FastQCFastQC Report
Thu 26 May 2016
SRR936784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488192
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.7482711719979025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.6052946381751442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.417458704771893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7610.15588129260618772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.14133783429470373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.10692514420555846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.10590095700052438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.050.134271
GTATCAA31500.036.6546751
GATATAC953.4864137E-530.0004543
TATCAAC41250.027.982242
ATCAACG41850.027.5810623
TCAACGC42500.027.1592334
CAACGCA42850.026.8265445
AACGCAG44050.026.095746
GTGTAAG2653.092282E-1123.30941
ACGCAGA49150.023.1946537
GAGTACT29700.022.9542112-13
CGCAGAG49750.022.8241168
GCAGAGT50200.021.862389
GGCCTAA2205.6215868E-821.5912346
AGTACTT31250.021.43561612-13
GTACTTT32200.021.31631514-15
GGTTAAC1604.9811657E-520.7815632
AGAGTAC46450.020.55888210-11
CTAAGGG1404.8457456E-420.357452
CAGAGTA48950.019.60594710-11
Produced by FastQC (version 0.11.5)