FastQCFastQC Report
Thu 26 May 2016
SRR936779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571752
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129080.8212491538105249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98070.6239533972280614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72900.46381362963113776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31270.19894996157154565No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.17884500862731526No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.13901684235171963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.1378716235131242No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16680.10612361237650723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.035.7086261
GTATCAA118500.033.074041
TATCAAC147000.026.5605492
ATCAACG147650.026.4741063
TCAACGC148150.026.351864
CAACGCA149900.026.0442185
AACGCAG153650.025.4394876
GTATAGG6700.024.10771
GTACATG90350.023.5034181
GAGTACT91300.022.78475812-13
ACGCAGA171600.022.5846827
CGCAGAG173150.022.300228
TACATGG95050.022.037842
ACATGGG93050.021.7954793
AGTACTT95400.021.40726512-13
GCAGAGT178900.021.1852519
GTACTTT97950.021.16546414-15
CATGGGG38850.019.9268054
CATGGGA53250.019.1760854
ACTTTTT110400.018.99400516-17
Produced by FastQC (version 0.11.5)