##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936785_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 463533 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.628145137455153 31.0 31.0 34.0 30.0 34.0 2 31.7752867649121 31.0 31.0 34.0 30.0 34.0 3 31.8547223175049 31.0 31.0 34.0 30.0 34.0 4 35.651204984326895 37.0 35.0 37.0 33.0 37.0 5 35.52493565722397 37.0 35.0 37.0 33.0 37.0 6 35.5645703757877 37.0 35.0 37.0 33.0 37.0 7 35.493526890210624 37.0 35.0 37.0 33.0 37.0 8 35.45591576004298 37.0 35.0 37.0 33.0 37.0 9 37.00848267545137 39.0 37.0 39.0 33.0 39.0 10-11 36.90731296369407 39.0 37.0 39.0 33.0 39.0 12-13 36.781118064948984 39.0 37.0 39.0 32.0 39.0 14-15 37.776372987467994 39.0 37.0 41.0 32.5 41.0 16-17 37.67710497418739 39.0 37.0 41.0 32.0 41.0 18-19 37.87561295528042 39.5 37.0 41.0 33.0 41.0 20-21 37.92666433673547 40.0 37.0 41.0 33.0 41.0 22-23 37.815136139174555 40.0 37.0 41.0 32.5 41.0 24-25 37.6703158135451 39.0 37.0 41.0 32.0 41.0 26-27 37.42039833194185 39.0 36.0 41.0 32.0 41.0 28-29 37.27361913822749 39.0 36.0 41.0 32.0 41.0 30-31 37.2192832009803 39.0 36.0 40.0 31.5 41.0 32-33 36.98580683575926 39.0 35.5 40.0 31.0 41.0 34-35 36.76953420792047 39.0 35.0 40.0 30.5 41.0 36-37 36.55081730966296 38.5 35.0 40.0 30.0 41.0 38-39 36.33107783048888 38.0 35.0 40.0 30.0 41.0 40-41 36.11227679582683 38.0 35.0 40.0 30.0 41.0 42-43 35.775514364673064 38.0 35.0 40.0 29.0 41.0 44-45 35.401162376788704 38.0 34.5 40.0 27.5 41.0 46-47 34.90473709530929 38.0 34.0 40.0 25.5 41.0 48-49 34.428269400452606 38.0 33.5 40.0 24.0 41.0 50-51 34.055683198391485 38.0 33.0 40.0 23.0 41.0 52-53 33.74115758748568 38.0 33.0 40.0 22.5 41.0 54-55 33.36979999266504 37.0 33.0 40.0 19.5 41.0 56-57 33.02866138980396 37.0 32.0 40.0 18.5 41.0 58-59 32.638489600524665 36.5 31.0 39.0 16.0 40.0 60-61 32.249263806460384 36.0 31.0 39.0 13.5 40.0 62-63 31.833449829893446 36.0 30.5 39.0 12.0 40.0 64-65 31.392422977436343 35.0 30.0 38.5 8.0 40.0 66-67 30.927231718130102 35.0 29.0 38.0 7.5 40.0 68-69 30.560763742818743 34.5 29.0 38.0 7.0 40.0 70-71 30.04571734914235 34.0 28.5 37.0 2.0 39.0 72-73 29.43483527602134 34.0 28.0 36.5 2.0 39.0 74-75 28.849474578940445 33.5 26.5 36.0 2.0 39.0 76-77 27.61995262473222 31.5 26.0 34.5 2.0 37.0 78-79 28.3092972884347 33.0 26.0 35.0 2.0 37.0 80-81 27.935061797110457 33.0 26.0 35.0 2.0 37.0 82-83 27.5657159252955 33.0 26.0 35.0 2.0 36.5 84-85 27.157008238895614 32.5 26.0 35.0 2.0 36.0 86-87 26.718692088804897 32.0 25.0 35.0 2.0 36.0 88-89 26.188374937706698 32.0 24.5 34.0 2.0 35.0 90-91 25.578803450886994 31.5 22.0 34.0 2.0 35.0 92-93 24.884362062679465 31.0 19.0 34.0 2.0 35.0 94-95 24.011729477728665 31.0 12.0 34.0 2.0 35.0 96-97 22.96998487702062 30.0 2.0 34.0 2.0 35.0 98-99 21.78753724114572 30.0 2.0 34.0 2.0 35.0 100-101 18.36501284698177 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4219531162324479 1101 2 0.5042559707424523 1101 3 0.47784336678574846 1101 4 0.4944947800581403 1101 5 0.5355305499021981 1101 6 0.5329431431966896 1101 7 0.5701080759230166 1101 8 0.564080951034974 1101 9 0.6598059902218765 1101 10-11 0.645547522548199 1101 12-13 0.7055649775961683 1101 14-15 0.8404453980878159 1101 16-17 0.7882367786995772 1101 18-19 0.7456590210917398 1101 20-21 0.8332088815749117 1101 22-23 0.7315566994832423 1101 24-25 0.8714345554912413 1101 26-27 0.76067934980545 1101 28-29 0.8916777876699982 1101 30-31 1.1079212435509191 1101 32-33 1.0666636316178497 1101 34-35 1.1787737071155178 1101 36-37 1.14697590954124 1101 38-39 1.38293141769757 1101 40-41 1.2815801649249892 1101 42-43 1.3410462156106107 1101 44-45 1.4792509882706923 1101 46-47 1.4664338392631464 1101 48-49 1.624883788386171 1101 50-51 1.6815573023710968 1101 52-53 1.737040190872996 1101 54-55 1.7989044761164337 1101 56-57 1.823146254195123 1101 58-59 1.742631023746977 1101 60-61 1.9360235004630013 1101 62-63 1.9808001823504036 1101 64-65 1.9960001871370814 1101 66-67 1.6055257246127965 1101 68-69 1.60564341776686 1101 70-71 1.4632017213040065 1101 72-73 1.715283664701417 1101 74-75 1.7717737705768393 1101 76-77 1.8087193608430248 1101 78-79 1.9681106654150256 1101 80-81 2.242869747909925 1101 82-83 2.0938957106131575 1101 84-85 2.0723519278658067 1101 86-87 1.7457679159519053 1101 88-89 1.898352029380117 1101 90-91 1.84158141602693 1101 92-93 1.9482881776686476 1101 94-95 1.9755627895356902 1101 96-97 1.9918162783404725 1101 98-99 2.034956273881189 1101 100-101 1.702706026510075 1102 1 0.5096632691305203 1102 2 0.5554734553178875 1102 3 0.502410677629932 1102 4 0.48369621725629486 1102 5 0.5340231490237102 1102 6 0.4831315341612097 1102 7 0.4230165264733472 1102 8 0.5488507844423651 1102 9 0.5499505659461192 1102 10-11 0.530445332514617 1102 12-13 0.5133420006365057 1102 14-15 0.7791946605494786 1102 16-17 0.7341005544587915 1102 18-19 0.6210997230097064 1102 20-21 0.7507691685046396 1102 22-23 0.6309259676266805 1102 24-25 0.7266842349807519 1102 26-27 0.6735961044910965 1102 28-29 0.8010233999542891 1102 30-31 1.0650534213468248 1102 32-33 0.8493761883445643 1102 34-35 0.8829655198936877 1102 36-37 0.9130228315479272 1102 38-39 1.1378665507458336 1102 40-41 1.1785581243658427 1102 42-43 1.161046343086376 1102 44-45 1.1187631931642983 1102 46-47 1.4824504857765035 1102 48-49 1.6904117929898632 1102 50-51 1.7448417335742405 1102 52-53 1.4739411458485492 1102 54-55 1.4360306079190792 1102 56-57 1.653660400362476 1102 58-59 1.6980463761992404 1102 60-61 1.8088659732013923 1102 62-63 2.004508397616899 1102 64-65 1.9293253559514163 1102 66-67 1.7789860905774937 1102 68-69 1.7576530276181863 1102 70-71 1.5369689851642647 1102 72-73 1.654248725416302 1102 74-75 1.725926657720727 1102 76-77 1.609463136383706 1102 78-79 1.4262478530083555 1102 80-81 1.7844052207721575 1102 82-83 1.7208228709630973 1102 84-85 1.723928575398972 1102 86-87 1.399018138123946 1102 88-89 1.4409129992157084 1102 90-91 1.6663113495210062 1102 92-93 1.6427324197706135 1102 94-95 1.577870510560448 1102 96-97 2.1906748239513014 1102 98-99 2.2165454959876705 1102 100-101 2.2561242890518685 1103 1 0.36653502254231185 1103 2 0.47182988572087936 1103 3 0.30373924728341706 1103 4 0.35681482997977554 1103 5 0.32718633249155005 1103 6 0.29889799120660143 1103 7 0.28235483972402164 1103 8 0.23430187361774557 1103 9 0.5592842801156479 1103 10-11 0.4689140644107752 1103 12-13 0.5196734023557568 1103 14-15 0.6854936266437548 1103 16-17 0.49242448791573423 1103 18-19 0.6437477771142639 1103 20-21 0.6486506058062602 1103 22-23 0.6037256300379923 1103 24-25 0.645360780796473 1103 26-27 0.6147770839824247 1103 28-29 0.6764125064254358 1103 30-31 0.8982274005525284 1103 32-33 0.8547349247429779 1103 34-35 0.8184315359114009 1103 36-37 0.7301000267216367 1103 38-39 0.6821080977153642 1103 40-41 0.8291998172149988 1103 42-43 1.2084188960928017 1103 44-45 1.3008270994140503 1103 46-47 1.3966312280520654 1103 48-49 1.444275828013886 1103 50-51 1.7070937664488 1103 52-53 1.565371686006607 1103 54-55 1.4214603960870207 1103 56-57 1.4427513798699394 1103 58-59 1.6119180924956389 1103 60-61 1.4766465050488264 1103 62-63 1.5890399126187837 1103 64-65 1.8182765072547262 1103 66-67 1.7893130449045813 1103 68-69 1.5423760903157628 1103 70-71 1.2858994970823403 1103 72-73 1.3673136660558818 1103 74-75 1.862253105342031 1103 76-77 1.3548176609920155 1103 78-79 1.6923329376233838 1103 80-81 1.9396593079029607 1103 82-83 1.9942785882664538 1103 84-85 1.9139541366079023 1103 86-87 1.8226790389951901 1103 88-89 1.5467685170641765 1103 90-91 1.6168034512042695 1103 92-93 1.6212530970780996 1103 94-95 1.2072515387858935 1103 96-97 1.4049565039553045 1103 98-99 1.1910774789662248 1103 100-101 1.2033070694465486 1104 1 0.17973305129453365 1104 2 0.4217224779894657 1104 3 0.36252189485969666 1104 4 0.21876916468364982 1104 5 0.2547371446106368 1104 6 0.09186351381082147 1104 7 0.12033440795466532 1104 8 0.2690951744857557 1104 9 0.38717918578152677 1104 10-11 0.4771737675571899 1104 12-13 0.20988228852299784 1104 14-15 0.2948599696292078 1104 16-17 0.5000985516216403 1104 18-19 0.4480397123002575 1104 20-21 0.2991745222517892 1104 22-23 0.3560438874083047 1104 24-25 0.2619564903193137 1104 26-27 0.051863369813354154 1104 28-29 0.5150287449192064 1104 30-31 0.6617189780367525 1104 32-33 0.3757960742487114 1104 34-35 0.30298620693270095 1104 36-37 0.21918186072080204 1104 38-39 0.5100759045564089 1104 40-41 0.6331539769560521 1104 42-43 0.6412113027063882 1104 44-45 0.6040279470117085 1104 46-47 0.6242359870407839 1104 48-49 0.6604351025013955 1104 50-51 0.9758949795243552 1104 52-53 0.7485140182609982 1104 54-55 0.796046074408153 1104 56-57 1.3857815415161454 1104 58-59 1.0448903220079835 1104 60-61 1.2141075029201147 1104 62-63 1.4187937982514107 1104 64-65 1.379792948899766 1104 66-67 1.2276289305566586 1104 68-69 1.512955606057556 1104 70-71 1.2323608740397347 1104 72-73 1.4765536470043727 1104 74-75 1.5595820062839145 1104 76-77 1.6318810072531598 1104 78-79 1.743631064549188 1104 80-81 1.9878737151192034 1104 82-83 1.876630671782042 1104 84-85 1.89626588147323 1104 86-87 1.862235216640773 1104 88-89 1.4111723312536633 1104 90-91 1.4838122673863872 1104 92-93 1.2799249348681236 1104 94-95 1.2419476380870158 1104 96-97 1.18415828468801 1104 98-99 1.1279717917025494 1104 100-101 1.4045556235732413 1105 1 0.055249833580287344 1105 2 0.2884919171784972 1105 3 0.045048230318553095 1105 4 -0.030302379006350577 1105 5 -0.13370795384076928 1105 6 0.002182809351893411 1105 7 -0.04227975129602868 1105 8 0.15786727641741294 1105 9 0.10307155492174047 1105 10-11 0.23148320627907992 1105 12-13 0.22192302381368023 1105 14-15 0.4288730030536385 1105 16-17 0.2338184497834206 1105 18-19 0.1845916941749195 1105 20-21 0.24701746715170003 1105 22-23 0.3830742286597868 1105 24-25 0.2434340789022187 1105 26-27 0.3690801030485815 1105 28-29 0.4238940428427327 1105 30-31 0.864799817968553 1105 32-33 0.7158563363186445 1105 34-35 0.36796646993692406 1105 36-37 0.2478297013299553 1105 38-39 0.6805089505938255 1105 40-41 0.6336094460892525 1105 42-43 0.5507831617211778 1105 44-45 0.6455398709600502 1105 46-47 0.6403350690952934 1105 48-49 1.0698668171674939 1105 50-51 1.2538012229550901 1105 52-53 1.0456025194171943 1105 54-55 1.274037572317667 1105 56-57 1.2162827911365852 1105 58-59 1.2357786036046967 1105 60-61 1.2818672955064727 1105 62-63 1.6004734282703375 1105 64-65 0.7164255137749187 1105 66-67 1.2138363909074883 1105 68-69 0.938632386474719 1105 70-71 0.9261454721758149 1105 72-73 1.4907724590474452 1105 74-75 1.2005847390987157 1105 76-77 1.2063210137932252 1105 78-79 1.255835301579996 1105 80-81 1.3676590606918104 1105 82-83 1.4988365438302473 1105 84-85 1.2033688642121163 1105 86-87 1.514414461262735 1105 88-89 1.128448625837084 1105 90-91 0.903375250592422 1105 92-93 1.1728429737815382 1105 94-95 0.7514482570578913 1105 96-97 0.8488395730817189 1105 98-99 1.0572280923588941 1105 100-101 1.1645876231995267 1106 1 -0.10840000234646041 1106 2 -0.10316681045990705 1106 3 5.367451909989995E-4 1106 4 -0.12789949978542836 1106 5 -0.11026135703755102 1106 6 -0.15273409548562 1106 7 -0.12987369646381097 1106 8 -0.22362949504442753 1106 9 0.12381888211556458 1106 10-11 -0.1916405367081211 1106 12-13 0.18225434592651624 1106 14-15 0.15386506402537492 1106 16-17 -0.31172510901831885 1106 18-19 -0.3401516105779123 1106 20-21 0.0262330911593196 1106 22-23 -0.18049092429882307 1106 24-25 -0.16301770473280186 1106 26-27 0.02394408103101142 1106 28-29 -0.18881369506348022 1106 30-31 -0.23663449980428908 1106 32-33 -0.24574523031626683 1106 34-35 -0.34808095252523685 1106 36-37 -0.8383881456055704 1106 38-39 -0.21657477969091588 1106 40-41 0.06435465620887726 1106 42-43 -0.27053577550757524 1106 44-45 -0.2542695923616449 1106 46-47 -0.048349169287263294 1106 48-49 -0.18158867134737022 1106 50-51 -0.223360839075184 1106 52-53 -0.14128220589243057 1106 54-55 -0.43534106640362324 1106 56-57 -0.5456522146853651 1106 58-59 -0.3690959597487513 1106 60-61 -0.6974647675823356 1106 62-63 0.04897559827139375 1106 64-65 -0.37685806831463253 1106 66-67 -0.8341947300833041 1106 68-69 -0.4998136810599476 1106 70-71 -0.7342758255906716 1106 72-73 -0.4839814310764048 1106 74-75 -0.5269478109171608 1106 76-77 -0.7237361472102641 1106 78-79 -0.22881591044498606 1106 80-81 -0.3813617924890913 1106 82-83 -0.09762264956447808 1106 84-85 -0.2967634529255996 1106 86-87 -0.38756500311749065 1106 88-89 -0.5477978016002005 1106 90-91 -0.7816041079340899 1106 92-93 -0.6708375597211607 1106 94-95 -0.8676900937413059 1106 96-97 -0.7465346197837377 1106 98-99 -0.8181291639129391 1106 100-101 -0.8258326160298601 1107 1 -0.12267305199184975 1107 2 -0.17444340620458476 1107 3 -0.27118311296502995 1107 4 -0.16344914517550535 1107 5 -0.3263074563283297 1107 6 -0.5379291309466083 1107 7 -0.3989339801517531 1107 8 -0.5039486439805998 1107 9 -0.5790179973170595 1107 10-11 -0.3137593310343618 1107 12-13 0.0672277501818428 1107 14-15 0.004308326436721188 1107 16-17 -0.06123752036583596 1107 18-19 -0.2280948729892529 1107 20-21 0.026277417400457637 1107 22-23 -0.16754766188747539 1107 24-25 -0.24098756288882583 1107 26-27 -0.3344335785434609 1107 28-29 -0.42374277307766306 1107 30-31 -0.271829535265276 1107 32-33 -0.4389633154226473 1107 34-35 -0.9534887539436738 1107 36-37 -0.7766416917048673 1107 38-39 -1.1009276165703525 1107 40-41 -1.4515584643584987 1107 42-43 -0.9732840024579232 1107 44-45 -1.2753245569006566 1107 46-47 -0.8954236373723674 1107 48-49 -1.4454627848225527 1107 50-51 -1.5352845979404321 1107 52-53 -1.4527183761051994 1107 54-55 -1.3026726266873112 1107 56-57 -1.0473366118484861 1107 58-59 -1.0781828391813768 1107 60-61 -1.1708690229014849 1107 62-63 -0.9743400045654873 1107 64-65 -1.1289414016479675 1107 66-67 -0.787873808097487 1107 68-69 -1.195602688152146 1107 70-71 -0.832281144739607 1107 72-73 -1.2810558991615117 1107 74-75 -0.9688804350306377 1107 76-77 -0.9476279911818963 1107 78-79 -1.0427343501612967 1107 80-81 -0.7533919343330666 1107 82-83 -0.8494843516921371 1107 84-85 -0.6942368571809183 1107 86-87 -0.24301713077706566 1107 88-89 -0.46362226968530607 1107 90-91 -0.07025659020359498 1107 92-93 -0.4861744391537819 1107 94-95 -0.035243285230667 1107 96-97 0.16255225964888353 1107 98-99 -0.0023490220689659225 1107 100-101 0.41082518538857826 1108 1 -0.10389164495667558 1108 2 -0.34219885009068207 1108 3 -0.07970488850941848 1108 4 -0.46627577834133405 1108 5 -0.2503233692261517 1108 6 0.02289582717734362 1108 7 -0.06570533693567171 1108 8 -0.8491328985869657 1108 9 -0.8366394378530728 1108 10-11 -0.45535271461894666 1108 12-13 -0.1710993017444551 1108 14-15 -0.053501556310344256 1108 16-17 -0.23739958066734346 1108 18-19 -0.27139470548507205 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-1.4655688973112078 2316 48-49 -1.1784329683087904 2316 50-51 -1.1270468303563348 2316 52-53 -1.0539798439951085 2316 54-55 -1.1392743086444952 2316 56-57 -1.0254329421237145 2316 58-59 -0.992575041016238 2316 60-61 -0.61123599537855 2316 62-63 -0.7923445917214487 2316 64-65 -0.8034635729017872 2316 66-67 -0.5366314533574261 2316 68-69 -0.9047196606292083 2316 70-71 -0.763913407736549 2316 72-73 -0.43178219885570357 2316 74-75 -1.3811510772324738 2316 76-77 -1.380789306166605 2316 78-79 -1.5011823623937133 2316 80-81 -1.058227561244383 2316 82-83 -1.4012045351783762 2316 84-85 -1.7502574993827515 2316 86-87 -1.3509490879636097 2316 88-89 -1.208136795679188 2316 90-91 -0.9535899235369278 2316 92-93 -0.9762738275329887 2316 94-95 -0.8207937439462647 2316 96-97 -0.9036365782716054 2316 98-99 -0.9363704287967956 2316 100-101 -0.916258147944756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 7.0 5 20.0 6 43.0 7 95.0 8 243.0 9 480.0 10 776.0 11 1172.0 12 1736.0 13 2402.0 14 3270.0 15 4240.0 16 5669.0 17 7213.0 18 6384.0 19 4410.0 20 4158.0 21 4293.0 22 4679.0 23 5114.0 24 5916.0 25 6810.0 26 8076.0 27 9730.0 28 11813.0 29 14211.0 30 17025.0 31 20419.0 32 25053.0 33 30978.0 34 39098.0 35 50777.0 36 66147.0 37 71364.0 38 28902.0 39 809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.9527899403751 21.823835717538092 16.453926115188487 20.76944822689832 2 15.422884498801558 22.84933303704918 40.01993445791866 21.707848006230595 3 18.686911180002287 27.57473577932963 31.578118494260387 22.160234546407697 4 12.777299566589651 19.421486711841442 38.47579352494861 29.325420196620307 5 12.336338513115571 39.31003833599765 34.58826016702155 13.765362983865225 6 28.772277270442448 39.392448865560816 17.771550245613582 14.063723618383156 7 25.852959767697232 35.532313772697954 21.82519906889046 16.789527390714362 8 25.581997398243487 35.88331359363843 20.10040277606988 18.4342862320482 9 26.28529144634794 17.19855975734198 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19.753841507154455 34-35 20.34817370068582 28.083653159537725 31.933540869797834 19.63463226997862 36-37 20.77469688846121 27.81333572104353 31.710984941243993 19.70098244925127 38-39 20.312309526249642 28.20947932230451 31.784479457153182 19.693731694292666 40-41 20.417037011468466 28.030966578359205 31.77763347514303 19.774362935029295 42-43 20.2338344842762 28.23229414087023 31.43034045041022 20.10353092444335 44-45 20.564447407196468 28.36216623196191 31.122703237957168 19.950683122884456 46-47 20.809220496944658 28.49864896204689 30.54996143743966 20.14216910356879 48-49 20.710544909307632 28.86902867745015 30.100697384672635 20.31972902856958 50-51 20.70553487383672 29.039506457838236 29.765278372579456 20.489680295745583 52-53 20.79291010564512 29.114216247818387 29.700582267066206 20.392291379470286 54-55 20.759335007464554 28.991379087167008 29.58368930195632 20.66559660341212 56-57 20.80664473329378 29.458713122269565 29.323121730219516 20.41152041421714 58-59 20.76682864739797 28.995054284219552 29.454137520022865 20.783979548359607 60-61 20.809974963351134 28.941546274652364 29.723065107152756 20.525413654843742 62-63 20.68481580284416 28.73628809641185 29.978436349475686 20.600459751268307 64-65 20.493362932088978 28.76170628628382 29.842535482910606 20.9023952987166 66-67 20.484920790733497 29.07000576028167 29.46567454624298 20.97939890274185 68-69 20.35980333569923 28.767978840681636 29.59996203058695 21.272255793032183 70-71 20.421268140720432 28.965825223448803 29.52177749396478 21.091129141865988 72-73 20.587854586404852 28.670019178785545 29.359506227172606 21.382620007636994 74-75 20.32821827140678 28.994699406514744 29.17645561373192 21.500626708346545 76-77 20.387760957688016 28.899452681901828 29.2368612374955 21.475925122914656 78-79 20.320720056006277 28.762112489334324 29.174285008505574 21.74288244615383 80-81 20.007378338320546 29.012618684455223 29.03440851399835 21.945594463225888 82-83 20.171945613488095 28.814753360858152 29.094604610317383 21.918696415336367 84-85 19.893977608864972 28.664773769275083 29.08333998580327 22.357908636056674 86-87 19.919957713509024 28.76409100225456 29.074012146578788 22.241939137657628 88-89 19.902250756688307 28.607132609760257 28.889852502410836 22.60076413114061 90-91 19.6992627190404 28.924389599322588 28.855461650063912 22.520886031573102 92-93 19.78760928305998 29.080700921726095 28.413110191841994 22.718579603371932 94-95 19.62233541085532 29.009045742158595 28.225282773826244 23.14333607315984 96-97 19.9904858576913 29.12373886376115 27.789170058282213 23.09660522026534 98-99 19.730863824646185 29.180510010355544 27.614558163617538 23.474068001380736 100-101 19.74057774038358 29.07395422087028 27.443315427264686 23.74215261148146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 541.0 1 387.5 2 223.0 3 216.0 4 230.5 5 255.5 6 369.0 7 686.5 8 1038.5 9 1150.5 10 1066.5 11 969.5 12 955.0 13 977.5 14 1048.0 15 1126.0 16 1163.5 17 1262.5 18 1332.0 19 1378.5 20 1476.0 21 1699.0 22 1943.5 23 2225.5 24 2605.0 25 3177.5 26 4027.0 27 4874.0 28 5583.0 29 6348.5 30 7286.0 31 8313.0 32 9657.0 33 10862.0 34 12235.5 35 13489.5 36 14520.0 37 15666.5 38 16219.0 39 16255.0 40 16318.5 41 16660.0 42 16689.5 43 16929.5 44 17377.5 45 17526.0 46 17142.5 47 16500.0 48 16679.5 49 16178.0 50 14906.5 51 14063.5 52 13165.0 53 12304.0 54 11249.5 55 9868.5 56 8573.5 57 7349.5 58 6339.5 59 5557.5 60 4661.5 61 3621.0 62 2725.0 63 2040.0 64 1456.5 65 1017.5 66 688.0 67 487.0 68 329.5 69 196.0 70 118.5 71 92.0 72 64.0 73 34.0 74 17.5 75 13.0 76 12.0 77 7.0 78 3.5 79 2.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029339874399449448 2 0.0030202811881786195 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009168710749827952 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.393359264604677E-4 20-21 5.393359264604677E-4 22-23 0.0015101405940893097 24-25 0.009492312305704232 26-27 0.005285492079312584 28-29 3.2360155587628063E-4 30-31 0.0024809452617181515 32-33 0.0022652108911339645 34-35 0.0 36-37 0.010463116973333074 38-39 0.01089458571450145 40-41 2.157343705841871E-4 42-43 0.0 44-45 0.0 46-47 1.0786718529209355E-4 48-49 0.0033438827440549 50-51 0.0057169608204809584 52-53 0.0 54-55 0.001941609335257684 56-57 0.0017258749646734968 58-59 1.0786718529209355E-4 60-61 0.0026966796323023385 62-63 0.0046382889675600225 64-65 0.0 66-67 0.0030202811881786195 68-69 4.314687411683742E-4 70-71 8.629374823367484E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0029124140028865255 80-81 0.0030202811881786195 82-83 0.015640741867353567 84-85 0.009923781046872606 86-87 0.006040562376357239 88-89 0.0 90-91 0.001186539038213029 92-93 1.0786718529209355E-4 94-95 0.0 96-97 0.0026966796323023385 98-99 0.002804546817594432 100-101 6.472031117525613E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 463533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.76853561809048 #Duplication Level Percentage of deduplicated Percentage of total 1 85.92131079221303 58.22761410775112 2 8.475801045873743 11.487852501382868 3 2.256838950451538 4.588280123939071 4 0.9628423625084445 2.610016677530397 5 0.5299049146910594 1.795544004272113 6 0.3450018091326183 1.40281604343057 7 0.2547423949888928 1.208446334776972 8 0.19704040538061673 1.068251178419145 9 0.13191675003468115 0.8045824476013227 >10 0.855463054039703 10.560184953990417 >50 0.05161418611305227 2.3770047015849305 >100 0.015293091990658614 1.5627904316429877 >500 0.0012744243325548835 0.5469118766719655 >1k 9.558182494161627E-4 1.7597046170061266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3519 0.7591692500857544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2664 0.5747163632362744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.424349506939096 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 774 0.1669784028321608 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.15273993437360447 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 539 0.11628082574487685 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 512 0.11045599773910379 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.157343705841871E-4 0.0 5 0.0 0.0 0.0 2.157343705841871E-4 0.0 6 0.0 0.0 0.0 2.157343705841871E-4 0.0 7 0.0 0.0 0.0 2.157343705841871E-4 0.0 8 0.0 0.0 0.0 2.157343705841871E-4 0.0 9 0.0 0.0 0.0 4.314687411683742E-4 0.0 10-11 0.0 0.0 0.0 4.314687411683742E-4 0.0 12-13 0.0 0.0 0.0 4.314687411683742E-4 0.0 14-15 0.0 0.0 0.0 6.472031117525613E-4 0.0 16-17 0.0 0.0 0.0 6.472031117525613E-4 0.0 18-19 0.0 0.0 0.0 7.550702970446548E-4 0.0 20-21 0.0 0.0 0.0 0.0010786718529209355 0.0 22-23 0.0 0.0 0.0 0.002157343705841871 0.0 24-25 0.0 0.0 0.0 0.0026966796323023385 0.0 26-27 0.0 0.0 0.0 0.0030202811881786195 0.0 28-29 0.0 0.0 0.0 0.006472031117525613 0.0 30-31 0.0 0.0 0.0 0.016935148090858686 0.0 32-33 0.0 0.0 0.0 0.03462536647876203 0.0 34-35 0.0 0.0 0.0 0.06342590495175102 0.0 36-37 0.0 0.0 0.0 0.10096368543339956 0.0 38-39 0.0 0.0 0.0 0.16449745757044265 0.0 40-41 0.0 0.0 0.0 0.24032808883078444 0.0 42-43 0.0 0.0 0.0 0.32834771202913277 0.0 44-45 0.0 0.0 0.0 0.43945091287998916 0.0 46-47 0.0 0.0 0.0 0.5884154957683703 0.0 48-49 0.0 0.0 0.0 0.7514028127447236 0.0 50-51 0.0 0.0 0.0 0.9215093639503552 0.0 52-53 0.0 0.0 0.0 1.0973328759764676 0.0 54-55 0.0 0.0 0.0 1.2816778956406556 0.0 56-57 0.0 0.0 0.0 1.51736769550388 0.0 58-59 0.0 0.0 0.0 1.7633048779698532 0.0 60-61 0.0 0.0 0.0 2.0485057158821487 0.0 62-63 0.0 0.0 0.0 2.372970209240766 0.0 64-65 0.0 0.0 0.0 2.709407960166806 0.0 66-67 0.0 0.0 0.0 3.0758327886040475 0.0 68-69 0.0 0.0 0.0 3.506438592290085 0.0 70-71 0.0 0.0 0.0 4.0432935734888344 0.0 72-73 0.0 0.0 0.0 4.555339102070403 0.0 74-75 0.0 0.0 0.0 5.06738463065197 0.0 76-77 0.0 0.0 0.0 5.709517984695804 0.0 78-79 0.0 0.0 0.0 6.337952206207541 0.0 80-81 0.0 0.0 0.0 7.029380863929861 0.0 82-83 0.0 0.0 0.0 7.689312303546888 0.0 84-85 0.0 0.0 0.0 8.36434514910481 0.0 86-87 0.0 0.0 0.0 9.019206831013111 0.0 88-89 0.0 0.0 0.0 9.700711707688557 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1845 0.0 44.537777 1 GTATCAA 3120 0.0 37.90736 1 TATCAAC 3975 0.0 29.511534 2 ATCAACG 4035 0.0 29.072702 3 TCAACGC 4080 0.0 28.752045 4 CAACGCA 4190 0.0 28.003258 5 AACGCAG 4305 0.0 27.255207 6 ACGCAGA 4740 0.0 24.753937 7 CGCAGAG 4795 0.0 24.172796 8 TAATACG 100 0.0016535281 23.746628 3 GAGTACT 2770 0.0 23.6609 12-13 GTGCATA 205 2.6615453E-8 23.169943 1 ACTTACC 125 2.2519568E-4 22.801683 8 GCAGAGT 4925 0.0 22.666647 9 CTAGAGT 170 3.1461423E-6 22.349768 3 GTACTTT 2980 0.0 21.913836 14-15 CTAAGCC 110 0.0028771732 21.587843 3 AGTACTT 2975 0.0 21.471743 12-13 GTACATG 2435 0.0 21.067043 1 TATTGGG 160 4.9864997E-5 20.7783 2 >>END_MODULE