FastQCFastQC Report
Thu 26 May 2016
SRR936782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500525
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78111.5605614105189551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59841.1955446780880075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43490.868887667948654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.2876979171869537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13630.2723140702262624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.22796064132660707No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.22776085110633834No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7280.1454472803556266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.12946406273412916No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.10808650916537636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.045.750751
GTATCAA59650.038.9481661
GTTCCGA600.003952337431.6609386
TATCAAC77250.029.9425582
ATCAACG78100.029.6774923
TCAACGC78900.029.3134564
GGATACG650.005834718729.2371651
CAACGCA79950.028.9284765
AACGCAG82100.028.2287526
AGTCCCG700.00839452627.1379495
ATAGTAC1109.471364E-525.9069923
ACGCAGA91550.025.3149197
CGCAGAG92150.025.2016288
GCAGAGT93900.024.4284889
GAGTACT55450.023.76830312-13
GTATAGG1801.9423351E-723.75521
AGTACTT55350.023.51092312-13
GTACATG44050.022.8653891
TACATGG43550.022.793792
GTACTTT58550.022.14594314-15
Produced by FastQC (version 0.11.5)