##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527939 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.061277912789166 33.0 31.0 34.0 31.0 34.0 2 32.14785041453653 33.0 31.0 34.0 30.0 34.0 3 32.10359719588816 34.0 31.0 34.0 30.0 34.0 4 35.727462831880196 37.0 35.0 37.0 35.0 37.0 5 35.73859479977801 37.0 35.0 37.0 35.0 37.0 6 35.876103110397224 37.0 35.0 37.0 35.0 37.0 7 35.83074370334452 37.0 35.0 37.0 35.0 37.0 8 35.80341100013449 37.0 35.0 37.0 35.0 37.0 9 37.440933516940405 39.0 37.0 39.0 35.0 39.0 10-11 37.35124417783115 39.0 37.0 39.0 34.5 39.0 12-13 37.25069562960872 39.0 37.0 39.0 34.0 39.0 14-15 38.36362913139587 40.0 38.0 41.0 34.0 41.0 16-17 38.34294302940302 40.0 38.0 41.0 34.0 41.0 18-19 38.43756002113881 40.0 38.0 41.0 34.0 41.0 20-21 38.427573071888986 40.0 38.0 41.0 34.0 41.0 22-23 38.287906367970535 40.0 38.0 41.0 34.0 41.0 24-25 38.350505456122775 40.0 38.0 41.0 34.0 41.0 26-27 38.271635548803935 40.0 38.0 41.0 34.0 41.0 28-29 38.151572435451826 40.0 38.0 41.0 34.0 41.0 30-31 37.83005991222471 40.0 37.0 41.0 33.5 41.0 32-33 37.457952528606526 40.0 36.0 41.0 33.0 41.0 34-35 37.201777099248204 39.5 35.0 41.0 33.0 41.0 36-37 36.972525234922976 39.0 35.0 41.0 32.5 41.0 38-39 36.77331377299271 39.0 35.0 41.0 31.5 41.0 40-41 36.5162206239736 39.0 35.0 41.0 31.0 41.0 42-43 36.15532002750318 39.0 35.0 41.0 30.5 41.0 44-45 35.651710709002366 39.0 35.0 41.0 29.0 41.0 46-47 34.95813247363805 39.0 35.0 40.0 25.0 41.0 48-49 34.38930823447406 38.5 35.0 40.0 20.5 41.0 50-51 33.54585567650808 38.0 34.0 40.0 14.5 40.5 52-53 33.6911290887773 38.0 34.0 40.0 13.5 40.5 54-55 34.033242476877064 39.0 35.0 40.5 9.0 41.0 56-57 33.92234614226265 39.0 35.0 41.0 7.5 41.0 58-59 33.68102375463832 38.0 34.0 40.5 4.0 41.0 60-61 33.42782688909135 38.0 34.0 40.0 2.0 41.0 62-63 33.09973690142232 38.0 34.0 40.0 2.0 41.0 64-65 32.74931194702418 37.0 33.0 40.0 2.0 41.0 66-67 32.379791983543555 36.5 33.0 40.0 2.0 41.0 68-69 31.97493081587077 36.0 33.0 39.0 2.0 41.0 70-71 31.572908044300576 35.5 32.0 39.0 2.0 41.0 72-73 31.17033691392377 35.0 32.0 38.5 2.0 40.0 74-75 30.736663705465972 35.0 31.0 37.5 2.0 40.0 76-77 30.304844877912032 35.0 31.0 37.0 2.0 39.0 78-79 29.893351315208765 35.0 31.0 36.5 2.0 39.0 80-81 29.462308145448624 35.0 30.0 36.0 2.0 38.5 82-83 29.05044901020762 35.0 29.5 36.0 2.0 37.0 84-85 28.664278448835944 34.0 29.0 35.0 2.0 37.0 86-87 28.303059633783448 34.0 29.0 35.0 2.0 36.5 88-89 27.93303677129365 34.0 29.0 35.0 2.0 36.0 90-91 27.536476752049005 34.0 27.0 35.0 2.0 36.0 92-93 27.208845150670818 34.0 27.0 35.0 2.0 35.0 94-95 26.85085492831558 34.0 25.5 35.0 2.0 35.0 96-97 26.370927323043002 34.0 25.0 35.0 2.0 35.0 98-99 25.79122303902534 34.0 22.0 35.0 2.0 35.0 100-101 23.615625100627156 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38567588261691554 1101 2 0.4123082324788925 1101 3 0.31920378131265537 1101 4 0.43540097311220194 1101 5 0.3158191502731569 1101 6 0.15625110971413392 1101 7 0.21446036898591814 1101 8 0.1587468444017972 1101 9 0.29632602926492524 1101 10-11 0.3427044064914 1101 12-13 0.40232840769999 1101 14-15 0.4799367796416618 1101 16-17 0.555699299424532 1101 18-19 0.6416910444342676 1101 20-21 0.5150174788684012 1101 22-23 0.5441342518501457 1101 24-25 0.5069666098314656 1101 26-27 0.523264010330827 1101 28-29 0.34083669511454673 1101 30-31 0.4599033507091832 1101 32-33 0.6585255714726372 1101 34-35 0.6748213610874885 1101 36-37 0.5156029395585122 1101 38-39 0.6575642192734534 1101 40-41 0.5060000394608437 1101 42-43 0.49835727104319005 1101 44-45 0.5252848848835399 1101 46-47 0.6175013372022065 1101 48-49 0.7598290511136199 1101 50-51 0.7978725360407921 1101 52-53 0.8870684317486237 1101 54-55 0.996998463346003 1101 56-57 0.7429580708697117 1101 58-59 0.7061023934366801 1101 60-61 0.8494383332380337 1101 62-63 0.999895033756836 1101 64-65 1.006823152082994 1101 66-67 0.9438422397250434 1101 68-69 0.8743555920908825 1101 70-71 0.8334023122486904 1101 72-73 0.7872839602533048 1101 74-75 0.8535469523946269 1101 76-77 0.9178431073313895 1101 78-79 0.861319159743946 1101 80-81 0.8156835562064089 1101 82-83 0.934066563797451 1101 84-85 0.8092666878101973 1101 86-87 0.7452709161221591 1101 88-89 0.9206364639333806 1101 90-91 0.8043748535128579 1101 92-93 0.7636277420608657 1101 94-95 0.9199879514109135 1101 96-97 0.8035647848249461 1101 98-99 0.6416498121043404 1101 100-101 0.8010635654797476 1102 1 0.25623882252993013 1102 2 0.3573879002423084 1102 3 0.23708470210302934 1102 4 0.14331211886434403 1102 5 0.2670419820188883 1102 6 0.26654172748141747 1102 7 0.3045945666262284 1102 8 0.1929142895000595 1102 9 0.2626568795502209 1102 10-11 0.29472488271304087 1102 12-13 0.1819085603717454 1102 14-15 0.2578156626270314 1102 16-17 0.35185409525729483 1102 18-19 0.4163046052084596 1102 20-21 0.4393888829606709 1102 22-23 0.3008906166440113 1102 24-25 0.17662391647798614 1102 26-27 0.3503737334868333 1102 28-29 0.3571744620372641 1102 30-31 0.22839382806129294 1102 32-33 0.4753930933232553 1102 34-35 0.38951043964264187 1102 36-37 0.27018130729157974 1102 38-39 0.4409697186695496 1102 40-41 0.5746253540562876 1102 42-43 0.6562726560247185 1102 44-45 0.3973917967611129 1102 46-47 0.58580826335929 1102 48-49 0.7905556550297774 1102 50-51 0.5891087102032131 1102 52-53 0.9029748384885536 1102 54-55 1.0488457261425168 1102 56-57 0.9620823502007099 1102 58-59 1.1020099752612467 1102 60-61 0.9619448780132203 1102 62-63 1.0961526866814566 1102 64-65 1.1085501546354593 1102 66-67 1.0814624999691063 1102 68-69 1.1146559377740068 1102 70-71 1.158871751123467 1102 72-73 0.943555342331333 1102 74-75 0.9072438579059217 1102 76-77 1.0511252228407209 1102 78-79 0.8764788671329953 1102 80-81 0.9466177336174155 1102 82-83 1.1816859018231902 1102 84-85 1.0621199866743787 1102 86-87 0.9010625959279572 1102 88-89 1.010289471852559 1102 90-91 1.0839133576152413 1102 92-93 1.1612194879093956 1102 94-95 1.2064047911174711 1102 96-97 1.1799626149344817 1102 98-99 1.189629707744924 1102 100-101 1.4634390660063055 1103 1 0.05952413813363577 1103 2 0.37507443065353385 1103 3 0.3160307744989943 1103 4 0.3287959898320878 1103 5 0.3220428028347797 1103 6 0.23026782121858957 1103 7 0.17861779571594383 1103 8 0.377731247556369 1103 9 0.44190049770666917 1103 10-11 0.276427870482884 1103 12-13 0.37490905286127685 1103 14-15 0.6042933364348002 1103 16-17 0.5972718905458052 1103 18-19 0.59749355702656 1103 20-21 0.5717351851850836 1103 22-23 0.4314711386829231 1103 24-25 0.34789638631300335 1103 26-27 0.4539699331092564 1103 28-29 0.561925960026258 1103 30-31 0.5974162363351141 1103 32-33 0.5163990031976766 1103 34-35 0.3832424842950317 1103 36-37 0.6339751183397624 1103 38-39 0.8025729773086283 1103 40-41 0.7380179913377489 1103 42-43 0.6590726642328804 1103 44-45 0.7722522580596447 1103 46-47 0.9694658189695673 1103 48-49 1.1121974920157456 1103 50-51 0.8611055500620282 1103 52-53 1.0882442713047666 1103 54-55 1.0572939737005953 1103 56-57 1.0780082305257537 1103 58-59 1.1941034661912298 1103 60-61 1.30268299670319 1103 62-63 1.2696659838988893 1103 64-65 1.1961404443834667 1103 66-67 1.0825623932630464 1103 68-69 1.3420342518181663 1103 70-71 1.2235623035325638 1103 72-73 1.1981929521154555 1103 74-75 1.2424732759474573 1103 76-77 1.2058797988893168 1103 78-79 0.9806270654421176 1103 80-81 1.2105305629697938 1103 82-83 1.246696572429773 1103 84-85 1.1471370186041163 1103 86-87 1.16695564361736 1103 88-89 1.2054138254600453 1103 90-91 1.180012430093285 1103 92-93 1.3173622688336302 1103 94-95 1.4085337823347395 1103 96-97 1.0035538896615606 1103 98-99 1.125357771440239 1103 100-101 1.3853456161171138 1104 1 0.06578768666429369 1104 2 0.06040540959319429 1104 3 0.19081435690995008 1104 4 0.17969396132095028 1104 5 0.08801320064117135 1104 6 0.08399680753726813 1104 7 0.017903151100185255 1104 8 0.10598540722445193 1104 9 0.07521229695153409 1104 10-11 0.24006612650883596 1104 12-13 0.23677433560386163 1104 14-15 0.26653403011956556 1104 16-17 0.2972991670578651 1104 18-19 0.18665616810657326 1104 20-21 0.1543255924664777 1104 22-23 0.2006838524087442 1104 24-25 0.21540144682379037 1104 26-27 0.07419187778100422 1104 28-29 -0.03527819748842376 1104 30-31 -0.19602448031935893 1104 32-33 0.13127905832494235 1104 34-35 0.22296713508389843 1104 36-37 0.2577652614696717 1104 38-39 0.16042071694715077 1104 40-41 0.1919070477139897 1104 42-43 0.2653118074632772 1104 44-45 0.17357301548531723 1104 46-47 0.2804876832473866 1104 48-49 0.2340050634012485 1104 50-51 0.09201270942863715 1104 52-53 0.20774697742188408 1104 54-55 -0.019355896377071247 1104 56-57 0.4476903873800566 1104 58-59 0.5546070767400622 1104 60-61 0.5502948662927523 1104 62-63 0.6330192721542005 1104 64-65 0.6956745902697392 1104 66-67 0.5577285646456716 1104 68-69 0.6685649666063433 1104 70-71 0.6420414226447839 1104 72-73 0.8298057117806721 1104 74-75 0.7616400502845657 1104 76-77 0.5330005477013522 1104 78-79 0.7822966751008735 1104 80-81 0.7068374667985537 1104 82-83 0.691094120414899 1104 84-85 0.42934053009614104 1104 86-87 0.37425713521188797 1104 88-89 0.3601427112863078 1104 90-91 0.2098357070073078 1104 92-93 0.2023321928900792 1104 94-95 0.5270301287177297 1104 96-97 0.33943542346289846 1104 98-99 0.6941713867265698 1104 100-101 0.7619789767269616 1105 1 0.30460783173744943 1105 2 0.4272083404196394 1105 3 0.40802722722041906 1105 4 0.3520750054622823 1105 5 0.41877265540101405 1105 6 0.21996962811360987 1105 7 0.13813447901048193 1105 8 0.22209440020019855 1105 9 0.23042726857464402 1105 10-11 0.2917753929305036 1105 12-13 0.29408098501957625 1105 14-15 0.08482210264696732 1105 16-17 0.3370623794051397 1105 18-19 0.4685887792857386 1105 20-21 0.4619912543570095 1105 22-23 0.1170270637466615 1105 24-25 -0.20180431205746885 1105 26-27 0.08376707311590792 1105 28-29 -0.21685133739087803 1105 30-31 -0.32433301541081505 1105 32-33 0.10797421658865147 1105 34-35 -0.39002544608963063 1105 36-37 -0.06037140643576322 1105 38-39 -0.11946449082128652 1105 40-41 -0.04627421573464119 1105 42-43 0.1653801689441039 1105 44-45 0.0245690743771263 1105 46-47 -0.22069602561528967 1105 48-49 -0.23776370962487192 1105 50-51 -0.16212912458601636 1105 52-53 -0.2326902148940988 1105 54-55 0.013219037440755699 1105 56-57 0.13017539602846995 1105 58-59 0.28074020269494326 1105 60-61 0.3004049787450995 1105 62-63 0.32991983602171615 1105 64-65 0.3515445015833407 1105 66-67 0.22272978047067227 1105 68-69 0.636369691348154 1105 70-71 0.6634525596017333 1105 72-73 0.4108633877656054 1105 74-75 0.5545839066979283 1105 76-77 0.6578806857089567 1105 78-79 0.8074699875128388 1105 80-81 0.8554147221539168 1105 82-83 0.6404389513234641 1105 84-85 0.660810668975472 1105 86-87 0.8693445140308391 1105 88-89 0.8606737744679158 1105 90-91 0.9600479028790083 1105 92-93 0.6774720127644613 1105 94-95 0.8610390478263703 1105 96-97 0.8234186496659923 1105 98-99 0.6381390595457717 1105 100-101 0.689352932379169 1106 1 -0.0513629586405564 1106 2 0.12057174248149494 1106 3 0.024028086326946863 1106 4 0.17664545219767547 1106 5 0.15315839423262645 1106 6 0.053453305089554703 1106 7 0.20731403227508594 1106 8 0.01812103250260577 1106 9 0.049091357921717815 1106 10-11 -0.07639471016227617 1106 12-13 0.08045340769998433 1106 14-15 0.17690758374661897 1106 16-17 0.10385791205118977 1106 18-19 -0.020113969855152902 1106 20-21 0.07606986260443449 1106 22-23 0.004118988145293656 1106 24-25 0.14994611749579434 1106 26-27 0.1078005782705489 1106 28-29 0.07657649766066754 1106 30-31 0.19499688149640093 1106 32-33 0.18918126126219192 1106 34-35 0.02941183913373635 1106 36-37 -0.10496977413335884 1106 38-39 -0.11829395817524357 1106 40-41 -0.09148469683429283 1106 42-43 0.23077965348019092 1106 44-45 0.4971850537585638 1106 46-47 0.5194994211201092 1106 48-49 0.42459668556413277 1106 50-51 0.227873023180301 1106 52-53 0.5191246476488587 1106 54-55 0.4749351027328572 1106 56-57 0.6045203272999444 1106 58-59 0.29296099307294554 1106 60-61 0.32660772788598535 1106 62-63 0.40998856454405086 1106 64-65 0.5959388314802467 1106 66-67 0.7118298663813221 1106 68-69 0.6005019937536566 1106 70-71 0.3669426798766473 1106 72-73 0.7335760166315879 1106 74-75 0.9736560716463849 1106 76-77 0.9450263042656566 1106 78-79 1.0024012589905027 1106 80-81 0.8531380898515124 1106 82-83 0.9136992606018133 1106 84-85 0.9224730401094945 1106 86-87 0.9988440844775788 1106 88-89 0.6548425889009053 1106 90-91 0.47816431181371755 1106 92-93 0.44481522582288235 1106 94-95 0.27761980384011764 1106 96-97 0.5321493197690863 1106 98-99 0.26030400799938036 1106 100-101 0.19085636880528867 1107 1 -0.1760594266317277 1107 2 0.050635208265155995 1107 3 0.001083825841295294 1107 4 -0.12545167804514534 1107 5 0.19014790858141595 1107 6 -0.006163140826558333 1107 7 -0.20663464322160507 1107 8 -0.005733824009539035 1107 9 -0.09399639042265306 1107 10-11 -0.1544024364315959 1107 12-13 -0.0424839543309119 1107 14-15 0.07629362127421757 1107 16-17 0.1150885963778947 1107 18-19 -0.013187921290032989 1107 20-21 -0.13370524777084114 1107 22-23 -0.1439080537973183 1107 24-25 0.02849882169005724 1107 26-27 -0.1082176336499785 1107 28-29 0.11208974269378302 1107 30-31 -0.10053291982810464 1107 32-33 -0.07907204999609263 1107 34-35 -0.33381939706935526 1107 36-37 -0.424513096429159 1107 38-39 -0.5042418058573617 1107 40-41 -0.43399297497999356 1107 42-43 -0.3293058873586645 1107 44-45 0.06568670938770538 1107 46-47 0.003764045844164343 1107 48-49 -0.11497974931445754 1107 50-51 -0.016684096024995654 1107 52-53 -0.18208258193171645 1107 54-55 -0.04331131007067768 1107 56-57 0.04041298734409793 1107 58-59 -0.46872498926700956 1107 60-61 -0.15600816615374669 1107 62-63 0.008995187060605758 1107 64-65 0.15006410741843723 1107 66-67 -0.048759261653998465 1107 68-69 -0.14715253163265984 1107 70-71 0.03866591387223295 1107 72-73 0.11510885327617615 1107 74-75 0.0771880804764038 1107 76-77 0.054042584618859024 1107 78-79 0.04119816848277935 1107 80-81 -0.14688950396747202 1107 82-83 -0.04586199767632948 1107 84-85 0.023342245407508955 1107 86-87 -0.1037842267807001 1107 88-89 -0.21407987246774596 1107 90-91 -0.3137851914975407 1107 92-93 -0.6773219154574726 1107 94-95 -0.45510646547555567 1107 96-97 -0.2052334065002306 1107 98-99 -0.3851706056871578 1107 100-101 -0.06866349874438171 1108 1 -0.30447477298654846 1108 2 0.10145254416925553 1108 3 -0.1715151626181992 1108 4 0.14014756190231736 1108 5 0.24263624233389436 1108 6 0.14519908568027518 1108 7 -0.5060562208894694 1108 8 0.02716639115239161 1108 9 -0.20140969693060384 1108 10-11 0.09602090165206789 1108 12-13 0.03903726845948796 1108 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417.0 8 490.0 9 652.0 10 859.0 11 1291.0 12 2092.0 13 2964.0 14 3580.0 15 5049.0 16 7282.0 17 11535.0 18 9999.0 19 4479.0 20 3811.0 21 3717.0 22 3806.0 23 3921.0 24 4127.0 25 4539.0 26 5017.0 27 5520.0 28 6462.0 29 7916.0 30 9638.0 31 12100.0 32 15807.0 33 20918.0 34 30371.0 35 46307.0 36 66801.0 37 101713.0 38 101343.0 39 20903.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49183433746353 19.485809556288128 15.907695805388201 19.11466030086014 2 14.93611418937478 21.457676242434577 42.44935072315517 21.15685884503547 3 17.93771584345418 29.0582358189785 32.041161571671864 20.962886765895455 4 11.301500930266045 18.138861627188778 39.582157080442435 30.977480362102742 5 11.331128526348706 38.68545490727492 34.18253148483327 15.800885081543111 6 26.53000121278273 39.178567097204535 18.542310957491964 15.749120732520769 7 24.32379692973399 37.28253229690227 21.656875133800778 16.73679563956297 8 25.876478168587024 33.42177077016373 20.335809581564586 20.365941479684658 9 27.845988275373358 16.09161427721504 21.5063909446775 34.55600650273411 10-11 23.36588304838123 28.410804686552687 27.09252228082939 21.130789984236692 12-13 26.148416589687105 26.886662403183404 28.285369146592764 18.679551860536726 14-15 22.832537770675234 26.224486026105314 29.714857333938333 21.228118869281115 16-17 18.6643856702653 30.401476206308924 29.47373207196506 21.46040605146072 18-19 18.668111516106787 26.485738426680207 35.78967210505975 19.056477952153255 20-21 20.703033609632683 25.13376885764379 35.87846996274647 18.28472756997705 22-23 19.936351883160242 25.75882857156388 35.817598893735116 18.487220651540767 24-25 19.086264897751985 25.733996044945183 36.34720001818416 18.83253903911867 26-27 18.816414048619347 25.983448814065284 36.31146081547455 18.888676321840823 28-29 18.700253444611555 26.10432143898955 36.261710921606436 18.933714194792454 30-31 18.976245361680043 25.82987807303495 36.320957534866714 18.872919030418288 32-33 18.66967585270268 26.236553844288828 35.98389207844088 19.109878224567613 34-35 18.877749133896153 26.23996332909673 35.88312286078506 18.999164676222062 36-37 19.133460494488947 25.949778288779573 35.808114195011164 19.108647021720312 38-39 18.891801688834676 26.369289575669868 35.52083767412083 19.21807106137463 40-41 18.87509731237889 26.552025896931276 35.43970041993488 19.133176370754953 42-43 18.83988381222434 26.68075921721943 34.92262829856129 19.55672867199494 44-45 19.01280261545368 26.79182632842052 34.63155781255031 19.56381324357549 46-47 19.32050861936701 27.214223612955283 33.73903045617013 19.72623731150758 48-49 19.394720018941637 27.657440511424173 32.95200662957263 19.99583284006156 50-51 19.45868746199845 28.071803750054457 32.3272196219639 20.14228916598319 52-53 19.57462848418897 28.453314164433923 31.851531949270605 20.120525402106495 54-55 19.330327250134722 28.317038543972256 31.89905091494224 20.45358329095078 56-57 19.44783288946808 28.45504585765753 31.606646992639288 20.490474260235104 58-59 19.54486950608643 27.976470655088253 31.937458387514404 20.541201451310904 60-61 19.500497229720132 27.93502864990292 32.30288393237676 20.261590188000188 62-63 19.522476831791945 27.709792445056898 32.50548128785403 20.262249435297125 64-65 19.458398208867017 27.802149511113132 32.43350494569397 20.305947334325882 66-67 19.35397100219359 27.962860530931877 32.049698618304156 20.63346984857038 68-69 19.448596201095306 27.861633790437008 32.15713909552955 20.53263091293814 70-71 19.479659547863648 27.973627747701663 31.89754228417428 20.649170420260408 72-73 19.56215137442186 27.969136720426086 31.607203578867406 20.861508326284653 74-75 19.356565063367874 28.3785780589918 31.42752950537064 20.837327372269687 76-77 19.278579629636646 28.16688328666214 31.40462594210752 21.14991114159369 78-79 19.351810629813112 28.048422388724177 31.31863864129354 21.281128340169175 80-81 19.193933800887226 28.237899153134954 31.38611853836664 21.182048507611174 82-83 19.21536342328994 28.342203939951453 31.082207691564367 21.36022494519424 84-85 19.175258708470484 28.0430983447394 30.90804897108622 21.8735939757039 86-87 19.239009568263146 28.014779566236214 30.79622497826256 21.949985887238086 88-89 19.277020563190845 27.961014236055203 30.80538374835428 21.95658145239967 90-91 19.126331124974662 27.954050730993874 30.978908501202064 21.9407096428294 92-93 19.191102008414102 28.18818331408694 30.64013285222085 21.980581825278108 94-95 19.162591484408225 28.205186492274443 30.460010172564196 22.17221185075314 96-97 19.272196473041035 28.254508632685326 30.186786549549726 22.286508344723913 98-99 19.49276021357465 28.711787910826143 29.563850709957595 22.231601165641614 100-101 19.771281307884355 28.656443490660337 29.23364078658765 22.338634414867663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 574.5 2 492.5 3 613.5 4 493.0 5 443.0 6 827.0 7 1991.5 8 3619.0 9 4135.0 10 3161.0 11 2286.0 12 2151.5 13 2222.0 14 2325.5 15 2367.5 16 2392.5 17 2448.5 18 2559.0 19 2661.0 20 2755.0 21 3035.0 22 3389.5 23 3755.5 24 4227.0 25 4892.0 26 5827.0 27 6738.0 28 7519.5 29 8360.5 30 9228.0 31 10257.0 32 11341.5 33 12107.0 34 13064.5 35 13828.5 36 14796.5 37 15999.5 38 16094.5 39 15701.5 40 15745.5 41 15943.5 42 15989.0 43 16443.5 44 17036.0 45 17394.0 46 17273.5 47 17145.5 48 17868.0 49 17408.5 50 15617.0 51 14379.5 52 13464.0 53 12743.5 54 11983.5 55 11099.0 56 9980.0 57 8593.5 58 7779.5 59 7056.5 60 6076.0 61 5140.5 62 4118.5 63 3081.0 64 2127.5 65 1513.0 66 1136.5 67 853.0 68 591.5 69 360.5 70 230.0 71 173.0 72 133.5 73 101.5 74 74.0 75 64.5 76 59.5 77 32.0 78 14.5 79 8.5 80 6.5 81 7.0 82 5.5 83 3.5 84 3.5 85 4.0 86 3.0 87 1.5 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022540482896698293 2 0.008334296197098528 3 0.012880275940970451 4 0.025192304413956916 5 0.05758241008904438 6 0.042997391744121954 7 0.01932041391145568 8 0.04905869806928452 9 0.03049594744847416 10-11 0.024813472768634257 12-13 0.03816728826625804 14-15 0.021877527517383637 16-17 0.018846874354802354 18-19 0.016479176571535727 20-21 0.013164399674962449 22-23 0.0069136775271385524 24-25 0.0013259107586293113 26-27 1.8941582266133019E-4 28-29 0.0024624056945972924 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.8941582266133019E-4 40-41 0.0 42-43 9.470791133066509E-5 44-45 0.0 46-47 0.0 48-49 2.841237339919953E-4 50-51 0.0 52-53 9.470791133066509E-5 54-55 0.0017994503152826368 56-57 1.8941582266133019E-4 58-59 4.7353955665332547E-4 60-61 0.0026518215172586226 62-63 0.0012312028472986462 64-65 9.470791133066509E-4 66-67 0.007008385438469217 68-69 0.009470791133066508 70-71 0.021119864226738318 72-73 0.011838488916333137 74-75 0.013069691763631784 76-77 0.025192304413956916 78-79 0.015910929103551734 80-81 0.04318680756678329 82-83 0.03144302656178081 84-85 0.01449031043359176 86-87 0.009091959487743849 88-89 0.010228454423711831 90-91 0.018183918975487698 92-93 0.025476428147948912 94-95 0.009186667399074514 96-97 0.017142131950850382 98-99 0.03049594744847416 100-101 0.04100852560617799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527939.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.98135195462329 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87675790852454 53.025670166034175 2 10.439678942587753 13.358895454379526 3 2.955207480962139 5.672345098151229 4 1.2633739396794843 3.2332949073972843 5 0.6400876738013257 2.0476837369649363 6 0.4010171992936903 1.5394573540720142 7 0.2635898273163405 1.1805383459229613 8 0.1842112888502087 0.9428869844751981 9 0.15467248772571243 0.890653938738836 >10 0.7587510731981069 8.775410830507841 >50 0.0407830226909337 1.7930792324323497 >100 0.018913864103541605 2.2915033077023024 >500 0.0011821165064713082 0.5148744539048806 >1k 0.0011821165064713082 1.6367070411313431 >5k 5.910582532356541E-4 3.096999148185105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9719 1.8409323804454683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6660 1.2615093789244591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4764 0.902376979158577 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 0.3019288213221603 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 0.2295719770655322 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1086 0.2057055834102046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 785 0.14869142078914419 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 733 0.13884179801075502 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.11990021574462201 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 572 0.10834585056228087 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8941582266133019E-4 0.0 22-23 0.0 0.0 0.0 4.7353955665332547E-4 0.0 24-25 0.0 0.0 0.0 0.0017994503152826368 0.0 26-27 0.0 0.0 0.0 0.002935945251250618 0.0 28-29 0.0 0.0 0.0 0.007860756640445204 0.0 30-31 0.0 0.0 0.0 0.017331547773511712 0.0 32-33 0.0 0.0 0.0 0.03030653162581283 0.0 34-35 0.0 0.0 0.0 0.049153405980615184 0.0 36-37 0.0 0.0 0.0 0.07500866577388676 0.0 38-39 0.0 0.0 0.0 0.1128918303061528 0.0 40-41 0.0 0.0 0.0 0.15787808818821872 0.0 42-43 0.0 0.0 0.0 0.22900372959754822 0.0 44-45 0.0 0.0 0.0 0.3169873792237361 0.0 46-47 0.0 0.0 0.0 0.42012429466283036 0.0 48-49 0.0 0.0 0.0 0.541823960722735 0.0 50-51 0.0 0.0 0.0 0.6847381989207086 0.0 52-53 0.0 0.0 0.0 0.8500035041927192 0.0 54-55 0.0 0.0 0.0 1.0018202860557754 0.0 56-57 0.0 0.0 0.0 1.2146289628157798 0.0 58-59 0.0 0.0 0.0 1.4640896012607518 0.0 60-61 0.0 0.0 0.0 1.7362801384250832 0.0 62-63 0.0 0.0 0.0 2.0536463492941417 0.0 64-65 0.0 0.0 0.0 2.355954002261625 0.0 66-67 0.0 0.0 0.0 2.7101615906383123 0.0 68-69 0.0 0.0 0.0 3.1289599745425134 0.0 70-71 0.0 0.0 0.0 3.613769772644188 0.0 72-73 0.0 0.0 0.0 4.1746300235443865 0.0 74-75 0.0 0.0 0.0 4.720147592809018 0.0 76-77 0.0 0.0 0.0 5.27163176048748 0.0 78-79 0.0 0.0 0.0 5.916497928737979 0.0 80-81 0.0 0.0 0.0 6.696512286457336 0.0 82-83 0.0 0.0 0.0 7.4153453334570845 0.0 84-85 0.0 0.0 0.0 8.238262375009235 0.0 86-87 0.0 0.0 0.0 9.148878942453578 0.0 88-89 0.0 0.0 0.0 10.10590238644995 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2980 0.0 55.173897 1 GTATCAA 5730 0.0 45.114704 1 GTCGGGA 115 5.4569682E-11 41.325405 2 TATCAAC 7365 0.0 35.03822 2 ATCAACG 7460 0.0 34.719437 3 TCAACGC 7560 0.0 34.323048 4 GGTCGGG 140 4.5292836E-10 33.942654 1 CAACGCA 7775 0.0 33.373924 5 AACGCAG 7880 0.0 32.922974 6 CGGGAGT 145 6.5847416E-10 32.775322 4 TTAGGCA 185 9.094947E-12 30.82652 4 ACGCAGA 8660 0.0 29.793026 7 CGCAGAG 8620 0.0 29.76591 8 TTTAGGC 185 2.8740033E-10 28.257645 3 TAGGCAT 185 2.8740033E-10 28.257645 5 GCAGAGT 8955 0.0 28.11646 9 GTGGTAT 1155 0.0 27.56555 1 TCGGGAG 175 4.9221853E-9 27.156696 3 CATTCCG 175 4.942194E-9 27.146404 9 GTAGCAC 110 9.434355E-5 25.9223 3 >>END_MODULE