FastQCFastQC Report
Thu 26 May 2016
SRR936783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722802
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111331.5402558376982909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83141.1502458487939988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64170.8877949978002274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.25608672914574115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.23547250837712125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.22993848937883404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.22122240945653165No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9450.13074119883453558No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.11787460466351782No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.10639151524207183No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.10431625811771411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.046.9899981
GTATCAA84800.037.1963731
CTACACG902.4126934E-531.6648244
TATCAAC110950.028.2971362
ATCAACG111350.028.1101723
TCAACGC112800.027.7909344
CAACGCA114200.027.3670565
AACGCAG116950.026.7660056
TGCGTAA458.951711E-426.38735260-61
ACGCAGA131650.023.5247547
CGCAGAG131950.023.3272728
GAGTACT77450.023.27414312-13
AGTACTT79300.022.82102212-13
GCAGAGT135400.022.4171589
GTACATG66300.022.3547331
TAATACC2554.8930815E-1022.351644
GTGTAAG2606.2391337E-1021.9248311
TTGCGTA652.9431758E-421.921860-61
GTAAGGT2606.2391337E-1021.92183
TACATGG67650.021.9055982
Produced by FastQC (version 0.11.5)