##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936778_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427592 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.974551552544426 33.0 31.0 34.0 30.0 34.0 2 32.100055197843645 33.0 31.0 34.0 30.0 34.0 3 32.1013699992715 34.0 31.0 34.0 30.0 34.0 4 35.699439335608496 37.0 35.0 37.0 35.0 37.0 5 35.71000888208956 37.0 35.0 37.0 35.0 37.0 6 35.787334196325 37.0 35.0 37.0 35.0 37.0 7 35.73415933964326 37.0 35.0 37.0 35.0 37.0 8 35.69008582284014 37.0 35.0 37.0 33.0 37.0 9 37.34866894743036 39.0 37.0 39.0 34.0 39.0 10-11 37.193903089958475 39.0 37.0 39.0 34.0 39.0 12-13 37.134238634007474 39.0 37.0 39.0 33.5 39.0 14-15 38.30806245762095 40.0 38.0 41.0 33.5 41.0 16-17 38.25437625035724 40.0 38.0 41.0 33.0 41.0 18-19 38.25846600429254 40.0 38.0 41.0 33.5 41.0 20-21 38.21752818732523 40.0 38.0 41.0 34.0 41.0 22-23 38.117299270379775 40.0 38.0 41.0 33.5 41.0 24-25 37.97076335535643 40.0 38.0 41.0 33.0 41.0 26-27 38.03479915830293 40.0 38.0 41.0 33.0 41.0 28-29 37.975599120757195 40.0 38.0 41.0 33.0 41.0 30-31 37.79597111779836 40.0 37.0 41.0 33.0 41.0 32-33 37.56449706919064 40.0 37.0 41.0 33.0 41.0 34-35 37.38652430106081 40.0 36.0 41.0 33.0 41.0 36-37 37.19974824739842 40.0 36.0 41.0 32.0 41.0 38-39 37.03798949559818 39.0 36.0 41.0 31.0 41.0 40-41 36.850821873476455 39.0 35.5 41.0 31.0 41.0 42-43 36.61456144332554 39.0 35.0 41.0 31.0 41.0 44-45 36.311313036217626 39.0 35.0 41.0 30.0 41.0 46-47 35.89169314482009 39.0 35.0 40.5 29.5 41.0 48-49 35.51653623724425 39.0 35.0 40.0 27.5 41.0 50-51 34.84102425623007 38.0 34.0 39.5 26.5 40.5 52-53 35.05784075562205 38.5 35.0 40.0 27.0 40.5 54-55 35.426920646795445 39.0 35.0 40.5 28.0 41.0 56-57 35.32739746370112 39.0 35.0 41.0 27.0 41.0 58-59 35.096649462871746 38.5 35.0 40.5 26.5 41.0 60-61 34.827741399503495 38.0 35.0 40.0 26.0 41.0 62-63 34.51192112312201 38.0 34.0 40.0 26.0 41.0 64-65 34.19406279945531 37.0 34.0 40.0 25.5 41.0 66-67 33.83970630264109 37.0 34.0 39.5 24.5 41.0 68-69 33.465577700071165 36.0 34.0 39.0 23.0 41.0 70-71 33.06097505449736 36.0 33.0 39.0 22.5 40.5 72-73 32.676878617980485 35.0 33.0 38.5 22.0 40.0 74-75 32.25887963787973 35.0 32.5 37.0 20.5 39.5 76-77 31.842961784599524 35.0 32.0 37.0 19.5 39.0 78-79 31.41419151970591 35.0 32.0 36.5 18.0 39.0 80-81 31.00234380691402 35.0 31.0 36.0 14.5 37.5 82-83 30.611473726386812 35.0 31.0 35.5 9.5 37.0 84-85 30.270499204254435 34.5 31.0 35.0 7.0 37.0 86-87 29.949820747104212 34.0 31.0 35.0 4.0 36.0 88-89 29.61103347455015 34.0 30.0 35.0 2.0 36.0 90-91 29.27484638468134 34.0 30.0 35.0 2.0 36.0 92-93 28.935712024163767 34.0 29.5 35.0 2.0 35.0 94-95 28.546853372672302 34.0 29.0 35.0 2.0 35.0 96-97 28.098022404160293 34.0 29.0 35.0 2.0 35.0 98-99 27.54862418674243 34.0 28.5 35.0 2.0 35.0 100-101 25.431181668151687 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3186996145225187 1101 2 0.3583283057856903 1101 3 0.24770781224913918 1101 4 0.2630552121534535 1101 5 0.2333191148235514 1101 6 0.133537573153113 1101 7 0.2745834191837915 1101 8 0.23364342349904632 1101 9 0.14039154422059852 1101 10-11 0.3022597944454972 1101 12-13 0.30445561546122946 1101 14-15 0.4944796059364833 1101 16-17 0.5315126413752296 1101 18-19 0.39409117410696837 1101 20-21 0.4662563567883282 1101 22-23 0.4614750176314928 1101 24-25 0.5383928175748736 1101 26-27 0.4920441216547715 1101 28-29 0.5584223551616176 1101 30-31 0.568524622793305 1101 32-33 0.6654076752085913 1101 34-35 0.6009616217871852 1101 36-37 0.6185529240423193 1101 38-39 0.5223234476988736 1101 40-41 0.4767226415646775 1101 42-43 0.5226863021043613 1101 44-45 0.40899578347566035 1101 46-47 0.5525398824893344 1101 48-49 0.576035292569351 1101 50-51 0.3795179700974671 1101 52-53 0.4194783544672802 1101 54-55 0.49924262731674673 1101 56-57 0.5206162553378206 1101 58-59 0.3529145219369809 1101 60-61 0.43641885773737243 1101 62-63 0.42902904292101596 1101 64-65 0.4821417784253157 1101 66-67 0.5792015247725644 1101 68-69 0.49113524242725504 1101 70-71 0.5083553881860965 1101 72-73 0.5250018441923245 1101 74-75 0.3515622014802062 1101 76-77 0.41164090770254447 1101 78-79 0.43088625466252495 1101 80-81 0.2507985287920995 1101 82-83 0.35662300200265307 1101 84-85 0.42123986140846625 1101 86-87 0.46208138201106763 1101 88-89 0.5685375366245431 1101 90-91 0.7075466163809345 1101 92-93 0.6858294881244262 1101 94-95 0.537924213051852 1101 96-97 0.49902688809105555 1101 98-99 0.618369609361018 1101 100-101 0.837739363932215 1102 1 0.36615093890729256 1102 2 0.18102620576356543 1102 3 0.22307182862732589 1102 4 0.15423848847519395 1102 5 0.08373883962823925 1102 6 0.07924036925872002 1102 7 0.08644583602677613 1102 8 0.10273699259769131 1102 9 0.1984208243279184 1102 10-11 0.33022568345531766 1102 12-13 0.38951681619487744 1102 14-15 0.36826856256727325 1102 16-17 0.45389091048033237 1102 18-19 0.2979730373764866 1102 20-21 0.3411900331233184 1102 22-23 0.2807401393408142 1102 24-25 0.20980751645249285 1102 26-27 0.30890329524033433 1102 28-29 0.2009130318753094 1102 30-31 0.22768208699751113 1102 32-33 0.18096340106824726 1102 34-35 0.16553679303773805 1102 36-37 -0.0024317972636538343 1102 38-39 0.11078549468277998 1102 40-41 0.07969874019008216 1102 42-43 0.2653875920543314 1102 44-45 0.188141690015307 1102 46-47 0.26610277423909423 1102 48-49 0.5387164291006457 1102 50-51 0.6111219343626004 1102 52-53 0.5693874431481944 1102 54-55 0.4361092298432112 1102 56-57 0.602590234022415 1102 58-59 0.5220539350575564 1102 60-61 0.48131061509959494 1102 62-63 0.5338718700749041 1102 64-65 0.5311729609904958 1102 66-67 0.2687532648259108 1102 68-69 0.13224783679500973 1102 70-71 0.24103623819225817 1102 72-73 0.24725801216371934 1102 74-75 0.3953101021657588 1102 76-77 0.33338278031346746 1102 78-79 0.3200999295752496 1102 80-81 0.3448084618936562 1102 82-83 0.23335298382039227 1102 84-85 0.23315682159079287 1102 86-87 0.30096605357213235 1102 88-89 0.4355310145529607 1102 90-91 0.48499581081378196 1102 92-93 0.5109580188131666 1102 94-95 0.5307579777263385 1102 96-97 0.5216069675517261 1102 98-99 0.40003676450126235 1102 100-101 0.31764687499881816 1103 1 0.1201688107079697 1103 2 0.11599614581648154 1103 3 0.13136678418975833 1103 4 0.09758166610277641 1103 5 0.08893932279933381 1103 6 -0.052520181100113916 1103 7 -0.09201710616722636 1103 8 0.029989674145426193 1103 9 -0.054391366237808825 1103 10-11 0.03797335616393127 1103 12-13 -9.580082754254704E-4 1103 14-15 0.05047020527987911 1103 16-17 0.08192623934255039 1103 18-19 0.012377100437568345 1103 20-21 0.23268010444116527 1103 22-23 0.20579090168318714 1103 24-25 0.19110727434851782 1103 26-27 0.2990482292881751 1103 28-29 0.3610389743267959 1103 30-31 0.27827127578503763 1103 32-33 0.22662738978733898 1103 34-35 0.21205652684432863 1103 36-37 0.05816452849319376 1103 38-39 0.1821615818609459 1103 40-41 0.14771976055204306 1103 42-43 0.1664826761859004 1103 44-45 0.3798709275692431 1103 46-47 0.21194354551564487 1103 48-49 0.265204780979154 1103 50-51 0.09758623137112465 1103 52-53 0.09970072672435037 1103 54-55 0.0955314982243678 1103 56-57 0.14555582622671182 1103 58-59 0.15684761097693922 1103 60-61 0.01227590316185001 1103 62-63 0.34321300511223285 1103 64-65 0.2259195985793454 1103 66-67 0.2499545221767292 1103 68-69 0.23992915124157577 1103 70-71 0.06317760548351004 1103 72-73 0.10813013944716232 1103 74-75 -0.07274979999036191 1103 76-77 0.22415656257888017 1103 78-79 0.3118241556979413 1103 80-81 0.27033340931683014 1103 82-83 0.420413122723442 1103 84-85 0.18028069207467468 1103 86-87 0.20877783803911498 1103 88-89 0.3199496107646631 1103 90-91 0.5047702272844568 1103 92-93 0.4052128449078616 1103 94-95 0.4991956381137399 1103 96-97 0.73888828338076 1103 98-99 0.5770780154927486 1103 100-101 0.6455285775721009 1104 1 0.383326983543558 1104 2 0.2383865714327129 1104 3 0.24675255118842898 1104 4 0.27099496209672225 1104 5 0.23300773138387143 1104 6 0.23696226322103797 1104 7 0.3153450947433569 1104 8 0.3148311138255764 1104 9 0.2958257130115314 1104 10-11 0.4210129940422789 1104 12-13 0.4613115927038578 1104 14-15 0.535766938963711 1104 16-17 0.5642807346002741 1104 18-19 0.5536873256187675 1104 20-21 0.5640355067643128 1104 22-23 0.4862379176140692 1104 24-25 0.5398589585840696 1104 26-27 0.4895108326540054 1104 28-29 0.41379055288482647 1104 30-31 0.5060494938858398 1104 32-33 0.5395649607894271 1104 34-35 0.4885010065146176 1104 36-37 0.5139243939304805 1104 38-39 0.5850888493043414 1104 40-41 0.5470240501789121 1104 42-43 0.45579691711304093 1104 44-45 0.5358333415961738 1104 46-47 0.5726288642654893 1104 48-49 0.7284157925772661 1104 50-51 0.6764753844538234 1104 52-53 0.7593354347461556 1104 54-55 0.7089261245222147 1104 56-57 0.6551275680565141 1104 58-59 0.6416978571175065 1104 60-61 0.7454648105443056 1104 62-63 0.7622763499820309 1104 64-65 0.8187573043202789 1104 66-67 0.4904994659474795 1104 68-69 0.5884721223651965 1104 70-71 0.5842770040022529 1104 72-73 0.6973193286363468 1104 74-75 0.720936848534997 1104 76-77 0.6288319124155173 1104 78-79 0.7404847811327571 1104 80-81 0.6693849535296685 1104 82-83 0.6542237662115049 1104 84-85 0.6293221088580232 1104 86-87 0.5770763418214919 1104 88-89 0.6116662540942137 1104 90-91 0.6478417445495204 1104 92-93 0.6750313437586222 1104 94-95 0.7258061195629324 1104 96-97 0.6517219381499011 1104 98-99 0.6619458745541529 1104 100-101 0.768305606800741 1105 1 0.06925493341791977 1105 2 0.16147640913887074 1105 3 0.19457308792492256 1105 4 0.10818135677398288 1105 5 0.08688475091253167 1105 6 0.08638452348373704 1105 7 0.13709766338995877 1105 8 0.2808642602484923 1105 9 0.13862051756029103 1105 10-11 0.2252060421451958 1105 12-13 0.16500225047490602 1105 14-15 0.1860624842044487 1105 16-17 0.1437498504883905 1105 18-19 0.07117332562608425 1105 20-21 0.13690551440898702 1105 22-23 0.1910145578352953 1105 24-25 0.1526070903943193 1105 26-27 0.15441629841208737 1105 28-29 0.08841225131894959 1105 30-31 -0.22131313061442626 1105 32-33 -0.11301045262642617 1105 34-35 -0.03344981153851023 1105 36-37 -0.013920869115750634 1105 38-39 -0.262219421207341 1105 40-41 -0.13297415752940367 1105 42-43 -0.2065362443065979 1105 44-45 -0.34580389856725446 1105 46-47 -0.42891232369470345 1105 48-49 -0.08072215170075481 1105 50-51 -0.3135651432846345 1105 52-53 -0.25024015789886533 1105 54-55 -0.2715508811070464 1105 56-57 0.016635726641410997 1105 58-59 -0.015573726280749156 1105 60-61 -0.02434330451350064 1105 62-63 -0.03947351193835402 1105 64-65 -0.1654900977712188 1105 66-67 -0.11349002902861827 1105 68-69 0.10900520111995604 1105 70-71 -0.021738329235979847 1105 72-73 -0.009957558482192042 1105 74-75 0.060186573290202716 1105 76-77 0.0023983533997444795 1105 78-79 -0.14936865330045634 1105 80-81 0.023926766963075607 1105 82-83 0.12231731652186184 1105 84-85 -0.09407680771994364 1105 86-87 -0.1541991480410907 1105 88-89 -0.04253337793733181 1105 90-91 -0.11102625865465754 1105 92-93 -0.020272669145878552 1105 94-95 -0.053456517007866466 1105 96-97 0.04702906013181263 1105 98-99 0.23449601299954637 1105 100-101 0.3311131768555704 1106 1 0.09813129061306469 1106 2 0.16810302822099032 1106 3 0.07699163004629384 1106 4 0.0026724959841999407 1106 5 0.010155044442782923 1106 6 -0.0069844459190449015 1106 7 0.17630107835127973 1106 8 0.20604608570353378 1106 9 0.12805905132221795 1106 10-11 0.16768231529723465 1106 12-13 0.18752565206708738 1106 14-15 0.342604162928005 1106 16-17 0.33430711457140205 1106 18-19 0.27034818537354255 1106 20-21 0.3654872347353759 1106 22-23 0.16054482120274116 1106 24-25 0.2641434640840501 1106 26-27 0.2711958325710455 1106 28-29 0.31839294533252627 1106 30-31 0.2809885213110519 1106 32-33 0.32366729516800774 1106 34-35 0.17294180090016908 1106 36-37 0.2170634445833457 1106 38-39 0.24358602050869393 1106 40-41 0.23544174964633413 1106 42-43 0.30368071251064777 1106 44-45 0.22780653025444764 1106 46-47 0.2944346479497213 1106 48-49 0.3675178780881936 1106 50-51 0.45530006548844426 1106 52-53 0.3767445565751686 1106 54-55 0.35082548244367473 1106 56-57 0.2286314586874738 1106 58-59 0.44777686430148833 1106 60-61 0.37264613551568715 1106 62-63 0.46872368129124453 1106 64-65 0.25470540711921075 1106 66-67 0.3684713604939631 1106 68-69 0.33923282782203046 1106 70-71 0.35452072838962323 1106 72-73 0.5097262106564884 1106 74-75 0.45983320345344936 1106 76-77 0.5520512643641489 1106 78-79 0.4916386974800453 1106 80-81 0.4889642012857074 1106 82-83 0.4288906983369998 1106 84-85 0.3809820560980839 1106 86-87 0.39859509814687755 1106 88-89 0.43357490307468183 1106 90-91 0.5417295550119192 1106 92-93 0.32603593951459686 1106 94-95 0.43033882906801324 1106 96-97 0.4475127565926549 1106 98-99 0.7327437442470526 1106 100-101 0.9012407518905832 1107 1 0.058224608441186376 1107 2 0.0877723208184591 1107 3 0.040842095277973556 1107 4 0.05345469455645002 1107 5 0.15431540269154453 1107 6 0.012515088773859873 1107 7 0.09391457331283704 1107 8 0.16664656564103097 1107 9 0.23582025300606801 1107 10-11 0.24281206584135617 1107 12-13 0.08050351189577754 1107 14-15 0.13933641753318682 1107 16-17 0.21024715556669094 1107 18-19 0.11522841215985125 1107 20-21 0.220539738268549 1107 22-23 0.13642219279834933 1107 24-25 0.3259768947020021 1107 26-27 0.1220865902297561 1107 28-29 0.16006409915836883 1107 30-31 0.11116142399776408 1107 32-33 0.024433920658388786 1107 34-35 0.06608085909447681 1107 36-37 -0.020732990726898493 1107 38-39 -0.37819943898394826 1107 40-41 0.0370595402144005 1107 42-43 -0.06433685302072689 1107 44-45 0.0017560825189164575 1107 46-47 -0.17538505874843935 1107 48-49 -0.026334412172936084 1107 50-51 -0.21163545365701708 1107 52-53 -0.16296868755797078 1107 54-55 -0.01668812609203485 1107 56-57 -0.11690355397460905 1107 58-59 -0.17326255784290368 1107 60-61 -0.09780573272623627 1107 62-63 -0.04547685777117749 1107 64-65 -0.04176685620388554 1107 66-67 -0.1508123003642865 1107 68-69 -0.1096646507715775 1107 70-71 -0.17844480871955426 1107 72-73 -0.0178526615356418 1107 74-75 -0.119630992032846 1107 76-77 -0.1360261274425234 1107 78-79 -0.02751958687161249 1107 80-81 -0.12995984581513298 1107 82-83 0.017007003994741865 1107 84-85 0.03579904033495751 1107 86-87 0.05384401858916732 1107 88-89 -0.032368689940732054 1107 90-91 0.15703502874280773 1107 92-93 -0.16526349653621963 1107 94-95 -0.2887174949606859 1107 96-97 -0.1922720558440929 1107 98-99 -0.16641885381057264 1107 100-101 -0.4552065417114086 1108 1 0.22916339920811524 1108 2 0.2087480213613162 1108 3 0.21232984076317507 1108 4 0.2528687518635735 1108 5 0.17259786257170617 1108 6 0.12874061063273956 1108 7 0.24742561574813493 1108 8 0.2657149065935229 1108 9 0.09638731853812743 1108 10-11 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16276.0 18 13907.0 19 7715.0 20 6849.0 21 6983.0 22 7410.0 23 8067.0 24 9303.0 25 10816.0 26 12762.0 27 15216.0 28 18158.0 29 22481.0 30 28244.0 31 35866.0 32 46290.0 33 62601.0 34 88504.0 35 133915.0 36 200822.0 37 296930.0 38 276138.0 39 50856.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52788000930595 19.52869007542865 15.172622568049759 21.770807347215644 2 15.031396871734968 21.0466604954406 40.61706187506701 23.30488075775742 3 18.00482185684228 26.898702470693664 30.956853419615747 24.13962225284831 4 12.241098033059844 17.611570664925004 38.59633017236305 31.551001129652107 5 12.93027858985744 37.92023808439071 34.30278239403499 14.846700931716853 6 29.28747207734724 37.75717402778002 18.177848461038657 14.77750543383409 7 26.751336992100704 34.360311485866085 21.838145103069298 17.05020641896391 8 26.142311124132693 33.91287952331654 20.47668345843634 19.468125894114426 9 27.00089226824682 16.52224257219978 21.14079964898041 35.33606551057299 10-11 23.886075501283987 27.236497112213097 27.84882219160577 21.02860519489714 12-13 25.588492893925306 25.255470666391428 28.287963640225243 20.868072799458023 14-15 22.876489958071602 25.752110788537045 27.400439329986725 23.970959923404624 16-17 20.88082099039711 29.297997984388118 27.773638936351237 22.047542088863533 18-19 20.893963390704716 27.177986769075517 31.2676599203902 20.660389919829566 20-21 21.757483458282493 26.290520287038483 31.319791119860785 20.63220513481824 22-23 21.637725814842774 26.248596457730827 31.171898675196235 20.941779052230164 24-25 21.2298408919662 26.710329919463405 31.254021838165258 20.805807350405132 26-27 21.13214262070038 26.670150836288283 31.34309484635968 20.85461169665166 28-29 20.762136692645946 26.525487168403387 31.76563231794742 20.946743821003246 30-31 20.951468553514196 26.62279979683696 31.478676638001996 20.94705501164685 32-33 20.867655192246193 26.75873804142196 31.212392191971933 21.161214574359914 34-35 21.26633054587054 26.70958897008189 31.00153909418318 21.022541389864397 36-37 21.54310326408851 26.393264680447448 30.890961559544373 21.17267049591967 38-39 21.068275554076656 26.795506090245976 30.96752160701933 21.168696748658043 40-41 21.007615117309093 26.68047645884611 31.29732302759495 21.01458539624985 42-43 21.075182571233427 26.857498117370977 30.674173832332187 21.393145479063413 44-45 21.10935093709711 26.93815765002643 30.40733104109876 21.545160371777705 46-47 21.41729845519736 27.127562324832354 29.86481838604445 21.590320833925837 48-49 21.469036969167785 27.32642584679053 29.493447484434338 21.711089699607342 50-51 21.43835865937755 27.44172394565105 29.366921281442732 21.752996113528674 52-53 21.561072929414493 27.66931155960886 29.17712469991895 21.592490811057694 54-55 21.308720953447757 27.500239931124003 29.23212232914627 21.958916786281964 56-57 21.376592649265273 27.942979031380588 28.923887383131568 21.756540936222564 58-59 21.394402787852847 27.439397511065838 29.189581489318407 21.97661821176291 60-61 21.615870970770363 27.438012996944305 29.291116209188377 21.65499982309695 62-63 21.6292738972198 27.43175600648725 29.460843552856037 21.47812654343691 64-65 21.519629803857832 27.412721691928915 29.310218608654047 21.75742989555921 66-67 21.37979060878143 27.684576453576355 29.020944726547178 21.914688211095044 68-69 21.319477214581013 27.643328257100507 29.33442495110316 21.702769577215317 70-71 21.47038516226236 27.645466178717164 29.149879828365272 21.734268830655203 72-73 21.544011296327888 27.623494036044704 28.839930038991383 21.992564628636025 74-75 21.311090653562626 28.07136461656809 28.669187428976578 21.948357300892706 76-77 21.47113793012848 27.78276595931022 28.917385554514325 21.82871055604698 78-79 21.40301261193588 27.73140363975768 28.83255243761739 22.03303131068905 80-81 21.25588530921018 28.190942045360188 28.677146799992855 21.87602584543678 82-83 21.39202824598079 28.08072442336319 28.60847975005194 21.918767580604083 84-85 21.358054013607443 27.841662655616485 28.37854785270443 22.421735478071643 86-87 21.388522710513254 27.752763211410237 28.719112129053908 22.139601949022598 88-89 21.601439332291058 27.475349928205034 28.714763279791583 22.20844745971232 90-91 21.35886891819512 27.57342934399371 28.946226253492945 22.121475484318225 92-93 21.43313085988272 27.78487411915641 28.700909413859332 22.081085607101542 94-95 21.220684907752375 27.490265523927633 28.740517315369 22.548532252950995 96-97 21.417324876112744 27.497890833464133 28.502617889095678 22.582166401327445 98-99 21.251555438436526 27.62181829395342 28.462832599256334 22.66379366835372 100-101 21.40381716761691 27.48783326446998 28.556038825615204 22.552310742297905 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1776.0 1 1200.5 2 845.5 3 1014.0 4 791.0 5 813.5 6 1638.0 7 3632.0 8 6256.5 9 6808.5 10 4802.0 11 3214.5 12 3019.5 13 3138.0 14 3119.0 15 2990.5 16 2926.0 17 2898.0 18 2916.5 19 3099.5 20 3433.5 21 3841.0 22 4375.5 23 5099.5 24 5887.5 25 7219.0 26 9322.0 27 11419.0 28 12824.0 29 14498.5 30 17054.5 31 20000.0 32 23924.0 33 28634.5 34 33567.5 35 36579.5 36 40354.5 37 44902.0 38 45981.0 39 45231.0 40 44226.5 41 44450.0 42 45112.0 43 46207.0 44 47976.0 45 49774.0 46 49145.5 47 47849.0 48 52916.5 49 52846.0 50 46773.0 51 43496.5 52 40928.0 53 40288.5 54 39490.5 55 37515.5 56 35092.0 57 32004.0 58 29172.0 59 26591.0 60 23076.5 61 19427.0 62 15998.5 63 12425.5 64 9417.5 65 7120.5 66 5132.5 67 4018.5 68 2919.5 69 1762.5 70 1210.5 71 911.0 72 690.5 73 469.5 74 324.0 75 242.0 76 151.5 77 78.5 78 50.0 79 32.0 80 24.0 81 19.5 82 13.5 83 9.0 84 6.5 85 5.0 86 6.5 87 6.5 88 4.0 89 5.5 90 6.0 91 3.0 92 1.5 93 3.0 94 2.5 95 1.0 96 1.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03838631765938728 2 0.03775588543505427 3 0.023886376499728215 4 0.04230900705523707 5 0.06101182971044949 6 0.06185240600956015 7 0.061782357984634266 8 0.06311327045822616 9 0.06241279020896726 10-11 0.06868208843983435 12-13 0.06728112794131658 14-15 0.061221973785227146 16-17 0.051135058195899113 18-19 0.04427035175316197 20-21 0.05218577856978744 22-23 0.027984185957892732 24-25 0.01600597369556568 26-27 0.012853812573900667 28-29 0.016566357894972794 30-31 0.011698020162623494 32-33 0.007880402804162533 34-35 0.009036195215439706 36-37 0.005078481807126966 38-39 0.0073200186047554195 40-41 0.007179922554903642 42-43 0.004693217670034576 44-45 0.00129588846112895 46-47 0.002171488772702565 48-49 7.355042617218365E-4 50-51 0.005323649894367579 52-53 0.00427292952047924 54-55 0.006934754467663029 56-57 0.020489047290822588 58-59 0.007950450829088422 60-61 0.01796731839349058 62-63 0.012398500411882387 64-65 0.009281363302680317 66-67 0.015130373383992065 68-69 0.02469192878637594 70-71 0.018107414443342355 72-73 0.015795829620788012 74-75 0.01723181413176874 76-77 0.023080824213080487 78-79 0.03442860425107454 80-81 0.029104954356706958 82-83 0.029104954356706958 84-85 0.01996368710387842 86-87 0.021890007789340374 88-89 0.018457654567971802 90-91 0.01754703024393524 92-93 0.028649642194688678 94-95 0.022380343963821596 96-97 0.03418343616383392 98-99 0.05208070653239861 100-101 0.05978598927424642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427592.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.60348665072286 #Duplication Level Percentage of deduplicated Percentage of total 1 71.07823663142989 32.414154153783336 2 14.677702478849817 13.387088181150187 3 5.7554088376397115 7.8740013029026485 4 2.6872344453694312 4.9018904062707005 5 1.530620461013293 3.4900814880571485 6 0.9404754323515426 2.573337529474583 7 0.6351164649244695 2.0274467660886133 8 0.4448020220005882 1.6227618458014688 9 0.31835816849692106 1.3066418238478126 >10 1.6725785733667953 14.234511727096292 >50 0.16635298919252967 5.258564744349103 >100 0.08880413221223103 7.243051468658831 >500 0.0027703048839485406 0.7786481791877624 >1k 0.0010773407882022103 0.8075649855538485 >5k 3.078116537720601E-4 1.111472198090626 >10k+ 1.5390582688603005E-4 0.9687831996870997 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13803 0.9668728880520484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9236 0.6469635582155125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6600 0.4623169645108687 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.14275787479896218 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1864 0.13056951846185744 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1857 0.13007918228737622 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1795 0.12573620474197109 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1552 0.10871453468498003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.4009604985177837E-4 7 0.0 0.0 0.0 0.0 1.4009604985177837E-4 8 0.0 0.0 0.0 0.0 2.1014407477766756E-4 9 0.0 0.0 0.0 0.0 2.1014407477766756E-4 10-11 0.0 0.0 0.0 7.004802492588918E-5 2.1014407477766756E-4 12-13 0.0 0.0 0.0 7.004802492588918E-5 2.1014407477766756E-4 14-15 0.0 0.0 0.0 7.004802492588918E-5 2.451680872406121E-4 16-17 0.0 0.0 0.0 1.0507203738883378E-4 3.5024012462944596E-4 18-19 0.0 0.0 0.0 1.4009604985177837E-4 4.2028814955533513E-4 20-21 0.0 0.0 0.0 2.1014407477766756E-4 4.2028814955533513E-4 22-23 0.0 0.0 0.0 4.903361744812244E-4 4.5531216201827974E-4 24-25 0.0 0.0 0.0 8.055522866477257E-4 4.903361744812244E-4 26-27 0.0 0.0 0.0 0.0012608644486660054 4.903361744812244E-4 28-29 0.0 0.0 0.0 0.0024516808724061217 4.903361744812244E-4 30-31 0.0 0.0 0.0 0.004973409769738132 4.903361744812244E-4 32-33 0.0 0.0 0.0 0.008756003115736148 6.304322243330027E-4 34-35 0.0 0.0 0.0 0.012538596461734164 6.304322243330027E-4 36-37 0.0 0.0 0.0 0.01775717431871291 6.304322243330027E-4 38-39 0.0 0.0 0.0 0.02259048803859926 6.304322243330027E-4 40-41 0.0 0.0 0.0 0.029700362568577016 6.304322243330027E-4 42-43 0.0 0.0 0.0 0.040627854457015736 6.654562367959473E-4 44-45 0.0 0.0 0.0 0.05530291567898951 7.004802492588919E-4 46-47 0.0 0.0 0.0 0.08531849435973303 7.70528274184781E-4 48-49 0.0 0.0 0.0 0.13284607927194886 8.405762991106703E-4 50-51 0.0 0.0 0.0 0.17634590275092604 8.405762991106703E-4 52-53 0.0 0.0 0.0 0.21918026999310727 9.106243240365595E-4 54-55 0.0 0.0 0.0 0.27105083245072825 9.806723489624487E-4 56-57 0.0 0.0 0.0 0.3208199541605725 9.806723489624487E-4 58-59 0.0 0.0 0.0 0.3840382966561875 9.806723489624487E-4 60-61 0.0 0.0 0.0 0.467150278230755 0.0010507203738883378 62-63 0.0 0.0 0.0 0.5420666408889936 0.0010507203738883378 64-65 0.0 0.0 0.0 0.6336894574920566 0.0010507203738883378 66-67 0.0 0.0 0.0 0.7451358651491462 0.0010857443863512824 68-69 0.0 0.0 0.0 0.8789275927575946 0.0011557924112771717 70-71 0.0 0.0 0.0 1.020984987307298 0.0012258404362030608 72-73 0.0 0.0 0.0 1.1715882408979597 0.001471008523443673 74-75 0.0 0.0 0.0 1.3164125324322353 0.001471008523443673 76-77 0.0 0.0 0.0 1.4630580726145845 0.001471008523443673 78-79 0.0 0.0 0.0 1.6254293943927958 0.001471008523443673 80-81 0.0 0.0 0.0 1.8206882638737119 0.0015410565483695623 82-83 0.0 0.0 0.0 2.0478540087083705 0.0016811525982213405 84-85 0.0 0.0 0.0 2.2867878217305786 0.0016811525982213405 86-87 0.0 0.0 0.0 2.58999069762229 0.0016811525982213405 88-89 0.0 0.0 0.0 2.935572628594164 0.0016811525982213405 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10925 0.0 37.53575 1 GGTATCA 6380 0.0 35.00523 1 TCAACGC 13040 0.0 31.082214 4 ATCAACG 13100 0.0 30.939856 3 TATCAAC 13250 0.0 30.769976 2 CAACGCA 13230 0.0 30.603136 5 AACGCAG 13510 0.0 30.03817 6 ACGCAGA 14760 0.0 27.49525 7 CGCAGAG 14875 0.0 27.092901 8 GCAGAGT 15345 0.0 25.643663 9 GTACATG 8225 0.0 25.362051 1 GAGTACT 7715 0.0 25.222105 12-13 GTATAGG 530 0.0 25.10371 1 ACATGGG 8435 0.0 23.546791 3 TACATGG 8770 0.0 23.460867 2 AGTACTT 8155 0.0 23.278563 12-13 GTACTTT 8370 0.0 22.990843 14-15 CCCTATA 260 6.220944E-10 21.931265 2 CATGGGG 3390 0.0 21.024809 4 AGAGTAC 13895 0.0 20.674698 10-11 >>END_MODULE