FastQCFastQC Report
Thu 26 May 2016
SRR936780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569778
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83291.4617974017950852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65401.1478154649705676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47880.8403272853637733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.25518710796134636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14390.25255450368389093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.22219180101723832No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.21359196037755057No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7480.13127919996911078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.11723864382268182No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC201.7407303E-595.025739
TATAACG250.005019832457.0004232
GGTATCA37900.046.7524721
GTATCAA64050.038.4189571
TATCAAC82950.029.6626682
ATCAACG83350.029.5177233
TCAACGC83900.029.2676124
CAACGCA85600.028.6863635
AACGCAG88350.027.7983446
ATAACGC700.00839136927.1406763
GGATACG908.9278625E-426.3960348
ACGCAGA100300.024.4390167
GTAAGGT1951.567605E-824.3570164
CGCAGAG100450.024.2649068
GAGTACT58650.023.4505812-13
GCAGAGT103350.023.3081979
AGTACTT59550.022.77704612-13
GTACATG52800.022.40271
TACATGG53350.021.9026932
GTACTTT63050.021.81023214-15
Produced by FastQC (version 0.11.5)