FastQCFastQC Report
Thu 26 May 2016
SRR936778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427592
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138030.9668728880520484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92360.6469635582155125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66000.4623169645108687No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.14275787479896218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.13056951846185744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.13007918228737622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17950.12573620474197109No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15520.10871453468498003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109250.037.535751
GGTATCA63800.035.005231
TCAACGC130400.031.0822144
ATCAACG131000.030.9398563
TATCAAC132500.030.7699762
CAACGCA132300.030.6031365
AACGCAG135100.030.038176
ACGCAGA147600.027.495257
CGCAGAG148750.027.0929018
GCAGAGT153450.025.6436639
GTACATG82250.025.3620511
GAGTACT77150.025.22210512-13
GTATAGG5300.025.103711
ACATGGG84350.023.5467913
TACATGG87700.023.4608672
AGTACTT81550.023.27856312-13
GTACTTT83700.022.99084314-15
CCCTATA2606.220944E-1021.9312652
CATGGGG33900.021.0248094
AGAGTAC138950.020.67469810-11
Produced by FastQC (version 0.11.5)