##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936786_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 433598 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61362598535971 31.0 31.0 34.0 30.0 34.0 2 31.697800727863136 31.0 31.0 34.0 30.0 34.0 3 31.807298465398826 31.0 31.0 34.0 30.0 34.0 4 35.63600846867375 37.0 35.0 37.0 33.0 37.0 5 35.51402220489947 37.0 35.0 37.0 33.0 37.0 6 35.55075669168216 37.0 35.0 37.0 33.0 37.0 7 35.48217473327829 37.0 35.0 37.0 33.0 37.0 8 35.45090844514966 37.0 35.0 37.0 33.0 37.0 9 37.02098026282409 39.0 37.0 39.0 33.0 39.0 10-11 36.90139714666581 39.0 37.0 39.0 33.0 39.0 12-13 36.788817060964305 39.0 37.0 39.0 32.0 39.0 14-15 37.78800640224355 39.5 37.0 41.0 32.5 41.0 16-17 37.718771765552425 39.0 37.0 41.0 32.0 41.0 18-19 37.79420799911439 39.5 37.0 41.0 32.5 41.0 20-21 37.88431219701198 40.0 37.0 41.0 33.0 41.0 22-23 37.80122025470597 39.5 37.0 41.0 32.0 41.0 24-25 37.69124050387686 39.0 37.0 41.0 32.0 41.0 26-27 37.43113552184282 39.0 36.0 41.0 32.0 41.0 28-29 37.22546344770963 39.0 36.0 40.5 31.5 41.0 30-31 37.1135960036716 39.0 36.0 40.0 31.0 41.0 32-33 36.94846609071075 39.0 35.5 40.0 31.0 41.0 34-35 36.77550288516091 39.0 35.0 40.0 31.0 41.0 36-37 36.53797411427175 38.5 35.0 40.0 30.0 41.0 38-39 36.3359009958533 38.0 35.0 40.0 30.0 41.0 40-41 36.0739579057099 38.0 35.0 40.0 30.0 41.0 42-43 35.77069774307077 38.0 35.0 40.0 29.0 41.0 44-45 35.39944949008067 38.0 34.5 40.0 27.5 41.0 46-47 34.927644961462 38.0 34.0 40.0 25.5 41.0 48-49 34.44156107731124 38.0 33.5 40.0 24.0 41.0 50-51 34.15874957910323 38.0 33.0 40.0 23.0 41.0 52-53 33.88447824943842 38.0 33.0 40.0 22.5 41.0 54-55 33.54179216693804 38.0 33.0 40.0 21.0 41.0 56-57 33.24833716945189 37.0 32.5 40.0 19.5 41.0 58-59 32.848489845432866 37.0 32.0 39.5 18.0 41.0 60-61 32.460981139211896 36.0 31.0 39.0 15.0 40.0 62-63 32.070267851788984 36.0 31.0 39.0 13.0 40.0 64-65 31.61717535597489 35.5 30.5 39.0 8.5 40.0 66-67 31.118480712549413 35.0 29.5 38.0 8.0 40.0 68-69 30.60711419333115 34.5 29.0 38.0 7.0 40.0 70-71 30.17454762245213 34.0 29.0 37.0 3.5 39.0 72-73 29.730906277242976 34.0 28.0 37.0 2.0 39.0 74-75 29.19074004031384 34.0 28.0 36.0 2.0 39.0 76-77 27.88697018897689 31.5 26.0 34.5 2.0 37.0 78-79 28.544246052795447 33.0 27.5 35.0 2.0 37.5 80-81 28.098256910779106 33.0 26.0 35.0 2.0 37.0 82-83 27.92526372354116 33.0 26.0 35.0 2.0 37.0 84-85 27.52200425278714 33.0 26.0 35.0 2.0 36.0 86-87 27.06136329042108 33.0 26.0 35.0 2.0 36.0 88-89 26.56134599329333 32.0 25.0 34.5 2.0 35.0 90-91 25.954917919363098 32.0 23.5 34.0 2.0 35.0 92-93 25.339275088907236 31.5 20.5 34.0 2.0 35.0 94-95 24.590443221601575 31.0 18.5 34.0 2.0 35.0 96-97 23.677513503291067 31.0 4.5 34.0 2.0 35.0 98-99 22.582955871567673 30.5 2.0 34.0 2.0 35.0 100-101 19.452167676050166 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.505930642840557 1101 2 0.4581280281813136 1101 3 0.5294427766810621 1101 4 0.49873854886266855 1101 5 0.5704952880411938 1101 6 0.47727832777565027 1101 7 0.5076326503880964 1101 8 0.46122407620812567 1101 9 0.6372841332092847 1101 10-11 0.6504023492466899 1101 12-13 0.5816581068156594 1101 14-15 0.6876506678178629 1101 16-17 0.8777457993252611 1101 18-19 0.8559915544797931 1101 20-21 0.7469057067960634 1101 22-23 0.7756378762029073 1101 24-25 0.8243287576461569 1101 26-27 0.7874683932782531 1101 28-29 0.8067605208825981 1101 30-31 0.7832023703571238 1101 32-33 1.0608858560372383 1101 34-35 1.2345043057332035 1101 36-37 1.297604042307654 1101 38-39 1.2415738158549416 1101 40-41 1.4494577323397024 1101 42-43 1.446401947205672 1101 44-45 1.5803935375178568 1101 46-47 1.6361826606250105 1101 48-49 1.6096173645479155 1101 50-51 1.457252857335277 1101 52-53 1.5760523465749827 1101 54-55 1.565910447391282 1101 56-57 1.8537656911070926 1101 58-59 1.5769314189741692 1101 60-61 1.5320189417746697 1101 62-63 1.6719266123543726 1101 64-65 1.880910218238487 1101 66-67 1.6587809186464426 1101 68-69 1.7416906302882715 1101 70-71 1.7744659683556954 1101 72-73 1.7693791624802877 1101 74-75 1.9351133449950346 1101 76-77 1.8672524264713708 1101 78-79 2.0670956557739473 1101 80-81 2.3182929207608503 1101 82-83 2.024791389213938 1101 84-85 1.9073449024168099 1101 86-87 1.6553029038665308 1101 88-89 1.7232820136396363 1101 90-91 1.6188121913057785 1101 92-93 1.757721385459515 1101 94-95 1.7763573647817132 1101 96-97 1.698020645222158 1101 98-99 1.913331597376441 1101 100-101 1.9746866320985106 1102 1 0.48559061425408245 1102 2 0.4413673270604228 1102 3 0.46887556085318494 1102 4 0.4402731911556046 1102 5 0.5772717869503907 1102 6 0.5514211096909492 1102 7 0.45302947578491626 1102 8 0.4625887042078247 1102 9 0.5730127121606188 1102 10-11 0.6531564303420083 1102 12-13 0.7067502605807974 1102 14-15 0.7016655628754052 1102 16-17 0.846011051713738 1102 18-19 0.8141868452343246 1102 20-21 0.7721738930595095 1102 22-23 0.8310324447728306 1102 24-25 0.850817887263247 1102 26-27 0.7900629919392017 1102 28-29 0.7837416387641909 1102 30-31 0.7543255178783781 1102 32-33 0.9220308195518712 1102 34-35 1.0850376691575647 1102 36-37 1.1358783220037338 1102 38-39 0.9300263848762285 1102 40-41 1.0502521354089893 1102 42-43 1.1409464443094208 1102 44-45 1.3560498980083437 1102 46-47 1.4260555260429015 1102 48-49 1.7879774036662468 1102 50-51 1.6678163117849891 1102 52-53 1.8060756671196287 1102 54-55 1.9736122453300524 1102 56-57 1.926352014736068 1102 58-59 1.9291769617410353 1102 60-61 2.0268508081705114 1102 62-63 2.0558407024769885 1102 64-65 1.9823335237376263 1102 66-67 1.9613416649038697 1102 68-69 2.064993120320622 1102 70-71 1.8531953748102232 1102 72-73 1.8865814493982107 1102 74-75 2.081450514934101 1102 76-77 1.9228847141415706 1102 78-79 2.0019485858875434 1102 80-81 2.2733406902635913 1102 82-83 2.155849088755051 1102 84-85 1.8392150596424202 1102 86-87 1.8741752426914786 1102 88-89 1.9631428426473825 1102 90-91 2.048490217334514 1102 92-93 2.3437666724526665 1102 94-95 2.527570785379016 1102 96-97 2.532389109827591 1102 98-99 2.7216803523602664 1102 100-101 2.7996004965384387 1103 1 0.3795520491781623 1103 2 0.1729396207030156 1103 3 0.40757590894330775 1103 4 0.3559301270773858 1103 5 0.44986964761043424 1103 6 0.4858795303354455 1103 7 0.3369109211767167 1103 8 0.2965012256387425 1103 9 0.479424766969899 1103 10-11 0.366572331652975 1103 12-13 0.6635771757018389 1103 14-15 0.837241758821861 1103 16-17 0.7035370333990798 1103 18-19 0.5781739491591438 1103 20-21 0.6247171253139356 1103 22-23 0.800026639422363 1103 24-25 0.823928935516733 1103 26-27 0.6324723566987771 1103 28-29 0.773629418665017 1103 30-31 0.7866542196311457 1103 32-33 0.5799721426022089 1103 34-35 0.7078438258693112 1103 36-37 0.8499691410064969 1103 38-39 0.97420920050007 1103 40-41 1.155119816905568 1103 42-43 0.9919209619573337 1103 44-45 1.1572026939859654 1103 46-47 1.2941603494675036 1103 48-49 1.2200777013419923 1103 50-51 1.3371364681080493 1103 52-53 1.243737902323879 1103 54-55 1.5165683751990287 1103 56-57 1.3299956081652695 1103 58-59 1.6890072846432105 1103 60-61 1.650231342447487 1103 62-63 1.700759433343098 1103 64-65 1.6837043330423462 1103 66-67 1.461786158095041 1103 68-69 1.622300030361739 1103 70-71 1.5385225776010998 1103 72-73 1.7229959287158074 1103 74-75 1.3371851505747578 1103 76-77 1.4389172564309618 1103 78-79 1.825123999064168 1103 80-81 2.193400706465468 1103 82-83 1.839456045169701 1103 84-85 1.7364750766139778 1103 86-87 1.9062052103839413 1103 88-89 1.747457332163311 1103 90-91 2.088082341915758 1103 92-93 2.2409261492976498 1103 94-95 2.233546236270218 1103 96-97 2.3962953005979095 1103 98-99 3.049908748740439 1103 100-101 2.5876224633534655 1104 1 0.25458655149646603 1104 2 0.2078293778826641 1104 3 0.2502453374836229 1104 4 0.4025342126583311 1104 5 0.07878423633014364 1104 6 0.274350564847893 1104 7 0.2458222685777116 1104 8 0.4124315474155935 1104 9 0.16166917759433375 1104 10-11 0.24651692536127 1104 12-13 0.2964421215996751 1104 14-15 0.2778496580168479 1104 16-17 0.2525733741528384 1104 18-19 0.5385589570471936 1104 20-21 0.20105018094053406 1104 22-23 0.1595952401602716 1104 24-25 0.4057734990908983 1104 26-27 0.8174997433096038 1104 28-29 0.7610376551597327 1104 30-31 0.8454879526427064 1104 32-33 0.697677592828974 1104 34-35 0.687757778986672 1104 36-37 0.8790903637939778 1104 38-39 0.8415177157988367 1104 40-41 0.8448661077480821 1104 42-43 1.1617496025533285 1104 44-45 1.4562903134146339 1104 46-47 1.1518867163290665 1104 48-49 1.277265452196005 1104 50-51 1.2404730005554185 1104 52-53 1.5070228775455163 1104 54-55 1.3319461534269905 1104 56-57 1.5002838576252557 1104 58-59 1.4055919476346972 1104 60-61 1.201764511520242 1104 62-63 1.678527278955034 1104 64-65 1.5998693971976685 1104 66-67 1.3536375335030577 1104 68-69 1.6043233729210193 1104 70-71 1.1131552070449366 1104 72-73 1.5164213695224937 1104 74-75 1.7460053358870233 1104 76-77 1.6474530666720106 1104 78-79 1.7195393082176018 1104 80-81 1.8924505949185217 1104 82-83 1.7775848420073892 1104 84-85 1.6298344249063348 1104 86-87 1.5456477542113802 1104 88-89 1.4590382093958318 1104 90-91 1.427202779696369 1104 92-93 1.6014266291647594 1104 94-95 1.7011001295244732 1104 96-97 2.1599537871553025 1104 98-99 1.9421399661848113 1104 100-101 2.034076131488007 1105 1 0.11607140354735535 1105 2 0.22036223003815536 1105 3 -0.27791271897632086 1105 4 0.07701348147722342 1105 5 0.054424608196931956 1105 6 0.0937323559277985 1105 7 0.24548230597359577 1105 8 -0.03197149132707011 1105 9 0.024441964895423496 1105 10-11 0.06610465532635601 1105 12-13 0.0924547525179591 1105 14-15 0.23284383942612408 1105 16-17 -0.1343236825555394 1105 18-19 -0.1484498950559967 1105 20-21 0.1275496121569546 1105 22-23 0.021753479677002474 1105 24-25 0.06856655339338857 1105 26-27 -0.19777569177716003 1105 28-29 -0.13544373188769754 1105 30-31 0.02383429608339327 1105 32-33 0.13983164819057947 1105 34-35 0.052704365631377925 1105 36-37 0.5215902657278377 1105 38-39 0.39028600513317 1105 40-41 0.20732835228579205 1105 42-43 0.38900152791846665 1105 44-45 0.29420006641872476 1105 46-47 0.3597346222901834 1105 48-49 0.5159126625413251 1105 50-51 0.5804813688656694 1105 52-53 0.2320421638855592 1105 54-55 0.04602125901153187 1105 56-57 -0.6163690797823946 1105 58-59 -0.3775609440087564 1105 60-61 0.4902848896632612 1105 62-63 0.5073593766550601 1105 64-65 0.2947496911345695 1105 66-67 0.08589083179409229 1105 68-69 0.01637766233379523 1105 70-71 0.3502072082478769 1105 72-73 0.7625837147414423 1105 74-75 0.1751492838988966 1105 76-77 0.12547644563878535 1105 78-79 0.35722743175477945 1105 80-81 0.8601018455886411 1105 82-83 0.569475426350806 1105 84-85 -0.03566198587309799 1105 86-87 0.047429289011780895 1105 88-89 0.03297353804813952 1105 90-91 0.08873731858939493 1105 92-93 0.2906354315811086 1105 94-95 0.12106835609647604 1105 96-97 0.09379183420089277 1105 98-99 -0.03545845952682214 1105 100-101 0.173650393703781 1106 1 -0.4152435618482926 1106 2 -0.14393081488171333 1106 3 -0.42067212004471344 1106 4 -0.17399307098192196 1106 5 -0.5843217405833627 1106 6 -0.07761839799973558 1106 7 -0.5274447222625014 1106 8 0.10466964969553771 1106 9 -0.06289784195176651 1106 10-11 -0.08529267632280835 1106 12-13 -0.31504110285426634 1106 14-15 -0.04976714449534825 1106 16-17 -0.18055930793381236 1106 18-19 0.10583946575030012 1106 20-21 -0.3250634769722893 1106 22-23 -0.2793438598666995 1106 24-25 -0.41394048589505417 1106 26-27 -0.27016194477049993 1106 28-29 -0.20594187667335717 1106 30-31 -0.0679724984746386 1106 32-33 0.026768834054117008 1106 34-35 0.08184175675265948 1106 36-37 -0.08761080792811526 1106 38-39 0.026683731508789776 1106 40-41 0.06449658470073416 1106 42-43 0.29208302577377765 1106 44-45 0.1574141923627792 1106 46-47 0.7216724660311371 1106 48-49 0.09212118336972708 1106 50-51 0.06215290382528593 1106 52-53 -0.5061818302198589 1106 54-55 0.07257977062964471 1106 56-57 -0.2117613063027477 1106 58-59 0.1859571213053144 1106 60-61 -0.06543074939887106 1106 62-63 -0.014041375956711732 1106 64-65 0.5729989477330797 1106 66-67 0.3818040972344754 1106 68-69 0.09394141065621042 1106 70-71 0.6077793774013251 1106 72-73 0.26537969379462467 1106 74-75 0.4309451840067915 1106 76-77 0.287255083043064 1106 78-79 0.4772703253628414 1106 80-81 0.24585485208847047 1106 82-83 0.4314527427372141 1106 84-85 0.48766634759181215 1106 86-87 0.8679800639913893 1106 88-89 0.8185054977334119 1106 90-91 0.37341403762310676 1106 92-93 0.6350342631908035 1106 94-95 1.14682425725697 1106 96-97 0.5957917815706999 1106 98-99 0.7270806841799242 1106 100-101 0.5786528936379938 1107 1 -0.05714526800737474 1107 2 -0.059381635442338876 1107 3 0.021953673958797992 1107 4 -0.4352066768960299 1107 5 0.05491341453186038 1107 6 0.4756367454086856 1107 7 0.20087058113897882 1107 8 0.35762345115671934 1107 9 -0.06595461997765284 1107 10-11 0.20724748453742592 1107 12-13 -0.06750712058791208 1107 14-15 0.1681095871368825 1107 16-17 0.11996966641959972 1107 18-19 0.3979785599901149 1107 20-21 0.3782339410258544 1107 22-23 0.14385730214253556 1107 24-25 0.035124053311918146 1107 26-27 0.40496325305290526 1107 28-29 0.1379456512387094 1107 30-31 0.42127458024280884 1107 32-33 0.7616095997661674 1107 34-35 0.7122426065803111 1107 36-37 0.6304718768335249 1107 38-39 0.41093807278396355 1107 40-41 1.0464377471435569 1107 42-43 0.48164662193221375 1107 44-45 0.12816884798746742 1107 46-47 0.9230187491191373 1107 48-49 0.6197514216249758 1107 50-51 0.9852781883657897 1107 52-53 1.1239263493712741 1107 54-55 1.5168965482219434 1107 56-57 1.128115555405138 1107 58-59 1.0060950015056278 1107 60-61 0.8854114905040014 1107 62-63 0.9034472966211702 1107 64-65 0.8043710291604427 1107 66-67 0.711188188981172 1107 68-69 1.1721602412760213 1107 70-71 0.8375797112621832 1107 72-73 0.5925850182250016 1107 74-75 0.8445532660411708 1107 76-77 1.1590330005317355 1107 78-79 1.7442072603466912 1107 80-81 2.1697280716778664 1107 82-83 2.120539394806503 1107 84-85 1.9702036901460644 1107 86-87 1.6968655323721649 1107 88-89 1.0509290240845708 1107 90-91 1.1123955452000125 1107 92-93 1.351100775211961 1107 94-95 1.7756533595181203 1107 96-97 1.1404402520977257 1107 98-99 1.9647289162349004 1107 100-101 1.5653634969722674 1108 1 -0.14766277846370812 1108 2 -0.1948457717804395 1108 3 -0.2940148634030848 1108 4 -0.19929931688604086 1108 5 -0.06753492806131334 1108 6 -0.5287954913102126 1108 7 -0.30399863666487903 1108 8 -0.24762656444974596 1108 9 -0.5794073948075393 1108 10-11 -0.5367596320116732 1108 12-13 -0.37128700697282824 1108 14-15 -0.39737514973807464 1108 16-17 -0.527121288071946 1108 18-19 -0.5077739838640625 1108 20-21 -0.12813349764073223 1108 22-23 -0.4057868704025651 1108 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-1.3118867266684866 2316 60-61 -0.7413727547368048 2316 62-63 -0.9053480040845905 2316 64-65 -0.9869245962815576 2316 66-67 -0.9494624381205945 2316 68-69 -1.1282063498594006 2316 70-71 -0.8659505325064849 2316 72-73 -1.0868714454694874 2316 74-75 -1.059981495169442 2316 76-77 -1.054780557567188 2316 78-79 -0.7532164988000467 2316 80-81 -1.0424401561282188 2316 82-83 -0.8677628841202818 2316 84-85 -0.7900110687553159 2316 86-87 -0.4751219977561405 2316 88-89 -0.4827497323921115 2316 90-91 -0.6143425153308222 2316 92-93 -1.0449403291799158 2316 94-95 -1.5599507220556248 2316 96-97 -1.9237709274886328 2316 98-99 -1.6608285476472133 2316 100-101 -2.2142632728402525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 18.0 6 43.0 7 115.0 8 262.0 9 423.0 10 799.0 11 1167.0 12 1587.0 13 2256.0 14 3089.0 15 3914.0 16 5120.0 17 6702.0 18 5825.0 19 4005.0 20 3779.0 21 3896.0 22 4152.0 23 4516.0 24 5243.0 25 6056.0 26 7358.0 27 8680.0 28 10576.0 29 12850.0 30 15198.0 31 18631.0 32 22426.0 33 27679.0 34 35500.0 35 46026.0 36 60855.0 37 70135.0 38 33398.0 39 1310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.07843137254902 21.894579008073816 16.25997693194925 20.767012687427915 2 15.506572164651233 22.808721062986223 40.02412522977782 21.66058154258472 3 18.60022603039878 27.669580459902672 31.64702354867726 22.083169961021287 4 12.676257731816106 19.50032057343438 38.48172731424037 29.341694380509136 5 12.296494129205904 39.43014896664337 34.463210382230095 13.810146521920629 6 28.669873938532923 39.476427474296464 17.76668711571548 14.087011471455128 7 25.78217611704851 35.62354992412327 21.791613429951244 16.802660528876977 8 25.64501138153631 35.88747154615922 20.17218753819791 18.295329534106557 9 26.40902402686359 17.176970373479584 21.22449826798094 35.189507331675884 10-11 23.624993657719823 28.627553632627457 27.79106453443051 19.956388175222212 12-13 25.07576141956374 26.495394351449956 28.586732411127358 19.84211181785894 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28.04951882326485 32.00583524093433 19.70154933719274 40-41 20.311761219090847 28.049168644437216 31.859634798613367 19.779435337858565 42-43 20.239435190353397 28.226989450182444 31.47865818498854 20.054917174475623 44-45 20.34894917404215 28.328422752039668 31.242367457548937 20.080260616369245 46-47 20.75913166315911 28.540605990716983 30.58275434600859 20.11750800011532 48-49 20.62291853095565 28.827827338394975 30.24057400112549 20.30868012952388 50-51 20.67526848489531 29.057140320186903 29.802544105555818 20.465047089361967 52-53 20.733015205994757 29.052721649341873 29.86664483289089 20.347618311772482 54-55 20.60479351270567 29.03864515920717 29.678541281314434 20.678020046772726 56-57 20.756307463096192 29.369674591524088 29.437018774497307 20.436999170882405 58-59 20.800915147091214 28.981656697605928 29.54854972941121 20.668878425891652 60-61 20.706761455036066 28.914834610439407 29.81301783335928 20.565386101165252 62-63 20.58819459499352 28.666760455537155 30.153160300554894 20.591884648914434 64-65 20.379322911140736 28.728417158330295 29.90748727978928 20.984772650739686 66-67 20.49767869868748 28.93589149271321 29.492634798071006 21.073795010528308 68-69 20.329226174345727 28.779787473261187 29.558168095619735 21.332818256773354 70-71 20.402190158126835 28.83093160138015 29.578666715869883 21.188211524623135 72-73 20.475920823640806 28.64047829662187 29.522752881232 21.36084799850532 74-75 20.29750983415424 28.854579065908148 29.178174923973426 21.669736175964186 76-77 20.195671878279363 28.712610964913292 29.396678375305306 21.695038781502042 78-79 20.168054628133582 28.594312760134656 29.300575828134967 21.9370567835968 80-81 20.17451709135106 28.86808357539786 29.027333015833847 21.93006631741723 82-83 19.93777266264042 28.78983051238377 29.20983044319103 22.06256638178478 84-85 19.82317539354257 28.642265163107844 29.067778921962624 22.466780521386966 86-87 19.77303862102685 28.673794620823895 29.172528471072283 22.38063828707697 88-89 19.770729693514426 28.46528887689608 28.93831311852359 22.825668311065908 90-91 19.579376098377747 28.934902235732046 28.79398700166515 22.69173466422506 92-93 19.562219208664285 28.94514709545291 28.526324043579738 22.966309652303067 94-95 19.386963194959357 29.00618202910316 28.291460589420325 23.315394186517157 96-97 19.845201523999297 28.969824444874952 27.73849389754518 23.446480133580568 98-99 19.612794903994402 29.18787023359383 27.56331088148028 23.63602398093149 100-101 19.64392793224967 28.951871805752873 27.496010073986604 23.90819018801085 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 530.0 1 359.5 2 193.5 3 210.5 4 218.5 5 231.0 6 345.5 7 676.5 8 1054.5 9 1215.0 10 1105.0 11 951.5 12 930.5 13 966.0 14 1041.0 15 1085.0 16 1091.5 17 1140.0 18 1214.0 19 1261.0 20 1391.5 21 1606.5 22 1810.0 23 2088.5 24 2457.5 25 3017.0 26 3829.5 27 4583.5 28 5176.0 29 5903.0 30 6780.0 31 7757.5 32 8851.0 33 10014.0 34 11397.0 35 12483.0 36 13650.0 37 14773.0 38 15261.0 39 15292.0 40 15277.5 41 15514.5 42 15412.0 43 15602.0 44 16168.5 45 16317.0 46 15868.0 47 15468.5 48 15658.0 49 15057.5 50 14014.5 51 13188.0 52 12158.5 53 11562.0 54 10766.0 55 9346.0 56 8022.5 57 6808.0 58 5982.0 59 5326.0 60 4352.5 61 3350.0 62 2513.0 63 1929.5 64 1387.5 65 943.5 66 638.5 67 454.0 68 323.5 69 189.5 70 110.0 71 72.0 72 49.5 73 35.0 74 17.0 75 12.0 76 12.0 77 4.5 78 1.5 79 3.0 80 3.0 81 2.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022601580265591628 2 0.006226965991540552 3 0.006457594361597608 4 0.0 5 0.002536912070627632 6 0.0 7 0.0 8 2.3062837005705745E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.1531418502852873E-4 18-19 0.0046125674011411495 20-21 6.918851101711724E-4 22-23 0.0 24-25 0.004958509956226736 26-27 0.0047278815861696785 28-29 0.00541976669634085 30-31 0.023293465375762806 32-33 2.3062837005705745E-4 34-35 0.002652226255656161 36-37 0.01372238801839492 38-39 0.005881023436454965 40-41 0.006688222731654666 42-43 0.008302621322054069 44-45 0.0024215978855991033 46-47 0.0024215978855991033 48-49 0.0023062837005705748 50-51 0.001729712775427931 52-53 0.0043819390310840915 54-55 0.0027675404406846895 56-57 0.0010378276652567586 58-59 0.0014990844053708735 60-61 1.1531418502852873E-4 62-63 0.0 64-65 0.008302621322054069 66-67 0.0011531418502852874 68-69 0.001268456035313816 70-71 0.005073824141255264 72-73 0.01372238801839492 74-75 0.007495422026854368 76-77 0.003459425550855862 78-79 0.011185475947767287 80-81 3.459425550855862E-4 82-83 0.006111651806512022 84-85 0.0010378276652567586 86-87 0.0 88-89 0.0011531418502852874 90-91 9.225134802282298E-4 92-93 4.612567401141149E-4 94-95 5.765709251426437E-4 96-97 4.612567401141149E-4 98-99 8.07199295199701E-4 100-101 0.0013837702203423447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 433598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.41797639394778 #Duplication Level Percentage of deduplicated Percentage of total 1 86.33717489581008 59.07014793941674 2 8.263438425460269 11.307354702519634 3 2.1595549298077583 4.432571346270622 4 0.898212842423478 2.45815620198681 5 0.5402197630222255 1.848037149699934 6 0.3315228500470671 1.3609273517144715 7 0.25391324757951106 1.2160561405303922 8 0.18612766701324415 1.0187582662378167 9 0.138100701438643 0.8503713477915035 >10 0.8275418697912198 10.318257433623666 >50 0.0452728222492384 2.0854789838455363 >100 0.01689284406900319 1.710083516292061 >500 0.0010135706441401912 0.4465911534842203 >1k 0.0010135706441401912 1.8772084665866218 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.803047984538674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2733 0.6303073353659381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 0.43957767332875153 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 759 0.1750469328733066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.15083095401731558 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 519 0.11969612405961282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 472 0.10885659066693111 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1531418502852873E-4 0.0 12-13 0.0 0.0 0.0 2.3062837005705745E-4 0.0 14-15 0.0 0.0 0.0 2.3062837005705745E-4 0.0 16-17 0.0 0.0 0.0 2.3062837005705745E-4 0.0 18-19 0.0 0.0 0.0 4.612567401141149E-4 0.0 20-21 0.0 0.0 0.0 6.918851101711724E-4 0.0 22-23 0.0 0.0 0.0 0.002075655330513517 0.0 24-25 0.0 0.0 0.0 0.003344111365827333 0.0 26-27 0.0 0.0 0.0 0.0046125674011411495 0.0 28-29 0.0 0.0 0.0 0.00818730713702554 0.0 30-31 0.0 0.0 0.0 0.019718725639878414 0.0 32-33 0.0 0.0 0.0 0.03955276546478535 0.0 34-35 0.0 0.0 0.0 0.07276325075300163 0.0 36-37 0.0 0.0 0.0 0.12315554961046868 0.0 38-39 0.0 0.0 0.0 0.20422142168552437 0.0 40-41 0.0 0.0 0.0 0.311002357021942 0.0 42-43 0.0 0.0 0.0 0.42043551861401574 0.0 44-45 0.0 0.0 0.0 0.5540846590620805 0.0 46-47 0.0 0.0 0.0 0.7238271394240748 0.0 48-49 0.0 0.0 0.0 0.9055622950290361 0.0 50-51 0.0 0.0 0.0 1.1069008620888474 0.0 52-53 0.0 0.0 0.0 1.3006286929367756 0.0 54-55 0.0 0.0 0.0 1.509693310393498 0.0 56-57 0.0 0.0 0.0 1.781604158690769 0.0 58-59 0.0 0.0 0.0 2.0691977361519194 0.0 60-61 0.0 0.0 0.0 2.390347741456372 0.0 62-63 0.0 0.0 0.0 2.74712982993464 0.0 64-65 0.0 0.0 0.0 3.106679458853592 0.0 66-67 0.0 0.0 0.0 3.4919441510339073 0.0 68-69 0.0 0.0 0.0 3.9723430458627575 0.0 70-71 0.0 0.0 0.0 4.560676017878311 0.0 72-73 0.0 0.0 0.0 5.149585560819007 0.0 74-75 0.0 0.0 0.0 5.698365767369776 0.0 76-77 0.0 0.0 0.0 6.3827554555140935 0.0 78-79 0.0 0.0 0.0 7.094359291325144 0.0 80-81 0.0 0.0 0.0 7.82129991374499 0.0 82-83 0.0 0.0 0.0 8.527022726119586 0.0 84-85 0.0 0.0 0.0 9.268492935853025 0.0 86-87 0.0 0.0 0.0 9.94446468849026 0.0 88-89 0.0 0.0 0.0 10.629200319189664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1730 0.0 49.429104 1 GTATCAA 2875 0.0 39.988388 1 TAGGGCG 30 0.0039498582 31.66759 30-31 ATCAACG 3670 0.0 31.18941 3 TCAACGC 3710 0.0 30.725115 4 TATCAAC 3740 0.0 30.478657 2 CAACGCA 3785 0.0 30.116293 5 AACGCAG 3905 0.0 29.190825 6 AGCCGTA 70 0.008389433 27.140516 7 CCATACG 45 8.944245E-4 26.389658 62-63 ACAGCCG 90 8.943024E-4 26.386614 5 TACACTC 90 8.943024E-4 26.386614 5 GGTAAGG 145 7.8586345E-7 26.210684 1 CGCAGAG 4345 0.0 26.016169 8 ACGCAGA 4350 0.0 25.87708 7 GTTATAT 155 1.4054822E-6 24.519672 1 GCAGAGT 4495 0.0 24.302687 9 GAGTACT 2590 0.0 23.839643 12-13 CAGCCGT 100 0.0016528196 23.747953 6 TAAGGGT 145 2.3315606E-5 22.929058 3 >>END_MODULE