##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 500525 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84499475550672 31.0 31.0 34.0 30.0 34.0 2 31.90249837670446 31.0 31.0 34.0 30.0 34.0 3 31.988865691024426 31.0 31.0 34.0 30.0 34.0 4 35.80878677388742 37.0 35.0 37.0 35.0 37.0 5 35.71669546975676 37.0 35.0 37.0 33.0 37.0 6 35.775313920383596 37.0 35.0 37.0 33.0 37.0 7 35.71764846910744 37.0 35.0 37.0 33.0 37.0 8 35.7015653563758 37.0 35.0 37.0 33.0 37.0 9 37.287869736776386 39.0 37.0 39.0 34.0 39.0 10-11 37.19014634633635 39.0 37.0 39.0 33.0 39.0 12-13 37.08529444083712 39.0 37.0 39.0 33.0 39.0 14-15 38.07252085310424 40.0 37.0 41.0 33.0 41.0 16-17 38.035647570051445 40.0 37.0 41.0 33.0 41.0 18-19 38.18887667948654 40.0 37.5 41.0 33.5 41.0 20-21 38.30904050746716 40.0 38.0 41.0 34.0 41.0 22-23 38.23101043903901 40.0 37.5 41.0 33.5 41.0 24-25 38.120978972079314 40.0 37.0 41.0 33.0 41.0 26-27 37.87114529743769 40.0 37.0 41.0 33.0 41.0 28-29 37.657194945307424 39.5 36.5 41.0 33.0 41.0 30-31 37.41670745716998 39.0 36.0 41.0 32.5 41.0 32-33 37.13733579741272 39.0 35.0 41.0 32.0 41.0 34-35 36.92187303331502 39.0 35.0 41.0 31.5 41.0 36-37 36.645855851356075 39.0 35.0 41.0 31.0 41.0 38-39 36.40755506717946 39.0 35.0 40.0 30.5 41.0 40-41 36.08033564757005 38.5 35.0 40.0 30.0 41.0 42-43 35.685255481744164 38.0 35.0 40.0 29.5 41.0 44-45 35.16132361020928 38.0 35.0 40.0 26.5 41.0 46-47 34.46070026472204 38.0 34.0 40.0 23.5 41.0 48-49 33.77823185655062 38.0 33.0 40.0 18.5 41.0 50-51 33.399381649268264 38.0 33.0 40.0 12.5 41.0 52-53 33.09911492932421 38.0 33.0 40.0 8.5 41.0 54-55 32.79275660556416 37.5 33.0 40.0 7.0 41.0 56-57 32.49274461815094 37.0 32.0 40.0 2.0 41.0 58-59 32.11477848259328 37.0 31.5 40.0 2.0 41.0 60-61 31.728821737175963 36.0 31.0 39.0 2.0 41.0 62-63 31.364197592527844 36.0 31.0 39.0 2.0 40.0 64-65 30.914559712302083 35.0 30.0 39.0 2.0 40.0 66-67 30.448102492383 35.0 29.0 38.0 2.0 40.0 68-69 29.96499775236002 35.0 29.0 38.0 2.0 40.0 70-71 29.550092402976873 34.0 28.5 37.0 2.0 39.5 72-73 29.15981319614405 34.0 28.0 37.0 2.0 39.0 74-75 28.656835322910943 34.0 27.5 36.0 2.0 39.0 76-77 27.307862744118676 32.5 26.0 35.0 2.0 37.0 78-79 27.950971479946055 34.0 26.0 35.0 2.0 37.5 80-81 27.555000249737773 34.0 26.0 35.0 2.0 37.0 82-83 27.385817891214224 33.5 26.0 35.0 2.0 37.0 84-85 27.033726587083564 33.0 25.5 35.0 2.0 36.0 86-87 26.62093801508416 33.0 25.0 35.0 2.0 36.0 88-89 26.17885520203786 33.0 24.0 35.0 2.0 35.0 90-91 25.665175565656064 32.0 21.5 35.0 2.0 35.0 92-93 25.159922081814095 32.0 19.5 34.0 2.0 35.0 94-95 24.56533939363668 32.0 14.5 34.0 2.0 35.0 96-97 23.818670396084112 31.0 2.0 34.0 2.0 35.0 98-99 22.936851306128567 31.0 2.0 34.0 2.0 35.0 100-101 20.025930772688675 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34066290352048156 1101 2 0.29354498978215915 1101 3 0.3609593534584903 1101 4 0.2988872836294405 1101 5 0.35453809499466615 1101 6 0.41887185343345834 1101 7 0.4857678296866297 1101 8 0.3983581927549693 1101 9 0.5464766038194071 1101 10-11 0.4703872366152595 1101 12-13 0.3822499319661432 1101 14-15 0.6280269697444751 1101 16-17 0.6408092038572875 1101 18-19 0.6896778025420716 1101 20-21 0.6350535514311844 1101 22-23 0.729618617885464 1101 24-25 0.7508367934231117 1101 26-27 0.5775063788249 1101 28-29 0.6788187744459222 1101 30-31 0.6617376848152787 1101 32-33 0.7606920608579344 1101 34-35 0.8706310610770558 1101 36-37 0.7997628486668305 1101 38-39 0.7843376458629123 1101 40-41 0.7948181197042814 1101 42-43 0.8457069545503657 1101 44-45 0.8190849821176158 1101 46-47 1.1881775889618424 1101 48-49 1.1324961755962377 1101 50-51 1.1032940894230663 1101 52-53 1.1495548287480233 1101 54-55 1.1522716326793017 1101 56-57 1.2587318233007494 1101 58-59 1.2829508811408203 1101 60-61 1.1839499113322383 1101 62-63 1.2207345364682922 1101 64-65 1.3956167144437366 1101 66-67 1.4557094981992371 1101 68-69 1.2838171960308493 1101 70-71 1.4176906101220155 1101 72-73 1.3948779936675315 1101 74-75 1.114008313167595 1101 76-77 0.9096138199361157 1101 78-79 1.3732693206665516 1101 80-81 1.6563939043721838 1101 82-83 1.4081710937267786 1101 84-85 1.1149779080696298 1101 86-87 1.101295508952962 1101 88-89 1.1353223341079364 1101 90-91 1.007569834756655 1101 92-93 1.3165332476010434 1101 94-95 1.3880821125129543 1101 96-97 1.2957458026028412 1101 98-99 1.4263793999794565 1101 100-101 1.4032731312420061 1102 1 0.3576128320077885 1102 2 0.31507930798047923 1102 3 0.3330504603107656 1102 4 0.2755643588393255 1102 5 0.2987345165185715 1102 6 0.35033333287790214 1102 7 0.3518954411627604 1102 8 0.27563104918969117 1102 9 0.3761501081306875 1102 10-11 0.43576593814685793 1102 12-13 0.40791004303971334 1102 14-15 0.4037707490875704 1102 16-17 0.615930522848096 1102 18-19 0.49979187554500015 1102 20-21 0.22784087300465927 1102 22-23 0.34974494118596766 1102 24-25 0.3371461386952248 1102 26-27 0.2710184570499905 1102 28-29 0.48517330058357544 1102 30-31 0.2388608855528176 1102 32-33 0.398736023008297 1102 34-35 0.3340356650419807 1102 36-37 0.4547448994550507 1102 38-39 0.6040617300110895 1102 40-41 0.543125021231468 1102 42-43 0.7302036978024375 1102 44-45 0.8724748099504183 1102 46-47 1.0161635200633938 1102 48-49 1.2957455986009094 1102 50-51 1.2192835693221582 1102 52-53 1.3519602125643715 1102 54-55 1.0186297265582525 1102 56-57 1.067349339331905 1102 58-59 1.3131559157605395 1102 60-61 1.1452912241978943 1102 62-63 0.9271591545147473 1102 64-65 0.8550896990645604 1102 66-67 0.7911750568664822 1102 68-69 0.9752415141344528 1102 70-71 1.0712779266438126 1102 72-73 1.205039426068307 1102 74-75 1.390070979122818 1102 76-77 1.274229087974522 1102 78-79 1.3701286032663944 1102 80-81 1.3060346044072304 1102 82-83 1.166035121738279 1102 84-85 1.193363236759641 1102 86-87 1.2305140473156548 1102 88-89 1.0308530277336772 1102 90-91 1.3189641901478915 1102 92-93 1.445218992738024 1102 94-95 1.4382964927205144 1102 96-97 1.6493310668840664 1102 98-99 1.7021429124049838 1102 100-101 1.7615114563611165 1103 1 0.20402129675332148 1103 2 0.05512397954145243 1103 3 0.22560348258605956 1103 4 0.16074679758394694 1103 5 0.0119695305988472 1103 6 0.18015126378259794 1103 7 0.25523214241217573 1103 8 0.3118779659782689 1103 9 0.36488924548117296 1103 10-11 0.06474050851353041 1103 12-13 0.21263494553070217 1103 14-15 0.32201888011817914 1103 16-17 0.31609115228248896 1103 18-19 0.3717543102122818 1103 20-21 0.2845775639486945 1103 22-23 0.44473529669901524 1103 24-25 0.34171690861876414 1103 26-27 0.39301113575303503 1103 28-29 0.15262276504011396 1103 30-31 0.2724877727246451 1103 32-33 0.2301742501253301 1103 34-35 0.49815268481710007 1103 36-37 0.43589222375400993 1103 38-39 0.4785338595720603 1103 40-41 0.5827659909224607 1103 42-43 0.6696495374552782 1103 44-45 0.8136753637523242 1103 46-47 0.45536460705644544 1103 48-49 0.2952409712634321 1103 50-51 0.2909977788662772 1103 52-53 0.6581026642131178 1103 54-55 0.46116157800851454 1103 56-57 0.5468186797206869 1103 58-59 0.5839122854905128 1103 60-61 0.5380341118088161 1103 62-63 0.5964596659749972 1103 64-65 0.5055436956458621 1103 66-67 0.685232999958405 1103 68-69 0.7914653110379106 1103 70-71 0.44050775667233566 1103 72-73 0.5355820936818709 1103 74-75 0.39438075421920615 1103 76-77 0.5380567731408306 1103 78-79 0.7422052931799001 1103 80-81 1.1763016928475132 1103 82-83 1.066647619469979 1103 84-85 0.8567129541459266 1103 86-87 0.7708741529681511 1103 88-89 0.5096659605314287 1103 90-91 0.5443724286232303 1103 92-93 0.43900720383691194 1103 94-95 0.9048919552190284 1103 96-97 1.2505544941381999 1103 98-99 1.6824814887846173 1103 100-101 1.2839452499265285 1104 1 0.2330704294388184 1104 2 0.06374081724919733 1104 3 0.08360366422260412 1104 4 0.20074316485304422 1104 5 0.15417459825845725 1104 6 0.1862288225874309 1104 7 0.04048688766691555 1104 8 0.20597114552599294 1104 9 0.15506997384372312 1104 10-11 0.02975367716306465 1104 12-13 0.3436975193205498 1104 14-15 0.3310616555245858 1104 16-17 0.28772588118994236 1104 18-19 0.2124309063434211 1104 20-21 0.31981868646254696 1104 22-23 0.20024705766531525 1104 24-25 0.17598718379812794 1104 26-27 0.330155809261349 1104 28-29 0.30051850566312766 1104 30-31 0.371809360228049 1104 32-33 0.4323457150240628 1104 34-35 0.47789203637464084 1104 36-37 0.5393033580742355 1104 38-39 1.077394090250472 1104 40-41 1.1791314436515492 1104 42-43 1.011583058479708 1104 44-45 1.0486549296409748 1104 46-47 1.562988801044277 1104 48-49 1.8110805861939596 1104 50-51 1.764246348478487 1104 52-53 1.8848050527336966 1104 54-55 1.6201725670195017 1104 56-57 1.6345336919811544 1104 58-59 1.840451201120345 1104 60-61 1.7902291894584401 1104 62-63 1.6157812534784028 1104 64-65 2.1105341597272336 1104 66-67 2.0916611172956117 1104 68-69 2.029578107332526 1104 70-71 2.0413114983851663 1104 72-73 2.2149445494079636 1104 74-75 2.249158703542612 1104 76-77 2.1216104921490953 1104 78-79 2.1001373611119654 1104 80-81 2.5187655925841383 1104 82-83 2.435877480518023 1104 84-85 1.8344915391972414 1104 86-87 1.8277027788876872 1104 88-89 1.97144418230965 1104 90-91 2.053824794439233 1104 92-93 2.1456641832211645 1104 94-95 1.93320818444435 1104 96-97 2.001054902820428 1104 98-99 2.5695126394521317 1104 100-101 2.0859750383199547 1105 1 0.11150203674202075 1105 2 0.16392639043088053 1105 3 0.08161623530910589 1105 4 -0.02760164209098548 1105 5 0.08266307476250034 1105 6 0.03611807730158745 1105 7 -0.02345803900174559 1105 8 0.22852128021620643 1105 9 0.015866142655454496 1105 10-11 -0.10098861556366501 1105 12-13 0.2345146399431215 1105 14-15 0.010298410987019224 1105 16-17 0.07678005658652864 1105 18-19 0.09902474052480414 1105 20-21 0.1210787882284805 1105 22-23 0.0019112300687922357 1105 24-25 0.07507428346888645 1105 26-27 0.2168963061185778 1105 28-29 0.3460497837235934 1105 30-31 0.06193806420889558 1105 32-33 -0.03522686803489705 1105 34-35 0.11109766343240324 1105 36-37 0.16278135867285215 1105 38-39 0.08800468588052723 1105 40-41 0.022447815995157328 1105 42-43 0.31450733264912145 1105 44-45 0.09687103260416308 1105 46-47 0.05661346431874392 1105 48-49 0.2531009393816319 1105 50-51 0.2327767286042004 1105 52-53 0.40221900065347427 1105 54-55 0.18086696989288953 1105 56-57 0.4258446767162596 1105 58-59 0.6432048676872313 1105 60-61 0.3937850110687364 1105 62-63 0.3499177395604143 1105 64-65 0.14853176523456924 1105 66-67 0.4279137362719716 1105 68-69 0.6798594602208823 1105 70-71 0.4545261047904354 1105 72-73 0.4230379346219699 1105 74-75 5.026125518909907E-4 1105 76-77 0.20480115362769524 1105 78-79 0.35024511328197505 1105 80-81 0.32100444322765753 1105 82-83 0.3021480581893776 1105 84-85 -0.12672366583119654 1105 86-87 0.08295869029145919 1105 88-89 -0.06322807150657184 1105 90-91 0.14160390249071853 1105 92-93 0.5690404178814426 1105 94-95 0.486713123085476 1105 96-97 0.7680228764810089 1105 98-99 0.9351666352617691 1105 100-101 0.6476631556500934 1106 1 0.04148508476092516 1106 2 0.09938589451955693 1106 3 0.01668926936238435 1106 4 0.15844415460821182 1106 5 0.01463995682765784 1106 6 0.17919982449748773 1106 7 0.09164854767733743 1106 8 0.14791592760621342 1106 9 0.20630803600729308 1106 10-11 0.2762478601809448 1106 12-13 -0.05627990731601784 1106 14-15 0.23333114675570243 1106 16-17 0.17056491574454924 1106 18-19 0.35254030033544126 1106 20-21 0.067212331192529 1106 22-23 0.11562125457450634 1106 24-25 -0.005702345634063022 1106 26-27 0.03798702995938896 1106 28-29 0.07163849177182868 1106 30-31 -0.07852403098569738 1106 32-33 -0.1913893084175129 1106 34-35 0.30697868336196166 1106 36-37 0.3350942940006121 1106 38-39 0.48407059658837426 1106 40-41 0.5498520548687722 1106 42-43 0.40651794300827504 1106 44-45 0.5772466990453893 1106 46-47 0.6966144304938311 1106 48-49 0.45159282790258715 1106 50-51 0.3546509326076759 1106 52-53 0.3534315503895371 1106 54-55 0.6288494206399236 1106 56-57 0.5431515075741373 1106 58-59 0.8562091452078562 1106 60-61 1.0129275745947517 1106 62-63 0.9677954920614908 1106 64-65 1.0141719440647954 1106 66-67 1.1525362692317138 1106 68-69 0.9947908178725484 1106 70-71 0.877732752075044 1106 72-73 1.1529129466991606 1106 74-75 1.2096136436363771 1106 76-77 0.8583373544515815 1106 78-79 0.8208162984274736 1106 80-81 1.3452927674408848 1106 82-83 1.2376295208027948 1106 84-85 0.970091469740737 1106 86-87 0.9277097245379728 1106 88-89 0.9957261757112832 1106 90-91 1.2672264266670545 1106 92-93 0.9689842040581134 1106 94-95 0.8376169359637089 1106 96-97 1.190768394746108 1106 98-99 1.4868767651684003 1106 100-101 0.9181768094607783 1107 1 -0.1591349610785926 1107 2 -0.10262550185369435 1107 3 -0.1633607268850099 1107 4 0.05767804582884395 1107 5 -0.1695436783498394 1107 6 -0.1556480217958054 1107 7 0.07819690143831082 1107 8 -0.12957479191252474 1107 9 -0.08416669741340854 1107 10-11 0.04258468247488878 1107 12-13 0.13645966765815132 1107 14-15 -0.02329797461509031 1107 16-17 0.02439517097943167 1107 18-19 0.28825817370522344 1107 20-21 0.04840023279344763 1107 22-23 -0.0028456041485682704 1107 24-25 -0.03421348509819211 1107 26-27 -0.05508912483269768 1107 28-29 0.043398694527972737 1107 30-31 0.12670017274034961 1107 32-33 0.28453339946239 1107 34-35 0.11192115303802552 1107 36-37 -0.15339386797337795 1107 38-39 -0.07539953556105417 1107 40-41 0.15045939958309162 1107 42-43 -0.38377204677309606 1107 44-45 -0.5007410963279426 1107 46-47 -0.6613177108581496 1107 48-49 -0.36573035222823336 1107 50-51 -0.2789013989147904 1107 52-53 -0.7337587875181271 1107 54-55 -1.0379250131496924 1107 56-57 -1.1683366601730896 1107 58-59 -0.9319764990567201 1107 60-61 -0.5535166299395549 1107 62-63 -0.5275888035745169 1107 64-65 -0.6441677879414662 1107 66-67 -1.091899771943023 1107 68-69 -1.0391962385262197 1107 70-71 -1.054763654083846 1107 72-73 -1.0849531488017554 1107 74-75 -0.8743667735378509 1107 76-77 -0.874067257211518 1107 78-79 -0.7570473723131776 1107 80-81 -1.0528673013761214 1107 82-83 -1.0345671388012754 1107 84-85 -1.0253274653276456 1107 86-87 -0.7081461292374378 1107 88-89 -0.6149063980562737 1107 90-91 -0.7745718217323159 1107 92-93 -0.977786534858712 1107 94-95 -0.8700320280286 1107 96-97 -0.6271725219287347 1107 98-99 -0.30748913115577636 1107 100-101 -0.6864920086995205 1108 1 0.11127121170739684 1108 2 0.042193663837636564 1108 3 0.027306402162629695 1108 4 0.09843981848189998 1108 5 0.06676912237841037 1108 6 0.018597358006815057 1108 7 -0.0019033774352124055 1108 8 -0.09814011044966975 1108 9 -0.01980182102807504 1108 10-11 -0.03919895212854385 1108 12-13 0.08431898824710515 1108 14-15 0.45638977373405964 1108 16-17 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-0.6788150397657979 2316 78-79 -0.26654021662944416 2316 80-81 -0.4503342437497402 2316 82-83 -0.5547771839664293 2316 84-85 -0.8845264640760853 2316 86-87 -0.9743266810141122 2316 88-89 -0.6480157194906759 2316 90-91 -0.7449968094887502 2316 92-93 -0.7533538200521583 2316 94-95 -0.7294595733297911 2316 96-97 -0.4582085996345633 2316 98-99 -0.3498817958997513 2316 100-101 -0.8560871548060689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 25.0 6 52.0 7 141.0 8 288.0 9 588.0 10 1008.0 11 1563.0 12 2424.0 13 3275.0 14 4314.0 15 5983.0 16 8168.0 17 11462.0 18 9980.0 19 5519.0 20 4496.0 21 4382.0 22 4463.0 23 4828.0 24 5364.0 25 6136.0 26 6992.0 27 8455.0 28 10316.0 29 12127.0 30 14643.0 31 17879.0 32 22294.0 33 28015.0 34 36733.0 35 49949.0 36 67772.0 37 87019.0 38 51198.0 39 2666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87319946923311 20.902143850617897 16.522117951751373 18.702538728397627 2 15.568729469873164 23.0263105314056 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25.66700364819129 35.570764122256364 17.87453898130352 22-23 20.05803905898806 26.426751910493984 35.35068178412667 18.16452724639129 24-25 19.3620218060631 26.4394019616266 35.78390139519643 18.414674837113864 26-27 19.35034965034965 26.661438561438562 35.663336663336665 18.324875124875124 28-29 18.843908345454835 26.959287735170474 35.69136522753792 18.50543869183677 30-31 19.114824240861815 26.676379158910162 35.64724467986247 18.561551920365556 32-33 18.927563913019156 27.03027227465616 35.34715618032302 18.695007632001662 34-35 19.193803127007325 26.99883419029171 35.30934915451324 18.49801352818772 36-37 19.565736482591394 26.756071706531937 35.105248907747225 18.572942903129444 38-39 19.307227922672787 27.144745267948927 35.00374128490409 18.544285524474198 40-41 19.21355150597264 27.291127404016656 34.80016104201778 18.69516004799292 42-43 19.24788729298768 27.529079380239306 34.22570374447909 18.997329582293922 44-45 19.409186174984466 27.685494682455804 33.81270816142637 19.092610981133358 46-47 19.8691347911651 28.107848915616913 32.894818337112774 19.12819795610521 48-49 19.899903599774234 28.575524332316053 31.998881158001467 19.525690909908246 50-51 19.96429666732266 28.989228062779524 31.450400438749405 19.59607483114841 52-53 20.02565367932501 29.31102688641454 31.06862359219441 19.59469584206604 54-55 19.739289574579885 29.25065482291311 31.029015997458625 19.98103960504838 56-57 19.862284326026327 29.54084119842405 30.708017997139 19.888856478410627 58-59 19.98657398990252 29.0550246642998 30.952547445561724 20.005853900235955 60-61 19.674741521402527 29.009340192797563 31.47225413316018 19.84366415263973 62-63 19.748583735661292 28.67362137118163 31.64260690535628 19.9351879878008 64-65 19.63883430354822 28.931318376672483 31.4039926831778 20.025854636601494 66-67 19.639954807045864 29.029680103012762 31.05706959055623 20.273295499385142 68-69 19.72556568612075 29.029680103012762 30.899733377553968 20.34502083331252 70-71 19.771852482320515 29.17307263898088 30.768446952555344 20.28662792614326 72-73 19.858955261903834 29.041149537593448 30.5245269786275 20.57536822187522 74-75 19.61550795247595 29.502931542074045 30.161866676523385 20.719693828926612 76-77 19.561167170653103 29.31672625594519 30.307117047728365 20.81498952567334 78-79 19.527531679714194 29.265721969796875 30.13079496001822 21.07595139047071 80-81 19.61484364386123 29.458827149147695 29.927735732951867 20.998593474039208 82-83 19.321942803596777 29.317677099161145 30.00068930586899 21.359690791373087 84-85 19.22055685641448 29.377412471729624 29.692981755120634 21.70904891673526 86-87 19.244792967384246 29.200939014035264 29.558863193646673 21.99540482493382 88-89 19.077264258849503 29.134854077489024 29.613753627459303 22.174128036202166 90-91 18.937890966049512 29.382965418172592 29.634602206092424 22.04454140968547 92-93 19.012356038515595 29.61833037144036 29.23453297490932 22.134780615134726 94-95 18.748164412681987 29.776772602947727 28.912473202922556 22.56258978144773 96-97 18.95461660717907 29.832305911099255 28.683710787384037 22.52936669433764 98-99 19.028695984000734 30.248360215214888 28.123283045933956 22.599660754850426 100-101 18.97230674559807 30.338608524351724 27.89494504704117 22.79413968300904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1076.0 1 784.0 2 502.0 3 551.0 4 540.5 5 571.0 6 875.0 7 1702.0 8 2685.0 9 3102.0 10 2703.0 11 2163.0 12 2129.0 13 2235.5 14 2324.5 15 2444.5 16 2493.0 17 2537.0 18 2611.0 19 2681.5 20 2797.5 21 3074.0 22 3412.5 23 3824.0 24 4290.0 25 4769.5 26 5596.5 27 6548.0 28 7239.0 29 8075.5 30 9046.0 31 9885.5 32 10625.5 33 11427.5 34 12467.5 35 13037.0 36 13987.5 37 14802.0 38 14892.0 39 14768.0 40 14596.0 41 15018.5 42 15403.0 43 15681.5 44 16148.0 45 16376.0 46 16246.5 47 16229.0 48 16985.0 49 16579.0 50 14786.5 51 13638.0 52 12650.0 53 11857.0 54 11095.0 55 10247.5 56 9362.0 57 8299.5 58 7513.0 59 6763.5 60 5771.0 61 4729.0 62 3840.0 63 2919.0 64 1989.0 65 1419.5 66 1021.5 67 746.5 68 541.0 69 354.0 70 242.5 71 186.0 72 142.0 73 111.0 74 86.0 75 63.0 76 49.0 77 32.5 78 18.5 79 13.5 80 14.0 81 12.0 82 6.5 83 4.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023375455771439988 2 0.008191399031017432 3 0.008990559912092303 4 1.9979022026871785E-4 5 0.006792867489136407 6 0.0 7 0.0 8 3.995804405374357E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.005394335947255382 20-21 5.993706608061535E-4 22-23 0.0 24-25 0.0043953848459117924 26-27 0.004994755506717946 28-29 0.00739223814994256 30-31 0.02767094550721742 32-33 1.9979022026871785E-4 34-35 0.0028969581938964087 36-37 0.013685630088407173 38-39 0.005694021277658458 40-41 0.006892762599270766 42-43 0.008291294141151791 44-45 0.002397482643224614 46-47 0.0019979022026871784 48-49 0.0014984266520153837 50-51 0.0022975875330902553 52-53 0.002397482643224614 54-55 0.0015983217621497428 56-57 1.9979022026871785E-4 58-59 7.991608810748714E-4 60-61 0.0 62-63 9.989511013435893E-5 64-65 0.006892762599270766 66-67 8.990559912092302E-4 68-69 8.990559912092302E-4 70-71 0.0030967484141651265 72-73 0.009090455022226662 74-75 0.004095699515508716 76-77 0.0030967484141651265 78-79 0.009390140352629738 80-81 1.9979022026871785E-4 82-83 0.004295489735777434 84-85 1.9979022026871785E-4 86-87 0.0 88-89 4.994755506717946E-4 90-91 3.995804405374357E-4 92-93 9.989511013435893E-5 94-95 7.991608810748714E-4 96-97 9.989511013435893E-5 98-99 3.995804405374357E-4 100-101 0.001198741321612307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 500525.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.61535413290555 #Duplication Level Percentage of deduplicated Percentage of total 1 82.85426322441711 52.70803296442268 2 10.36847380613838 13.191882659904962 3 2.9727338381644617 5.6733454757311135 4 1.2694279642076625 3.2302043795713518 5 0.6382642943465935 2.0301704557623803 6 0.41948342604519384 1.60113520204497 7 0.2806626203434117 1.249811638951075 8 0.19738530602873092 1.0045388914919724 9 0.141472820660463 0.8099859227846751 >10 0.7848822965612244 8.911910357616312 >50 0.048423974912607276 2.153755375398799 >100 0.021382014305712318 2.4053968464553233 >500 9.433241605461285E-4 0.3834632644203613 >1k 0.0015722069342435476 1.8869113058306044 >5k 6.28882773697419E-4 2.7594552596133983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7811 1.5605614105189551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5984 1.1955446780880075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4349 0.868887667948654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.2876979171869537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1363 0.2723140702262624 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1141 0.22796064132660707 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1140 0.22776085110633834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 728 0.1454472803556266 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.12946406273412916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.10808650916537636 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.995804405374357E-4 0.0 10-11 0.0 0.0 0.0 3.995804405374357E-4 0.0 12-13 0.0 0.0 0.0 3.995804405374357E-4 0.0 14-15 0.0 0.0 0.0 4.994755506717946E-4 0.0 16-17 0.0 0.0 0.0 5.993706608061535E-4 0.0 18-19 0.0 0.0 0.0 5.993706608061535E-4 0.0 20-21 0.0 0.0 0.0 5.993706608061535E-4 0.0 22-23 0.0 0.0 0.0 7.991608810748714E-4 0.0 24-25 0.0 0.0 0.0 0.001398531541881025 0.0 26-27 0.0 0.0 0.0 0.0022975875330902553 0.0 28-29 0.0 0.0 0.0 0.0071924479296738425 0.0 30-31 0.0 0.0 0.0 0.020078917137006144 0.0 32-33 0.0 0.0 0.0 0.036461715199041005 0.0 34-35 0.0 0.0 0.0 0.06013685630088407 0.0 36-37 0.0 0.0 0.0 0.09440087907696917 0.0 38-39 0.0 0.0 0.0 0.14704560211777634 0.0 40-41 0.0 0.0 0.0 0.2068827730882573 0.0 42-43 0.0 0.0 0.0 0.2876979171869537 0.0 44-45 0.0 0.0 0.0 0.38289795714499775 0.0 46-47 0.0 0.0 0.0 0.50097397732381 0.0 48-49 0.0 0.0 0.0 0.649318215873333 0.0 50-51 0.0 0.0 0.0 0.822136756405774 0.0 52-53 0.0 0.0 0.0 1.0148344238549523 0.0 54-55 0.0 0.0 0.0 1.1920483492333052 0.0 56-57 0.0 0.0 0.0 1.4456820338644425 0.0 58-59 0.0 0.0 0.0 1.734179111932471 0.0 60-61 0.0 0.0 0.0 2.045352380000999 0.0 62-63 0.0 0.0 0.0 2.4116677488636933 0.0 64-65 0.0 0.0 0.0 2.753608710853604 0.0 66-67 0.0 0.0 0.0 3.1576844313470858 0.0 68-69 0.0 0.0 0.0 3.6240947005644073 0.0 70-71 0.0 0.0 0.0 4.148943609210329 0.0 72-73 0.0 0.0 0.0 4.734928325258478 0.0 74-75 0.0 0.0 0.0 5.298136956195994 0.0 76-77 0.0 0.0 0.0 5.8786274411867545 0.0 78-79 0.0 0.0 0.0 6.553019329703812 0.0 80-81 0.0 0.0 0.0 7.379751261175766 0.0 82-83 0.0 0.0 0.0 8.138354727536086 0.0 84-85 0.0 0.0 0.0 8.992957394735528 0.0 86-87 0.0 0.0 0.0 9.917886219469557 0.0 88-89 0.0 0.0 0.0 10.915338894161131 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3510 0.0 45.75075 1 GTATCAA 5965 0.0 38.948166 1 GTTCCGA 60 0.0039523374 31.660938 6 TATCAAC 7725 0.0 29.942558 2 ATCAACG 7810 0.0 29.677492 3 TCAACGC 7890 0.0 29.313456 4 GGATACG 65 0.0058347187 29.237165 1 CAACGCA 7995 0.0 28.928476 5 AACGCAG 8210 0.0 28.228752 6 AGTCCCG 70 0.008394526 27.137949 5 ATAGTAC 110 9.471364E-5 25.906992 3 ACGCAGA 9155 0.0 25.314919 7 CGCAGAG 9215 0.0 25.201628 8 GCAGAGT 9390 0.0 24.428488 9 GAGTACT 5545 0.0 23.768303 12-13 GTATAGG 180 1.9423351E-7 23.7552 1 AGTACTT 5535 0.0 23.510923 12-13 GTACATG 4405 0.0 22.865389 1 TACATGG 4355 0.0 22.79379 2 GTACTTT 5855 0.0 22.145943 14-15 >>END_MODULE