##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936782_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 500525 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09043104740023 33.0 31.0 34.0 31.0 34.0 2 32.17142200689276 33.0 31.0 34.0 31.0 34.0 3 32.13756355826382 34.0 31.0 34.0 30.0 34.0 4 35.75420408571001 37.0 35.0 37.0 35.0 37.0 5 35.78068428150442 37.0 35.0 37.0 35.0 37.0 6 35.883750062434444 37.0 35.0 37.0 35.0 37.0 7 35.85001548374207 37.0 35.0 37.0 35.0 37.0 8 35.812093302032864 37.0 35.0 37.0 35.0 37.0 9 37.4740522451426 39.0 37.0 39.0 35.0 39.0 10-11 37.3366515159083 39.0 37.0 39.0 35.0 39.0 12-13 37.27431596823335 39.0 37.0 39.0 34.5 39.0 14-15 38.37797312821537 40.0 38.0 41.0 34.0 41.0 16-17 38.356870286199495 40.0 38.0 41.0 34.0 41.0 18-19 38.47989211328105 40.0 38.0 41.0 34.0 41.0 20-21 38.49698516557615 40.0 38.0 41.0 34.0 41.0 22-23 38.39747864742021 40.0 38.0 41.0 34.0 41.0 24-25 38.25272164227562 40.0 38.0 41.0 34.0 41.0 26-27 38.25418810249238 40.0 38.0 41.0 34.0 41.0 28-29 38.15682133759553 40.0 38.0 41.0 34.0 41.0 30-31 37.86502871984416 40.0 37.5 41.0 34.0 41.0 32-33 37.500412566804854 40.0 36.0 41.0 33.0 41.0 34-35 37.266719944058735 40.0 35.0 41.0 33.0 41.0 36-37 37.03599720293692 39.5 35.0 41.0 33.0 41.0 38-39 36.84092702662204 39.0 35.0 41.0 32.0 41.0 40-41 36.583485340392585 39.0 35.0 41.0 31.0 41.0 42-43 36.23504320463513 39.0 35.0 41.0 30.5 41.0 44-45 35.729590929524 39.0 35.0 41.0 29.5 41.0 46-47 35.03318115978223 39.0 35.0 40.5 25.0 41.0 48-49 34.39706208481095 39.0 35.0 40.0 20.5 41.0 50-51 33.597516607562056 38.0 34.0 39.5 14.5 40.5 52-53 33.74347335297937 38.5 34.5 40.0 12.0 40.5 54-55 34.074137155986215 39.0 35.0 40.5 8.5 41.0 56-57 33.95567154487787 39.0 35.0 41.0 7.0 41.0 58-59 33.71064182608261 38.5 34.5 41.0 3.5 41.0 60-61 33.43599320713251 38.0 34.0 40.0 2.0 41.0 62-63 33.147439188851706 38.0 34.0 40.0 2.0 41.0 64-65 32.8197752360022 37.0 34.0 40.0 2.0 41.0 66-67 32.43703711103342 37.0 33.0 40.0 2.0 41.0 68-69 32.047961640277705 36.0 33.0 39.0 2.0 41.0 70-71 31.63148793766545 36.0 32.5 39.0 2.0 41.0 72-73 31.23295040207782 35.0 32.0 39.0 2.0 40.0 74-75 30.812517856250935 35.0 31.5 37.5 2.0 40.0 76-77 30.380293691623795 35.0 31.0 37.0 2.0 39.0 78-79 29.94631237200939 35.0 31.0 36.5 2.0 39.0 80-81 29.5159862144748 35.0 30.0 36.0 2.0 38.5 82-83 29.112628739823187 35.0 30.0 36.0 2.0 37.0 84-85 28.76601568353229 34.5 29.5 35.0 2.0 37.0 86-87 28.422174716547627 34.0 29.0 35.0 2.0 36.5 88-89 28.060473502822035 34.0 29.0 35.0 2.0 36.0 90-91 27.68990060436542 34.0 28.5 35.0 2.0 36.0 92-93 27.325576145047698 34.0 27.0 35.0 2.0 35.5 94-95 26.92903950851606 34.0 26.0 35.0 2.0 35.0 96-97 26.483467359272765 34.0 25.0 35.0 2.0 35.0 98-99 25.94701163778033 34.0 23.5 35.0 2.0 35.0 100-101 24.069759752260126 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39051998168621793 1101 2 0.5156205821066351 1101 3 0.5114828477791633 1101 4 0.32417702767059353 1101 5 0.45412712385336107 1101 6 0.31611572863533155 1101 7 0.38132951815344285 1101 8 0.3294531029360499 1101 9 0.32595766490918265 1101 10-11 0.4384301088478324 1101 12-13 0.5228905493350524 1101 14-15 0.6892521535502354 1101 16-17 0.8465616683791524 1101 18-19 0.6787314177479828 1101 20-21 0.6594467136409108 1101 22-23 0.6793884655308133 1101 24-25 0.6728040779025051 1101 26-27 0.8292251998466114 1101 28-29 0.8640456390465943 1101 30-31 0.8376214143885718 1101 32-33 0.8706557356305495 1101 34-35 0.8805795694672369 1101 36-37 0.8791565601941542 1101 38-39 1.0108847845881357 1101 40-41 1.0223361050900763 1101 42-43 1.0371511655297923 1101 44-45 1.3755938940473769 1101 46-47 1.7472645617557916 1101 48-49 2.0376257688197583 1101 50-51 1.9950682816573178 1101 52-53 2.0224478522344 1101 54-55 2.0827777127501506 1101 56-57 2.150952497675597 1101 58-59 2.1204106099760835 1101 60-61 2.1057420392314086 1101 62-63 2.0729304344172093 1101 64-65 2.1588889959187583 1101 66-67 2.154505939393726 1101 68-69 2.2400583813295007 1101 70-71 2.0675656592526543 1101 72-73 2.1202317411541394 1101 74-75 1.9569318890189074 1101 76-77 1.9530490678612509 1101 78-79 2.0135343403258013 1101 80-81 1.9477301388655128 1101 82-83 2.1168864261865963 1101 84-85 2.0833748334870315 1101 86-87 2.009637107691937 1101 88-89 1.8362873578534895 1101 90-91 1.9275632126539293 1101 92-93 2.0684475878397883 1101 94-95 2.1293647498429316 1101 96-97 2.0296558174833237 1101 98-99 2.245965622523279 1101 100-101 2.051937370832288 1102 1 0.2544895832673859 1102 2 0.12133307267421856 1102 3 0.08039550757766278 1102 4 0.18267977609431796 1102 5 0.01605427247030633 1102 6 0.010679185492499244 1102 7 -0.041226815850293974 1102 8 0.16324845350609962 1102 9 0.204528168844746 1102 10-11 0.3240050677240873 1102 12-13 0.2977360787657588 1102 14-15 0.3011576747088327 1102 16-17 0.2475923667408324 1102 18-19 0.20222404704703223 1102 20-21 0.1727507578177594 1102 22-23 0.21307915183163573 1102 24-25 0.14037236226096184 1102 26-27 0.2696747036779854 1102 28-29 0.23695274832366664 1102 30-31 0.332249280026808 1102 32-33 0.3145514249781556 1102 34-35 0.20111270999519348 1102 36-37 0.11178197577287818 1102 38-39 0.5437672038324735 1102 40-41 0.5368714936107892 1102 42-43 0.723559334078729 1102 44-45 0.8994740810854225 1102 46-47 1.0413869447544002 1102 48-49 1.3000931303014127 1102 50-51 1.2885378942890142 1102 52-53 1.3219999901954793 1102 54-55 1.4679445914816753 1102 56-57 1.5756865746567215 1102 58-59 1.6139342123213183 1102 60-61 1.4967956911145777 1102 62-63 1.4945380018175243 1102 64-65 1.6135810493275216 1102 66-67 1.195103204546367 1102 68-69 1.1763094745422684 1102 70-71 1.4086417279174732 1102 72-73 1.5080673219540053 1102 74-75 1.6767868429697863 1102 76-77 1.7341894505883282 1102 78-79 1.6676947055614768 1102 80-81 1.519983214858403 1102 82-83 1.432556545879578 1102 84-85 1.5063431942519792 1102 86-87 1.4772535407715317 1102 88-89 1.6310727724218772 1102 90-91 1.8061021436576041 1102 92-93 1.7786033371128198 1102 94-95 1.6402874056316712 1102 96-97 1.728972579237137 1102 98-99 2.0139448285903327 1102 100-101 2.162089015624179 1103 1 0.25618660971859697 1103 2 0.3074649501119424 1103 3 0.2952032115176806 1103 4 0.3880110154949037 1103 5 0.2957085495370393 1103 6 0.10376696712608435 1103 7 0.19723060390779779 1103 8 0.15631584841927548 1103 9 0.25073769504392374 1103 10-11 0.42274344337293 1103 12-13 0.2504181646633086 1103 14-15 0.4769662546057347 1103 16-17 0.6222896463458198 1103 18-19 0.4853759908424138 1103 20-21 0.545463132557046 1103 22-23 0.5564150465734272 1103 24-25 0.4153136104211157 1103 26-27 0.5552892960892066 1103 28-29 0.70257872145352 1103 30-31 0.6345669813008712 1103 32-33 0.652165596213166 1103 34-35 0.7290262747812832 1103 36-37 0.7040867729250024 1103 38-39 0.6335124594393804 1103 40-41 0.6930595353430817 1103 42-43 0.6703824781590697 1103 44-45 0.7930150751956759 1103 46-47 0.6204000482629297 1103 48-49 0.9413512597862095 1103 50-51 1.0649095733258704 1103 52-53 1.112183672306017 1103 54-55 1.2700315419643502 1103 56-57 1.3449380235700588 1103 58-59 1.4004309224423181 1103 60-61 1.4956240147660864 1103 62-63 1.3299337170217669 1103 64-65 1.3854726905481272 1103 66-67 1.4318423150132666 1103 68-69 1.2665195921803623 1103 70-71 1.2993011375090795 1103 72-73 1.4598280849102174 1103 74-75 1.058858626628595 1103 76-77 1.1796740160487431 1103 78-79 1.2492046222447613 1103 80-81 1.1123328179894436 1103 82-83 1.2329621520036618 1103 84-85 1.190709716120658 1103 86-87 1.3349469513802426 1103 88-89 1.3462658077805152 1103 90-91 1.322565540533283 1103 92-93 1.3150490804328072 1103 94-95 1.534163324434278 1103 96-97 1.6279227751512764 1103 98-99 1.8431231541460953 1103 100-101 1.906913925060092 1104 1 0.2688612078477419 1104 2 0.3049383857672012 1104 3 0.09067137971312178 1104 4 0.199891861921202 1104 5 0.12150457169669693 1104 6 0.19743148442696423 1104 7 0.15340170553344734 1104 8 0.26343236826306793 1104 9 0.10349766234934776 1104 10-11 0.4056296480773227 1104 12-13 0.3145467633373542 1104 14-15 0.5182077403926755 1104 16-17 0.49058317100298865 1104 18-19 0.21064617520350737 1104 20-21 0.19558392085965437 1104 22-23 0.023312694380173582 1104 24-25 0.34624449771563803 1104 26-27 0.2115021741202625 1104 28-29 0.11945457287482952 1104 30-31 0.43423731097119145 1104 32-33 0.48608258831198015 1104 34-35 0.5376767152499013 1104 36-37 0.5061973895810752 1104 38-39 0.7922491772670099 1104 40-41 0.9078538319555562 1104 42-43 0.8797058820279346 1104 44-45 1.3015365537171562 1104 46-47 1.2607651377189342 1104 48-49 1.2540458379353367 1104 50-51 1.016970239931993 1104 52-53 1.1751280007049019 1104 54-55 1.3174468539251194 1104 56-57 1.3184336188838373 1104 58-59 1.4868345188459173 1104 60-61 1.7631655641258135 1104 62-63 1.793935170114132 1104 64-65 1.8118980833371623 1104 66-67 1.6936931914095972 1104 68-69 1.765613225819454 1104 70-71 1.7841226796033531 1104 72-73 1.8263215914037225 1104 74-75 1.8442850184186206 1104 76-77 1.9784516020990566 1104 78-79 1.9468787662825378 1104 80-81 1.9224018398766525 1104 82-83 1.8242190768969522 1104 84-85 1.9281604472077554 1104 86-87 1.9780256908226157 1104 88-89 2.2752531840406185 1104 90-91 2.204330139570608 1104 92-93 2.0489288370398384 1104 94-95 2.1731161897507825 1104 96-97 2.245665032893534 1104 98-99 2.3086521705841996 1104 100-101 2.064256582402752 1105 1 0.08288096241224707 1105 2 0.3315531717594169 1105 3 0.26340409446918045 1105 4 0.26582421750336493 1105 5 0.2715973582875364 1105 6 0.2738936215116681 1105 7 0.17602966123533292 1105 8 0.07448893815232083 1105 9 -0.02069868604933589 1105 10-11 0.20371106082799173 1105 12-13 0.18585176184080154 1105 14-15 0.17043461857286246 1105 16-17 0.2968747004851693 1105 18-19 0.2420888570352986 1105 20-21 0.3396635377408259 1105 22-23 0.2613222723508386 1105 24-25 0.2997374638934289 1105 26-27 0.18744949528159083 1105 28-29 0.23945756599066215 1105 30-31 -0.19881267406322678 1105 32-33 0.153753944193447 1105 34-35 -0.22588209947623028 1105 36-37 -0.3265592701016544 1105 38-39 -0.458529032849718 1105 40-41 -0.25694712584150636 1105 42-43 -0.23490351635578577 1105 44-45 -0.06421621491498541 1105 46-47 0.04552718501016528 1105 48-49 0.013105999563592263 1105 50-51 0.22803279605291493 1105 52-53 0.30264072144719023 1105 54-55 0.28443577939653863 1105 56-57 0.5321540618649792 1105 58-59 0.7808063835570849 1105 60-61 0.6013427565831648 1105 62-63 0.505856750479289 1105 64-65 0.4893240037202773 1105 66-67 0.5519871153844527 1105 68-69 0.5916772784982918 1105 70-71 0.37377547816666024 1105 72-73 0.5519247042441897 1105 74-75 0.2521030369759423 1105 76-77 0.2488676451999261 1105 78-79 -0.15459983293925106 1105 80-81 -0.22636200328408052 1105 82-83 -0.03232986054693043 1105 84-85 -0.0027494600056563456 1105 86-87 0.39896552919436346 1105 88-89 0.552795835941243 1105 90-91 0.27439598811894683 1105 92-93 0.10736942369053892 1105 94-95 0.24807578268702812 1105 96-97 0.408861680603799 1105 98-99 0.39660787246986473 1105 100-101 0.4559057892270566 1106 1 0.34428402430893357 1106 2 0.3019135550674221 1106 3 0.2668998916075438 1106 4 0.12873714756106835 1106 5 0.057611534711107026 1106 6 -0.04973989949385782 1106 7 0.16979344020960951 1106 8 0.38051172231759267 1106 9 0.35682983807429736 1106 10-11 0.4474528812775276 1106 12-13 0.471209378374688 1106 14-15 0.5009148075918404 1106 16-17 0.7279820249706859 1106 18-19 0.7299984933142909 1106 20-21 0.48884316725659716 1106 22-23 0.3405222793775664 1106 24-25 0.3608963469950268 1106 26-27 0.3003821880115609 1106 28-29 0.40627867428132447 1106 30-31 0.6208617249319488 1106 32-33 0.4115140868360285 1106 34-35 0.3645650639215461 1106 36-37 0.5012902876536529 1106 38-39 0.593490712255722 1106 40-41 0.6349071398215074 1106 42-43 0.591402357110411 1106 44-45 0.6921824991937982 1106 46-47 0.31005494497713215 1106 48-49 0.17755339133001513 1106 50-51 0.24918763878596195 1106 52-53 0.6432613571903971 1106 54-55 0.7648839089693951 1106 56-57 0.8033198838603894 1106 58-59 0.9165254374081684 1106 60-61 0.8492742635528003 1106 62-63 0.6699066929479542 1106 64-65 0.7652701965512563 1106 66-67 0.8770479404979845 1106 68-69 1.0622133300042016 1106 70-71 0.9852808667886315 1106 72-73 1.115305790049323 1106 74-75 1.0424496303304132 1106 76-77 1.0244734808974592 1106 78-79 1.121438585695632 1106 80-81 0.8377986101492674 1106 82-83 1.0420919714967063 1106 84-85 1.063543326179797 1106 86-87 1.0471592209493785 1106 88-89 0.9685124304375599 1106 90-91 1.0437831696105455 1106 92-93 1.258470424120926 1106 94-95 1.3433384505599513 1106 96-97 1.432721813584628 1106 98-99 1.4752852666078162 1106 100-101 1.4053748320988788 1107 1 -0.00906127887270003 1107 2 -0.09040688277499953 1107 3 -0.011964858728745753 1107 4 -0.14456220064028003 1107 5 -0.24275275665270613 1107 6 -0.22614120886439792 1107 7 -0.038256763874379374 1107 8 -0.04453143264191084 1107 9 0.021416214385546084 1107 10-11 0.15357771024533662 1107 12-13 0.08498548063029432 1107 14-15 -0.06527248781900852 1107 16-17 -0.23543296120241308 1107 18-19 -0.1155096382924583 1107 20-21 0.014698616905299389 1107 22-23 -0.08194601110871957 1107 24-25 0.03512114536466271 1107 26-27 -0.27751612216146526 1107 28-29 -0.07593397719402617 1107 30-31 0.24782967768376807 1107 32-33 0.13131571835329225 1107 34-35 0.17939420492135838 1107 36-37 0.058898550312925124 1107 38-39 -0.4081344544465466 1107 40-41 0.05052548255559941 1107 42-43 0.2653875660727891 1107 44-45 0.3875113717380074 1107 46-47 -0.060832344083152634 1107 48-49 0.6445160327022066 1107 50-51 0.1636513337742045 1107 52-53 0.35784686751583195 1107 54-55 0.5913461260085313 1107 56-57 0.4883175456987132 1107 58-59 0.35341527823997865 1107 60-61 0.24768258163516066 1107 62-63 0.16834791849107233 1107 64-65 0.4031611169787084 1107 66-67 0.48117696083710015 1107 68-69 0.3705591239111321 1107 70-71 0.5077878379743943 1107 72-73 0.7080095709433643 1107 74-75 0.7901850774388883 1107 76-77 0.5763229379243597 1107 78-79 0.7261882291981401 1107 80-81 0.6320201755052146 1107 82-83 0.020069952364195842 1107 84-85 0.13970677811469656 1107 86-87 -0.11991253463478913 1107 88-89 0.014828238142523986 1107 90-91 0.23987410966701006 1107 92-93 0.25436853800383474 1107 94-95 0.08546519624301041 1107 96-97 0.08220251076093632 1107 98-99 0.12808557605341164 1107 100-101 -0.03263501704639182 1108 1 0.32602765721757976 1108 2 0.08878019367680423 1108 3 0.1576422619382427 1108 4 0.10648899360307951 1108 5 -0.07316731009382949 1108 6 0.022316317108405315 1108 7 0.0962960992752997 1108 8 0.22981220311748274 1108 9 0.21417128251449213 1108 10-11 0.028745684583185493 1108 12-13 0.26145245651424176 1108 14-15 0.45116732318276576 1108 16-17 0.49906643914726345 1108 18-19 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-0.6849865655798766 2316 54-55 -0.8485760593318403 2316 56-57 -0.7984403582029032 2316 58-59 -0.5822162176516272 2316 60-61 -0.8970635684437411 2316 62-63 -1.063871703862958 2316 64-65 -1.0531919330294528 2316 66-67 -1.34483381350039 2316 68-69 -1.0526682799088611 2316 70-71 -0.8128003973197231 2316 72-73 -1.3283592596601075 2316 74-75 -1.6385791387008517 2316 76-77 -1.9666220476554415 2316 78-79 -1.9346105954195885 2316 80-81 -1.6827863761626318 2316 82-83 -1.6661737217937187 2316 84-85 -1.5253092745598416 2316 86-87 -2.223267271534173 2316 88-89 -1.9785602365813126 2316 90-91 -1.2820201059285736 2316 92-93 -1.3294672349732544 2316 94-95 -1.9228903568940652 2316 96-97 -2.3181811209526195 2316 98-99 -2.749269269677665 2316 100-101 -2.769294972425403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1095.0 3 362.0 4 285.0 5 304.0 6 328.0 7 443.0 8 472.0 9 633.0 10 875.0 11 1176.0 12 1932.0 13 2793.0 14 3320.0 15 4693.0 16 7065.0 17 10876.0 18 9460.0 19 4635.0 20 3734.0 21 3532.0 22 3486.0 23 3714.0 24 3912.0 25 4197.0 26 4596.0 27 5253.0 28 6040.0 29 7059.0 30 8559.0 31 10878.0 32 14272.0 33 19021.0 34 27488.0 35 42579.0 36 62575.0 37 96009.0 38 100561.0 39 22303.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.53679746256098 19.478681794834387 15.9980973204596 18.986423422145034 2 14.92907808505833 21.45945049256604 42.4734030327438 21.13806838963183 3 17.970223820943247 29.06554756195044 32.14488409272582 20.819344524380494 4 11.253185684160028 18.175040227070568 39.72135882546948 30.85041526329992 5 11.272229275168186 38.86022171796436 34.098783450123456 15.768765556744006 6 26.516222283974432 39.141595379512886 18.610495254964444 15.731687081548241 7 24.288019128769324 37.35448000655752 21.633222641841385 16.72427822283177 8 25.909627991690943 33.27422092579227 20.398378973843258 20.417772108673528 9 27.910705574703908 16.080353786976097 21.424144521659855 34.58479611666014 10-11 23.28634151608092 28.535082913799236 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36-37 19.05055893547517 25.922318458357058 35.98165852488986 19.04546408127791 38-39 18.788861449442155 26.334076596016754 35.7411447843739 19.135917170167193 40-41 18.820779220779222 26.516283716283716 35.575224775224775 19.08771228771229 42-43 18.75877239864976 26.64911104695362 35.10867534657531 19.483441207821308 44-45 18.94085832991188 26.839567368638722 34.73158334806805 19.487990953381342 46-47 19.263723518711096 27.16552934606813 34.01392755712326 19.55681957809751 48-49 19.31429382013324 27.61565210615749 33.151689091655584 19.91836498205368 50-51 19.36475778318568 28.118868655869505 32.47369640880556 20.04267715213925 52-53 19.4341160698409 28.422465929683582 32.02999346665095 20.113424533824567 54-55 19.198686121761508 28.249880620096185 32.12428297133084 20.427150286811468 56-57 19.378021743061794 28.42384283289255 31.80989358268948 20.388241841356177 58-59 19.496481504421595 27.940271489596363 32.093534839300055 20.469712166681987 60-61 19.42208416965812 27.902092801828744 32.48083987823044 20.194983150282695 62-63 19.470281114066093 27.69380166362621 32.71255392398791 20.12336329831979 64-65 19.341200353256635 27.84763210159725 32.537373672790046 20.27379387235607 66-67 19.26746565214959 27.991400049553615 32.232330538615855 20.508803759680937 68-69 19.331113904270136 27.847806581952682 32.32846217945003 20.49261733432715 70-71 19.445637774318786 27.955089945990448 32.013299578193966 20.5859727014968 72-73 19.4790014027039 27.901019458020787 31.76894549436321 20.8510336449121 74-75 19.266071544536747 28.323983555047132 31.590095063017337 20.81984983739878 76-77 19.148366443423757 28.153984651092617 31.525009167180396 21.17263973830323 78-79 19.162802781996962 28.037912702853944 31.538392357502598 21.260892157646495 80-81 19.10587059705221 28.222233325006247 31.41603797152136 21.255858106420185 82-83 19.077662820930453 28.19421600740236 31.26007981917779 21.468041352489404 84-85 19.123870993525696 27.977379905682998 31.020202222044603 21.8785468787467 86-87 19.089077967700295 28.01146195932363 31.036012453091093 21.863447619884983 88-89 19.16610383537884 27.791709794862985 31.006142520871382 22.036043848886795 90-91 19.12331252547697 27.881195399358976 31.1086777553092 21.886814319854853 92-93 19.262436774442385 28.11651288692243 30.68290596753339 21.9381443711018 94-95 19.092008112779627 28.268540998511327 30.475576736704333 22.163874152004716 96-97 19.276786972332395 28.279576017211934 30.190336574061583 22.25330043639409 98-99 19.521804835924005 28.69640743939103 29.65891463570652 22.12287308897844 100-101 19.81369433595995 28.605786291269787 29.21794204112559 22.362577331644673 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 602.5 2 502.5 3 618.5 4 508.0 5 437.5 6 809.0 7 1992.0 8 3650.5 9 4176.0 10 3200.5 11 2276.5 12 2074.0 13 2152.0 14 2239.5 15 2276.5 16 2311.0 17 2345.5 18 2382.5 19 2503.5 20 2688.5 21 2886.0 22 3229.5 23 3637.0 24 4072.0 25 4734.0 26 5642.0 27 6487.0 28 7033.5 29 7802.5 30 8795.0 31 9725.0 32 10674.5 33 11578.5 34 12470.0 35 13089.0 36 13961.0 37 14888.0 38 15001.0 39 14708.5 40 14906.0 41 15206.5 42 15048.0 43 15358.5 44 16026.0 45 16268.5 46 16177.0 47 16255.5 48 16971.0 49 16699.0 50 14991.5 51 13747.5 52 12847.5 53 12099.5 54 11398.5 55 10365.5 56 9306.0 57 8236.0 58 7418.5 59 6642.5 60 5599.0 61 4695.5 62 3900.0 63 2879.0 64 1968.5 65 1406.5 66 1009.0 67 773.5 68 542.5 69 341.0 70 221.5 71 155.5 72 116.0 73 98.0 74 80.5 75 67.5 76 51.0 77 23.5 78 13.5 79 11.0 80 8.0 81 6.5 82 5.5 83 2.5 84 2.0 85 2.5 86 3.5 87 3.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035562659207831776 2 0.03716098096998152 3 0.02497377753358973 4 0.04794965286449228 5 0.06792867489136407 6 0.06353329004545227 7 0.0671295140102892 8 0.06952699665351382 9 0.06732930423055791 10-11 0.07332301083861945 12-13 0.0756205983717097 14-15 0.06643024823934869 16-17 0.05793916387792817 18-19 0.042455421807102545 20-21 0.05064682083811997 22-23 0.02697167973627691 24-25 0.014584686079616401 26-27 0.011487937665451276 28-29 0.013286049647869736 30-31 0.008191399031017432 32-33 0.00649318215873333 34-35 0.006393287048598971 36-37 0.003995804405374357 38-39 0.0057939163877928175 40-41 0.004994755506717946 42-43 0.004095699515508716 44-45 0.001298636431746666 46-47 0.001298636431746666 48-49 8.990559912092302E-4 50-51 0.004595175066180511 52-53 0.0031966435242994857 54-55 0.0043953848459117924 56-57 0.017881224714050247 58-59 0.005394335947255382 60-61 0.014285000749213328 62-63 0.010389091453973327 64-65 0.0077918185904799955 66-67 0.011388042555316917 68-69 0.019179861145796912 70-71 0.011587832775585635 72-73 0.012586783876929224 74-75 0.013485839868138455 76-77 0.019679336696468708 78-79 0.032965386344338446 80-81 0.029968533040307676 82-83 0.028769791718695367 84-85 0.01698216872284102 86-87 0.017381749163378455 88-89 0.01578342740122871 90-91 0.014584686079616401 92-93 0.02677188951600819 94-95 0.015983217621497427 96-97 0.034863393436891266 98-99 0.0537435692522851 100-101 0.06503171669746766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 500525.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.3210279767023 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13954178576049 53.4762079317211 2 10.336432875356225 13.296999763101864 3 2.8971697223843167 5.590468043002094 4 1.2250207662195949 3.151783799042074 5 0.6349738280502545 2.0421084679247086 6 0.39828748058844815 1.5370956109019906 7 0.2522623747494073 1.1358042684608192 8 0.1910543490894781 0.9831049706283974 9 0.13048610152627682 0.755370016815822 >10 0.732788223232262 8.509436176078525 >50 0.03966879601523794 1.7368396998586715 >100 0.019214572440827186 2.3111349663551475 >500 0.0012396498348920726 0.5457898514645836 >1k 0.0012396498348920726 1.689397001885444 >5k 6.198249174460363E-4 3.238459432758811 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9567 1.9113930373108237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6679 1.3343988811747665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 0.9438090005494232 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.28550022476399783 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1171 0.2339543479346686 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1151 0.22995854352929426 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 0.15503721092852504 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 685 0.13685630088407172 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.13525797912192197 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.1198741321612307 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9979022026871785E-4 0.0 10-11 0.0 0.0 0.0 1.9979022026871785E-4 0.0 12-13 0.0 0.0 0.0 1.9979022026871785E-4 0.0 14-15 0.0 0.0 0.0 2.996853304030768E-4 0.0 16-17 0.0 0.0 0.0 3.995804405374357E-4 0.0 18-19 0.0 0.0 0.0 3.995804405374357E-4 0.0 20-21 0.0 0.0 0.0 3.995804405374357E-4 0.0 22-23 0.0 0.0 0.0 5.993706608061536E-4 0.0 24-25 0.0 0.0 0.0 0.001198741321612307 0.0 26-27 0.0 0.0 0.0 0.002097797312821537 0.0 28-29 0.0 0.0 0.0 0.006692972379002048 0.0 30-31 0.0 0.0 0.0 0.01927975625593127 0.0 32-33 0.0 0.0 0.0 0.03506318365715998 0.0 34-35 0.0 0.0 0.0 0.05823884920833125 0.0 36-37 0.0 0.0 0.0 0.09280255731481944 0.0 38-39 0.0 0.0 0.0 0.14524749013535787 0.0 40-41 0.0 0.0 0.0 0.20498476599570453 0.0 42-43 0.0 0.0 0.0 0.28560011987413214 0.0 44-45 0.0 0.0 0.0 0.3817991109335198 0.0 46-47 0.0 0.0 0.0 0.5013735577643474 0.0 48-49 0.0 0.0 0.0 0.6517156985165575 0.0 50-51 0.0 0.0 0.0 0.8252335048199391 0.0 52-53 0.0 0.0 0.0 1.020028969581939 0.0 54-55 0.0 0.0 0.0 1.197043104740023 0.0 56-57 0.0 0.0 0.0 1.4508765795914291 0.0 58-59 0.0 0.0 0.0 1.7403726087608011 0.0 60-61 0.0 0.0 0.0 2.0528445132610758 0.0 62-63 0.0 0.0 0.0 2.4236551620798163 0.0 64-65 0.0 0.0 0.0 2.768792767594026 0.0 66-67 0.0 0.0 0.0 3.1769641876030166 0.0 68-69 0.0 0.0 0.0 3.6487687927675942 0.0 70-71 0.0 0.0 0.0 4.181309624893862 0.0 72-73 0.0 0.0 0.0 4.776384795964238 0.0 74-75 0.0 0.0 0.0 5.349882623245592 0.0 76-77 0.0 0.0 0.0 5.940162829029519 0.0 78-79 0.0 0.0 0.0 6.622246641026922 0.0 80-81 0.0 0.0 0.0 7.460366615054193 0.0 82-83 0.0 0.0 0.0 8.22436441736177 0.0 84-85 0.0 0.0 0.0 9.088457120023975 0.0 86-87 0.0 0.0 0.0 10.026671994405874 0.0 88-89 0.0 0.0 0.0 11.036411767643974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3100 0.0 52.88616 1 GTATCAA 5880 0.0 44.611454 1 TATCAAC 7450 0.0 35.082542 2 ATCAACG 7555 0.0 34.657864 3 TCAACGC 7645 0.0 34.249855 4 CAACGCA 7765 0.0 33.66946 5 AACGCAG 7910 0.0 33.05226 6 TAGGACA 140 1.6945705E-8 30.549149 4 CGCAGAG 8630 0.0 29.909143 8 ACGCAGA 8700 0.0 29.887049 7 GCAGAGT 8780 0.0 28.965042 9 GAGTACT 5430 0.0 26.34479 12-13 GTACATG 4355 0.0 26.188326 1 TACATGG 4370 0.0 25.772203 2 AGTACTT 5465 0.0 25.045538 12-13 ACATGGG 4405 0.0 24.488636 3 GTACTTT 5910 0.0 24.084488 14-15 GTATAGA 160 1.84897E-6 23.760448 1 CAGAGTA 8570 0.0 23.350409 10-11 CATGGGA 3430 0.0 23.275541 4 >>END_MODULE