##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936780_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569778 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0226000301872 33.0 31.0 34.0 31.0 34.0 2 32.069281369235036 33.0 31.0 34.0 30.0 34.0 3 32.05214487045832 33.0 31.0 34.0 30.0 34.0 4 35.708772539480286 37.0 35.0 37.0 35.0 37.0 5 35.72614772771149 37.0 35.0 37.0 35.0 37.0 6 35.824658024704355 37.0 35.0 37.0 35.0 37.0 7 35.79648213865751 37.0 35.0 37.0 35.0 37.0 8 35.766642446707316 37.0 35.0 37.0 35.0 37.0 9 37.41291169543226 39.0 37.0 39.0 35.0 39.0 10-11 37.31321233884074 39.0 37.0 39.0 34.5 39.0 12-13 37.23317765866706 39.0 37.0 39.0 34.0 39.0 14-15 38.308971213349764 40.0 38.0 41.0 33.5 41.0 16-17 38.299204251480404 40.0 38.0 41.0 34.0 41.0 18-19 38.397799669344906 40.0 38.0 41.0 34.0 41.0 20-21 38.41617436966678 40.0 38.0 41.0 34.0 41.0 22-23 38.3038788791424 40.0 38.0 41.0 34.0 41.0 24-25 38.25840239531888 40.0 38.0 41.0 34.0 41.0 26-27 38.21973821383065 40.0 38.0 41.0 34.0 41.0 28-29 38.10442137113051 40.0 38.0 41.0 34.0 41.0 30-31 37.80575680352699 40.0 37.0 41.0 33.5 41.0 32-33 37.43218323627799 40.0 36.0 41.0 33.0 41.0 34-35 37.20525187002657 39.5 35.0 41.0 33.0 41.0 36-37 36.981209348202285 39.0 35.0 41.0 32.5 41.0 38-39 36.77243768625675 39.0 35.0 41.0 31.5 41.0 40-41 36.51378431599676 39.0 35.0 41.0 31.0 41.0 42-43 36.14482394897662 39.0 35.0 41.0 30.5 41.0 44-45 35.659112847459895 39.0 35.0 41.0 29.0 41.0 46-47 34.97294999104915 39.0 35.0 40.0 25.0 41.0 48-49 34.400816633846865 38.5 35.0 40.0 20.5 41.0 50-51 33.58267167212493 38.0 34.0 40.0 15.5 40.5 52-53 33.71295750274668 38.0 34.0 40.0 13.5 40.5 54-55 34.04762644398345 39.0 35.0 40.5 10.0 41.0 56-57 33.91242115350189 39.0 34.5 41.0 7.5 41.0 58-59 33.69432568474038 38.0 34.0 40.5 6.0 41.0 60-61 33.41420430413248 38.0 34.0 40.0 2.0 41.0 62-63 33.12752773887374 38.0 34.0 40.0 2.0 41.0 64-65 32.79961844788672 37.0 33.5 40.0 2.0 41.0 66-67 32.418337492848096 36.5 33.0 40.0 2.0 41.0 68-69 32.022726395192514 36.0 33.0 39.0 2.0 41.0 70-71 31.590691462288824 35.5 32.0 39.0 2.0 41.0 72-73 31.20266928523039 35.0 32.0 38.5 2.0 40.0 74-75 30.806550094949262 35.0 31.0 37.5 2.0 40.0 76-77 30.35939787074966 35.0 31.0 37.0 2.0 39.0 78-79 29.910416864111987 35.0 31.0 36.5 2.0 39.0 80-81 29.477737820695076 35.0 30.0 36.0 2.0 38.5 82-83 29.075821635794995 35.0 29.5 36.0 2.0 37.0 84-85 28.704653391320832 34.0 29.0 35.0 2.0 37.0 86-87 28.337128671166667 34.0 29.0 35.0 2.0 36.5 88-89 27.969317874681018 34.0 29.0 35.0 2.0 36.0 90-91 27.59214290478046 34.0 27.5 35.0 2.0 36.0 92-93 27.207087672742716 34.0 26.5 35.0 2.0 35.0 94-95 26.850049492960416 34.0 25.5 35.0 2.0 35.0 96-97 26.409405944069444 34.0 25.0 35.0 2.0 35.0 98-99 25.910701185373952 34.0 23.5 35.0 2.0 35.0 100-101 24.00575487295052 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29328129656473934 1101 2 0.2931420680051602 1101 3 0.25020471768031527 1101 4 0.1751461280071993 1101 5 0.15524636990490137 1101 6 0.12854108228440708 1101 7 0.11506680183229889 1101 8 0.03551024729004837 1101 9 0.09017129233212984 1101 10-11 0.16254558584947176 1101 12-13 0.31892879225149073 1101 14-15 0.4898720656686564 1101 16-17 0.47677772881274194 1101 18-19 0.36950307849669883 1101 20-21 0.31014660732451915 1101 22-23 0.4756918881990586 1101 24-25 0.42632262762014506 1101 26-27 0.3592614629571287 1101 28-29 0.47311832834476775 1101 30-31 0.28133512079306655 1101 32-33 0.2922243126425528 1101 34-35 0.451445809491716 1101 36-37 0.2417361982607602 1101 38-39 0.28666568341978405 1101 40-41 0.4724095849342689 1101 42-43 0.4980784039168853 1101 44-45 0.6117983555991984 1101 46-47 0.7308568268707845 1101 48-49 1.0310618387128798 1101 50-51 1.1513903603940179 1101 52-53 0.971468974743658 1101 54-55 1.1469847508504003 1101 56-57 1.2979049365044517 1101 58-59 1.134208027181522 1101 60-61 1.1878401555071463 1101 62-63 1.1836407275050007 1101 64-65 0.9804211648626264 1101 66-67 1.0577994638544688 1101 68-69 0.9724763299005517 1101 70-71 1.067859322238494 1101 72-73 1.0451051791813768 1101 74-75 1.0317398587958095 1101 76-77 1.0752335866566334 1101 78-79 1.0497977656507445 1101 80-81 1.1882554109919603 1101 82-83 1.1906682480269062 1101 84-85 1.290994745271533 1101 86-87 1.3993464142459047 1101 88-89 1.1885922118851049 1101 90-91 1.1880103682146412 1101 92-93 1.2211934437487635 1101 94-95 1.157719313762648 1101 96-97 1.296300000017844 1101 98-99 1.487313722858584 1101 100-101 1.638245547094634 1102 1 0.16702621258199812 1102 2 0.20681371257655456 1102 3 0.34680194205859394 1102 4 0.31123464363948017 1102 5 0.2280187316671416 1102 6 0.205417886904236 1102 7 0.22097586451664597 1102 8 0.21010197630988614 1102 9 0.500383500100142 1102 10-11 0.3146494232862125 1102 12-13 0.16381841937434416 1102 14-15 0.19849310326684844 1102 16-17 0.3555627733654987 1102 18-19 0.37564654771338013 1102 20-21 0.32605986138394627 1102 22-23 0.19849180437154956 1102 24-25 0.3962126428332837 1102 26-27 0.372860534645163 1102 28-29 0.23850494388700838 1102 30-31 0.35446530179837055 1102 32-33 0.33528564643124525 1102 34-35 0.3645659777676613 1102 36-37 0.33691572882676013 1102 38-39 0.32244450300436966 1102 40-41 0.19816442053922856 1102 42-43 0.4347991186239568 1102 44-45 0.7414212870779764 1102 46-47 0.5470883149641708 1102 48-49 0.7871913366792853 1102 50-51 0.7766794876565868 1102 52-53 0.8939421188959145 1102 54-55 0.6892210046924987 1102 56-57 0.8279015989279941 1102 58-59 0.5968240661768291 1102 60-61 0.7193546390365952 1102 62-63 0.6855641029590629 1102 64-65 0.8968831785855045 1102 66-67 0.8265081322390735 1102 68-69 0.8956724862543695 1102 70-71 0.8532783821285896 1102 72-73 0.8973304888774258 1102 74-75 1.096702291656193 1102 76-77 0.7897718368351541 1102 78-79 0.968039949512395 1102 80-81 1.072444612767864 1102 82-83 1.2152933528112833 1102 84-85 1.0553339051336827 1102 86-87 1.1894326479073065 1102 88-89 1.0008713885127563 1102 90-91 0.8161267332057918 1102 92-93 1.075540831124961 1102 94-95 1.3164730782384169 1102 96-97 1.0673756310526485 1102 98-99 1.081853292544075 1102 100-101 1.0605863524595947 1103 1 0.18447760408934855 1103 2 0.23233374290381903 1103 3 0.252415434034166 1103 4 0.16399291071660826 1103 5 0.18437145080553563 1103 6 0.16695185313974292 1103 7 0.26276326264233063 1103 8 0.3300745760702313 1103 9 0.20461844292653808 1103 10-11 0.24436465796174645 1103 12-13 0.34587807181002717 1103 14-15 0.47563951465868115 1103 16-17 0.31341762730372835 1103 18-19 0.3018147929089352 1103 20-21 0.2442560190491534 1103 22-23 0.39368802200019104 1103 24-25 0.2328402384114412 1103 26-27 0.4345131065174357 1103 28-29 0.33514058879372755 1103 30-31 0.2596316088538302 1103 32-33 0.4591695897485195 1103 34-35 0.5283418649469738 1103 36-37 0.3196660786981411 1103 38-39 0.24233894703663594 1103 40-41 0.4658655749877454 1103 42-43 0.8010176517964354 1103 44-45 0.79953296744646 1103 46-47 0.6339123161918963 1103 48-49 0.5211417174470796 1103 50-51 0.5617929049319486 1103 52-53 0.5479988483683442 1103 54-55 0.7560400868861024 1103 56-57 0.5929150703737207 1103 58-59 0.8686052748566837 1103 60-61 0.7213466445898149 1103 62-63 0.9386514574842266 1103 64-65 0.9169175711711759 1103 66-67 0.8077360207233575 1103 68-69 0.7146647347103112 1103 70-71 0.6608120251713565 1103 72-73 0.7010041129961415 1103 74-75 0.7806711188019406 1103 76-77 0.7041572675504604 1103 78-79 1.0640912050348774 1103 80-81 1.0404388766049344 1103 82-83 1.0246243743340884 1103 84-85 1.0000181468452602 1103 86-87 0.9646302477438766 1103 88-89 0.9777941058115971 1103 90-91 1.1223701461211064 1103 92-93 1.0625353724199513 1103 94-95 1.1116110601945053 1103 96-97 1.0652469291999793 1103 98-99 1.1229312927737674 1103 100-101 1.0022226224088726 1104 1 0.0815084986062864 1104 2 -0.006513021910706129 1104 3 0.033501499848661354 1104 4 0.04844749568533757 1104 5 0.17816095980082736 1104 6 -0.15931429472424696 1104 7 -0.012730359139027314 1104 8 0.09209842863698725 1104 9 0.08416128395858635 1104 10-11 0.09135006369946552 1104 12-13 -0.09418377204533357 1104 14-15 -0.12673047031761797 1104 16-17 0.23408115000177787 1104 18-19 0.08819700568677291 1104 20-21 -0.036635561023494745 1104 22-23 -0.20555387518864165 1104 24-25 -0.3250904071898617 1104 26-27 -0.1964071243071217 1104 28-29 -0.11775600875001402 1104 30-31 0.10475112621592331 1104 32-33 0.0349707339512193 1104 34-35 0.015356352176830512 1104 36-37 -0.07728519413925738 1104 38-39 0.0858562412570123 1104 40-41 0.3771291710421707 1104 42-43 0.38198376298223735 1104 44-45 0.47205095737758285 1104 46-47 0.38831924562750686 1104 48-49 0.47647134467403873 1104 50-51 0.33941869086971366 1104 52-53 0.604670557741386 1104 54-55 0.6706105931886199 1104 56-57 0.7570355836594445 1104 58-59 0.7299415093777739 1104 60-61 0.7690654834707757 1104 62-63 0.7129859964551173 1104 64-65 0.7728799949595242 1104 66-67 0.607480970294116 1104 68-69 0.6561349686022524 1104 70-71 0.6756960581530045 1104 72-73 0.6182171453682237 1104 74-75 0.6610362820981734 1104 76-77 0.5596204242498395 1104 78-79 0.6731096002533974 1104 80-81 0.5619086667039106 1104 82-83 0.6316607118412527 1104 84-85 0.518300459215471 1104 86-87 0.8532525707912235 1104 88-89 0.8730772391987927 1104 90-91 0.7475621844752567 1104 92-93 0.9333400604400168 1104 94-95 0.994148198486922 1104 96-97 0.8420484349634165 1104 98-99 0.7984814328948673 1104 100-101 0.7309496022802477 1105 1 0.05047357801878505 1105 2 0.03167145110622016 1105 3 0.11851190021613078 1105 4 0.08392451218028185 1105 5 0.07024734446878256 1105 6 0.06320875600042086 1105 7 -0.17803084946874037 1105 8 0.02813523510799598 1105 9 0.004870152092387059 1105 10-11 -0.04237961637253562 1105 12-13 -0.06734447546120492 1105 14-15 0.21760571735952539 1105 16-17 0.22922266595336538 1105 18-19 0.11637229202364807 1105 20-21 0.11459549144887404 1105 22-23 -0.002237894473203994 1105 24-25 -0.11597953591167709 1105 26-27 0.16409164520057828 1105 28-29 0.10734379369902314 1105 30-31 0.188147322332874 1105 32-33 0.32159888627085564 1105 34-35 -0.048779558406153 1105 36-37 0.08624331257710338 1105 38-39 0.1639047439402006 1105 40-41 0.28578425601895674 1105 42-43 0.637371601460984 1105 44-45 0.5080535938800708 1105 46-47 0.4414600819141512 1105 48-49 0.6464818757463036 1105 50-51 0.6862980204661326 1105 52-53 0.6876942451851349 1105 54-55 0.6513447060204598 1105 56-57 0.5705842562588543 1105 58-59 0.661968479379162 1105 60-61 0.854997352661421 1105 62-63 0.8107774588177392 1105 64-65 0.8387122670943796 1105 66-67 1.0786344214609365 1105 68-69 0.939270502008533 1105 70-71 0.8010298221897649 1105 72-73 0.7367458574778389 1105 74-75 1.0353822913072577 1105 76-77 0.8413443458419003 1105 78-79 1.0498428392303154 1105 80-81 0.6473139822813074 1105 82-83 0.695623905370244 1105 84-85 0.7258354040767152 1105 86-87 0.34986171489886075 1105 88-89 0.4790414858350118 1105 90-91 0.7484089648426533 1105 92-93 0.6516656199499131 1105 94-95 0.7281023173685384 1105 96-97 0.5484854595247128 1105 98-99 0.8194670217573261 1105 100-101 1.093179276750618 1106 1 0.3103163820293311 1106 2 0.19624578218071775 1106 3 0.3601134697687378 1106 4 0.2367031258223946 1106 5 0.27551690068500534 1106 6 0.19975622172663776 1106 7 0.17148949814382775 1106 8 0.21252155689035135 1106 9 0.40526254024370445 1106 10-11 0.30767635310304087 1106 12-13 0.4571436746573525 1106 14-15 0.17998580426267807 1106 16-17 0.4053971222069066 1106 18-19 0.5674050191212956 1106 20-21 0.4749004949996234 1106 22-23 0.3842463799096336 1106 24-25 0.3943778261807438 1106 26-27 0.243481036276755 1106 28-29 0.5719536322902812 1106 30-31 0.7882815287097813 1106 32-33 0.7113751287330317 1106 34-35 0.769488156233777 1106 36-37 0.7442728259435754 1106 38-39 0.8762878399549336 1106 40-41 0.9544539879672982 1106 42-43 1.2138331269601963 1106 44-45 1.3703416019685548 1106 46-47 1.5690524315488403 1106 48-49 1.2849912822291358 1106 50-51 1.3595175291383939 1106 52-53 1.4431226824933034 1106 54-55 1.5098863701795366 1106 56-57 1.4895849904820864 1106 58-59 1.6915721192005364 1106 60-61 1.5558134237245937 1106 62-63 1.4917695027266973 1106 64-65 1.4214538084817079 1106 66-67 1.3805777057256883 1106 68-69 1.6741888347199847 1106 70-71 1.474691670265738 1106 72-73 1.467406297289589 1106 74-75 1.5301494462524339 1106 76-77 1.5542931548355305 1106 78-79 1.6849940771788212 1106 80-81 1.9525402072906388 1106 82-83 1.85984316127713 1106 84-85 1.8923506121266946 1106 86-87 2.038530002473639 1106 88-89 1.8334065031073123 1106 90-91 1.7006694094689792 1106 92-93 2.1157217820086274 1106 94-95 1.9338984681129894 1106 96-97 2.171242166834446 1106 98-99 2.088647099031313 1106 100-101 2.442618378350261 1107 1 -0.09300104134647214 1107 2 0.020641269538213436 1107 3 -0.019772925245266748 1107 4 0.04307171088807138 1107 5 -0.06957796736753608 1107 6 -0.04101337316113529 1107 7 -0.040306880697244196 1107 8 0.10172711091188802 1107 9 0.18861428179236128 1107 10-11 0.16043364078399236 1107 12-13 0.17383137928438686 1107 14-15 0.0769129471761616 1107 16-17 0.06375872529948623 1107 18-19 0.11830537808149444 1107 20-21 0.2971954735014677 1107 22-23 0.49859591247244595 1107 24-25 0.1925514682461369 1107 26-27 0.214295797035696 1107 28-29 0.17410443501994877 1107 30-31 0.28446511121145335 1107 32-33 -0.004913183365111706 1107 34-35 0.07914063542592231 1107 36-37 0.13457794211256413 1107 38-39 0.15906268118407496 1107 40-41 0.17870549678352177 1107 42-43 0.22142158500918185 1107 44-45 -0.048581714665893116 1107 46-47 -0.017586162589459775 1107 48-49 0.23573287033217127 1107 50-51 -0.36228338601288357 1107 52-53 -0.407184011522709 1107 54-55 -0.32283719102120045 1107 56-57 -0.2587582701387987 1107 58-59 -0.41797977224359073 1107 60-61 -0.2894195059428668 1107 62-63 -0.20795141558026842 1107 64-65 0.0805648889124555 1107 66-67 -0.24764208836527146 1107 68-69 -0.2674949252894834 1107 70-71 -0.38022036476246157 1107 72-73 -0.37946364866914806 1107 74-75 -0.314195944461936 1107 76-77 -0.4812379879201778 1107 78-79 -0.1002461500311469 1107 80-81 -0.43861300165575656 1107 82-83 -0.4955422278593957 1107 84-85 -0.4783201700909707 1107 86-87 -0.2721283541986814 1107 88-89 -0.3232490528865313 1107 90-91 -0.4809851603704729 1107 92-93 -0.7904801429647463 1107 94-95 -0.6601264224238719 1107 96-97 -0.37363771956056624 1107 98-99 -0.38074759940267455 1107 100-101 -0.3194915975875716 1108 1 0.12359631135254645 1108 2 0.30655083064358024 1108 3 0.27966341497723946 1108 4 0.2897361960999163 1108 5 0.03991347218153862 1108 6 0.2812596323910199 1108 7 0.18807560573903714 1108 8 0.13728306522800438 1108 9 0.05074457797763188 1108 10-11 0.06286455551726533 1108 12-13 0.25042683200663163 1108 14-15 0.2675545060015594 1108 16-17 0.2650480706519289 1108 18-19 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-1.3302185631095043 2316 64-65 -1.4485052692345803 2316 66-67 -1.5741283569016282 2316 68-69 -1.521499002167758 2316 70-71 -1.085010696736834 2316 72-73 -1.4084162045582218 2316 74-75 -1.385261308916931 2316 76-77 -1.2322124066290172 2316 78-79 -1.3242224503761122 2316 80-81 -1.1964475520791247 2316 82-83 -1.7082735123000568 2316 84-85 -1.2710545905918451 2316 86-87 -1.4113582274122471 2316 88-89 -1.3041022852685948 2316 90-91 -1.9798483385434977 2316 92-93 -1.8602070817219705 2316 94-95 -2.011460368035628 2316 96-97 -2.1776563567183977 2316 98-99 -2.452333773857223 2316 100-101 -2.6528323977309363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1140.0 3 370.0 4 333.0 5 310.0 6 394.0 7 481.0 8 583.0 9 693.0 10 944.0 11 1379.0 12 2321.0 13 3249.0 14 3838.0 15 5468.0 16 8055.0 17 12265.0 18 10580.0 19 4712.0 20 4220.0 21 4003.0 22 3981.0 23 4068.0 24 4436.0 25 4838.0 26 5406.0 27 6209.0 28 7350.0 29 8525.0 30 10351.0 31 13158.0 32 17091.0 33 22810.0 34 32869.0 35 49054.0 36 71643.0 37 108682.0 38 110528.0 39 23420.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40854398320068 19.457393331893595 15.989198433140725 19.144864251764997 2 14.942992882449813 21.38864211749752 42.47425286791274 21.194112132139935 3 18.000354627982958 28.967663896940195 32.07645217525645 20.955529299820405 4 11.254425276052935 18.026151274198536 39.612191284313454 31.107232165435082 5 11.372141810476602 38.77368866774194 34.0275215495279 15.826647972253555 6 26.524766360067343 39.068919826092454 18.642164495165524 15.764149318674676 7 24.43615096858041 37.15871371116458 21.680043555383833 16.725091764871177 8 25.87402144734438 33.24180264507878 20.408883922924485 20.47529198465236 9 27.902822373829277 16.09515411692792 21.349954681824247 34.65206882741855 10-11 23.37281588530093 28.33943166907028 27.103223188548363 21.184529257080424 12-13 26.222977512547917 26.806571025833563 28.310301369622398 18.66015009199612 14-15 22.93548897007166 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19.233435656580017 40-41 18.990133365862306 26.33688373027748 35.46327897309076 19.209703930769454 42-43 18.84861504547481 26.507373202332186 35.00094775750045 19.643063994692557 44-45 19.08168449983897 26.589080008389328 34.681008088330145 19.648227403441552 46-47 19.350547236438434 27.05476927613151 33.844590467766054 19.750093019664007 48-49 19.481008392742435 27.434983449694446 33.01777183394235 20.066236323620778 50-51 19.52802090664495 27.994931296959656 32.399332363353 20.0777154330424 52-53 19.57939779326008 28.250676387812646 31.909269694227955 20.260656124699324 54-55 19.351013963889248 28.16824770786325 32.05463390317516 20.42610442507234 56-57 19.45820330189176 28.348755735132535 31.655562069316556 20.53747889365915 58-59 19.599528568056186 27.868104364431286 31.91538094983181 20.616986117680717 60-61 19.54732293608253 27.85099496986482 32.33555529813994 20.266126795912708 62-63 19.509177041336617 27.64784453177519 32.54071615499783 20.302262271890363 64-65 19.450265547610773 27.84616842703847 32.39163095024975 20.311935075101005 66-67 19.42588561598146 27.95176069936453 32.0481515289822 20.574202155671802 68-69 19.51455057693979 27.803116168449037 32.16438240708956 20.51795084752161 70-71 19.46958368711689 27.91314968490946 31.941409052234697 20.675857575738952 72-73 19.56348516839458 27.88554188320407 31.70046141992372 20.85051152847763 74-75 19.402007875985266 28.291585228534345 31.39625403606945 20.910152859410942 76-77 19.27538572539547 28.146148188069326 31.40679831683464 21.171667769700566 78-79 19.32297861321653 28.050260916125012 31.252002405080514 21.374758065577943 80-81 19.291113494031137 28.331660312701167 31.22462475596294 21.15260143730476 82-83 19.302768560945207 28.27768122048419 31.051157809729464 21.368392408841135 84-85 19.309275943839175 28.071722825734003 30.839656603846443 21.77934462658038 86-87 19.301909334063673 28.045818399516147 30.839726016574893 21.812546249845287 88-89 19.318151888198823 27.831156904783228 30.848570089076414 22.00212111794153 90-91 19.202449732806702 27.993096079848083 30.90566544607278 21.898788741272433 92-93 19.36193020546448 28.1315049924102 30.596674887118763 21.909889915006552 94-95 19.235511822149515 28.25336199096214 30.306232332159354 22.20489385472899 96-97 19.273023454621892 28.356192878564862 30.205845618857786 22.164938047955456 98-99 19.44086935298941 28.701009785313076 29.697801860140082 22.160319001557433 100-101 19.626279854488736 28.830780796869153 29.362483598365017 22.18045575027709 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 879.0 1 648.5 2 558.5 3 656.0 4 501.0 5 483.0 6 908.0 7 2173.0 8 3951.5 9 4505.0 10 3415.0 11 2468.0 12 2318.5 13 2386.0 14 2479.5 15 2542.5 16 2580.0 17 2685.5 18 2765.5 19 2847.0 20 3036.0 21 3317.0 22 3680.0 23 4144.0 24 4624.0 25 5221.0 26 6230.0 27 7236.0 28 7973.0 29 8839.5 30 9934.5 31 10939.5 32 11943.0 33 12879.5 34 14021.0 35 14966.5 36 15920.0 37 17156.0 38 17325.5 39 16799.5 40 16856.0 41 17123.0 42 17200.0 43 17752.0 44 18469.0 45 18660.5 46 18399.0 47 18344.5 48 19295.5 49 18908.0 50 17027.0 51 15716.0 52 14631.5 53 13829.0 54 12981.5 55 12107.0 56 10907.5 57 9455.0 58 8558.0 59 7688.0 60 6633.0 61 5659.5 62 4572.5 63 3302.5 64 2271.0 65 1688.0 66 1255.0 67 906.0 68 606.5 69 380.5 70 273.0 71 217.5 72 162.5 73 121.5 74 88.0 75 78.5 76 58.5 77 33.5 78 17.5 79 7.5 80 9.5 81 7.0 82 4.5 83 3.5 84 3.5 85 3.0 86 0.5 87 0.5 88 1.5 89 1.5 90 0.5 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04054210587281362 2 0.0351013903660724 3 0.029309660955670454 4 0.05651323848937656 5 0.10951633794214588 6 0.12987514435446787 7 0.06809669731018045 8 0.10003896254330634 9 0.08248826736027014 10-11 0.08722695505968991 12-13 0.0808209513178817 14-15 0.07336190586509131 16-17 0.05695200586895247 18-19 0.0669559021232831 20-21 0.06458655827357322 22-23 0.03536465079381794 24-25 0.008161073260111833 26-27 0.002544850801540249 28-29 0.005967236362232308 30-31 0.003773399464352783 32-33 0.006581510693638575 34-35 0.007283538500960023 36-37 0.005616222458571584 38-39 0.00263260427745543 40-41 0.005177455078995679 42-43 0.002106083421964344 44-45 0.001140795186897353 46-47 0.001053041710982172 48-49 0.0 50-51 0.0012285486628125341 52-53 0.004124413368013507 54-55 0.010267156682076178 56-57 0.007722305880535928 58-59 0.004299920319843869 60-61 0.002106083421964344 62-63 0.007195785025044842 64-65 0.002106083421964344 66-67 0.020709820315982716 68-69 0.04642158875913075 70-71 0.05940910319457754 72-73 0.00263260427745543 74-75 0.012548747055870883 76-77 0.024044452400759593 78-79 0.026413796250469482 80-81 0.07871486789591736 82-83 0.029485167907500814 84-85 0.010003896254330635 86-87 0.031854511757210706 88-89 0.046860356138706656 90-91 0.04326246362618423 92-93 0.04624608180730039 94-95 0.011144691441227986 96-97 0.018164969514442467 98-99 0.04405224490942086 100-101 0.08336580211942195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 569778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.970588974610806 #Duplication Level Percentage of deduplicated Percentage of total 1 82.29378477903 51.820880964853814 2 10.708806547335966 13.486797110018284 3 3.1216393057153677 5.8971439694157555 4 1.2871791484915154 3.2421771638539494 5 0.6742139542295825 2.1227824896369047 6 0.4172148105334883 1.5763357408934648 7 0.29841608621742927 1.315400569602683 8 0.19692157460985873 0.9920214027992454 9 0.14936971890783063 0.8465309257138339 >10 0.7854730781544729 9.096048322903085 >50 0.043079069134162734 1.839920273682019 >100 0.0211226337286385 2.4916711082815852 >500 0.0011117175646646613 0.5089400563939713 >1k 8.337881734984959E-4 0.719656640953236 >5k 5.558587823323306E-4 2.1650954049689886 >10k+ 2.779293911661653E-4 1.8785978560291916 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10734 1.8838916209471055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7247 1.2718988799146336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.8992976211787749 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1610 0.2825661924468828 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1276 0.2239468705355419 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1226 0.21517152294402384 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 833 0.14619729087469155 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.13987904060879852 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.12127530371478014 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.10302258072442248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.75506951830362E-4 0.0 5 0.0 0.0 0.0 1.75506951830362E-4 0.0 6 0.0 0.0 0.0 1.75506951830362E-4 0.0 7 0.0 0.0 0.0 1.75506951830362E-4 0.0 8 0.0 0.0 0.0 1.75506951830362E-4 0.0 9 0.0 0.0 0.0 1.75506951830362E-4 0.0 10-11 0.0 0.0 0.0 3.51013903660724E-4 0.0 12-13 0.0 0.0 0.0 3.51013903660724E-4 0.0 14-15 0.0 0.0 0.0 3.51013903660724E-4 0.0 16-17 0.0 0.0 0.0 3.51013903660724E-4 0.0 18-19 0.0 0.0 0.0 5.26520855491086E-4 0.0 20-21 0.0 0.0 0.0 7.897812832366291E-4 0.0 22-23 0.0 0.0 0.0 0.001228548662812534 0.0 24-25 0.0 0.0 0.0 0.001930576470133982 0.0 26-27 0.0 0.0 0.0 0.003773399464352783 0.0 28-29 0.0 0.0 0.0 0.00956512887475473 0.0 30-31 0.0 0.0 0.0 0.02211387593062561 0.0 32-33 0.0 0.0 0.0 0.03913805025817073 0.0 34-35 0.0 0.0 0.0 0.05914584276683199 0.0 36-37 0.0 0.0 0.0 0.09082484757221235 0.0 38-39 0.0 0.0 0.0 0.13610564114444573 0.0 40-41 0.0 0.0 0.0 0.1888454801694695 0.0 42-43 0.0 0.0 0.0 0.2687888967281994 0.0 44-45 0.0 0.0 0.0 0.3589117164930903 0.0 46-47 0.0 0.0 0.0 0.4691300822425576 0.0 48-49 0.0 0.0 0.0 0.5967236362232309 0.0 50-51 0.0 0.0 0.0 0.7526615629245075 0.0 52-53 0.0 0.0 0.0 0.9249216361460078 0.0 54-55 0.0 0.0 0.0 1.085159483167128 0.0 56-57 0.0 0.0 0.0 1.3046309264309959 0.0 58-59 0.0 0.0 0.0 1.545689724769998 0.0 60-61 0.0 0.0 0.0 1.8150051423536886 0.0 62-63 0.0 0.0 0.0 2.1483805973554615 0.0 64-65 0.0 0.0 0.0 2.452270884449733 0.0 66-67 0.0 0.0 0.0 2.800739937308917 0.0 68-69 0.0 0.0 0.0 3.2186219896170085 0.0 70-71 0.0 0.0 0.0 3.691964238703495 0.0 72-73 0.0 0.0 0.0 4.227348195262015 0.0 74-75 0.0 0.0 0.0 4.736581615997809 0.0 76-77 0.0 0.0 0.0 5.246166050637266 0.0 78-79 0.0 0.0 0.0 5.8448202633306305 0.0 80-81 0.0 0.0 0.0 6.577649540698307 0.0 82-83 0.0 0.0 0.0 7.275640688127657 0.0 84-85 0.0 0.0 0.0 8.048573303988572 0.0 86-87 0.0 0.0 0.0 8.923475458862926 0.0 88-89 0.0 0.0 0.0 9.850415424954981 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3625 0.0 50.606625 1 GTATCAA 6590 0.0 42.261047 1 TATCAAC 8385 0.0 32.99609 2 ATCAACG 8430 0.0 32.876343 3 TCAACGC 8620 0.0 32.15169 4 CAACGCA 8735 0.0 31.782824 5 AACGCAG 8910 0.0 31.155842 6 GTACATG 4610 0.0 28.041162 1 ACGCAGA 9845 0.0 28.003778 7 CGCAGAG 9850 0.0 27.796532 8 TACATGG 4640 0.0 27.25249 2 GCAGAGT 10000 0.0 26.802113 9 ATGGGAT 1410 0.0 25.960566 5 GAGTACT 6240 0.0 25.362246 12-13 ACATGGG 4820 0.0 24.952612 3 CATGGGA 3740 0.0 24.15041 4 AGTACTT 6410 0.0 24.022326 12-13 GTGTAAG 180 1.9373147E-7 23.762823 1 GTACTTT 6755 0.0 23.530035 14-15 AGAGTAC 9060 0.0 22.580568 10-11 >>END_MODULE