FastQCFastQC Report
Thu 26 May 2016
SRR936781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527939
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97191.8409323804454683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66601.2615093789244591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.902376979158577No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.3019288213221603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.2295719770655322No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.2057055834102046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.14869142078914419No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.13884179801075502No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.11990021574462201No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.10834585056228087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.055.1738971
GTATCAA57300.045.1147041
GTCGGGA1155.4569682E-1141.3254052
TATCAAC73650.035.038222
ATCAACG74600.034.7194373
TCAACGC75600.034.3230484
GGTCGGG1404.5292836E-1033.9426541
CAACGCA77750.033.3739245
AACGCAG78800.032.9229746
CGGGAGT1456.5847416E-1032.7753224
TTAGGCA1859.094947E-1230.826524
ACGCAGA86600.029.7930267
CGCAGAG86200.029.765918
TTTAGGC1852.8740033E-1028.2576453
TAGGCAT1852.8740033E-1028.2576455
GCAGAGT89550.028.116469
GTGGTAT11550.027.565551
TCGGGAG1754.9221853E-927.1566963
CATTCCG1754.942194E-927.1464049
GTAGCAC1109.434355E-525.92233
Produced by FastQC (version 0.11.5)