FastQCFastQC Report
Thu 26 May 2016
SRR936778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427592
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118820.8323106321694154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89520.6270699191365601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66370.4649087414331265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29400.20594119328211422No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.17329881366664987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.1425477307241845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.13596321638115091No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16300.11417828062919937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73100.036.3448521
GTATCAA112650.031.6430641
TCAACGC139500.025.261474
ATCAACG139700.025.194843
TATCAAC139800.025.1803442
CAACGCA141450.024.913225
AACGCAG144900.024.4183776
GAGTACT83000.023.74646412-13
GCACCGT3050.023.3571766
GTACATG85800.023.2653981
GTACTTT89150.022.10870214-15
AGTACTT88500.021.86821212-13
ACGCAGA161000.021.8290447
GTATAGG5750.021.490871
CGCAGAG162900.021.3995138
TACATGG91150.021.3686852
ACATGGG87800.021.0989023
GCAGAGT168050.020.4328379
CATGGGG35300.019.9120464
ACTTTTT101000.019.67968816-17
Produced by FastQC (version 0.11.5)