FastQCFastQC Report
Thu 26 May 2016
SRR936783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722802
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131501.8193087456869241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90581.2531786021621412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63160.8738215998295521No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.2699217766414592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.22841663415430505No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.20088488963782614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.13876552638205208No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9770.13516841403316537No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.11538429611428855No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.10902017426625825No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGC250.005003994357.04752
GGTATCA39600.052.578021
GATCCGC405.3665956E-447.5362856
GTATCCG405.3665956E-447.5362851
GTATCAA78850.046.7224121
TATCAAC101450.036.2228662
ATCAACG103250.035.588913
TCAACGC105100.035.0076944
CAACGCA106750.034.4220625
AACGCAG108750.033.7890136
CGATCGT452.4133646E-531.66671424-25
CATTCCG1551.3424142E-930.67079
ACGCAGA119400.030.496487
CGCAGAG119650.030.275948
GCAGAGT122300.028.8424959
GAGTACT74700.025.22478312-13
AGTACTT75150.024.7891712-13
AGAGTAC113800.023.8291510-11
CGCTCCG500.00165784123.74016848-49
GTGGTAT17350.023.5626561
Produced by FastQC (version 0.11.5)