##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936780_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569778 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76983667323063 31.0 31.0 34.0 30.0 34.0 2 31.89673697475157 31.0 31.0 34.0 30.0 34.0 3 31.95986682532495 31.0 31.0 34.0 30.0 34.0 4 35.77471752156103 37.0 35.0 37.0 35.0 37.0 5 35.5913127568983 37.0 35.0 37.0 33.0 37.0 6 35.71885892400198 37.0 35.0 37.0 33.0 37.0 7 35.65563780981365 37.0 35.0 37.0 33.0 37.0 8 35.62218091958623 37.0 35.0 37.0 33.0 37.0 9 37.205894225470274 39.0 37.0 39.0 33.0 39.0 10-11 37.11403739702129 39.0 37.0 39.0 33.0 39.0 12-13 36.980559972480506 39.0 37.0 39.0 33.0 39.0 14-15 37.93731856968854 40.0 37.0 41.0 33.0 41.0 16-17 37.96496968994942 39.5 37.0 41.0 33.0 41.0 18-19 38.20549933482865 40.0 37.5 41.0 33.5 41.0 20-21 38.25564079343183 40.0 38.0 41.0 33.5 41.0 22-23 38.13655581647589 40.0 37.0 41.0 33.0 41.0 24-25 38.01682585147198 40.0 37.0 41.0 33.0 41.0 26-27 37.74278139907122 40.0 37.0 41.0 33.0 41.0 28-29 37.529887078827194 39.0 36.0 41.0 32.0 41.0 30-31 37.32111366181214 39.0 36.0 41.0 32.0 41.0 32-33 37.020769492679605 39.0 35.0 41.0 31.5 41.0 34-35 36.7668662180709 39.0 35.0 40.5 31.0 41.0 36-37 36.515234880953635 39.0 35.0 40.0 31.0 41.0 38-39 36.252994148598226 38.0 35.0 40.0 30.0 41.0 40-41 35.74649688124147 38.0 35.0 40.0 29.0 41.0 42-43 35.39740477870328 38.0 35.0 40.0 27.5 41.0 44-45 34.91745995808894 38.0 34.5 40.0 26.0 41.0 46-47 34.20380042753494 38.0 33.5 40.0 23.0 41.0 48-49 33.53485480309875 38.0 33.0 40.0 17.0 41.0 50-51 33.11636198659829 38.0 33.0 40.0 10.5 41.0 52-53 32.79053508559474 37.5 32.5 40.0 8.0 41.0 54-55 32.38783526215474 37.0 32.0 40.0 6.0 41.0 56-57 32.10958039797956 37.0 31.0 40.0 2.0 41.0 58-59 31.74576764985661 36.0 31.0 39.0 2.0 40.5 60-61 31.310957074509723 36.0 30.5 39.0 2.0 40.0 62-63 30.90421708103858 35.0 30.0 39.0 2.0 40.0 64-65 30.556484279842323 35.0 29.0 38.0 2.0 40.0 66-67 30.192447760355787 35.0 29.0 38.0 2.0 40.0 68-69 29.687957414993207 34.5 28.5 38.0 2.0 40.0 70-71 29.2153417646873 34.0 28.0 37.0 2.0 39.0 72-73 28.78310763139328 34.0 27.0 36.5 2.0 39.0 74-75 28.273189733545344 34.0 26.0 36.0 2.0 39.0 76-77 26.970776337450726 31.5 25.5 34.5 2.0 37.0 78-79 27.607003955926693 33.0 26.0 35.0 2.0 37.0 80-81 27.438662952939566 33.0 26.0 35.0 2.0 37.0 82-83 27.052306336854002 33.0 25.5 35.0 2.0 36.5 84-85 26.56440315350891 33.0 25.0 35.0 2.0 36.0 86-87 26.0984085029608 32.0 24.0 35.0 2.0 35.5 88-89 25.643659460351223 32.0 22.0 34.5 2.0 35.0 90-91 25.10384044311995 32.0 19.5 34.0 2.0 35.0 92-93 24.476544373422634 31.0 16.5 34.0 2.0 35.0 94-95 23.808946642376505 31.0 4.5 34.0 2.0 35.0 96-97 22.91898248089607 31.0 2.0 34.0 2.0 35.0 98-99 21.89595158114213 30.0 2.0 34.0 2.0 35.0 100-101 18.917337103222657 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08953372570214313 1101 2 0.3425010207325485 1101 3 0.19855757952784003 1101 4 0.1524457337556555 1101 5 0.021682941409352452 1101 6 0.16702017351929044 1101 7 0.18764392841015365 1101 8 0.24651094806245055 1101 9 0.31223664586320865 1101 10-11 0.3942796751753619 1101 12-13 0.2537756612338029 1101 14-15 0.3484254033143088 1101 16-17 0.35147323357829663 1101 18-19 0.2605001069520583 1101 20-21 -0.07202706356327582 1101 22-23 0.13559994264895892 1101 24-25 0.24858390044580858 1101 26-27 0.20289206568233453 1101 28-29 0.25426310982167877 1101 30-31 0.1739175236458621 1101 32-33 0.2598156896246806 1101 34-35 0.01939559590462636 1101 36-37 0.30579870199633774 1101 38-39 0.4017368014759626 1101 40-41 0.5068720098672159 1101 42-43 0.6533064620610389 1101 44-45 0.36582166479757916 1101 46-47 0.21571753129003923 1101 48-49 0.29108825170909824 1101 50-51 0.1961524964901784 1101 52-53 0.3788263784391219 1101 54-55 0.14261415807408895 1101 56-57 0.29373580176927305 1101 58-59 0.3197423961312147 1101 60-61 0.557644198052099 1101 62-63 0.5949445337842043 1101 64-65 0.43395099963467487 1101 66-67 0.2986732131792671 1101 68-69 0.19277587341103697 1101 70-71 0.1663982158440369 1101 72-73 0.2515225441685196 1101 74-75 0.22860004934964095 1101 76-77 0.49208864093125015 1101 78-79 0.597945806569605 1101 80-81 0.6274901918695228 1101 82-83 0.8480420846738959 1101 84-85 0.5951397759209804 1101 86-87 0.17494342755439973 1101 88-89 0.2534382866148306 1101 90-91 0.5215205608673514 1101 92-93 0.07627447102025897 1101 94-95 -0.15714984559344813 1101 96-97 -0.22871799217089617 1101 98-99 0.2929827949251802 1101 100-101 0.7881154137999005 1102 1 -0.03273101396689526 1102 2 0.09844113063971704 1102 3 0.1406754658329099 1102 4 0.1394004669047746 1102 5 0.04292079418342354 1102 6 0.2471802219529593 1102 7 0.33549313204888875 1102 8 0.2812046672092734 1102 9 0.16968254607743205 1102 10-11 0.3725699046143447 1102 12-13 0.2834226543102716 1102 14-15 0.3752222613353595 1102 16-17 0.27943397436503403 1102 18-19 0.4215071236244228 1102 20-21 0.28915381755690817 1102 22-23 0.24017281048092798 1102 24-25 0.19669157221456857 1102 26-27 0.43108915345283805 1102 28-29 0.2083124438587589 1102 30-31 0.13578338411964097 1102 32-33 0.19281190186350017 1102 34-35 0.33848265231743113 1102 36-37 0.34206418679871575 1102 38-39 0.5619664733068177 1102 40-41 0.5754700725205097 1102 42-43 0.12086677129137513 1102 44-45 0.24511898852697556 1102 46-47 0.20511645705612835 1102 48-49 0.39430008691043383 1102 50-51 0.18315690519582262 1102 52-53 0.35896003673338583 1102 54-55 0.5860717320440969 1102 56-57 0.4192259597996326 1102 58-59 0.13517441823683995 1102 60-61 0.30625638719178383 1102 62-63 0.36383715696405616 1102 64-65 0.6461999052200724 1102 66-67 0.4708886653820663 1102 68-69 0.7568662674002944 1102 70-71 0.47867467583968804 1102 72-73 0.32943826648216046 1102 74-75 0.39710729887616836 1102 76-77 0.5744623563524058 1102 78-79 0.10518679511318041 1102 80-81 0.4169139554138752 1102 82-83 0.5090425038114041 1102 84-85 0.25059283252006637 1102 86-87 0.1429155005774696 1102 88-89 0.4063237378862148 1102 90-91 0.4516452930850541 1102 92-93 0.32152075311532613 1102 94-95 0.25439894511722727 1102 96-97 0.32956695924019996 1102 98-99 0.3697369630148444 1102 100-101 0.16776861632093798 1103 1 0.15833077580944277 1103 2 0.2659596507015465 1103 3 0.2849688272711397 1103 4 0.22751215743654996 1103 5 0.06993889399306852 1103 6 0.14179194856442479 1103 7 0.08848444344916118 1103 8 0.1553180468804456 1103 9 0.2994688221754984 1103 10-11 0.3449984134660653 1103 12-13 0.4162990798392485 1103 14-15 0.280342833569442 1103 16-17 0.26813493266199373 1103 18-19 0.31024548284197095 1103 20-21 0.46868128538246623 1103 22-23 0.3231169743620086 1103 24-25 0.34177462156853977 1103 26-27 0.3874647400019242 1103 28-29 0.31236424413377506 1103 30-31 0.24916661244921556 1103 32-33 0.042708185451907354 1103 34-35 -0.10906865452967907 1103 36-37 -0.015965272524219642 1103 38-39 0.20486339819905908 1103 40-41 0.12468633013155284 1103 42-43 -0.2375219604616987 1103 44-45 -0.057731916971668795 1103 46-47 -0.2568733328990831 1103 48-49 -0.059797397369486305 1103 50-51 -0.2534327812970645 1103 52-53 -0.3652849067739439 1103 54-55 -0.11292200025271626 1103 56-57 -0.22471422718463785 1103 58-59 -0.19007638644193392 1103 60-61 -0.22015496398761414 1103 62-63 0.1556766419696416 1103 64-65 0.007160966593154683 1103 66-67 0.04568542491752581 1103 68-69 0.021070232507671705 1103 70-71 -0.19448802934450882 1103 72-73 -0.18407586506947737 1103 74-75 -0.5346131536343179 1103 76-77 -0.27347749321052106 1103 78-79 -0.47510576545750993 1103 80-81 -0.24354056740424568 1103 82-83 -0.12110965085766523 1103 84-85 -0.2864101951251037 1103 86-87 -0.387840150467877 1103 88-89 -0.22843188063659525 1103 90-91 -0.1523719519915936 1103 92-93 -0.2436224019572215 1103 94-95 -0.004512484827696284 1103 96-97 0.44147650999213184 1103 98-99 0.6667871686690887 1103 100-101 1.4638060497640666 1104 1 -0.07681162299597233 1104 2 0.029343598187789866 1104 3 -0.026055642689954084 1104 4 -0.029197274338770285 1104 5 -0.16231362942680505 1104 6 0.007369669113209909 1104 7 -0.0251905147494611 1104 8 0.007982697334746547 1104 9 0.024774027736484072 1104 10-11 0.11852107236025944 1104 12-13 0.10954742246830307 1104 14-15 0.053062749300984535 1104 16-17 -0.13094980960524083 1104 18-19 0.09034280402929795 1104 20-21 0.1000701093702574 1104 22-23 0.10620532962611406 1104 24-25 0.17380420428567334 1104 26-27 0.09174329579517604 1104 28-29 0.07771001759312668 1104 30-31 0.09336656467620941 1104 32-33 -0.02396257107151456 1104 34-35 0.23300957572838854 1104 36-37 0.12681728327948605 1104 38-39 0.017230341888257783 1104 40-41 0.2809947819075731 1104 42-43 0.11254128003623975 1104 44-45 0.5269490024107242 1104 46-47 0.16091271451106337 1104 48-49 0.37968229453377234 1104 50-51 0.5964744790253675 1104 52-53 0.5871430976065497 1104 54-55 0.7314763535364293 1104 56-57 0.5817372172962081 1104 58-59 0.2871753081293029 1104 60-61 0.6152441821025043 1104 62-63 0.8232685299562981 1104 64-65 0.5897417010183048 1104 66-67 0.24354790914003033 1104 68-69 0.5479699600971095 1104 70-71 0.6588814687648075 1104 72-73 0.5556500014036594 1104 74-75 0.24062006609671727 1104 76-77 0.31117732070317317 1104 78-79 0.3235518913417259 1104 80-81 0.48536131910745084 1104 82-83 0.40442541335525917 1104 84-85 0.4368046150293985 1104 86-87 0.4710193277099073 1104 88-89 0.4875549778704631 1104 90-91 0.42660100665865386 1104 92-93 0.34132740375282467 1104 94-95 0.6107366331337793 1104 96-97 0.623999420804104 1104 98-99 0.6311910568175989 1104 100-101 1.1410302030658244 1105 1 -0.038302255196406065 1105 2 0.10077750450605905 1105 3 -0.09112422536447085 1105 4 0.09279511490758807 1105 5 0.15046668568378863 1105 6 -0.011532111833012948 1105 7 0.15325887822011452 1105 8 0.2528992143133024 1105 9 0.39924327289760697 1105 10-11 0.1559988058643782 1105 12-13 0.16626957020953625 1105 14-15 0.2594867033825281 1105 16-17 0.1038548240665591 1105 18-19 -0.018505350605678927 1105 20-21 0.14364148726023274 1105 22-23 0.038283647940843935 1105 24-25 0.20393912898104105 1105 26-27 0.1600356189422527 1105 28-29 -0.004408072324309842 1105 30-31 0.11935534647015089 1105 32-33 0.04822568865011334 1105 34-35 0.15578824769295352 1105 36-37 0.22370874974996724 1105 38-39 0.05231057596187583 1105 40-41 -0.026942920451467955 1105 42-43 0.4076773810747838 1105 44-45 0.3640516131456977 1105 46-47 0.040461708270839836 1105 48-49 0.37238149798139375 1105 50-51 0.14289988953179034 1105 52-53 0.1430184649803934 1105 54-55 0.303117520319482 1105 56-57 0.2269745211923322 1105 58-59 0.3554601624307985 1105 60-61 0.4595738345397713 1105 62-63 0.5183547764506109 1105 64-65 0.5008475009503357 1105 66-67 0.37563817760768714 1105 68-69 0.25293935650235255 1105 70-71 0.4300920566002979 1105 72-73 0.6135279529980728 1105 74-75 0.48606033587141795 1105 76-77 0.47728257914390326 1105 78-79 0.851139939695031 1105 80-81 0.8623064865776406 1105 82-83 0.6184311793040465 1105 84-85 0.44014879063689705 1105 86-87 0.6874141906379201 1105 88-89 0.8788244575533319 1105 90-91 0.789256405326956 1105 92-93 0.8282223805807298 1105 94-95 0.736176828220021 1105 96-97 0.65584004310832 1105 98-99 0.8273434810181541 1105 100-101 0.8343584700322388 1106 1 0.0794803906552346 1106 2 0.1273840691839041 1106 3 0.2222145644912139 1106 4 0.11818599850311529 1106 5 -0.017971344672126577 1106 6 0.22316655573236943 1106 7 0.06281100205107748 1106 8 0.2012147136452782 1106 9 0.5851160958053754 1106 10-11 0.28834856092269945 1106 12-13 0.44608384784220334 1106 14-15 0.7502246579088307 1106 16-17 0.31699019797876105 1106 18-19 0.38238354095797433 1106 20-21 0.17170446194758426 1106 22-23 0.3202404130255161 1106 24-25 0.2617441626229109 1106 26-27 0.4422571858468345 1106 28-29 0.7611099161447754 1106 30-31 0.4173807674448611 1106 32-33 0.2734546820421073 1106 34-35 0.4411633635437511 1106 36-37 0.3348984321081474 1106 38-39 0.3191044277450814 1106 40-41 0.4075306655670019 1106 42-43 0.5247047399173539 1106 44-45 0.13186555375786924 1106 46-47 0.003816747329111081 1106 48-49 0.17444514670974343 1106 50-51 0.45515840832670307 1106 52-53 0.48451652109431365 1106 54-55 0.5748433059985203 1106 56-57 0.5354415593839761 1106 58-59 0.6649743714557239 1106 60-61 0.4988728319037925 1106 62-63 0.3516740672631897 1106 64-65 0.42054333995083226 1106 66-67 0.7653211376105737 1106 68-69 0.42993530021992754 1106 70-71 0.4619916485647657 1106 72-73 0.4740887310339623 1106 74-75 0.17610679655436812 1106 76-77 0.48528681597301926 1106 78-79 0.7406363347793494 1106 80-81 0.5205887653176653 1106 82-83 0.666923012575193 1106 84-85 0.5968603131269958 1106 86-87 0.13689722526677528 1106 88-89 0.6312287377136592 1106 90-91 0.5611025554695885 1106 92-93 0.8149311346881696 1106 94-95 1.0652412352444962 1106 96-97 1.113905063991556 1106 98-99 0.8318691848493529 1106 100-101 1.6726096947406575 1107 1 0.12315716869160198 1107 2 0.22837614050258992 1107 3 0.2856144303742134 1107 4 0.06938121762662064 1107 5 0.043544966321519496 1107 6 0.1492542840271156 1107 7 0.13635839663117366 1107 8 0.04699002822852805 1107 9 0.1870450137967694 1107 10-11 0.18979229095307204 1107 12-13 0.4250787592855403 1107 14-15 0.44021716313545056 1107 16-17 -0.019993550196858223 1107 18-19 0.21674411844998787 1107 20-21 0.08115083487173536 1107 22-23 0.09334107966589045 1107 24-25 0.13736543989006833 1107 26-27 0.0845592646602995 1107 28-29 0.3388287438044344 1107 30-31 0.08996271687485802 1107 32-33 -0.08667904049349318 1107 34-35 0.06942753458236695 1107 36-37 0.058050378776918876 1107 38-39 0.3266928857940741 1107 40-41 0.4912699657918438 1107 42-43 0.2676389436962978 1107 44-45 0.2041999784353976 1107 46-47 -0.1526711624180379 1107 48-49 -0.4022924573295512 1107 50-51 -0.500765421108035 1107 52-53 0.1386475218437866 1107 54-55 -0.11591346888215526 1107 56-57 -0.5130870982626661 1107 58-59 -0.5838027970977855 1107 60-61 -0.46880102711557825 1107 62-63 -0.7270013301570302 1107 64-65 -0.6412885404483646 1107 66-67 -0.7303151292033583 1107 68-69 -0.9156279911689715 1107 70-71 -0.9994792993268398 1107 72-73 -1.004334210861817 1107 74-75 -0.8111077117490524 1107 76-77 -1.3999346743034984 1107 78-79 -1.2555275246344841 1107 80-81 -1.0355727273256221 1107 82-83 -0.9147453634257552 1107 84-85 -0.7393739577104448 1107 86-87 -0.6054502166487268 1107 88-89 -0.5888365457071139 1107 90-91 -0.580647276884168 1107 92-93 -0.6278176720650173 1107 94-95 -0.5612379963440048 1107 96-97 -1.1140741084276833 1107 98-99 -0.9670539345542224 1107 100-101 -0.37050004452012075 1108 1 -0.054580381182788074 1108 2 0.0959108150787884 1108 3 -0.11773480646844803 1108 4 -0.06014969921773172 1108 5 -0.20181592371204005 1108 6 -0.008533509677633333 1108 7 0.04030401507361603 1108 8 0.11905720296306299 1108 9 -0.02834902027614561 1108 10-11 -0.02832504316840101 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-1.3722567652956066 2316 94-95 -1.3964324320771695 2316 96-97 -0.7908695028992554 2316 98-99 -1.3624215256119712 2316 100-101 -2.187326127580917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 16.0 6 53.0 7 143.0 8 321.0 9 717.0 10 1183.0 11 1951.0 12 2954.0 13 4036.0 14 5092.0 15 7335.0 16 9641.0 17 12934.0 18 11011.0 19 6265.0 20 5244.0 21 5220.0 22 5463.0 23 5824.0 24 6708.0 25 7597.0 26 8859.0 27 10743.0 28 12679.0 29 15228.0 30 18288.0 31 22034.0 32 27057.0 33 34167.0 34 44318.0 35 58919.0 36 79364.0 37 93634.0 38 43366.0 39 1400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.889851439525195 20.975938574640974 16.52807692915335 18.60613305668048 2 15.603505317123084 22.950946270642355 40.34782883409417 21.097719578140385 3 18.708404706410942 29.092836483109153 32.142806997837745 20.05595181264216 4 12.147187150083015 19.36771865533594 38.26806229794762 30.21703189663343 5 11.555607819460192 40.198645740823295 33.23200376300113 15.013742676715378 6 27.224989381829413 39.58980515218208 17.551572717795352 15.633632748193154 7 24.697162754616713 37.72557030983997 20.908494185454686 16.668772750088632 8 26.875028519879674 34.05185879412684 19.508299723752057 19.564812962241433 9 27.75765298063456 16.496249416439383 21.093829526587548 34.65226807633851 10-11 24.070251922678658 29.010684863227432 26.319110249957 20.599952964136907 12-13 26.49321449633519 27.342543317700578 27.86163610323233 18.30260608273189 14-15 23.019347116010735 26.9955717342198 29.00048968931933 20.984591460450126 16-17 19.130521009937205 30.740393626991562 28.975144705481785 21.153940657589448 18-19 19.1134258548293 26.966842320028434 35.11335565198696 18.80637617315531 20-21 20.981799673552487 25.675184723660426 35.3673412078558 17.97567439493129 22-23 20.234446095212355 26.346840866071048 35.19025709564498 18.228455943071616 24-25 19.562231857579242 26.342785285107407 35.651435255753796 18.44354760155956 26-27 19.484974397900935 26.61416079083502 35.498505995761505 18.40235881550254 28-29 18.995079649652098 26.85728526887297 35.55815306528086 18.58948201619407 30-31 19.24565357033792 26.551218193752657 35.523923352604 18.679204883305427 32-33 19.14563140875166 26.91357591340479 35.177327992067084 18.763464685776466 34-35 19.331651976734797 26.734622958415382 35.27198312325151 18.661741941598304 36-37 19.71797367180939 26.625854841299855 35.0153000467733 18.64087144011745 38-39 19.46354490746795 26.96896641604714 34.8798491894724 18.687639487012515 40-41 19.410927433013267 27.14360179508825 34.52447185477827 18.920998917120208 42-43 19.413438216287748 27.449901540600024 34.04088960963744 19.095770633474793 44-45 19.60231635497903 27.502371552254445 33.691047949671315 19.204264143095205 46-47 19.998771450259092 27.99768330620286 32.73751596017744 19.266029283360613 48-49 20.103022671130397 28.406439355713413 31.886920771704748 19.603617201451442 50-51 19.98251912409579 28.951559896132707 31.40046211994233 19.665458859829172 52-53 20.0820848174679 29.249714581068194 30.966352596149544 19.701848005314364 54-55 19.916441140233566 29.116076787801564 30.88580113658302 20.08168093538185 56-57 19.988311237008098 29.484992400548986 30.60955319440203 19.917143168040884 58-59 20.055372491893767 28.896016427465103 30.943921092005212 20.104689988635915 60-61 19.828474346621174 28.893814861812416 31.354724458508628 19.922986333057782 62-63 19.98567863273064 28.70267016276515 31.442158173885264 19.869493030618944 64-65 19.75260540069992 28.953381843454817 31.273408239700373 20.020604516144886 66-67 19.787180270210502 29.021390787289086 30.97434439378144 20.217084548718976 68-69 19.914966807218608 29.00904300362861 30.73813901040318 20.3378511787496 70-71 19.93871248838354 29.095772267400992 30.671135108951187 20.294380135264277 72-73 20.051967927662613 29.00015707968679 30.440112711256823 20.50776228139378 74-75 19.829924659607983 29.41443358680819 30.03830644276631 20.71733531081751 76-77 19.652671437534828 29.374185537336945 30.162738963893798 20.81040406123443 78-79 19.70221630956727 29.397780882311647 29.867877902895174 21.032124905225913 80-81 19.55737270450385 29.714509869997208 29.800862472948996 20.927254952549948 82-83 19.604424187006494 29.584820026080376 29.69591612157037 21.114839665342757 84-85 19.539926266747287 29.491896977041947 29.48004981075249 21.48812694545828 86-87 19.407819395533853 29.25704948703481 29.47792595504721 21.857205162384133 88-89 19.351277445127295 29.163467166768616 29.541176357019435 21.94407903108466 90-91 19.278822316316006 29.33766262258397 29.482459796845177 21.901055264254843 92-93 19.28505488102384 29.628469333670303 29.157408674957612 21.929067110348242 94-95 18.950801891262913 29.7480773213427 28.869489520479906 22.431631266914483 96-97 19.161406553984857 29.819814997310345 28.703410378298116 22.315368070406677 98-99 19.231899917422748 30.133150335354912 28.154630951639213 22.480318795583127 100-101 19.030957853195694 30.384452360078875 27.876021156332026 22.7085686303934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1114.0 1 825.0 2 532.5 3 578.5 4 574.5 5 580.5 6 898.0 7 1815.0 8 2839.5 9 3284.0 10 2948.0 11 2456.0 12 2417.0 13 2495.0 14 2550.5 15 2606.0 16 2729.0 17 2830.5 18 2853.0 19 3032.5 20 3265.5 21 3472.5 22 3863.5 23 4355.0 24 4857.0 25 5486.0 26 6406.5 27 7471.0 28 8260.5 29 9128.0 30 10123.5 31 11084.0 32 12228.0 33 13237.0 34 14171.5 35 14806.0 36 15853.5 37 16792.5 38 16935.5 39 16846.0 40 16765.0 41 17131.0 42 17441.5 43 17762.5 44 18442.5 45 18660.0 46 18421.5 47 18493.5 48 19359.5 49 18788.5 50 16816.5 51 15568.5 52 14364.5 53 13451.5 54 12670.0 55 11853.0 56 10846.0 57 9586.5 58 8684.0 59 7887.0 60 6750.5 61 5619.5 62 4616.0 63 3455.0 64 2429.0 65 1726.5 66 1165.5 67 863.5 68 639.0 69 411.0 70 284.5 71 203.5 72 145.0 73 106.5 74 84.5 75 79.0 76 62.5 77 36.0 78 19.5 79 12.5 80 10.5 81 9.0 82 5.5 83 3.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019481271653170183 2 0.00263260427745543 3 3.51013903660724E-4 4 0.0 5 0.00351013903660724 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005967236362232308 14-15 0.005089701603080498 16-17 0.0 18-19 8.7753475915181E-5 20-21 0.001404055614642896 22-23 0.001667316042388439 24-25 2.63260427745543E-4 26-27 8.7753475915181E-5 28-29 2.63260427745543E-4 30-31 0.0 32-33 8.7753475915181E-5 34-35 0.0 36-37 0.001491809090558077 38-39 0.009916142778415453 40-41 1.75506951830362E-4 42-43 0.0 44-45 6.142743314062671E-4 46-47 8.7753475915181E-5 48-49 8.7753475915181E-5 50-51 0.002193836897879525 52-53 2.63260427745543E-4 54-55 0.0 56-57 0.0 58-59 8.7753475915181E-5 60-61 0.001579562566473258 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.7753475915181E-5 70-71 7.89781283236629E-4 72-73 6.142743314062671E-4 74-75 0.005352962030826041 76-77 8.7753475915181E-5 78-79 3.51013903660724E-4 80-81 0.0036856459884376017 82-83 1.75506951830362E-4 84-85 0.003773399464352783 86-87 4.38767379575905E-4 88-89 0.004826441175334956 90-91 0.002720357753370611 92-93 0.0 94-95 0.0 96-97 4.38767379575905E-4 98-99 0.001491809090558077 100-101 0.0034223855606920588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 569778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.30287929649659 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8297453349832 50.982287464685136 2 10.862604218973972 13.535430390007 3 3.2151172951843296 6.009331942978437 4 1.3668404367609202 3.4063237899634515 5 0.7289013085558118 2.270632512300558 6 0.4361509359625603 1.6304075470997648 7 0.2870877849756771 1.2520476930386724 8 0.22440729090784403 1.1184976286948163 9 0.143597281549897 0.8051871689737444 >10 0.8287032759217999 9.354479435081856 >50 0.04848881291342611 2.0712348462173678 >100 0.02523686074639077 2.704759365295623 >500 0.001134240932421748 0.43689548728771926 >1k 0.0014178011655271848 1.795635152766833 >5k 5.67120466210874E-4 2.626849575609016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8329 1.4617974017950852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6540 1.1478154649705676 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4788 0.8403272853637733 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1454 0.25518710796134636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1439 0.25255450368389093 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1266 0.22219180101723832 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.21359196037755057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 748 0.13127919996911078 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.11723864382268182 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.75506951830362E-4 0.0 12-13 0.0 0.0 0.0 1.75506951830362E-4 0.0 14-15 0.0 0.0 0.0 1.75506951830362E-4 0.0 16-17 0.0 0.0 0.0 1.75506951830362E-4 0.0 18-19 0.0 0.0 0.0 3.51013903660724E-4 0.0 20-21 0.0 0.0 0.0 6.14274331406267E-4 0.0 22-23 0.0 0.0 0.0 0.001053041710982172 0.0 24-25 0.0 0.0 0.0 0.00175506951830362 0.0 26-27 0.0 0.0 0.0 0.003597892512522421 0.0 28-29 0.0 0.0 0.0 0.009477375398839548 0.0 30-31 0.0 0.0 0.0 0.021938368978795253 0.0 32-33 0.0 0.0 0.0 0.03869928287859482 0.0 34-35 0.0 0.0 0.0 0.058882582339086456 0.0 36-37 0.0 0.0 0.0 0.0904738336685516 0.0 38-39 0.0 0.0 0.0 0.13549136681303947 0.0 40-41 0.0 0.0 0.0 0.1884944662658088 0.0 42-43 0.0 0.0 0.0 0.2685256363004539 0.0 44-45 0.0 0.0 0.0 0.3583851956375992 0.0 46-47 0.0 0.0 0.0 0.4678137801038299 0.0 48-49 0.0 0.0 0.0 0.5941787854216906 0.0 50-51 0.0 0.0 0.0 0.7480106287010029 0.0 52-53 0.0 0.0 0.0 0.9181646185005388 0.0 54-55 0.0 0.0 0.0 1.075418847340543 0.0 56-57 0.0 0.0 0.0 1.293486234989768 0.0 58-59 0.0 0.0 0.0 1.5334042381418729 0.0 60-61 0.0 0.0 0.0 1.8021931348700724 0.0 62-63 0.0 0.0 0.0 2.1317074369315767 0.0 64-65 0.0 0.0 0.0 2.434193668411206 0.0 66-67 0.0 0.0 0.0 2.7801178704688496 0.0 68-69 0.0 0.0 0.0 3.1947530441680794 0.0 70-71 0.0 0.0 0.0 3.6636198659828914 0.0 72-73 0.0 0.0 0.0 4.193914120938331 0.0 74-75 0.0 0.0 0.0 4.6983211004987915 0.0 76-77 0.0 0.0 0.0 5.203430107866573 0.0 78-79 0.0 0.0 0.0 5.792957959064759 0.0 80-81 0.0 0.0 0.0 6.515081312370783 0.0 82-83 0.0 0.0 0.0 7.204209358732699 0.0 84-85 0.0 0.0 0.0 7.963891199730421 0.0 86-87 0.0 0.0 0.0 8.824050770651025 0.0 88-89 0.0 0.0 0.0 9.733089027656385 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGC 20 1.7407303E-5 95.02573 9 TATAACG 25 0.0050198324 57.000423 2 GGTATCA 3790 0.0 46.752472 1 GTATCAA 6405 0.0 38.418957 1 TATCAAC 8295 0.0 29.662668 2 ATCAACG 8335 0.0 29.517723 3 TCAACGC 8390 0.0 29.267612 4 CAACGCA 8560 0.0 28.686363 5 AACGCAG 8835 0.0 27.798344 6 ATAACGC 70 0.008391369 27.140676 3 GGATACG 90 8.9278625E-4 26.396034 8 ACGCAGA 10030 0.0 24.439016 7 GTAAGGT 195 1.567605E-8 24.357016 4 CGCAGAG 10045 0.0 24.264906 8 GAGTACT 5865 0.0 23.45058 12-13 GCAGAGT 10335 0.0 23.308197 9 AGTACTT 5955 0.0 22.777046 12-13 GTACATG 5280 0.0 22.4027 1 TACATGG 5335 0.0 21.902693 2 GTACTTT 6305 0.0 21.810232 14-15 >>END_MODULE