##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 722802 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0713943790969 33.0 31.0 34.0 31.0 34.0 2 32.12776666362296 33.0 31.0 34.0 30.0 34.0 3 32.09771832396701 34.0 31.0 34.0 30.0 34.0 4 35.70759765468275 37.0 35.0 37.0 35.0 37.0 5 35.75073118226015 37.0 35.0 37.0 35.0 37.0 6 35.850941198281134 37.0 35.0 37.0 35.0 37.0 7 35.81489121502154 37.0 35.0 37.0 35.0 37.0 8 35.78801386825161 37.0 35.0 37.0 35.0 37.0 9 37.43509010766434 39.0 37.0 39.0 35.0 39.0 10-11 37.340084836511245 39.0 37.0 39.0 35.0 39.0 12-13 37.23947083710338 39.0 37.0 39.0 34.0 39.0 14-15 38.342756384182664 40.0 38.0 41.0 34.0 41.0 16-17 38.31973278989267 40.0 38.0 41.0 34.0 41.0 18-19 38.410114388172694 40.0 38.0 41.0 34.0 41.0 20-21 38.43354473285906 40.0 38.0 41.0 34.0 41.0 22-23 38.310463999822915 40.0 38.0 41.0 34.0 41.0 24-25 38.160781929214366 40.0 38.0 41.0 34.0 41.0 26-27 38.0714656295915 40.0 38.0 41.0 34.0 41.0 28-29 38.07951555197689 40.0 38.0 41.0 34.0 41.0 30-31 37.801595734378154 40.0 37.0 41.0 33.5 41.0 32-33 37.45628194166591 40.0 36.0 41.0 33.0 41.0 34-35 37.205264373922596 39.5 35.0 41.0 33.0 41.0 36-37 36.99951093107103 39.0 35.0 41.0 32.5 41.0 38-39 36.79061900769506 39.0 35.0 41.0 31.5 41.0 40-41 36.51514868525544 39.0 35.0 41.0 31.0 41.0 42-43 36.204976604934686 39.0 35.0 41.0 30.5 41.0 44-45 35.74085226659583 39.0 35.0 41.0 29.5 41.0 46-47 35.08094332887845 39.0 35.0 40.0 25.0 41.0 48-49 34.46574373064823 38.5 35.0 40.0 21.5 41.0 50-51 33.71646176961326 37.5 33.5 39.5 18.5 40.5 52-53 33.946145002365796 38.5 34.5 40.0 18.0 40.5 54-55 34.21311092110979 39.0 35.0 40.5 16.0 41.0 56-57 34.077527455651754 39.0 35.0 41.0 12.5 41.0 58-59 33.847195359171664 38.0 34.0 40.5 8.5 41.0 60-61 33.55789967376958 38.0 34.0 40.0 7.0 41.0 62-63 33.28630731514301 38.0 34.0 40.0 3.5 41.0 64-65 32.97573471019726 37.0 33.5 40.0 2.0 41.0 66-67 32.592061173046005 36.5 33.0 40.0 2.0 41.0 68-69 32.16203538451748 36.0 33.0 39.0 2.0 41.0 70-71 31.80305048962233 35.5 32.5 39.0 2.0 41.0 72-73 31.428699699226065 35.0 32.0 38.5 2.0 40.0 74-75 31.003053394982306 35.0 31.5 37.5 2.0 40.0 76-77 30.58589420062479 35.0 31.0 37.0 2.0 39.0 78-79 30.151927498817102 35.0 31.0 36.5 2.0 39.0 80-81 29.73100309628363 35.0 30.5 36.0 2.0 38.5 82-83 29.318915830338046 35.0 30.0 35.5 2.0 37.0 84-85 28.967199177644776 34.0 30.0 35.0 2.0 37.0 86-87 28.633923951510926 34.0 29.0 35.0 2.0 36.5 88-89 28.26173488728587 34.0 29.0 35.0 2.0 36.0 90-91 27.922202069169703 34.0 29.0 35.0 2.0 36.0 92-93 27.564263795617613 34.0 27.5 35.0 2.0 35.0 94-95 27.184927545856265 34.0 27.0 35.0 2.0 35.0 96-97 26.762034416064154 34.0 25.5 35.0 2.0 35.0 98-99 26.235971261839342 34.0 24.5 35.0 2.0 35.0 100-101 24.377509331739535 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5480864836887243 1101 2 0.4751661906020672 1101 3 0.6361979141476475 1101 4 0.46619215456939855 1101 5 0.3674457241913487 1101 6 0.3897472313293022 1101 7 0.45311105390518946 1101 8 0.45914062813086076 1101 9 0.43351769874413293 1101 10-11 0.4758054113496186 1101 12-13 0.6765588226939627 1101 14-15 0.8227755258045804 1101 16-17 0.8896388037303851 1101 18-19 0.8045223262060617 1101 20-21 0.7114323320818201 1101 22-23 0.8503226248945523 1101 24-25 0.7888463631853995 1101 26-27 0.9401967857181859 1101 28-29 0.8471483283237404 1101 30-31 0.8139757733248487 1101 32-33 0.8503672181749806 1101 34-35 0.9155231254598633 1101 36-37 0.818430902313338 1101 38-39 0.9467726037235167 1101 40-41 0.8904859553187023 1101 42-43 1.0743548472643027 1101 44-45 1.2196786627657303 1101 46-47 1.0682276267462285 1101 48-49 1.0123218458048981 1101 50-51 1.082945747729866 1101 52-53 1.1829364823128685 1101 54-55 1.1338327954272032 1101 56-57 1.1015586279016674 1101 58-59 0.9896494700822416 1101 60-61 1.1168606936878476 1101 62-63 1.150919219195849 1101 64-65 1.1387082747330695 1101 66-67 1.0278986715434684 1101 68-69 1.0324990221954522 1101 70-71 1.1346112953470922 1101 72-73 1.2091662621369643 1101 74-75 1.1248623162211793 1101 76-77 1.2282979467832256 1101 78-79 1.339508775908346 1101 80-81 1.2964054446657371 1101 82-83 1.2026912328902277 1101 84-85 1.3013533880153894 1101 86-87 1.428963647615582 1101 88-89 1.6598989287952897 1101 90-91 1.762729297832358 1101 92-93 1.615851619865726 1101 94-95 1.7645222546067565 1101 96-97 1.7370513557432687 1101 98-99 1.6290237384254098 1101 100-101 1.6352991140760338 1102 1 0.5053333254811712 1102 2 0.49725140258148315 1102 3 0.5009193066739535 1102 4 0.31981675849212365 1102 5 0.37035407654774843 1102 6 0.3539431708037739 1102 7 0.4055585937779185 1102 8 0.35601242212904083 1102 9 0.3637697685673018 1102 10-11 0.5349950665873067 1102 12-13 0.5039429523460939 1102 14-15 0.6559874806186414 1102 16-17 0.5089872495174887 1102 18-19 0.537571685857472 1102 20-21 0.6397037789326987 1102 22-23 0.5272917961702959 1102 24-25 0.4885542502811333 1102 26-27 0.5885617957691736 1102 28-29 0.662382775784117 1102 30-31 0.6076774821829716 1102 32-33 0.6865012484528918 1102 34-35 0.6862346070633265 1102 36-37 0.6655718111805555 1102 38-39 0.5310601662346386 1102 40-41 0.6292121859501947 1102 42-43 0.5946614971982456 1102 44-45 0.7372205797608018 1102 46-47 0.703157714611983 1102 48-49 0.7799494139188354 1102 50-51 0.6251894947249781 1102 52-53 0.7211073056614552 1102 54-55 0.7374500645856017 1102 56-57 0.9677850667458259 1102 58-59 0.9671601362491984 1102 60-61 0.8729568292607937 1102 62-63 0.9233094154078927 1102 64-65 1.0391348267692067 1102 66-67 0.9718939602587184 1102 68-69 1.0511551135516015 1102 70-71 1.0099882075911353 1102 72-73 0.9366111520303271 1102 74-75 0.9952683877024775 1102 76-77 1.147801984216077 1102 78-79 1.0765584213789658 1102 80-81 1.2394604639159432 1102 82-83 1.0719618355333935 1102 84-85 0.9919990554884102 1102 86-87 1.1410994647367225 1102 88-89 1.0901228425876788 1102 90-91 1.120233755476228 1102 92-93 1.1566083025458376 1102 94-95 1.2675437042969016 1102 96-97 1.1248155019009864 1102 98-99 1.1100756761281758 1102 100-101 1.421348297693104 1103 1 0.3089784460283447 1103 2 0.37397582141347385 1103 3 0.39550065778290744 1103 4 0.2944846292340273 1103 5 0.3387588940900059 1103 6 0.2457917676526975 1103 7 0.17601344250820716 1103 8 0.16989776809895574 1103 9 0.3732099666811237 1103 10-11 0.43804559740449633 1103 12-13 0.30173465353683326 1103 14-15 0.34666520739907725 1103 16-17 0.4687649300589456 1103 18-19 0.5608019590398072 1103 20-21 0.41573119957390503 1103 22-23 0.4218967322581051 1103 24-25 0.6306202323627588 1103 26-27 0.6849653968230527 1103 28-29 0.6920548514794831 1103 30-31 0.8111447844027495 1103 32-33 0.7711902525771563 1103 34-35 0.798332149570598 1103 36-37 0.8287804854875134 1103 38-39 0.7722649862595219 1103 40-41 0.7962754526559692 1103 42-43 0.8337719090354199 1103 44-45 0.9711751910896638 1103 46-47 0.8402792300089388 1103 48-49 0.893998923754701 1103 50-51 1.0080875920999048 1103 52-53 1.1742306085515466 1103 54-55 1.1233096284496895 1103 56-57 1.0347111681798538 1103 58-59 1.2035592065045009 1103 60-61 1.0873309428845275 1103 62-63 1.126078478122217 1103 64-65 1.0533730164358772 1103 66-67 1.1463359117295226 1103 68-69 1.3201793471173744 1103 70-71 1.2463152815952085 1103 72-73 1.122896797157079 1103 74-75 1.0152343136146094 1103 76-77 1.1414824511986588 1103 78-79 1.2065609971069193 1103 80-81 1.1729303418636796 1103 82-83 1.073110361937708 1103 84-85 1.206992895464328 1103 86-87 1.2495664721442665 1103 88-89 1.1510807030577013 1103 90-91 1.1309140269517428 1103 92-93 1.2450541455381519 1103 94-95 1.1118915330823675 1103 96-97 1.281664921120882 1103 98-99 1.4291807503122484 1103 100-101 1.5971252830983467 1104 1 0.4023322558982194 1104 2 0.30556042196103306 1104 3 0.3596073003165152 1104 4 0.36844697617419797 1104 5 0.2210398339995976 1104 6 0.22196402651633917 1104 7 0.11512408436535537 1104 8 0.1750680935753337 1104 9 0.23252062042197252 1104 10-11 0.2616900552562029 1104 12-13 0.24500916253415994 1104 14-15 0.2624444813047475 1104 16-17 0.2648890142138569 1104 18-19 0.2902369803162088 1104 20-21 0.09095459036037568 1104 22-23 0.2621715200654222 1104 24-25 0.29892273662489544 1104 26-27 0.39552863869757005 1104 28-29 0.44400766403233405 1104 30-31 0.5156436805894558 1104 32-33 0.44019800897950745 1104 34-35 0.5261217348750264 1104 36-37 0.4442027912003681 1104 38-39 0.469901043246459 1104 40-41 0.6214894735987073 1104 42-43 0.7011150327127851 1104 44-45 0.7198947219575871 1104 46-47 0.8860991370418034 1104 48-49 0.6965415793969427 1104 50-51 0.8431984533485206 1104 52-53 1.0748828410882183 1104 54-55 1.1193697234279156 1104 56-57 1.0143073050618412 1104 58-59 1.0923117375049785 1104 60-61 1.1012504170763222 1104 62-63 1.2720569869001537 1104 64-65 1.333737937569218 1104 66-67 1.3492661589562829 1104 68-69 1.1366524465245575 1104 70-71 1.325703453652963 1104 72-73 1.481926355586058 1104 74-75 1.200970211999799 1104 76-77 1.1589067040107537 1104 78-79 1.313586551641695 1104 80-81 1.3013876999334002 1104 82-83 1.369824517714786 1104 84-85 1.3456336739383694 1104 86-87 1.2109700290283598 1104 88-89 1.248392243505716 1104 90-91 1.3338246203599482 1104 92-93 1.1870452144773473 1104 94-95 1.254414169253618 1104 96-97 1.3176222677639657 1104 98-99 1.3347793462068864 1104 100-101 1.5683639764393575 1105 1 0.261484526925404 1105 2 0.3705930582914121 1105 3 0.3385562263819999 1105 4 0.24835902936175103 1105 5 0.3518391619312169 1105 6 0.2559100047090723 1105 7 0.36484619201281276 1105 8 0.2656481836660518 1105 9 0.3284977645042062 1105 10-11 0.3456356838987986 1105 12-13 0.31959558647321984 1105 14-15 0.42332958883802263 1105 16-17 0.6009016492858521 1105 18-19 0.3176149115853235 1105 20-21 0.5258240799938818 1105 22-23 0.5017147279568661 1105 24-25 0.5840198999090802 1105 26-27 0.6510579391545477 1105 28-29 0.5447421853700334 1105 30-31 0.5960087507441685 1105 32-33 0.7334332818499902 1105 34-35 0.5197138984272058 1105 36-37 0.5509018029056421 1105 38-39 0.6288694509058033 1105 40-41 0.6403255648825592 1105 42-43 0.6356891524873021 1105 44-45 0.7659770806286446 1105 46-47 0.7503373624457055 1105 48-49 0.725731345501309 1105 50-51 0.48010325686638566 1105 52-53 0.624555079583935 1105 54-55 0.7061624378710931 1105 56-57 0.756165699974261 1105 58-59 0.8949947677783499 1105 60-61 0.8916030665497843 1105 62-63 1.044048350681635 1105 64-65 1.114215795898879 1105 66-67 1.2374695412033248 1105 68-69 1.3043887365859206 1105 70-71 1.2187568439396657 1105 72-73 1.135741811760628 1105 74-75 1.268390275711134 1105 76-77 1.0511409818285387 1105 78-79 1.3739655105881958 1105 80-81 1.1657896313143787 1105 82-83 1.142731702834208 1105 84-85 1.3721528770572853 1105 86-87 1.1778029650397137 1105 88-89 1.0586675025806223 1105 90-91 1.1264446850034169 1105 92-93 0.8199942757022178 1105 94-95 1.2633579918872258 1105 96-97 1.2252017962441428 1105 98-99 1.3089982764436705 1105 100-101 1.3730120610211678 1106 1 0.1575113906662402 1106 2 0.08536765700969795 1106 3 0.23145678558453042 1106 4 0.06536126341943316 1106 5 0.029708228718995144 1106 6 0.0510877430353176 1106 7 0.1520762816044865 1106 8 0.11670947574959456 1106 9 0.08594424116128607 1106 10-11 0.2872290670511646 1106 12-13 0.2701630462991744 1106 14-15 0.3194367318589926 1106 16-17 0.28777231707184114 1106 18-19 0.23550526385081128 1106 20-21 0.2675977474725144 1106 22-23 0.530970791336209 1106 24-25 0.5636530465357481 1106 26-27 0.4706520471141218 1106 28-29 0.4573799790201747 1106 30-31 0.5545617052510465 1106 32-33 0.36094200935458787 1106 34-35 0.6919645624515312 1106 36-37 0.5732240301739893 1106 38-39 0.8298818873131424 1106 40-41 0.7043723745839614 1106 42-43 0.7249929705698648 1106 44-45 0.9785199153588309 1106 46-47 0.8679143961492457 1106 48-49 1.2193846949822387 1106 50-51 0.8967848325422239 1106 52-53 0.9251551098606967 1106 54-55 1.088659949213401 1106 56-57 1.0771635484895512 1106 58-59 1.4519643855113529 1106 60-61 1.3815131078821494 1106 62-63 1.3774447605922333 1106 64-65 0.9446246697411382 1106 66-67 1.1246427471907836 1106 68-69 1.066016616891801 1106 70-71 1.1313109177690777 1106 72-73 0.9114311814077176 1106 74-75 0.9160926827133231 1106 76-77 0.9710646935754603 1106 78-79 1.0283540347797846 1106 80-81 1.1884547657936402 1106 82-83 1.0558179136063082 1106 84-85 1.2108924464851007 1106 86-87 1.1475411169648133 1106 88-89 1.104099840909619 1106 90-91 0.8731612624454002 1106 92-93 1.0060082488843527 1106 94-95 1.0987487684860433 1106 96-97 0.6471646017068053 1106 98-99 0.8945679611525321 1106 100-101 1.3337004443799607 1107 1 0.41733408323278454 1107 2 0.5122476805269116 1107 3 0.5221496925135973 1107 4 0.5083088061024057 1107 5 0.38728208747645 1107 6 0.3943307981073758 1107 7 0.32616943763039075 1107 8 0.4442718079766905 1107 9 0.5125999228124414 1107 10-11 0.545073296436847 1107 12-13 0.2757309793369913 1107 14-15 0.47362148125365877 1107 16-17 0.5001563748746847 1107 18-19 0.6308865289842132 1107 20-21 0.5316094441932577 1107 22-23 0.5634017288531368 1107 24-25 0.6073571628221899 1107 26-27 0.6490231704479967 1107 28-29 0.6072790251804534 1107 30-31 0.5272384131502008 1107 32-33 0.6607710453807982 1107 34-35 0.8721265527506787 1107 36-37 0.7091171815583337 1107 38-39 0.7153592326526379 1107 40-41 0.6891519393607481 1107 42-43 0.9128638115424224 1107 44-45 1.0978543162500145 1107 46-47 1.254457365352522 1107 48-49 0.7663379003633537 1107 50-51 0.6262614197609295 1107 52-53 0.5258768717952975 1107 54-55 0.6846782678871577 1107 56-57 0.68004556787578 1107 58-59 0.8402053905629074 1107 60-61 0.8564003567546408 1107 62-63 0.6634032805547889 1107 64-65 0.6556297986603496 1107 66-67 0.6609416479156422 1107 68-69 0.7838815785602833 1107 70-71 0.9130146782029129 1107 72-73 0.8895771892828925 1107 74-75 1.0839893616801142 1107 76-77 0.8345410641882225 1107 78-79 0.9970912221516599 1107 80-81 0.8092589927038354 1107 82-83 0.8906404127746974 1107 84-85 0.590482751822119 1107 86-87 0.6479859327337216 1107 88-89 0.7185664101036124 1107 90-91 0.44153911603525486 1107 92-93 0.5604865766864968 1107 94-95 0.7892730322192456 1107 96-97 1.221717658558127 1107 98-99 1.1856747401448473 1107 100-101 1.5144890447187933 1108 1 -0.03933196523012583 1108 2 -0.01470173974718847 1108 3 0.031764892651739984 1108 4 0.1387583255022804 1108 5 0.15437169078149537 1108 6 -0.1534510228650774 1108 7 -0.08306972408531976 1108 8 -0.20794381296634867 1108 9 -0.2021576667045295 1108 10-11 -0.30282140001444446 1108 12-13 -0.2995134776136368 1108 14-15 -0.04259997212205491 1108 16-17 -0.031552681383992365 1108 18-19 0.11744539370631202 1108 20-21 0.013616517296917152 1108 22-23 0.14422920523882254 1108 24-25 0.27285883516994147 1108 26-27 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-1.1299480289369015 2316 48-49 -1.0572296759717474 2316 50-51 -1.0641447739399439 2316 52-53 -1.176895579791072 2316 54-55 -1.6375395775252102 2316 56-57 -1.1566384386817106 2316 58-59 -1.2650200376725351 2316 60-61 -1.2572449594473483 2316 62-63 -0.8605500986964287 2316 64-65 -0.9306110558989005 2316 66-67 -1.1006237503129128 2316 68-69 -0.756571247475037 2316 70-71 -1.2214399551974253 2316 72-73 -1.0620062825957852 2316 74-75 -1.0862621053557504 2316 76-77 -1.1839728415159811 2316 78-79 -1.3932402439573508 2316 80-81 -1.4538313697539458 2316 82-83 -1.4791221388539562 2316 84-85 -1.0176289572384576 2316 86-87 -0.8369758635090427 2316 88-89 -1.6170922643795187 2316 90-91 -1.5130305630995267 2316 92-93 -1.8023636726951153 2316 94-95 -1.7141418449436507 2316 96-97 -1.7881634724352864 2316 98-99 -1.9431984952123074 2316 100-101 -1.8487536693410007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1896.0 3 483.0 4 455.0 5 403.0 6 501.0 7 546.0 8 768.0 9 857.0 10 1184.0 11 1720.0 12 2850.0 13 4059.0 14 4582.0 15 6552.0 16 9399.0 17 14365.0 18 12272.0 19 6234.0 20 5105.0 21 4702.0 22 4900.0 23 5010.0 24 5272.0 25 5919.0 26 6539.0 27 7381.0 28 8788.0 29 10872.0 30 12917.0 31 16491.0 32 21697.0 33 29108.0 34 41298.0 35 64337.0 36 93611.0 37 139743.0 38 139897.0 39 30072.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.628132354977154 19.41492454658729 15.74262771701509 19.214315381420462 2 15.002852964313018 21.431578714116355 42.18744806497003 21.378120256600596 3 18.155019460856057 28.959498434685322 31.790634142829255 21.094847961629366 4 11.417459747867374 18.063685725092586 39.40753405203647 31.111320475003566 5 11.48462022212926 39.01972789304108 33.80226672687631 15.693385157953351 6 26.778145310084607 38.814181945113944 18.637904955037314 15.769767789764133 7 24.685594921313886 37.12771100358905 21.49146926455738 16.695224810539685 8 26.17697857470322 33.29999986149527 20.284571819550496 20.238449744251014 9 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26.182125753331547 35.50166845590479 19.414514216656322 34-35 19.207383542065358 26.207560032806516 35.44107112576869 19.143985299359443 36-37 19.394827021747993 25.997645311352546 35.27914688564394 19.328380781255518 38-39 19.176018089838724 26.33387989526498 35.082285371774006 19.407816643122285 40-41 19.217164375406682 26.437748335202336 34.982902078055126 19.362185211335852 42-43 19.10800647046242 26.58551593770138 34.536404657237846 19.770072934598353 44-45 19.30364262633407 26.765652607516405 34.16862813763263 19.76207662851689 46-47 19.67311355909567 27.054389037630212 33.46008446538587 19.812412937888254 48-49 19.64757672666573 27.51813709240419 32.670988333989634 20.16329784694045 50-51 19.701248189713212 27.936435667268423 32.053199078613325 20.30911706440504 52-53 19.86032094902033 28.327152364627096 31.546330968210285 20.266195718142292 54-55 19.553187071769674 28.192954529725238 31.606547165893833 20.647311232611255 56-57 19.75226442661403 28.444949534448977 31.21458535055961 20.588200688377388 58-59 19.803897682376817 27.92526904196972 31.564845551774834 20.705987723878636 60-61 19.883598279098354 27.918140894669524 31.92631074788348 20.271950078348645 62-63 19.83508485592247 27.803145611364787 32.03026420615985 20.33150532655289 64-65 19.61201437783477 28.000445674123636 31.926045777099272 20.461494170942323 66-67 19.753843597926085 28.09346467212604 31.58783339448017 20.564858335467704 68-69 19.74310595668045 28.009797909465544 31.651275818488124 20.595820315365884 70-71 19.784721986768623 28.185075985970116 31.40898740036853 20.62121462689273 72-73 19.803612658071952 28.198991632919512 31.199046292586974 20.798349416421562 74-75 19.5857590576714 28.65702812467779 30.92579688201724 20.831415935633565 76-77 19.6339501067559 28.40814320862906 30.932565567501914 21.02534111711313 78-79 19.626563513484513 28.414012901993168 30.84232612456328 21.117097459959034 80-81 19.52577135513375 28.59856729300595 30.774618206631754 21.101043145228545 82-83 19.520069777825466 28.600878452981306 30.62658133650841 21.25247043268482 84-85 19.544555250504303 28.48274152928977 30.365452322704535 21.607250897501395 86-87 19.5506392688058 28.347205098366885 30.470578543634275 21.631577089193037 88-89 19.627646241772364 28.23874235053157 30.395411219081886 21.738200188614183 90-91 19.43193382022083 28.392717455262883 30.436260427122637 21.739088297393653 92-93 19.592395970069067 28.546821084580916 30.132055563863545 21.728727381486465 94-95 19.50299859728513 28.539985682061452 30.004943427526165 21.95207229312725 96-97 19.531152079540583 28.54012864822418 29.94073096400301 21.987988308232225 98-99 19.67587974508174 28.80874203380438 29.458160155167633 22.057218065946245 100-101 19.8945905808947 28.7547862996847 29.37589699827666 21.97472612114395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1217.0 1 899.0 2 745.5 3 877.5 4 712.0 5 677.0 6 1216.0 7 2775.0 8 4993.5 9 5715.0 10 4311.5 11 3058.5 12 2842.5 13 2961.5 14 3046.5 15 3059.5 16 3118.5 17 3229.0 18 3306.0 19 3372.5 20 3645.0 21 4023.0 22 4469.5 23 5086.5 24 5585.0 25 6397.0 26 7762.0 27 9006.0 28 9869.0 29 10928.0 30 12279.5 31 13638.0 32 15124.0 33 16382.5 34 17684.0 35 18654.5 36 19880.0 37 21396.5 38 21711.5 39 21084.0 40 20981.0 41 21632.0 42 21785.0 43 22223.0 44 23263.5 45 23740.0 46 23409.5 47 23340.0 48 24618.0 49 24116.5 50 21602.5 51 20102.0 52 18834.0 53 17753.5 54 16863.0 55 15618.0 56 14105.0 57 12493.0 58 11341.0 59 10242.0 60 8945.0 61 7528.0 62 6120.0 63 4585.0 64 3129.5 65 2272.0 66 1696.5 67 1313.0 68 925.5 69 565.5 70 364.0 71 278.0 72 226.5 73 162.5 74 119.0 75 116.5 76 101.5 77 58.0 78 28.0 79 14.0 80 13.0 81 13.5 82 7.5 83 3.5 84 2.5 85 2.5 86 2.5 87 1.5 88 2.0 89 3.0 90 2.0 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06945193842850463 2 0.10320945431805668 3 0.08065832690003626 4 0.14374614348051057 5 0.12174841796231888 6 0.12036491321274706 7 0.1221634693871904 8 0.11137213234053032 9 0.13392325975855074 10-11 0.11953481036300398 12-13 0.1031402790805781 14-15 0.10929687521617261 16-17 0.1202265627377899 18-19 0.09732955913237651 20-21 0.08017410023768612 22-23 0.08363286211161564 24-25 0.06979781461589758 26-27 0.06350286800534585 28-29 0.07643863741384224 30-31 0.05623946807009389 32-33 0.05914482804419467 34-35 0.05319575762103591 36-37 0.05720792139479415 38-39 0.05637781854505106 40-41 0.06806843367893281 42-43 0.06122008516855238 44-45 0.04738503767283433 46-47 0.03562524730147398 48-49 0.044064626273862 50-51 0.027047517854128792 52-53 0.03991411202514658 54-55 0.048699367184927546 56-57 0.05236565477129283 58-59 0.05866060138184455 60-61 0.05395668523330041 62-63 0.05955987946906621 64-65 0.04164349296211134 66-67 0.06876018605371872 68-69 0.05575524140774375 70-71 0.027877620703871876 72-73 0.02054504553114131 74-75 0.0347951444517309 76-77 0.049736995747106404 78-79 0.07062791746564066 80-81 0.0447563786486479 82-83 0.07048956699068347 84-85 0.0718038965027767 86-87 0.07353327743974145 88-89 0.09587687914532611 90-91 0.07291070030243414 92-93 0.0838403878240514 94-95 0.08729914969798092 96-97 0.10452378383014989 98-99 0.1386271759070949 100-101 0.1314329512093215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 722802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.24904948300944 #Duplication Level Percentage of deduplicated Percentage of total 1 80.29552463369166 48.377290369194924 2 11.703793151914518 14.102848254972095 3 3.4708473491714895 6.273457610646156 4 1.534360738411712 3.6977510421341653 5 0.8136266866735841 2.4510117253046895 6 0.48339057830746834 1.7474293723240324 7 0.30937182607978875 1.3047550920691127 8 0.23496248122082752 1.1325012926179452 9 0.1814829066663173 0.9840755361653402 >10 0.890632871955898 9.709558083682856 >50 0.05360239893823392 2.1430544336302546 >100 0.02542649873857647 2.705016095364558 >500 0.0013744053369052348 0.5986918275555809 >1k 9.162702246034898E-4 0.8359327799686846 >5k 4.581351123017449E-4 2.1217815022682114 >10k+ 2.2906755615087246E-4 1.8148449821014039 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13150 1.8193087456869241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9058 1.2531786021621412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6316 0.8738215998295521 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1951 0.2699217766414592 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.22841663415430505 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1452 0.20088488963782614 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.13876552638205208 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 977 0.13516841403316537 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.11538429611428855 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.10902017426625825 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.917523747859026E-5 0.0 20-21 0.0 0.0 0.0 3.458761873929513E-4 0.0 22-23 0.0 0.0 0.0 7.609276122644929E-4 0.0 24-25 0.0 0.0 0.0 0.001314329512093215 0.0 26-27 0.0 0.0 0.0 0.0020752571243577078 0.0 28-29 0.0 0.0 0.0 0.00532649328585145 0.0 30-31 0.0 0.0 0.0 0.013627521783282283 0.0 32-33 0.0 0.0 0.0 0.027255043566564566 0.0 34-35 0.0 0.0 0.0 0.04427215198629777 0.0 36-37 0.0 0.0 0.0 0.0696594641409404 0.0 38-39 0.0 0.0 0.0 0.10998862759095851 0.0 40-41 0.0 0.0 0.0 0.15260057387777012 0.0 42-43 0.0 0.0 0.0 0.20648808387359194 0.0 44-45 0.0 0.0 0.0 0.27614754801453234 0.0 46-47 0.0 0.0 0.0 0.36337752247503463 0.0 48-49 0.0 0.0 0.0 0.46243646254437587 0.0 50-51 0.0 0.0 0.0 0.5800343662579793 0.0 52-53 0.0 0.0 0.0 0.711951544129651 0.0 54-55 0.0 0.0 0.0 0.8336999620919698 0.0 56-57 0.0 0.0 0.0 1.0029717682020802 0.0 58-59 0.0 0.0 0.0 1.1970083093295258 0.0 60-61 0.0 0.0 0.0 1.4061942274647827 0.0 62-63 0.0 0.0 0.0 1.6603440499611235 0.0 64-65 0.0 0.0 0.0 1.907437998234648 0.0 66-67 0.0 0.0 0.0 2.1810952376999513 0.0 68-69 0.0 0.0 0.0 2.5091933890609046 0.0 70-71 0.0 0.0 0.0 2.8633014297138084 0.0 72-73 0.0 0.0 0.0 3.276485123173428 0.0 74-75 0.0 0.0 0.0 3.6839272719223244 0.0 76-77 0.0 0.0 0.0 4.100085500593524 0.0 78-79 0.0 0.0 0.0 4.578501442995454 0.0 80-81 0.0 0.0 0.0 5.1466376684071165 0.0 82-83 0.0 0.0 0.0 5.694090497812679 0.0 84-85 0.0 0.0 0.0 6.302140835249487 0.0 86-87 0.0 0.0 0.0 6.976876101615657 0.0 88-89 0.0 0.0 0.0 7.7010024875415395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCCGC 25 0.0050039943 57.0475 2 GGTATCA 3960 0.0 52.57802 1 GATCCGC 40 5.3665956E-4 47.536285 6 GTATCCG 40 5.3665956E-4 47.536285 1 GTATCAA 7885 0.0 46.722412 1 TATCAAC 10145 0.0 36.222866 2 ATCAACG 10325 0.0 35.58891 3 TCAACGC 10510 0.0 35.007694 4 CAACGCA 10675 0.0 34.422062 5 AACGCAG 10875 0.0 33.789013 6 CGATCGT 45 2.4133646E-5 31.666714 24-25 CATTCCG 155 1.3424142E-9 30.6707 9 ACGCAGA 11940 0.0 30.49648 7 CGCAGAG 11965 0.0 30.27594 8 GCAGAGT 12230 0.0 28.842495 9 GAGTACT 7470 0.0 25.224783 12-13 AGTACTT 7515 0.0 24.78917 12-13 AGAGTAC 11380 0.0 23.82915 10-11 CGCTCCG 50 0.001657841 23.740168 48-49 GTGGTAT 1735 0.0 23.562656 1 >>END_MODULE