FastQCFastQC Report
Thu 26 May 2016
SRR936781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527939
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80351.521956135083788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61251.1601719138006474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.842710995020258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.2727587846323155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14030.26575039919384624No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.23506503592271077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.21953293846448171No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7510.14225128281865898No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.1142177410647821No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.11024000878889417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.049.7602921
GTATCAA58750.045.2076871
TATCAAC76950.034.5087552
ATCAACG77050.034.3990553
TCAACGC77550.034.174034
CAACGCA79150.033.426375
AACGCAG81850.032.3817636
CGCAGAG92650.028.1969538
ACGCAGA93150.028.1475877
GCAGAGT94700.026.8843659
CATGGGT3650.026.0244124
GAGTACT57800.024.81549512-13
GTATAGT1351.3381954E-524.6361391
GTACATG46650.024.4437581
TACATGG47350.023.9775072
AGTACTT58500.023.86905912-13
CTAAGGG1601.8551764E-623.7517782
ACAGTAC1852.5367262E-723.1076478
GTACTTT61500.023.05223714-15
ACATGGG48900.022.3410683
Produced by FastQC (version 0.11.5)