FastQCFastQC Report
Thu 26 May 2016
SRR936784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488192
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53631.0985431961195595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.7241003539590981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.49529693235448347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.17923276088096488No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.14031364708966965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.13437336130047195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.053.5799261
GTATCAA32400.045.3473551
TATCAAC41300.035.8054242
ATCAACG41500.035.4037133
TCAACGC41800.035.1496244
CAACGCA42700.034.4087645
AACGCAG43850.033.4994936
ACGCAGA48800.029.900537
CGCAGAG49050.029.7481278
GCAGAGT51200.027.6577939
GAGTACT30100.025.42258312-13
CTTACAC2151.6370905E-924.3272343
AGTACTT32000.023.46753112-13
GTACTTT33600.022.7732314-15
CAGAGTA48950.022.61905510-11
GTGGTAT8650.022.5375271
GTACATG26100.022.043641
TACATGG26350.021.6540492
TAGGGTC1100.002857976121.6130374
AGAGTAC46850.021.55363710-11
Produced by FastQC (version 0.11.5)