52-53 -0.20763920921321954 1105 54-55 -0.04996802861523264 1105 56-57 0.18466982898633688 1105 58-59 0.18327174633267873 1105 60-61 0.2168052696175593 1105 62-63 0.1672988453761235 1105 64-65 0.30387711876276313 1105 66-67 0.20740750139055564 1105 68-69 0.476162212061908 1105 70-71 0.10197302230390193 1105 72-73 0.3611642876856145 1105 74-75 0.656370851818366 1105 76-77 0.7100259280043524 1105 78-79 0.9219395664660901 1105 80-81 1.041759256489069 1105 82-83 0.8604450901505629 1105 84-85 0.4444702282905695 1105 86-87 0.437917153313375 1105 88-89 0.17874435063429672 1105 90-91 0.2922585195433527 1105 92-93 0.05480853459827628 1105 94-95 -0.4198390742116658 1105 96-97 -0.1332522823271951 1105 98-99 0.018654995563199606 1105 100-101 -0.0031038760219601613 1106 1 -0.08486131495537208 1106 2 -0.04820670993034071 1106 3 -0.20212903584595665 1106 4 0.0880863204879816 1106 5 -0.1150305072062281 1106 6 -0.09378385002518286 1106 7 -0.06908464042124507 1106 8 -0.2056827813558897 1106 9 0.10813523376945966 1106 10-11 -0.22636786076884619 1106 12-13 -0.14803271190876188 1106 14-15 -0.07527084239095672 1106 16-17 0.21783771640551208 1106 18-19 -0.025736116587637525 1106 20-21 -0.23706862325549594 1106 22-23 -0.35800359665450543 1106 24-25 -0.3239664846859043 1106 26-27 -0.8733079389804956 1106 28-29 -0.8343565071913588 1106 30-31 -0.3422002574514167 1106 32-33 -0.3110064315719896 1106 34-35 -0.3195141537628743 1106 36-37 -0.8052046308346519 1106 38-39 -0.6754623131043402 1106 40-41 -0.3936082339514613 1106 42-43 -0.2699633202745204 1106 44-45 -0.6456309415081378 1106 46-47 -0.9403180506953177 1106 48-49 -0.8239305495584119 1106 50-51 -0.5699891681649447 1106 52-53 -0.3272544824955048 1106 54-55 -0.4651084196575752 1106 56-57 -0.3431715436091167 1106 58-59 -0.5155388112842232 1106 60-61 -0.7101505999648197 1106 62-63 -0.8990875655077986 1106 64-65 -0.6345784481960024 1106 66-67 -0.6066302870886986 1106 68-69 -1.0375019020403542 1106 70-71 -1.266148869225269 1106 72-73 -1.0286017711023092 1106 74-75 -0.6567524870411354 1106 76-77 -0.564091858369693 1106 78-79 -0.520055381035295 1106 80-81 -0.8634011340269687 1106 82-83 -0.9592899168229359 1106 84-85 -1.0791080985955652 1106 86-87 -0.9331915017220176 1106 88-89 -1.0340526810227644 1106 90-91 -1.116105734028924 1106 92-93 -1.3171422035401967 1106 94-95 -1.3437029198999362 1106 96-97 -1.093220441059863 1106 98-99 -0.8682624539776036 1106 100-101 -0.9626838870743022 1107 1 0.08109354879037411 1107 2 -0.08588529297281156 1107 3 -0.02182249087229593 1107 4 0.1699255903299388 1107 5 0.14553272040450338 1107 6 0.009561262381865276 1107 7 -0.15545487911008138 1107 8 0.13656796115934355 1107 9 -0.010363693106341998 1107 10-11 0.1503420921871239 1107 12-13 0.182858089574097 1107 14-15 0.25188028384747696 1107 16-17 0.20486266925308882 1107 18-19 0.3769456099499706 1107 20-21 0.355480209315445 1107 22-23 -0.07926229546431074 1107 24-25 -0.21772718882881037 1107 26-27 0.07937703944862307 1107 28-29 0.17790312382734896 1107 30-31 0.486576814545451 1107 32-33 0.45344822622062964 1107 34-35 0.6397938437223374 1107 36-37 0.7062853858583935 1107 38-39 0.6122108501189345 1107 40-41 0.9096306499993716 1107 42-43 0.9979708614411749 1107 44-45 0.5459379877523745 1107 46-47 0.7717052762064966 1107 48-49 1.166031945292758 1107 50-51 1.0003817642607444 1107 52-53 1.0618560167782434 1107 54-55 0.9883136541820434 1107 56-57 1.3946284433833185 1107 58-59 1.4057112158148684 1107 60-61 0.8585835470640362 1107 62-63 1.4229364117723193 1107 64-65 1.3633078223180135 1107 66-67 1.3836140383606121 1107 68-69 1.2776172255855514 1107 70-71 1.3987762288734586 1107 72-73 1.907910466849433 1107 74-75 1.9005133225714559 1107 76-77 1.781464973854387 1107 78-79 1.584883158215039 1107 80-81 1.4009993255737 1107 82-83 1.6812568345374395 1107 84-85 1.6740299767182414 1107 86-87 1.1049748650479856 1107 88-89 1.4125404206313625 1107 90-91 1.3026945347941101 1107 92-93 1.547329797673843 1107 94-95 1.0082167800588735 1107 96-97 1.0395326102983447 1107 98-99 1.1278135969253427 1107 100-101 1.6363036907001067 1108 1 0.10543963780966337 1108 2 0.16147721405009818 1108 3 -0.25145605101961976 1108 4 0.05718447040984387 1108 5 0.10760900481675861 1108 6 -0.02293140257849302 1108 7 0.28858032908019027 1108 8 0.3318985061371791 1108 9 -0.19196054684253028 1108 10-11 0.2744135073815812 1108 12-13 0.5383109892557272 1108 14-15 0.19302829558353807 1108 16-17 0.31758818270586175 1108 18-19 0.17579385266174796 1108 20-21 0.11493772851139994 1108 22-23 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-1.4803672228227605 2316 92-93 -1.4200955490722968 2316 94-95 -1.4104620485814188 2316 96-97 -1.3441324350925647 2316 98-99 -1.0636797568062377 2316 100-101 -1.288198741213531 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1261.0 3 306.0 4 285.0 5 293.0 6 307.0 7 315.0 8 397.0 9 504.0 10 632.0 11 828.0 12 1163.0 13 1645.0 14 1872.0 15 2688.0 16 3755.0 17 5635.0 18 5095.0 19 2669.0 20 2259.0 21 2136.0 22 2249.0 23 2348.0 24 2526.0 25 2735.0 26 3181.0 27 3538.0 28 4324.0 29 5349.0 30 6629.0 31 8663.0 32 11385.0 33 15607.0 34 22990.0 35 35836.0 36 56650.0 37 92440.0 38 100216.0 39 22866.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27620972393332 19.86637320450529 15.62732151179155 21.230095559769843 2 14.568088071091752 20.90004337113697 42.52862956435077 22.003238993420506 3 17.831628239582013 27.154242742298244 31.61785907890336 23.39626993921639 4 11.633900369846547 17.825080810201584 39.80545564033289 30.735563179618985 5 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20.190553509279738 27.829876446968143 31.121318145020858 20.858251898731258 52-53 20.253817057982378 28.000138382766732 30.850477420545232 20.89556713870566 54-55 20.048457088031146 28.036496190433358 30.876459795723736 21.03858692581176 56-57 20.17251381390698 28.250146183486514 30.641096762511516 20.93624324009499 58-59 20.254516309455852 27.767072024540585 30.822536283277692 21.15587538272587 60-61 20.162060423211592 27.7096567345263 31.161686756992403 20.96659608526971 62-63 20.082480028692718 27.62688858161337 31.2932826982225 20.997348691471412 64-65 20.036349839527915 27.568447139929585 31.17886727349679 21.21633574704571 66-67 20.061931287409614 27.838863324453055 30.758283453851387 21.340921934285944 68-69 19.926163512872947 27.591341224449312 30.944777181503742 21.537718081174003 70-71 19.991096615560625 27.543519327955934 30.92922732001209 21.536156736471355 72-73 20.1892358828509 27.33148341648733 30.74218110270729 21.737099597954483 74-75 19.975664890549673 27.702927133070375 30.595806518580027 21.72560145779992 76-77 19.94763763861897 27.459791127232464 30.73382274071981 21.858748493428752 78-79 20.028791351520947 27.378775836950513 30.750916743855015 21.84151606767352 80-81 19.827606093881204 27.62789399378062 30.65441861752683 21.890081294811342 82-83 19.922490455483917 27.706547941729433 30.375321514665682 21.99564008812097 84-85 19.872352302110315 27.514606596438135 30.28149846495224 22.331542636499307 86-87 19.926992566620726 27.542025408432185 30.35113922736683 22.17984279758026 88-89 20.219101680802133 27.292333171104133 30.221915703863285 22.266649444230445 90-91 20.119688933329183 27.55290451526443 30.149317076489968 22.178089474916415 92-93 20.26974031756138 27.785928658265107 29.67051219056451 22.273818833609003 94-95 20.380132977343106 27.534959893433598 29.638723740434685 22.446183388788615 96-97 20.703811707816747 27.727740429203564 29.159149529131117 22.409298333848575 98-99 20.695467771821775 27.756753576101328 28.767338236074607 22.78044041600229 100-101 21.034309279682823 27.54688765655181 28.843495498738815 22.575307565026552 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 434.0 1 304.0 2 236.5 3 286.0 4 221.0 5 189.0 6 376.5 7 895.0 8 1638.5 9 1856.0 10 1384.5 11 1000.0 12 906.5 13 930.5 14 1009.5 15 1041.0 16 1069.5 17 1150.0 18 1178.5 19 1227.5 20 1353.5 21 1546.5 22 1786.5 23 2033.0 24 2414.0 25 2969.0 26 3771.0 27 4583.5 28 5096.0 29 5700.0 30 6594.0 31 7546.5 32 8629.5 33 9796.5 34 11248.0 35 12342.5 36 13526.0 37 14849.5 38 14992.0 39 14889.0 40 15002.0 41 15175.5 42 15212.5 43 15611.5 44 16151.5 45 16228.5 46 15792.5 47 15386.5 48 15646.5 49 15124.0 50 14011.0 51 13254.5 52 12482.0 53 11898.0 54 10968.5 55 9493.0 56 8167.5 57 7050.0 58 6202.5 59 5371.0 60 4411.5 61 3449.5 62 2619.5 63 1878.5 64 1312.0 65 971.5 66 653.5 67 450.0 68 305.5 69 182.5 70 111.0 71 61.0 72 40.5 73 34.5 74 24.5 75 18.5 76 19.0 77 10.0 78 5.0 79 5.5 80 2.5 81 1.5 82 2.0 83 1.5 84 3.0 85 3.5 86 1.5 87 0.5 88 1.0 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03505551224867273 2 0.029981688107417474 3 0.021448438415306345 4 0.04035996475998506 5 0.062269659915405506 6 0.06480657198603315 7 0.06457594361597609 8 0.06918851101711723 9 0.06388405850580492 10-11 0.07091822379254517 12-13 0.06815068335186047 14-15 0.06284623084054816 16-17 0.05027698467243853 18-19 0.03874556616958565 20-21 0.04958509956226735 22-23 0.024792549781133676 24-25 0.012107989427995517 26-27 0.010493590837596115 28-29 0.01176204687290993 30-31 0.00807199295199701 32-33 0.0046125674011411495 34-35 0.008763878062168183 36-37 0.004958509956226736 38-39 0.005535080881369379 40-41 0.006111651806512022 42-43 0.003228797180798804 44-45 6.918851101711724E-4 46-47 0.0019603411454849886 48-49 8.07199295199701E-4 50-51 0.0043819390310840915 52-53 0.004151310661027034 54-55 0.004266624846055563 56-57 0.015567414978851377 58-59 0.007034165286740252 60-61 0.012915188723195217 62-63 0.008879192247196712 64-65 0.007726050396911425 66-67 0.012223303613024044 68-69 0.016720556829136667 70-71 0.01268456035313816 72-73 0.014990844053708737 74-75 0.01568272916387991 76-77 0.018565583789593124 78-79 0.031019515772674228 80-81 0.026291634186504553 82-83 0.023754722115876918 84-85 0.014068330573480505 86-87 0.018565583789593124 88-89 0.01268456035313816 90-91 0.013261131278280804 92-93 0.024677235596105147 94-95 0.015106158238737264 96-97 0.033210485288216275 98-99 0.054197666963408504 100-101 0.0634228017656908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 433598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.38960563404932 #Duplication Level Percentage of deduplicated Percentage of total 1 86.41804482391267 59.100960051729835 2 8.184351742097443 11.194491760247773 3 2.126967850212866 4.363874774170787 4 0.9553045758528254 2.613316128119099 5 0.5185635891137744 1.7732179677834112 6 0.3375774040686205 1.3852071321131443 7 0.24579615359898566 1.1766931407700587 8 0.17800842407631884 0.9739140737694444 9 0.13482640289185868 0.8298652070548495 >10 0.8436883268016867 10.467657514585373 >50 0.04139110658410349 1.9097013517751675 >100 0.01346052242512003 1.4022427462449158 >500 0.0010095391818840023 0.46557312910774085 >1k 0.0010095391818840023 2.343285022528432 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4702 1.0844145960082843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3223 0.7433152366938962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2257 0.5205282312187787 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.16881996688176606 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 656 0.1512922107574297 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 635 0.14644901498623147 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.3062837005705745E-4 0.0 10-11 0.0 0.0 0.0 2.3062837005705745E-4 0.0 12-13 0.0 0.0 0.0 2.3062837005705745E-4 0.0 14-15 0.0 0.0 0.0 2.3062837005705745E-4 0.0 16-17 0.0 0.0 0.0 2.3062837005705745E-4 0.0 18-19 0.0 0.0 0.0 4.612567401141149E-4 0.0 20-21 0.0 0.0 0.0 6.918851101711724E-4 0.0 22-23 0.0 0.0 0.0 0.002075655330513517 0.0 24-25 0.0 0.0 0.0 0.003344111365827333 0.0 26-27 0.0 0.0 0.0 0.0043819390310840915 0.0 28-29 0.0 0.0 0.0 0.00818730713702554 0.0 30-31 0.0 0.0 0.0 0.01994935400993547 0.0 32-33 0.0 0.0 0.0 0.0393221370947283 0.0 34-35 0.0 0.0 0.0 0.0723019940128875 0.0 36-37 0.0 0.0 0.0 0.1232708637954972 0.0 38-39 0.0 0.0 0.0 0.20352953657535322 0.0 40-41 0.0 0.0 0.0 0.3118095563171417 0.0 42-43 0.0 0.0 0.0 0.4234336874247575 0.0 44-45 0.0 0.0 0.0 0.5588125406482503 0.0 46-47 0.0 0.0 0.0 0.7299387912305868 0.0 48-49 0.0 0.0 0.0 0.9165171426067464 0.0 50-51 0.0 0.0 0.0 1.1231601621778697 0.0 52-53 0.0 0.0 0.0 1.3195402192814543 0.0 54-55 0.0 0.0 0.0 1.5290660934782911 0.0 56-57 0.0 0.0 0.0 1.8052435666216173 0.0 58-59 0.0 0.0 0.0 2.0992947384443656 0.0 60-61 0.0 0.0 0.0 2.427478909035558 0.0 62-63 0.0 0.0 0.0 2.793601446501137 0.0 64-65 0.0 0.0 0.0 3.160646497446944 0.0 66-67 0.0 0.0 0.0 3.5547903818744553 0.0 68-69 0.0 0.0 0.0 4.047527894501359 0.0 70-71 0.0 0.0 0.0 4.653503936826277 0.0 72-73 0.0 0.0 0.0 5.260402492631424 0.0 74-75 0.0 0.0 0.0 5.825441999271215 0.0 76-77 0.0 0.0 0.0 6.5311648116458105 0.0 78-79 0.0 0.0 0.0 7.264101771687139 0.0 80-81 0.0 0.0 0.0 8.01491243040789 0.0 82-83 0.0 0.0 0.0 8.737817056351737 0.0 84-85 0.0 0.0 0.0 9.497968164059799 0.0 86-87 0.0 0.0 0.0 10.191006416081255 0.0 88-89 0.0 0.0 0.0 10.898228312861221 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1465 0.0 58.40099 1 GTATCAA 2800 0.0 45.494835 1 TATCAAC 3570 0.0 35.54498 2 ATCAACG 3615 0.0 35.23398 3 CCTAGAC 95 8.645584E-7 35.019474 3 TCAACGC 3655 0.0 34.84838 4 CAACGCA 3720 0.0 34.243423 5 AACGCAG 3830 0.0 33.135826 6 GGATAGG 80 4.471446E-4 29.707449 1 ACGCAGA 4250 0.0 29.5223 7 GTACATG 2385 0.0 29.495697 1 CATGGGG 715 0.0 29.247036 4 CGCAGAG 4230 0.0 29.212463 8 TACATGG 2435 0.0 28.691523 2 GTGGTAT 650 0.0 27.787891 1 ATGGGAG 565 0.0 27.762003 5 ACATGGG 2435 0.0 27.71562 3 GCAGAGT 4450 0.0 27.554651 9 TAGGCCC 70 0.008363175 27.15796 4 TGGTATC 695 0.0 25.301842 2 >>END_MODULE