##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527939 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85759339620676 31.0 31.0 34.0 30.0 34.0 2 31.967931901223437 31.0 31.0 34.0 30.0 34.0 3 32.01918024620269 33.0 31.0 34.0 30.0 34.0 4 35.80997236423148 37.0 35.0 37.0 35.0 37.0 5 35.71599559797628 37.0 35.0 37.0 33.0 37.0 6 35.78668179467704 37.0 35.0 37.0 35.0 37.0 7 35.718022347278755 37.0 35.0 37.0 33.0 37.0 8 35.694146482832295 37.0 35.0 37.0 33.0 37.0 9 37.26081801117174 39.0 37.0 39.0 34.0 39.0 10-11 37.18299841458956 39.0 37.0 39.0 33.0 39.0 12-13 37.05818569948422 39.0 37.0 39.0 33.0 39.0 14-15 38.044585643417136 40.0 37.0 41.0 33.0 41.0 16-17 37.98736501754938 40.0 37.0 41.0 33.0 41.0 18-19 38.24985651751433 40.0 37.5 41.0 33.5 41.0 20-21 38.33596116217972 40.0 38.0 41.0 34.0 41.0 22-23 38.22888345055016 40.0 37.5 41.0 33.5 41.0 24-25 38.08505149269139 40.0 37.0 41.0 33.0 41.0 26-27 37.84222514343513 40.0 37.0 41.0 33.0 41.0 28-29 37.669293232740905 39.5 37.0 41.0 33.0 41.0 30-31 37.497714698099585 39.0 36.5 41.0 33.0 41.0 32-33 37.15257823347015 39.0 35.0 41.0 32.0 41.0 34-35 36.89447265687892 39.0 35.0 41.0 31.0 41.0 36-37 36.630468671570014 39.0 35.0 40.5 31.0 41.0 38-39 36.39008105103052 39.0 35.0 40.0 30.5 41.0 40-41 36.101737132509626 38.0 35.0 40.0 30.0 41.0 42-43 35.68185907841625 38.0 35.0 40.0 29.5 41.0 44-45 35.14175501336329 38.0 35.0 40.0 26.5 41.0 46-47 34.41355630101205 38.0 34.0 40.0 23.5 41.0 48-49 33.73626403808015 38.0 33.0 40.0 18.0 41.0 50-51 33.314380070424804 38.0 33.0 40.0 12.0 41.0 52-53 32.99582622234766 38.0 33.0 40.0 8.5 41.0 54-55 32.63432328356117 37.0 32.5 40.0 7.0 41.0 56-57 32.298615938583815 37.0 32.0 40.0 2.0 41.0 58-59 31.916333136972263 36.5 31.0 39.5 2.0 41.0 60-61 31.539540552980554 36.0 31.0 39.0 2.0 40.0 62-63 31.147894927254853 35.5 30.5 39.0 2.0 40.0 64-65 30.729541670533905 35.0 29.5 39.0 2.0 40.0 66-67 30.289586486317546 35.0 29.0 38.0 2.0 40.0 68-69 29.972101890559326 35.0 29.0 38.0 2.0 40.0 70-71 29.48020225821544 34.0 28.5 37.0 2.0 39.5 72-73 28.934160007122035 34.0 27.5 36.5 2.0 39.0 74-75 28.391893760453385 34.0 26.0 36.0 2.0 39.0 76-77 27.10467591899822 31.5 25.5 34.5 2.0 37.0 78-79 27.79274120684397 33.5 26.0 35.0 2.0 37.0 80-81 27.488811207355397 33.0 26.0 35.0 2.0 37.0 82-83 27.136011925620195 33.0 25.5 35.0 2.0 36.5 84-85 26.756191529703244 33.0 25.0 35.0 2.0 36.0 86-87 26.365783736378635 33.0 24.5 35.0 2.0 36.0 88-89 25.903574087157796 32.0 23.5 35.0 2.0 35.0 90-91 25.398126488098058 32.0 20.5 34.0 2.0 35.0 92-93 24.810898607604287 32.0 18.5 34.0 2.0 35.0 94-95 24.093042946249472 31.0 8.5 34.0 2.0 35.0 96-97 23.26340353715107 31.0 2.0 34.0 2.0 35.0 98-99 22.308350964789494 30.5 2.0 34.0 2.0 35.0 100-101 19.05930230575881 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2618675240082453 1101 2 0.21578949133343883 1101 3 0.3279503661616552 1101 4 0.33534572368543536 1101 5 0.33943650093909383 1101 6 0.24292266941111507 1101 7 0.3127127889700674 1101 8 0.28632500393845106 1101 9 0.410385448127947 1101 10-11 0.43184932847985635 1101 12-13 0.3465699329238632 1101 14-15 0.44605014075755633 1101 16-17 0.4006459496975552 1101 18-19 0.40672606449265203 1101 20-21 0.3167779346543327 1101 22-23 0.39280743641216986 1101 24-25 0.4323396743619128 1101 26-27 0.40644496531491114 1101 28-29 0.48306950272863247 1101 30-31 0.6510101346263397 1101 32-33 0.7404102356193718 1101 34-35 0.8139444451499926 1101 36-37 0.7746350355573455 1101 38-39 0.74600254160449 1101 40-41 1.0335906126855292 1101 42-43 1.0847524090161613 1101 44-45 1.2398166075826538 1101 46-47 1.2385391717129082 1101 48-49 1.396053327169973 1101 50-51 1.1538366698128542 1101 52-53 0.9942526719860396 1101 54-55 1.0424848993193407 1101 56-57 1.062128932861203 1101 58-59 1.0021129975170204 1101 60-61 1.252000214814263 1101 62-63 1.401290269940585 1101 64-65 1.2693898470731426 1101 66-67 0.9655393903271268 1101 68-69 0.8358133691304843 1101 70-71 0.9271032682635578 1101 72-73 1.4327236471142264 1101 74-75 1.3809110200498331 1101 76-77 1.1529690579628813 1101 78-79 1.456322805480621 1101 80-81 1.6670083260770383 1101 82-83 1.6262669092903153 1101 84-85 1.4690622798588429 1101 86-87 1.0872175399810438 1101 88-89 1.0225114272562017 1101 90-91 1.401541237926029 1101 92-93 1.2376987693090555 1101 94-95 1.2271691567829457 1101 96-97 1.08144687847944 1101 98-99 0.8791065682624328 1101 100-101 0.9066199551302638 1102 1 0.19308491169097763 1102 2 0.32058101049475596 1102 3 0.2978919986665751 1102 4 0.2700489478796797 1102 5 0.12389176740069274 1102 6 0.04937656146991998 1102 7 -0.004515439240293517 1102 8 0.11824030264794061 1102 9 0.1857352961106642 1102 10-11 0.26470878538251696 1102 12-13 0.24364739331309693 1102 14-15 0.36933236038830586 1102 16-17 0.4330403556997098 1102 18-19 0.39379232439163303 1102 20-21 0.4804252039956367 1102 22-23 0.38667133781216023 1102 24-25 0.5566528448295145 1102 26-27 0.3544642039790631 1102 28-29 0.4241642948725257 1102 30-31 0.6274812959669447 1102 32-33 0.5821683556029527 1102 34-35 0.5301317566158446 1102 36-37 0.6036602210422259 1102 38-39 0.5278246516400102 1102 40-41 0.5515366837379929 1102 42-43 0.8118754448398846 1102 44-45 0.6960554889020827 1102 46-47 0.5578044612002131 1102 48-49 0.8595609994314302 1102 50-51 0.8335019045620413 1102 52-53 0.6428685264064384 1102 54-55 0.8355640226764578 1102 56-57 0.794981621871333 1102 58-59 0.7346302667988205 1102 60-61 0.882021842321361 1102 62-63 0.9699304590250861 1102 64-65 1.0491161415999244 1102 66-67 0.8417211684830477 1102 68-69 0.9788022043389937 1102 70-71 0.9624182801928995 1102 72-73 1.0654758589507516 1102 74-75 1.1570767337575667 1102 76-77 0.9884508872870441 1102 78-79 1.2450559749962515 1102 80-81 1.2179527073875391 1102 82-83 1.0582808258622762 1102 84-85 1.2575593168606218 1102 86-87 1.2770911365730022 1102 88-89 1.3412613156975333 1102 90-91 1.5854334306042084 1102 92-93 1.643329804632991 1102 94-95 1.4563738773507957 1102 96-97 1.8002309038731639 1102 98-99 1.8166247895115326 1102 100-101 1.566550089655145 1103 1 0.19990589174514994 1103 2 0.2612298654565919 1103 3 0.2444733004805819 1103 4 0.24758937306231132 1103 5 0.12883718314394343 1103 6 0.0684215421807508 1103 7 0.06571356839329923 1103 8 0.08976619938930241 1103 9 0.2459975467878408 1103 10-11 0.24851306183330024 1103 12-13 0.32597933125941125 1103 14-15 0.3568816093417695 1103 16-17 0.29692650853562697 1103 18-19 0.4383698094117392 1103 20-21 0.46491486171540686 1103 22-23 0.4945255198691214 1103 24-25 0.30639326180398285 1103 26-27 0.4043975126879218 1103 28-29 0.40661730316276845 1103 30-31 0.48030387661210483 1103 32-33 0.3265180642133174 1103 34-35 0.5019572511574211 1103 36-37 0.34997886177111326 1103 38-39 0.2669355028260867 1103 40-41 0.4627900696035354 1103 42-43 0.6557398460546864 1103 44-45 0.4559288780508908 1103 46-47 0.4485312936716923 1103 48-49 0.6802188833680631 1103 50-51 0.7169850367954851 1103 52-53 0.8272453219411986 1103 54-55 0.7958519238502291 1103 56-57 0.8396052777853136 1103 58-59 0.6322519527546611 1103 60-61 0.7378763116638858 1103 62-63 1.0821780581386058 1103 64-65 0.7828424405410743 1103 66-67 0.6061511118467493 1103 68-69 0.7266157560093554 1103 70-71 0.48946005972175044 1103 72-73 0.7847455380117374 1103 74-75 1.1626788022136125 1103 76-77 0.9390408487086965 1103 78-79 1.1778927147155294 1103 80-81 1.0957434814169247 1103 82-83 0.9099409260018163 1103 84-85 0.9883450428722789 1103 86-87 0.7385517784510292 1103 88-89 0.5155615701504566 1103 90-91 0.7023022231840379 1103 92-93 0.4037398021705414 1103 94-95 0.24324287513459097 1103 96-97 0.2893253387414241 1103 98-99 -0.045785946760322105 1103 100-101 0.2704643554353332 1104 1 0.1885445066725815 1104 2 0.16390009242588377 1104 3 0.0717584261678752 1104 4 0.0661574997401786 1104 5 0.018374487363189473 1104 6 -0.03439798780188141 1104 7 0.025259629808516593 1104 8 0.20043131560159821 1104 9 -0.02138692282755983 1104 10-11 0.22247117085321833 1104 12-13 0.2068247595724344 1104 14-15 0.09814953647041591 1104 16-17 0.3036681138301489 1104 18-19 0.2908885799588461 1104 20-21 0.3071324421452317 1104 22-23 0.1576146566392964 1104 24-25 0.21885575659990764 1104 26-27 0.20975662950317542 1104 28-29 0.20247558014856537 1104 30-31 0.30947395284277945 1104 32-33 0.30569603627641584 1104 34-35 0.11478151863950359 1104 36-37 0.1383655278635345 1104 38-39 0.4248291818533261 1104 40-41 0.25519499660298095 1104 42-43 0.36860502830485586 1104 44-45 0.32956110453208254 1104 46-47 0.280405333610247 1104 48-49 0.4059600149126297 1104 50-51 0.2669742697512518 1104 52-53 0.37149715592773447 1104 54-55 0.17652436165260355 1104 56-57 0.32335278342006646 1104 58-59 0.19490681874084714 1104 60-61 0.21499634267297196 1104 62-63 0.24007245496878582 1104 64-65 0.3932843200363152 1104 66-67 0.39157899418449205 1104 68-69 0.3293771439531348 1104 70-71 0.18481659244638848 1104 72-73 0.45083509110762066 1104 74-75 0.790817395606954 1104 76-77 0.5512923094376951 1104 78-79 0.4698518860838057 1104 80-81 0.6210639804335365 1104 82-83 0.8351372451082959 1104 84-85 1.0556979291579331 1104 86-87 0.6141830861444397 1104 88-89 0.5107774134971415 1104 90-91 0.7299448147397989 1104 92-93 0.7106931593349373 1104 94-95 0.820188480027138 1104 96-97 0.86058809768884 1104 98-99 0.43139868651415014 1104 100-101 1.0851900829286194 1105 1 0.22360614670579793 1105 2 0.15607522628133097 1105 3 0.1759775683001088 1105 4 0.2003662270696296 1105 5 0.1942401318192566 1105 6 0.25107313291348277 1105 7 0.08564483987095883 1105 8 0.0665758657234079 1105 9 0.3821132767512623 1105 10-11 0.22922473459685477 1105 12-13 0.20267260251537067 1105 14-15 0.14263152620242892 1105 16-17 0.06028977203191488 1105 18-19 0.18121428341683554 1105 20-21 0.05629054068669603 1105 22-23 0.3013263180068009 1105 24-25 0.04702068670144399 1105 26-27 0.20901451745458388 1105 28-29 0.11075764237128283 1105 30-31 0.3738300932178049 1105 32-33 0.26239546139765935 1105 34-35 0.6114517639608721 1105 36-37 0.6092017860588825 1105 38-39 0.46507872419844176 1105 40-41 0.4878316392743116 1105 42-43 0.5593702639684466 1105 44-45 0.5399882949669816 1105 46-47 0.4705133355739193 1105 48-49 0.589608086338913 1105 50-51 0.4184649170748287 1105 52-53 0.9423212558731322 1105 54-55 1.0650848248589782 1105 56-57 1.1755658143112022 1105 58-59 1.0466793998226116 1105 60-61 0.8233435547633228 1105 62-63 1.0932466758084871 1105 64-65 0.7895102157322924 1105 66-67 0.9276121472086842 1105 68-69 1.1626428040084669 1105 70-71 0.8381298302571665 1105 72-73 1.1875722756239746 1105 74-75 1.3361906080001127 1105 76-77 1.1003921401397996 1105 78-79 1.4338233210784601 1105 80-81 1.277070385307848 1105 82-83 1.7821502154375786 1105 84-85 1.867188851762645 1105 86-87 1.5595306056320517 1105 88-89 1.4210598519374003 1105 90-91 1.1010662183065243 1105 92-93 1.2904042517881038 1105 94-95 1.2469453471443437 1105 96-97 1.2458268352495736 1105 98-99 0.9074343170685211 1105 100-101 0.9591962033457619 1106 1 -0.010779592125818027 1106 2 -0.04710346787674169 1106 3 -0.11446487156243279 1106 4 0.07326141607306624 1106 5 -0.012224644813045416 1106 6 0.0935559507828998 1106 7 0.08238023505996495 1106 8 0.02115060799145141 1106 9 0.15513733173407473 1106 10-11 0.16097945968276406 1106 12-13 0.11704115921640579 1106 14-15 0.2769420932127318 1106 16-17 -0.009222685012751697 1106 18-19 -0.28515169596460765 1106 20-21 -0.06297492323082565 1106 22-23 -0.21347179195883825 1106 24-25 -0.10630835109924419 1106 26-27 0.056984878279315865 1106 28-29 0.27993719563588826 1106 30-31 0.2847729626336104 1106 32-33 0.2923782792670764 1106 34-35 0.11865751997461871 1106 36-37 0.2819681090829391 1106 38-39 0.25003362110565774 1106 40-41 0.3835898007863321 1106 42-43 0.5906524804632909 1106 44-45 0.17353640493261224 1106 46-47 -0.03923752353260568 1106 48-49 0.2849903887444114 1106 50-51 0.508080466903003 1106 52-53 0.6542950531239953 1106 54-55 0.43724977331259396 1106 56-57 0.6981066218713359 1106 58-59 0.5967008774858478 1106 60-61 0.8135819568251712 1106 62-63 1.0235087032998962 1106 64-65 0.9757859889281697 1106 66-67 1.305747886040301 1106 68-69 0.9315216699878484 1106 70-71 0.6051186618722895 1106 72-73 1.3064189251085097 1106 74-75 1.3526317592028612 1106 76-77 1.6080596659129966 1106 78-79 1.7498349190166067 1106 80-81 1.8450042341050974 1106 82-83 1.562057861485691 1106 84-85 1.7430157417970094 1106 86-87 1.607705004257479 1106 88-89 1.6249365701504601 1106 90-91 1.3139957715711326 1106 92-93 1.426186038729682 1106 94-95 1.1132361547044844 1106 96-97 0.8928199623973399 1106 98-99 0.7093887844224724 1106 100-101 0.2555450005966229 1107 1 -0.031212815083875967 1107 2 0.039817061924580344 1107 3 -0.08260780754477182 1107 4 0.09470181342405937 1107 5 0.05922954944743708 1107 6 -0.04052241566301973 1107 7 -0.06916501880316872 1107 8 -0.24368106971274273 1107 9 0.026329384714209425 1107 10-11 0.02028547513530299 1107 12-13 0.03234193184334089 1107 14-15 -0.0601233041382514 1107 16-17 -0.032960267794216236 1107 18-19 0.05565956231354363 1107 20-21 -0.029876137358861854 1107 22-23 -0.09361803919944833 1107 24-25 -0.19740934447672487 1107 26-27 0.05209387386430819 1107 28-29 0.08221368570210785 1107 30-31 0.024418382059664623 1107 32-33 0.09938710928915384 1107 34-35 -0.13846858377813476 1107 36-37 -0.11389281806938101 1107 38-39 -0.026904844678007578 1107 40-41 -0.1761439188604612 1107 42-43 0.01919138774805873 1107 44-45 0.09332117314453825 1107 46-47 -0.19263156326770314 1107 48-49 0.0422889538658211 1107 50-51 0.07751341392287969 1107 52-53 -0.07636857822258492 1107 54-55 0.24928067838985157 1107 56-57 0.15314939229076074 1107 58-59 0.10488244481475917 1107 60-61 0.04334878905475392 1107 62-63 -0.077470877274056 1107 64-65 -0.06669469539854234 1107 66-67 0.08474898979305934 1107 68-69 0.14596703422625978 1107 70-71 0.07541391573542455 1107 72-73 0.10345948802241622 1107 74-75 0.23112927575915165 1107 76-77 0.4765613215421354 1107 78-79 0.4202198527914405 1107 80-81 0.824736574060946 1107 82-83 0.6560493074018012 1107 84-85 0.32434162479921724 1107 86-87 0.2601746510565981 1107 88-89 0.34392801606657386 1107 90-91 0.5184383764276426 1107 92-93 0.8521518223941413 1107 94-95 0.7675752827398021 1107 96-97 0.764619079395132 1107 98-99 0.27731096985293746 1107 100-101 0.12759246196527485 1108 1 0.18708433192481522 1108 2 -0.05142836239151194 1108 3 0.01583576134895992 1108 4 0.15517069877348888 1108 5 0.03722155771859548 1108 6 0.19972683685885073 1108 7 0.24925787219076767 1108 8 -0.07639686036297633 1108 9 -0.12060106489039413 1108 10-11 0.04364032466166634 1108 12-13 0.05915613811935572 1108 14-15 0.05704494131400395 1108 16-17 0.09846455127416931 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-2.3157392428576706 2316 90-91 -2.5031790251768378 2316 92-93 -2.047086318993891 2316 94-95 -1.8539640485475495 2316 96-97 -1.6435621514238008 2316 98-99 -1.5318205651710208 2316 100-101 -1.7614265441188266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 10.0 5 17.0 6 58.0 7 126.0 8 312.0 9 643.0 10 1038.0 11 1701.0 12 2523.0 13 3413.0 14 4513.0 15 6474.0 16 8826.0 17 11886.0 18 10497.0 19 5869.0 20 4735.0 21 4770.0 22 4869.0 23 5241.0 24 5745.0 25 6857.0 26 8019.0 27 9300.0 28 11447.0 29 13270.0 30 16239.0 31 19441.0 32 24295.0 33 30811.0 34 40346.0 35 53913.0 36 73162.0 37 89400.0 38 46366.0 39 1802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91858991240951 20.841156732607423 16.538176609567692 18.70207674541538 2 15.654982336171544 22.865802259833494 40.360095848763535 21.119119555231432 3 18.665070519644352 29.10796343510033 32.04164125332899 20.185324791926323 4 12.15500275600022 19.395043745584243 38.3138961129979 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26.464501230856367 35.71557793418825 18.366233522673994 26-27 19.513932299066127 26.5992309295145 35.62387528177152 18.262961489647857 28-29 18.9043978649008 26.932423883107486 35.60616966386204 18.557008588129666 30-31 19.17721812707592 26.65569295842145 35.57565086938857 18.591438045114064 32-33 19.121028784446246 26.90245186807192 35.23461710397709 18.741902243504747 34-35 19.312174323169913 26.885208328992555 35.32803979247603 18.47457755536151 36-37 19.635286629512745 26.657245583393635 35.09454549587607 18.612922291217554 38-39 19.343750503193444 27.08170535454655 34.9667584491507 18.607785693109307 40-41 19.332591452586716 27.212690896176156 34.73905528590032 18.715662365336804 42-43 19.395706700963558 27.53395752160761 34.04967240533471 19.020663372094123 44-45 19.579818880590373 27.675545849046955 33.617804329666875 19.1268309406958 46-47 20.01750205516557 28.04742223518671 32.838231004398246 19.09684470524948 48-49 19.978216499580903 28.538374477314377 31.868503426165773 19.614905596938943 50-51 19.940235459031456 28.988549076064825 31.39419024256258 19.67702522234114 52-53 20.109709644485445 29.277340753382493 30.979052504171882 19.633897097960183 54-55 19.975148032110564 29.113739425601317 30.99298007675318 19.918132465534942 56-57 20.004678703752813 29.41133572256613 30.665872360415513 19.918113213265546 58-59 20.057563577541302 28.918831377926953 30.931094181513743 20.092510863018006 60-61 19.817778872198964 28.898338794915997 31.464398689220165 19.819483643664878 62-63 19.853479506547014 28.731560627944972 31.504273909028484 19.91068595647953 64-65 19.766582881734443 28.969161209912507 31.301059402696147 19.963196505656903 66-67 19.79012070900558 28.962016205030096 30.962215097860007 20.285647988104312 68-69 19.829449347175895 28.929872314310227 30.96685778795576 20.273820550558117 70-71 19.858845858427244 29.07326396222252 30.74506542964218 20.32282474970806 72-73 20.004773278731065 29.019640526651752 30.47890002443464 20.49668617018254 74-75 19.77927374185275 29.464104754526566 30.07260308482609 20.684018418794594 76-77 19.65066039826571 29.314845086269436 30.25936708597016 20.775127429494695 78-79 19.725000899742202 29.205347688799062 30.03907722439949 21.030574187059244 80-81 19.560148353563363 29.57922292433817 29.883336364049644 20.977292358048825 82-83 19.537111674602805 29.297758210281334 29.93343872754019 21.231691387575673 84-85 19.45892546609736 29.314313100736133 29.67849606170936 21.54826537145715 86-87 19.302098931354973 29.197696962464352 29.632144781843166 21.86805932433751 88-89 19.2333773409428 29.086693727873865 29.666211437306206 22.013717493877135 90-91 19.066971122474012 29.330516075323455 29.643621912862628 21.958890889339912 92-93 19.12828779136944 29.612852266743445 29.279574495490003 21.979285446397114 94-95 18.85880755163002 29.684679480015685 28.97276011054307 22.483752857811222 96-97 19.12152876762668 29.75961706935693 28.673958689481626 22.444895473534768 98-99 19.146987568404082 30.134355187913524 28.101790202849248 22.616867040833142 100-101 19.008292640423686 30.334527918050984 27.825142346260357 22.832037095264972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1139.0 1 818.0 2 533.0 3 618.0 4 594.0 5 579.0 6 876.5 7 1647.0 8 2652.0 9 3072.5 10 2716.5 11 2318.0 12 2258.5 13 2340.0 14 2383.5 15 2479.5 16 2563.0 17 2601.5 18 2669.0 19 2753.5 20 2919.5 21 3149.5 22 3524.0 23 4010.5 24 4522.0 25 5203.5 26 6036.0 27 6971.5 28 7729.5 29 8517.5 30 9470.0 31 10269.5 32 11178.5 33 12168.5 34 13153.0 35 13891.5 36 14857.5 37 15623.5 38 15808.0 39 15651.5 40 15522.0 41 15770.0 42 15878.5 43 16492.5 44 17363.5 45 17477.0 46 17063.5 47 16859.5 48 17761.5 49 17485.5 50 15470.5 51 14199.5 52 13219.5 53 12504.0 54 11843.5 55 11048.0 56 10035.5 57 8819.0 58 8058.5 59 7315.0 60 6250.5 61 5099.5 62 4163.0 63 3161.5 64 2158.0 65 1500.0 66 1065.0 67 792.5 68 559.5 69 357.5 70 241.5 71 169.5 72 129.5 73 114.5 74 85.0 75 75.0 76 60.0 77 26.5 78 15.0 79 10.0 80 4.5 81 6.0 82 5.5 83 2.5 84 2.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026897046817908887 2 0.0045459797438719245 3 1.8941582266133019E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010986117714357152 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.629553793146557E-4 20-21 3.7883164532266037E-4 22-23 0.0016100344926213064 24-25 0.010323162335042494 26-27 0.005493058857178576 28-29 1.8941582266133019E-4 30-31 0.0019888661379439667 32-33 0.002841237339919953 34-35 0.0 36-37 0.00852371201975986 38-39 0.011459657271010476 40-41 2.841237339919953E-4 42-43 0.0 44-45 0.0 46-47 1.8941582266133019E-4 48-49 0.0031253610739119483 50-51 0.0064401379704852255 52-53 0.0 54-55 0.0024624056945972924 56-57 0.002841237339919953 58-59 1.8941582266133019E-4 60-61 0.0017047424039519717 62-63 0.005019519300525251 64-65 0.0 66-67 0.0031253610739119483 68-69 3.7883164532266037E-4 70-71 0.001041787024637316 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018941582266133019 80-81 0.002083574049274632 82-83 0.01505855790157575 84-85 0.00852371201975986 86-87 0.00520893512318658 88-89 0.0 90-91 4.7353955665332547E-4 92-93 1.8941582266133019E-4 94-95 0.0 96-97 0.0019888661379439667 98-99 0.0018941582266133019 100-101 1.8941582266133019E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527939.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.688946311673554 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06965186429701 51.448599995386544 2 10.732695299086313 13.45642718767946 3 3.180035659385501 5.980592541612751 4 1.313218586977753 3.2929715797816064 5 0.7312451907389362 2.292049525145133 6 0.44814336603074545 1.6856181247820443 7 0.28603420385358586 1.255182799407581 8 0.20510119665398457 1.028606232440131 9 0.1507835472600108 0.8507215528979859 >10 0.8047972869687852 8.996957113221445 >50 0.05108746095693808 2.197110359572746 >100 0.023881118727165213 2.5323429157428565 >500 0.0012091705684640344 0.4627689441176361 >1k 0.001511463210580043 1.836673466989406 >5k 6.045852842320172E-4 2.6833776612226568 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8035 1.521956135083788 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6125 1.1601719138006474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4449 0.842710995020258 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.2727587846323155 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1403 0.26575039919384624 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1241 0.23506503592271077 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1159 0.21953293846448171 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 751 0.14225128281865898 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.1142177410647821 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.11024000878889417 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8941582266133019E-4 0.0 18-19 0.0 0.0 0.0 1.8941582266133019E-4 0.0 20-21 0.0 0.0 0.0 3.7883164532266037E-4 0.0 22-23 0.0 0.0 0.0 6.629553793146557E-4 0.0 24-25 0.0 0.0 0.0 0.0019888661379439667 0.0 26-27 0.0 0.0 0.0 0.003125361073911948 0.0 28-29 0.0 0.0 0.0 0.007860756640445204 0.0 30-31 0.0 0.0 0.0 0.017520963596173042 0.0 32-33 0.0 0.0 0.0 0.030211823714482167 0.0 34-35 0.0 0.0 0.0 0.049153405980615184 0.0 36-37 0.0 0.0 0.0 0.07472454203989476 0.0 38-39 0.0 0.0 0.0 0.11279712239482212 0.0 40-41 0.0 0.0 0.0 0.15854104356753337 0.0 42-43 0.0 0.0 0.0 0.23089788782416149 0.0 44-45 0.0 0.0 0.0 0.3193550770070027 0.0 46-47 0.0 0.0 0.0 0.42220786871210503 0.0 48-49 0.0 0.0 0.0 0.5430551635700336 0.0 50-51 0.0 0.0 0.0 0.6842646593640553 0.0 52-53 0.0 0.0 0.0 0.8487723013454206 0.0 54-55 0.0 0.0 0.0 0.9998314199178314 0.0 56-57 0.0 0.0 0.0 1.2115036017418679 0.0 58-59 0.0 0.0 0.0 1.4600171610735333 0.0 60-61 0.0 0.0 0.0 1.7305976637452432 0.0 62-63 0.0 0.0 0.0 2.0447438056290594 0.0 64-65 0.0 0.0 0.0 2.3453467161925907 0.0 66-67 0.0 0.0 0.0 2.69841780963331 0.0 68-69 0.0 0.0 0.0 3.113806708729607 0.0 70-71 0.0 0.0 0.0 3.5901875027228525 0.0 72-73 0.0 0.0 0.0 4.139209264706718 0.0 74-75 0.0 0.0 0.0 4.677339616887557 0.0 76-77 0.0 0.0 0.0 5.224372512733479 0.0 78-79 0.0 0.0 0.0 5.862419711368169 0.0 80-81 0.0 0.0 0.0 6.6320161988411535 0.0 82-83 0.0 0.0 0.0 7.342136117998481 0.0 84-85 0.0 0.0 0.0 8.15671886335353 0.0 86-87 0.0 0.0 0.0 9.058054055487471 0.0 88-89 0.0 0.0 0.0 10.000018941582265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3065 0.0 49.760292 1 GTATCAA 5875 0.0 45.207687 1 TATCAAC 7695 0.0 34.508755 2 ATCAACG 7705 0.0 34.399055 3 TCAACGC 7755 0.0 34.17403 4 CAACGCA 7915 0.0 33.42637 5 AACGCAG 8185 0.0 32.381763 6 CGCAGAG 9265 0.0 28.196953 8 ACGCAGA 9315 0.0 28.147587 7 GCAGAGT 9470 0.0 26.884365 9 CATGGGT 365 0.0 26.024412 4 GAGTACT 5780 0.0 24.815495 12-13 GTATAGT 135 1.3381954E-5 24.636139 1 GTACATG 4665 0.0 24.443758 1 TACATGG 4735 0.0 23.977507 2 AGTACTT 5850 0.0 23.869059 12-13 CTAAGGG 160 1.8551764E-6 23.751778 2 ACAGTAC 185 2.5367262E-7 23.107647 8 GTACTTT 6150 0.0 23.052237 14-15 ACATGGG 4890 0.0 22.341068 3 >>END_MODULE