FastQCFastQC Report
Thu 26 May 2016
SRR936779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571752
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146500.9320808880790353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97010.6172093307341108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.45293405066448145No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.14385221078134464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21690.13799887005074593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.1276919005033873No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.12616494205192677No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17060.10854129659131975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118150.035.9622041
GGTATCA71750.033.517461
TCAACGC143500.029.3434014
ATCAACG144050.029.2295023
CAACGCA145150.028.975255
TATCAAC145100.028.9561732
AACGCAG147950.028.425986
ACGCAGA160350.025.9906887
CGCAGAG162800.025.4519778
GCAGAGT168000.024.127549
GAGTACT81600.023.72579812-13
GTACATG85800.023.0988981
CCTATAC5050.022.5850373
AGTACTT85150.022.5134612-13
GGACCGT1703.1380441E-622.3629046
CCCTATA3251.8189894E-1221.9363422
GTACTTT88950.021.7406914-15
TACATGG90550.021.5729582
CATGGGG34900.021.3797634
ACATGGG90600.021.2435823
Produced by FastQC (version 0.11.5)