FastQCFastQC Report
Thu 26 May 2016
SRR936782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500525
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95671.9113930373108237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66791.3343988811747665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.9438090005494232No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.28550022476399783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.2339543479346686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.22995854352929426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7760.15503721092852504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.13685630088407172No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.13525797912192197No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.1198741321612307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.052.886161
GTATCAA58800.044.6114541
TATCAAC74500.035.0825422
ATCAACG75550.034.6578643
TCAACGC76450.034.2498554
CAACGCA77650.033.669465
AACGCAG79100.033.052266
TAGGACA1401.6945705E-830.5491494
CGCAGAG86300.029.9091438
ACGCAGA87000.029.8870497
GCAGAGT87800.028.9650429
GAGTACT54300.026.3447912-13
GTACATG43550.026.1883261
TACATGG43700.025.7722032
AGTACTT54650.025.04553812-13
ACATGGG44050.024.4886363
GTACTTT59100.024.08448814-15
GTATAGA1601.84897E-623.7604481
CAGAGTA85700.023.35040910-11
CATGGGA34300.023.2755414
Produced by FastQC (version 0.11.5)