FastQCFastQC Report
Thu 26 May 2016
SRR936785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463533
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49681.0717683530622415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33640.7257304226452054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.523587317407822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.16115357482638776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.14152174710322674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.13332384102102765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.053.967251
GTATCAA31350.040.782921
ATCAACG38700.032.914513
TCAACGC38900.032.745284
CAACGCA39650.032.0060165
TATCAAC39750.031.9254972
TACACGG753.0644386E-431.6863565
AACGCAG40800.031.220386
GATTGGC1802.0918378E-1029.0364199
ACGCAGA46100.027.212777
GGATATG2104.1836756E-1127.1597351
GGCTCGT700.00836109727.1597356
AGGCTCG700.00836109727.1597355
CGCAGAG46050.027.0359368
GCAGAGT46900.026.1378429
GAGTACT28550.026.04399912-13
TAGGCTC1109.424744E-525.92524
GTACATG25800.025.4227731
TACATGG25850.025.1897342
ACATGGG25550.024.369353
Produced by FastQC (version 0.11.5)