##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488192 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51533822758259 31.0 31.0 34.0 30.0 34.0 2 31.674793933534346 31.0 31.0 34.0 30.0 34.0 3 31.766737267304666 31.0 31.0 34.0 30.0 34.0 4 35.591230499475614 37.0 35.0 37.0 33.0 37.0 5 35.349964767960145 37.0 35.0 37.0 33.0 37.0 6 35.46986636405349 37.0 35.0 37.0 33.0 37.0 7 35.41162698282643 37.0 35.0 37.0 33.0 37.0 8 35.3734944448086 37.0 35.0 37.0 33.0 37.0 9 36.91676635422129 39.0 37.0 39.0 33.0 39.0 10-11 36.803817965062926 39.0 37.0 39.0 32.0 39.0 12-13 36.67027931633456 38.0 35.0 39.0 32.0 39.0 14-15 37.62788718373099 39.0 36.5 41.0 32.0 41.0 16-17 37.626214686025165 39.0 36.5 40.5 32.0 41.0 18-19 37.78412182092291 39.5 37.0 41.0 32.0 41.0 20-21 37.79739631128736 39.5 37.0 41.0 32.0 41.0 22-23 37.68687934255375 39.0 37.0 41.0 32.0 41.0 24-25 37.561001614119036 39.0 36.0 41.0 32.0 41.0 26-27 37.294252056567906 39.0 36.0 40.5 31.5 41.0 28-29 37.076279414656526 39.0 36.0 40.0 31.0 41.0 30-31 36.98720073249869 39.0 36.0 40.0 31.0 41.0 32-33 36.818635905545364 39.0 35.0 40.0 31.0 41.0 34-35 36.60561725714473 38.5 35.0 40.0 30.0 41.0 36-37 36.39317829870215 38.0 35.0 40.0 30.0 41.0 38-39 36.1615409511012 38.0 35.0 40.0 30.0 41.0 40-41 35.70022245346094 38.0 35.0 40.0 28.5 41.0 42-43 35.45954562958836 38.0 34.0 40.0 27.0 41.0 44-45 35.16293896663608 38.0 34.0 40.0 27.0 41.0 46-47 34.663795187139485 38.0 33.5 40.0 24.5 41.0 48-49 34.19453104516256 38.0 33.0 40.0 23.5 41.0 50-51 33.854073807026744 38.0 33.0 40.0 23.0 41.0 52-53 33.54962596683272 37.5 33.0 40.0 21.5 41.0 54-55 33.18498459622444 37.0 32.0 40.0 19.5 41.0 56-57 32.86903718209229 37.0 31.5 39.5 18.5 40.0 58-59 32.47953673964342 36.0 31.0 39.0 17.0 40.0 60-61 32.01705169277661 36.0 31.0 39.0 13.5 40.0 62-63 31.58302163902727 35.5 30.0 39.0 10.5 40.0 64-65 31.208924972142107 35.0 29.5 38.0 8.0 40.0 66-67 30.814208344258 35.0 29.0 38.0 7.5 40.0 68-69 30.265750975026222 34.0 28.5 38.0 6.5 40.0 70-71 29.764638707721552 34.0 28.0 37.0 2.0 39.0 72-73 29.28734493805716 34.0 28.0 36.5 2.0 39.0 74-75 28.736176545293656 33.5 26.5 36.0 2.0 39.0 76-77 27.486199077412167 31.5 26.0 34.5 2.0 37.0 78-79 28.142812663869954 33.0 26.0 35.0 2.0 37.0 80-81 27.96900604680126 33.0 26.0 35.0 2.0 37.0 82-83 27.538477689105925 33.0 26.0 35.0 2.0 36.5 84-85 26.987167958508127 32.0 25.5 35.0 2.0 36.0 86-87 26.462045670555845 32.0 25.0 34.5 2.0 35.5 88-89 25.922254973453065 32.0 23.5 34.0 2.0 35.0 90-91 25.271215013765076 31.0 20.5 34.0 2.0 35.0 92-93 24.53791131358154 31.0 18.5 34.0 2.0 35.0 94-95 23.733058919441532 31.0 9.5 34.0 2.0 35.0 96-97 22.66848903710016 30.0 2.0 34.0 2.0 35.0 98-99 21.45183964505768 29.0 2.0 34.0 2.0 35.0 100-101 18.30225812794966 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37984774942358257 1101 2 0.5249212178457228 1101 3 0.5431313506962638 1101 4 0.5184409696270791 1101 5 0.3770822584500664 1101 6 0.5099423314451315 1101 7 0.5148728422481881 1101 8 0.5743731760999822 1101 9 0.6465978256242408 1101 10-11 0.661763849355431 1101 12-13 0.7670422141685975 1101 14-15 1.0369480082724039 1101 16-17 1.0551874635708671 1101 18-19 0.7311576227973333 1101 20-21 0.7186738124427521 1101 22-23 0.8129337282156399 1101 24-25 0.7586473371068365 1101 26-27 0.8034161517711524 1101 28-29 1.0727670874741975 1101 30-31 1.163540735446773 1101 32-33 1.2007274847437301 1101 34-35 1.184901379699781 1101 36-37 1.4034503712319264 1101 38-39 1.4101457310601901 1101 40-41 1.5318499215709096 1101 42-43 1.3840352283110136 1101 44-45 1.441302799869007 1101 46-47 1.5479418231629865 1101 48-49 1.6553380100967985 1101 50-51 1.627052033943741 1101 52-53 1.7858584443249939 1101 54-55 1.6513300568376437 1101 56-57 1.6753186885557518 1101 58-59 1.8515426252396843 1101 60-61 1.9821249972103274 1101 62-63 2.1150574855900928 1101 64-65 2.1288227817851038 1101 66-67 2.0133296487707817 1101 68-69 1.9774286373396315 1101 70-71 1.7362376959544612 1101 72-73 1.8697006612972373 1101 74-75 1.8944119361295364 1101 76-77 1.9789911818877783 1101 78-79 2.1286466700053523 1101 80-81 1.9878930352484012 1101 82-83 2.434655091430674 1101 84-85 2.2666113072772305 1101 86-87 1.791980491627843 1101 88-89 2.2701531467156855 1101 90-91 2.41637762255888 1101 92-93 2.238456672983343 1101 94-95 2.2044927062186623 1101 96-97 2.139053867666629 1101 98-99 1.9722571969609959 1101 100-101 2.2151834309081018 1102 1 0.08290714628904539 1102 2 0.24196425431168578 1102 3 0.2473637430781075 1102 4 0.19122002434275487 1102 5 0.1076668345036822 1102 6 0.20942228364003057 1102 7 0.3549864819242643 1102 8 0.4387872019523087 1102 9 0.4480096307202146 1102 10-11 0.559470327335184 1102 12-13 0.26498798755212505 1102 14-15 0.40963103173987037 1102 16-17 0.512185545105126 1102 18-19 0.5882292257256623 1102 20-21 0.5518428652259288 1102 22-23 0.34022436228098485 1102 24-25 0.40408447631489963 1102 26-27 0.6520379225247552 1102 28-29 0.34667449586124377 1102 30-31 0.226940522331887 1102 32-33 0.4008311812180523 1102 34-35 0.8084300071600765 1102 36-37 0.7554086745611102 1102 38-39 0.9336936295466813 1102 40-41 1.0656346755441461 1102 42-43 1.0174495683296456 1102 44-45 0.973552079499683 1102 46-47 0.8313584303611776 1102 48-49 1.0482086480703288 1102 50-51 0.9228873870461598 1102 52-53 1.1005305507831054 1102 54-55 1.3205542279945917 1102 56-57 1.2245955662392625 1102 58-59 0.9387788361996563 1102 60-61 1.1440863666299634 1102 62-63 1.2008297463243807 1102 64-65 1.3653910924448454 1102 66-67 1.2632162897931245 1102 68-69 1.2800196457055222 1102 70-71 0.8670315542730087 1102 72-73 0.9106734540629944 1102 74-75 1.3210492446203403 1102 76-77 1.423885223631956 1102 78-79 1.249721885306439 1102 80-81 1.7793592094692734 1102 82-83 1.9214717122319378 1102 84-85 1.8344482884304902 1102 86-87 1.7390766664640935 1102 88-89 1.6806988784262984 1102 90-91 1.5299676366045993 1102 92-93 1.5105352231975964 1102 94-95 1.6431576721407914 1102 96-97 2.111165399945328 1102 98-99 1.8505647150363522 1102 100-101 1.8215578761238689 1103 1 0.19069217967071594 1103 2 0.3062635135330183 1103 3 0.29402402172499364 1103 4 0.13176755322142242 1103 5 0.06986536172377811 1103 6 0.23122322977403087 1103 7 0.3393151089693589 1103 8 0.3105946397285493 1103 9 0.2694263813515505 1103 10-11 0.34706516474240345 1103 12-13 0.3825520856618638 1103 14-15 0.5367483585730284 1103 16-17 0.40997783815431177 1103 18-19 0.28312204048345535 1103 20-21 0.4098455057699866 1103 22-23 0.4854730287513789 1103 24-25 0.5881952219028648 1103 26-27 0.6108685674648342 1103 28-29 0.5518465060942717 1103 30-31 0.6522134913164663 1103 32-33 0.8365488368391709 1103 34-35 0.7699162823654788 1103 36-37 0.8620416700242828 1103 38-39 0.5910418942667164 1103 40-41 0.7535389174209044 1103 42-43 0.40374708303641427 1103 44-45 0.9309595918840969 1103 46-47 0.686630009585727 1103 48-49 0.8238904076528115 1103 50-51 0.5566805907538921 1103 52-53 0.3612339887934084 1103 54-55 1.0102203564340115 1103 56-57 0.6370123406428014 1103 58-59 0.4928208435251591 1103 60-61 0.4500626090479969 1103 62-63 1.0314198274607662 1103 64-65 1.1582437029079102 1103 66-67 0.9440633851032381 1103 68-69 0.8417291053997396 1103 70-71 0.846027434365979 1103 72-73 1.083020612460892 1103 74-75 0.7633801464831684 1103 76-77 0.9023114849763267 1103 78-79 1.0773820414350048 1103 80-81 1.1621946673677748 1103 82-83 1.2672755512611538 1103 84-85 1.2426418381153255 1103 86-87 1.0149655185771707 1103 88-89 1.4052388404273302 1103 90-91 1.3594605387709784 1103 92-93 1.4618890506149995 1103 94-95 1.2981575167071036 1103 96-97 1.6178828781891852 1103 98-99 1.3655549227140469 1103 100-101 1.2429536889231905 1104 1 -0.009349265380055982 1104 2 0.11447340131740447 1104 3 -0.012396193412495649 1104 4 0.0019189532477952298 1104 5 -0.39511671015198857 1104 6 -0.004727839947847201 1104 7 0.07056907293728187 1104 8 -0.08115214357034972 1104 9 -0.09378611244738977 1104 10-11 0.13767503494403854 1104 12-13 0.15977237866581362 1104 14-15 0.16433788930202553 1104 16-17 -0.030397804714944243 1104 18-19 0.19045781446977372 1104 20-21 0.21471153150360323 1104 22-23 0.21964541504437562 1104 24-25 0.4294638172296317 1104 26-27 0.2456623155417219 1104 28-29 0.40736340751156064 1104 30-31 0.40018205587341527 1104 32-33 0.46078194641108894 1104 34-35 0.7271462458812721 1104 36-37 0.5481723349661678 1104 38-39 0.6238086025877507 1104 40-41 0.5139237284377529 1104 42-43 0.4147676988093991 1104 44-45 0.3311680730485733 1104 46-47 0.4984443608764906 1104 48-49 0.1791404280613591 1104 50-51 0.18919172126184236 1104 52-53 0.10013173882951776 1104 54-55 0.09985469805426561 1104 56-57 0.16733944256623445 1104 58-59 -0.13930566435253766 1104 60-61 -0.23619992650997546 1104 62-63 0.043459300731846895 1104 64-65 0.10470894244146223 1104 66-67 0.13772951382151533 1104 68-69 0.17508389387299061 1104 70-71 0.21494333231068197 1104 72-73 -0.005786398032242346 1104 74-75 0.058545832492949756 1104 76-77 0.4464096469511212 1104 78-79 0.2777971198980218 1104 80-81 0.21222857695476804 1104 82-83 0.5765325545328004 1104 84-85 0.6071793176282867 1104 86-87 0.46399909534784456 1104 88-89 0.04367472932947436 1104 90-91 0.5496446552692476 1104 92-93 0.3468507767385667 1104 94-95 0.5208069229753569 1104 96-97 0.836158575690547 1104 98-99 0.7465730894613003 1104 100-101 1.4822847281264409 1105 1 0.06567262161570397 1105 2 0.17427234706826766 1105 3 0.3244921186256562 1105 4 0.0640229210693235 1105 5 0.12723863670332491 1105 6 0.11589552618026744 1105 7 0.12292031789409208 1105 8 0.031359259095268044 1105 9 0.024691409761025795 1105 10-11 0.15669376286572145 1105 12-13 0.36383111540352076 1105 14-15 0.5076683247767093 1105 16-17 0.4212946875863466 1105 18-19 0.5243186805213682 1105 20-21 0.3785135016393255 1105 22-23 0.26095214521904353 1105 24-25 0.21643906502714572 1105 26-27 0.14644075792229216 1105 28-29 0.10526011313606176 1105 30-31 0.06620945900432673 1105 32-33 0.17172360761876604 1105 34-35 0.29612854649533915 1105 36-37 0.23937131377709875 1105 38-39 0.10383174464200096 1105 40-41 0.10617511971601346 1105 42-43 0.1053559845219354 1105 44-45 -0.05829800669967966 1105 46-47 -0.43394931117978786 1105 48-49 -0.0027604039505888522 1105 50-51 -0.14459414739169318 1105 52-53 0.18249571803839615 1105 54-55 0.5898126995113557 1105 56-57 0.4359801223793802 1105 58-59 0.2372149461568931 1105 60-61 0.29038282470069987 1105 62-63 0.5214575130387686 1105 64-65 0.40657959182709646 1105 66-67 0.670270731779258 1105 68-69 0.4540068700146378 1105 70-71 0.33620034992435777 1105 72-73 0.3349160673924274 1105 74-75 0.04058931261711152 1105 76-77 0.06740493895119215 1105 78-79 0.32050431168932647 1105 80-81 0.12731315565305934 1105 82-83 0.444662639815558 1105 84-85 0.25475889139817554 1105 86-87 0.31206355025451415 1105 88-89 0.622291899754174 1105 90-91 0.41729873665826034 1105 92-93 0.6165720313072889 1105 94-95 0.8125131290865717 1105 96-97 0.6024376027447147 1105 98-99 0.9228812583615635 1105 100-101 1.5787252597814678 1106 1 0.023244820641451724 1106 2 -0.02488388118504048 1106 3 0.03798666745423418 1106 4 0.16721098648337573 1106 5 -0.0184569675648234 1106 6 0.13119795021181346 1106 7 0.25016747632600556 1106 8 0.09500458500573217 1106 9 0.524727654251862 1106 10-11 0.2845317064982922 1106 12-13 0.24291224507181397 1106 14-15 0.20756756509218377 1106 16-17 0.273167077983004 1106 18-19 0.301512921996661 1106 20-21 0.40223189644331825 1106 22-23 0.17265331664098227 1106 24-25 -0.01669637338621044 1106 26-27 0.24433350322500758 1106 28-29 0.40268965384587574 1106 30-31 0.40845531263457246 1106 32-33 0.48644177045801484 1106 34-35 0.48462526999336575 1106 36-37 0.4758666164910892 1106 38-39 0.3359450884137445 1106 40-41 0.31918399236737116 1106 42-43 0.5415801784444554 1106 44-45 0.4812882261929232 1106 46-47 0.46204712236230705 1106 48-49 0.5385510667058782 1106 50-51 1.204755626442683 1106 52-53 0.44013968115600477 1106 54-55 0.6195730509379374 1106 56-57 0.7652540727389301 1106 58-59 0.5138437735751253 1106 60-61 0.6438862171850417 1106 62-63 0.6285491101160119 1106 64-65 1.34876238004329 1106 66-67 1.5047566399212222 1106 68-69 1.1186220984999053 1106 70-71 1.0891796470711874 1106 72-73 1.3270836329224664 1106 74-75 1.156771027996374 1106 76-77 1.1954168561397793 1106 78-79 1.59624877353113 1106 80-81 1.3045632136442435 1106 82-83 1.091032391613286 1106 84-85 0.736730881655653 1106 86-87 1.480031394142241 1106 88-89 1.4859401252239053 1106 90-91 1.1872658266605818 1106 92-93 1.8741652521377183 1106 94-95 1.392019044187478 1106 96-97 1.227053411141835 1106 98-99 1.0218867095329855 1106 100-101 1.0989605590159819 1107 1 0.20730489816083164 1107 2 0.04905022734209297 1107 3 0.28714687417258133 1107 4 -0.005705680183289985 1107 5 -0.5120777685984166 1107 6 -0.21025876813444455 1107 7 -0.30852115416494996 1107 8 -0.05299283101494012 1107 9 0.4498407033474692 1107 10-11 0.39797647394015456 1107 12-13 0.6142462243999844 1107 14-15 0.3437998002343008 1107 16-17 0.2595769617039352 1107 18-19 0.18918249564006828 1107 20-21 -0.149835287019215 1107 22-23 0.2387547378296091 1107 24-25 0.4044518824277432 1107 26-27 0.0884468753180272 1107 28-29 0.020276863148204427 1107 30-31 -0.16585831785638305 1107 32-33 -0.32402334582105397 1107 34-35 0.05285039919233725 1107 36-37 -0.2591624532763461 1107 38-39 -0.5096702216637681 1107 40-41 -0.5611939146093761 1107 42-43 0.10614736190698437 1107 44-45 0.544644169345375 1107 46-47 0.12340855647083515 1107 48-49 0.16938117006504427 1107 50-51 0.2688786884581944 1107 52-53 0.6329933633265483 1107 54-55 0.6398976633410385 1107 56-57 0.8634452872092062 1107 58-59 1.1283544324898536 1107 60-61 0.878987961371088 1107 62-63 1.5230985287206664 1107 64-65 1.467496230172486 1107 66-67 1.453739198060756 1107 68-69 0.6305568530218686 1107 70-71 0.5871932129626636 1107 72-73 1.5420141884780243 1107 74-75 1.042696415593273 1107 76-77 0.9014972824963721 1107 78-79 1.37474522055955 1107 80-81 1.8220696735925621 1107 82-83 2.086639626755403 1107 84-85 1.9920118894075927 1107 86-87 1.856783655124154 1107 88-89 1.6408819856890204 1107 90-91 1.5929748059887459 1107 92-93 1.6016281074348804 1107 94-95 1.782329121706855 1107 96-97 1.761436486077237 1107 98-99 2.009063712116962 1107 100-101 2.5939944737446687 1108 1 -0.0884649494734937 1108 2 -0.13623445589768224 1108 3 -0.4323198459557247 1108 4 -0.28070568018328856 1108 5 0.0884635305194692 1108 6 0.06163377396659797 1108 7 -0.19212179570464372 1108 8 0.13858696048465902 1108 9 0.11249106421354327 1108 10-11 -0.34962777626032704 1108 12-13 -0.3331174484147752 1108 14-15 -0.812966438739231 1108 16-17 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62-63 -1.576046478119281 2316 64-65 -2.095191868322722 2316 66-67 -2.1764933600787764 2316 68-69 -1.5370560060752574 2316 70-71 -1.7528546666543008 2316 72-73 -1.6623975762278604 2316 74-75 -1.3098343641604906 2316 76-77 -1.6383453987621799 2316 78-79 -1.4010755728741024 2316 80-81 -1.715208028185824 2316 82-83 -1.7012878698246219 2316 84-85 -1.326626961481601 2316 86-87 -1.6290576581453422 2316 88-89 -2.426559874776096 2316 90-91 -1.8784000230126203 2316 92-93 -1.2259368720452848 2316 94-95 -1.6172946813027202 2316 96-97 -1.6903076999692743 2316 98-99 -1.7461688460225702 2316 100-101 -1.8112722841212694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 20.0 6 48.0 7 132.0 8 303.0 9 541.0 10 874.0 11 1356.0 12 1995.0 13 2620.0 14 3432.0 15 4857.0 16 5885.0 17 7589.0 18 6423.0 19 4799.0 20 4503.0 21 4757.0 22 5069.0 23 5708.0 24 6553.0 25 7712.0 26 9081.0 27 10897.0 28 13223.0 29 15851.0 30 18630.0 31 22478.0 32 26713.0 33 33336.0 34 41777.0 35 53066.0 36 68845.0 37 72061.0 38 26412.0 39 634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.75005890246776 22.024196109363956 16.49269097204438 20.733054016123912 2 15.502873975262919 22.974185433882184 39.988200928357514 21.534739662497387 3 18.60845732826429 27.673538280020978 31.577944743051916 22.14005964866282 4 12.753179077084425 19.551324069218666 38.35273826691138 29.34275858678553 5 12.235090723617638 39.568320271371974 34.50467649625348 13.691912508756909 6 28.714931830099633 39.443702477713686 17.71966767173571 14.121698020450971 7 25.75958999653824 35.53138013605331 21.848825562126297 16.860204305282153 8 25.568423898793917 35.836924816465654 20.249205230728894 18.345446054011536 9 26.22652292452308 17.149505621798113 21.31387638803824 35.31009506564056 10-11 23.71454263896172 28.589264060041952 27.85195169113791 19.844241609858415 12-13 25.026503096893272 26.44840064693163 28.610438788857124 19.914657467317966 14-15 22.232350907740763 27.232939850971754 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19.627406936237378 40-41 20.384401878788253 28.025326360595425 31.72380665605598 19.866465104560344 42-43 20.33206197561615 28.12981367986366 31.532982924750918 20.00514141976927 44-45 20.42700850288926 28.436848994340284 31.128850215183935 20.00729228758652 46-47 20.77541530961306 28.53929822405211 30.55101497367828 20.13427149265655 48-49 20.741758101073042 28.87749991550447 30.0450745250583 20.33566745836419 50-51 20.708776177614997 29.028257024311756 29.76866091263562 20.49430588543763 52-53 20.721419200086853 29.079939081157868 29.847160073782675 20.35148164497261 54-55 20.593844225222863 29.041442711064498 29.68033068956476 20.684382374147877 56-57 20.798271991347665 29.42336211982171 29.379014813843735 20.39935107498689 58-59 20.767179239273155 28.982611314014388 29.491530978653845 20.75867846805861 60-61 20.639105252646296 28.998069369549295 29.777490999779793 20.585334378024612 62-63 20.53095913083377 28.74575986497116 30.160264813843735 20.563016190351338 64-65 20.379502715635155 28.764531951088866 29.907628461372227 20.94833687190375 66-67 20.481798144992133 29.004367134242266 29.554560500786575 20.959274219979022 68-69 20.360452814110854 28.873300743663094 29.569953594030213 21.196292848195842 70-71 20.371720788465122 28.959480856799313 29.51141873331845 21.157379621417117 72-73 20.557837802406244 28.66945862100398 29.431152106204877 21.341551470384903 74-75 20.263414764050307 28.950530096862643 29.25350111796102 21.532554021126032 76-77 20.31835867687168 28.7952575987087 29.328552422563686 21.55783130185593 78-79 20.32843736205436 28.73632321808802 29.152554177109142 21.782685242748475 80-81 19.972202505871376 29.133904236915768 29.072143408030136 21.82174984918272 82-83 20.03713710412523 28.848340096396697 29.180689525206322 21.93383327427175 84-85 19.856627667234434 28.813045468838176 29.0192238556485 22.311103008278888 86-87 19.930764661299904 28.77435015055614 29.00315450951474 22.291730678629225 88-89 19.991457867519106 28.52334798332953 28.9541418588049 22.53105229034647 90-91 19.763998807805798 28.89872597047578 28.8651314947186 22.472143726999818 92-93 19.760667933927635 29.07575298243314 28.498418654955426 22.665160428683798 94-95 19.580615823282642 28.926528906659676 28.347555879653907 23.145299390403775 96-97 19.935824035363353 29.124888106860254 27.796611558228474 23.142676299547922 98-99 19.67051425176931 29.314808935137293 27.63204522875549 23.382631584337904 100-101 19.66219288865946 29.110385965774775 27.59884714182122 23.628574003744536 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 494.0 1 349.0 2 230.5 3 249.5 4 248.5 5 275.0 6 361.5 7 668.0 8 1077.0 9 1251.0 10 1141.5 11 1012.0 12 994.5 13 1062.5 14 1163.0 15 1164.5 16 1201.0 17 1295.0 18 1369.5 19 1466.5 20 1592.5 21 1805.5 22 2068.5 23 2405.0 24 2827.0 25 3418.0 26 4293.0 27 5151.0 28 5834.5 29 6656.0 30 7670.0 31 8909.0 32 10173.5 33 11511.0 34 13181.5 35 14297.0 36 15460.5 37 16619.5 38 17078.0 39 17151.0 40 17084.0 41 17350.5 42 17512.0 43 17793.5 44 18277.5 45 18390.5 46 17978.0 47 17381.5 48 17498.5 49 17089.5 50 15849.5 51 14756.5 52 13759.0 53 12873.0 54 11854.0 55 10502.0 56 8924.0 57 7668.0 58 6735.5 59 5809.0 60 4820.5 61 3766.5 62 2826.0 63 2093.0 64 1468.0 65 1028.0 66 711.0 67 532.5 68 378.0 69 199.5 70 117.0 71 86.0 72 55.0 73 36.0 74 22.5 75 16.5 76 10.0 77 6.0 78 2.0 79 1.0 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01986923177766125 2 0.0036870739381227057 3 0.0 4 0.0 5 0.001229024646040902 6 0.0 7 2.0483744100681698E-4 8 0.0 9 6.14512323020451E-4 10-11 0.0 12-13 0.008500753801782905 14-15 0.006042704509701101 16-17 0.0 18-19 2.0483744100681698E-4 20-21 4.0967488201363396E-4 22-23 0.00204837441006817 24-25 0.0011266059255374934 26-27 4.0967488201363396E-4 28-29 1.0241872050340849E-4 30-31 0.0 32-33 4.0967488201363396E-4 34-35 0.0 36-37 0.002867724174095438 38-39 0.009320103565810173 40-41 0.001024187205034085 42-43 0.0 44-45 0.001024187205034085 46-47 4.0967488201363396E-4 48-49 1.0241872050340849E-4 50-51 0.002560468012585212 52-53 3.072561615102255E-4 54-55 0.0 56-57 0.0 58-59 2.0483744100681698E-4 60-61 0.0019459556895647613 62-63 0.0 64-65 1.0241872050340849E-4 66-67 0.0 68-69 1.0241872050340849E-4 70-71 6.14512323020451E-4 72-73 7.169310435238595E-4 74-75 0.00542819218668065 76-77 1.0241872050340849E-4 78-79 3.072561615102255E-4 80-81 0.0037894926586261144 82-83 2.0483744100681698E-4 84-85 0.004608842422653382 86-87 4.0967488201363396E-4 88-89 0.004813679863660199 90-91 0.0033798177766124803 92-93 0.0 94-95 0.0 96-97 6.14512323020451E-4 98-99 0.001433862087047719 100-101 0.002867724174095438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 488192.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.62196614807247 #Duplication Level Percentage of deduplicated Percentage of total 1 85.79283542422151 58.0148021280386 2 8.546677051529484 11.558862125140694 3 2.3087784398247297 4.683724125036824 4 0.9651235376132397 2.6105420475676184 5 0.5530559631478276 1.8699365808986008 6 0.3338723283070298 1.3546261969533666 7 0.2519500121092763 1.1926148632901954 8 0.18543879874823638 1.0031788937193973 9 0.13455497299479938 0.8188984646018218 >10 0.8574214185610564 10.578145150737898 >50 0.051121493571716844 2.3093566309679554 >100 0.01704049721879513 1.713858220050784 >500 0.0012171783727710755 0.5123672671300803 >1k 9.128837795783066E-4 1.7790873058661343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 0.7482711719979025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2955 0.6052946381751442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.417458704771893 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 761 0.15588129260618772 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 690 0.14133783429470373 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.10692514420555846 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.10590095700052438 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.0483744100681698E-4 0.0 3 0.0 0.0 0.0 2.0483744100681698E-4 0.0 4 0.0 0.0 0.0 2.0483744100681698E-4 0.0 5 0.0 0.0 0.0 2.0483744100681698E-4 0.0 6 0.0 0.0 0.0 2.0483744100681698E-4 0.0 7 0.0 0.0 0.0 2.0483744100681698E-4 0.0 8 0.0 0.0 0.0 2.0483744100681698E-4 0.0 9 0.0 0.0 0.0 2.0483744100681698E-4 0.0 10-11 0.0 0.0 0.0 3.0725616151022544E-4 0.0 12-13 0.0 0.0 0.0 4.0967488201363396E-4 0.0 14-15 0.0 0.0 0.0 4.0967488201363396E-4 0.0 16-17 0.0 0.0 0.0 9.217684845306764E-4 0.0 18-19 0.0 0.0 0.0 0.001024187205034085 0.0 20-21 0.0 0.0 0.0 0.0013314433665443104 0.0 22-23 0.0 0.0 0.0 0.0022532118510749867 0.0 24-25 0.0 0.0 0.0 0.003174980335605663 0.0 26-27 0.0 0.0 0.0 0.004301586261143157 0.0 28-29 0.0 0.0 0.0 0.009729778447823807 0.0 30-31 0.0 0.0 0.0 0.022634537231253277 0.0 32-33 0.0 0.0 0.0 0.042298931567907705 0.0 34-35 0.0 0.0 0.0 0.06964472994231778 0.0 36-37 0.0 0.0 0.0 0.11624524777136863 0.0 38-39 0.0 0.0 0.0 0.19490282511798634 0.0 40-41 0.0 0.0 0.0 0.28646516124803356 0.0 42-43 0.0 0.0 0.0 0.38724518222338755 0.0 44-45 0.0 0.0 0.0 0.5086113660199265 0.0 46-47 0.0 0.0 0.0 0.665926520713162 0.0 48-49 0.0 0.0 0.0 0.8334835474567384 0.0 50-51 0.0 0.0 0.0 1.0225485055060304 0.0 52-53 0.0 0.0 0.0 1.210486857629785 0.0 54-55 0.0 0.0 0.0 1.3997566531200838 0.0 56-57 0.0 0.0 0.0 1.6524236366019927 0.0 58-59 0.0 0.0 0.0 1.9267009701101205 2.0483744100681698E-4 60-61 0.0 0.0 0.0 2.2132685500786575 2.0483744100681698E-4 62-63 0.0 0.0 0.0 2.5291278841111695 2.0483744100681698E-4 64-65 0.0 0.0 0.0 2.857175045883587 2.0483744100681698E-4 66-67 0.0 0.0 0.0 3.22055666622968 2.0483744100681698E-4 68-69 0.0 0.0 0.0 3.6503056174619823 2.0483744100681698E-4 70-71 0.0 0.0 0.0 4.19230548636602 2.0483744100681698E-4 72-73 0.0 0.0 0.0 4.725599764027268 2.0483744100681698E-4 74-75 0.0 0.0 0.0 5.236361923177766 2.0483744100681698E-4 76-77 0.0 0.0 0.0 5.834077576035658 2.0483744100681698E-4 78-79 0.0 0.0 0.0 6.457090652857892 2.0483744100681698E-4 80-81 0.0 0.0 0.0 7.142579149187204 2.0483744100681698E-4 82-83 0.0 0.0 0.0 7.798058960409019 2.0483744100681698E-4 84-85 0.0 0.0 0.0 8.472383816203461 2.0483744100681698E-4 86-87 0.0 0.0 0.0 9.094270287100157 2.0483744100681698E-4 88-89 0.0 0.0 0.0 9.762859694546407 2.0483744100681698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1905 0.0 50.13427 1 GTATCAA 3150 0.0 36.654675 1 GATATAC 95 3.4864137E-5 30.000454 3 TATCAAC 4125 0.0 27.98224 2 ATCAACG 4185 0.0 27.581062 3 TCAACGC 4250 0.0 27.159233 4 CAACGCA 4285 0.0 26.826544 5 AACGCAG 4405 0.0 26.09574 6 GTGTAAG 265 3.092282E-11 23.3094 1 ACGCAGA 4915 0.0 23.194653 7 GAGTACT 2970 0.0 22.95421 12-13 CGCAGAG 4975 0.0 22.824116 8 GCAGAGT 5020 0.0 21.86238 9 GGCCTAA 220 5.6215868E-8 21.591234 6 AGTACTT 3125 0.0 21.435616 12-13 GTACTTT 3220 0.0 21.316315 14-15 GGTTAAC 160 4.9811657E-5 20.781563 2 AGAGTAC 4645 0.0 20.558882 10-11 CTAAGGG 140 4.8457456E-4 20.35745 2 CAGAGTA 4895 0.0 19.605947 10-11 >>END_MODULE