FastQCFastQC Report
Thu 26 May 2016
SRR936786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433598
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.803047984538674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.6303073353659381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.43957767332875153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.1750469328733066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.15083095401731558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.11969612405961282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.10885659066693111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.049.4291041
GTATCAA28750.039.9883881
TAGGGCG300.003949858231.6675930-31
ATCAACG36700.031.189413
TCAACGC37100.030.7251154
TATCAAC37400.030.4786572
CAACGCA37850.030.1162935
AACGCAG39050.029.1908256
AGCCGTA700.00838943327.1405167
CCATACG458.944245E-426.38965862-63
ACAGCCG908.943024E-426.3866145
TACACTC908.943024E-426.3866145
GGTAAGG1457.8586345E-726.2106841
CGCAGAG43450.026.0161698
ACGCAGA43500.025.877087
GTTATAT1551.4054822E-624.5196721
GCAGAGT44950.024.3026879
GAGTACT25900.023.83964312-13
CAGCCGT1000.001652819623.7479536
TAAGGGT1452.3315606E-522.9290583
Produced by FastQC (version 0.11.5)