FastQCFastQC Report
Thu 26 May 2016
SRR936780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107341.8838916209471055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72471.2718988799146336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.8992976211787749No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.2825661924468828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.2239468705355419No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.21517152294402384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.14619729087469155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.13987904060879852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.12127530371478014No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.10302258072442248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.050.6066251
GTATCAA65900.042.2610471
TATCAAC83850.032.996092
ATCAACG84300.032.8763433
TCAACGC86200.032.151694
CAACGCA87350.031.7828245
AACGCAG89100.031.1558426
GTACATG46100.028.0411621
ACGCAGA98450.028.0037787
CGCAGAG98500.027.7965328
TACATGG46400.027.252492
GCAGAGT100000.026.8021139
ATGGGAT14100.025.9605665
GAGTACT62400.025.36224612-13
ACATGGG48200.024.9526123
CATGGGA37400.024.150414
AGTACTT64100.024.02232612-13
GTGTAAG1801.9373147E-723.7628231
GTACTTT67550.023.53003514-15
AGAGTAC90600.022.58056810-11
Produced by FastQC (version 0.11.5)