FastQCFastQC Report
Thu 26 May 2016
SRR936785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463533
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.7591692500857544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.5747163632362744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.424349506939096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.1669784028321608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.15273993437360447No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.11628082574487685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.11045599773910379No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.044.5377771
GTATCAA31200.037.907361
TATCAAC39750.029.5115342
ATCAACG40350.029.0727023
TCAACGC40800.028.7520454
CAACGCA41900.028.0032585
AACGCAG43050.027.2552076
ACGCAGA47400.024.7539377
CGCAGAG47950.024.1727968
TAATACG1000.001653528123.7466283
GAGTACT27700.023.660912-13
GTGCATA2052.6615453E-823.1699431
ACTTACC1252.2519568E-422.8016838
GCAGAGT49250.022.6666479
CTAGAGT1703.1461423E-622.3497683
GTACTTT29800.021.91383614-15
CTAAGCC1100.002877173221.5878433
AGTACTT29750.021.47174312-13
GTACATG24350.021.0670431
TATTGGG1604.9864997E-520.77832
Produced by FastQC (version 0.11.5)